Effect of Tobacco Smoking on The Clinical, Histopathological, and Serological Manifestations of Sjögren’s Syndrome

The authors wish to thank Dr. A. Darise Farris for her critical review of the cellular immune response discussion.Objectives To assess the association of smoking habits with the clinical, serological, and histopathological manifestations of Sjögren’s syndrome (SS) and non-Sjögren’s sicca (non-SS sicca). Methods Cross-sectional case-control study of 1288 patients with sicca symptoms (587 SS and 701 non-SS sicca) evaluated in a multi-disciplinary research clinic. Smoking patterns were obtained from questionnaire data and disease-related clinical and laboratory data were compared between current, past, ever, and never smokers. Results Current smoking rates were 4.6% for SS patients compared to 14.1% in non-SS sicca (p = 5.17x10E-09), 18% in a local lupus cohort (p = 1.13x10E-14) and 16.8% in the community (p = 4.12x10E-15). Current smoking was protective against SS classification (OR 0.35, 95%CI 0.22–0.56, FDR q = 1.9E10-05), focal lymphocytic sialadenitis (OR 0.26, 95%CI 0.15–0.44, FDR q = 1.52x10E-06), focus score ≥1 (OR 0.22, 95%CI 0.13–0.39, FDR q = 1.43x10E-07), and anti-Ro/SSA(+) (OR 0.36, 95%CI 0.2–0.64, FDR q = 0.0009); ever smoking was protective against the same features and against anti-La/SSB(+) (OR 0.52, 95%CI 0.39–0.70, FDR q = 5.82x10E-05). Duration of smoking was inversely correlated with SS even after controlling for socioeconomic status, BMI, alcohol and caffeine consumption. Conclusions Current tobacco smoking is negatively and independently associated with SS, protecting against disease-associated humoral and cellular autoimmunity. The overall smoking rate amongst SS patients is significantly lower than in matched populations and the effects of smoking are proportional to exposure duration. In spite of the protective effects of tobacco on SS manifestations, it is associated with other serious comorbidities such as lung disease, cardiovascular risk and malignancy, and should thus be strongly discouraged in patients with sicca.Yeshttp://www.plosone.org/static/editorial#pee

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Systematic Study of Legacy Phosphorus (P) Desorption Mechanisms in High-P Agricultural Soils

Repeated manure additions containing phosphorus (P) in excess of crop needs have led to many agricultural soils with high levels of soil P (i.e., legacy P), particularly in the Delmarva region (USA). Due to the potential for P release, it is important to gain a better understanding of the mechanisms of P desorption and solubilization. Agricultural soils with high legacy P were collected from the Delmarva Peninsula, and soil P pools were determined using a suite of wet chemical and spectroscopic techniques, including a modified Hedley sequential extraction and X-ray absorption near-edge structure (XANES) spectroscopy. Five different desorption solutions were used to investigate P removal efficiency to assess release mechanisms. The results indicate that sulfate can have a stronger competition for P desorption than silicate, especially in the ditch sample with 21% labile P and 44% P adsorbed to iron and aluminum (via Hedley extraction). Additionally, linear combination fitting results of the ditch sample indicate 10.5% organic P and 73.9% P associated with iron and aluminum. This is an important finding because sulfate is a prevalent ion in sea water, and many agricultural soils with high legacy P in the Delmarva coastal area are threatened by sea level rise and inundation

Soil Functions: Connecting Earth's Critical Zone

Soil is the central interface of Earth's critical zone—the planetary surface layer extending from unaltered bedrock to the vegetation canopy—and is under intense pressure from human demand for biomass, water, and food resources. Soil functions are flows and transformations of mass, energy, and genetic information that connect soil to the wider critical zone, transmitting the impacts of human activity at the land surface and providing a control point for beneficial human intervention. Soil functions are manifest during bedrock weathering and, in fully developed soil profiles, correlate with the porosity architecture of soil structure and arise from the development of soil aggregates as fundamental ecological units. Advances in knowledge on the mechanistic processes of soil functions, their connection throughout the critical zone, and their quantitative representation in mathematical and computational models define research frontiers that address the major global challenges of critical zone resource provisioning for human benefit. ▪ Connecting the mechanisms of soil functions with critical zone processes defines integrating science to tackle challenges of climate change and food and water supply. ▪ Soil functions, which develop through formation of soil aggregates as fundamental eco-logical units, are manifest at the earliest stages of critical zone evolution. ▪ Global degradation of soil functions during the Anthropocene is reversible through positive human intervention in soil as a central control point in Earth's critical zone. ▪ Measurement and mathematical translation of soil functions and critical zone processes offer new computational approaches for basic and applied geosciences research