Effect of chrysin endothelial vasodilation on L-NAME induced hypertensive rats

572-577Hypertension is the major risk factor for cardiovascular diseases and stroke and it is one of leading causes of disability and mortality worldwide. The present study was aimed to investigate the antihypertensive and vasodilation properties of chrysin, a naturally occurring flavone, in hypertensive rats. Hypertension was induced using Nω-nitro-l arginine methyl ester (L-NAME; 40 mg/kg BWT/day for 4 weeks) in adult rats and chrysin (25 mg/kg BWT/day) or vehicle was administered for 4 weeks. A siginificant increase of blood pressure and serum fibrinogen along with a significant reduction of bilirubin level were observed in hypertensive rats and these changes were significantly attenuated upon administration of crysin. Further, L-NAME induced increase of tissue levels of mRNAs of β-MHC and TGF-β (in heart) as well as eNOS (in aorta, heart, kidney) were significantly reduced in chrysin treated rats. These results suggest that chrysin has a potential to attenuate impairment of organs (heart and renal) and endothelial function caused by L-NAME induced hypertension

Effect of chrysin endothelial vasodilation on L-NAME induced hypertensive rats

Hypertension is the major risk factor for cardiovascular diseases and stroke and it is one of leading causes of disability and mortality worldwide. The present study was aimed to investigate the antihypertensive and vasodilation properties of chrysin, a naturally occurring flavone, in hypertensive rats. Hypertension was induced using Nω-nitro-l arginine methyl ester (L-NAME; 40 mg/kg BWT/day for 4 weeks) in adult rats and chrysin (25 mg/kg BWT/day) or vehicle was administered for 4 weeks. A siginificant increase of blood pressure and serum fibrinogen along with a significant reduction of bilirubin level were observed in hypertensive rats and these changes were significantly attenuated upon administration of crysin. Further, L-NAME induced increase of tissue levels of mRNAs of β-MHC and TGF-β (in heart) as well as eNOS (in aorta, heart, kidney) were significantly reduced in chrysin treated rats. These results suggest that chrysin has a potential to attenuate impairment of organs (heart and renal) and endothelial function caused by L-NAME induced hypertension

The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic précis

<p>Abstract</p> <p>Background</p> <p>The final article in a series of three publications examining the global distribution of 41 dominant vector species (DVS) of malaria is presented here. The first publication examined the DVS from the Americas, with the second covering those species present in Africa, Europe and the Middle East. Here we discuss the 19 DVS of the Asian-Pacific region. This region experiences a high diversity of vector species, many occurring sympatrically, which, combined with the occurrence of a high number of species complexes and suspected species complexes, and behavioural plasticity of many of these major vectors, adds a level of entomological complexity not comparable elsewhere globally. To try and untangle the intricacy of the vectors of this region and to increase the effectiveness of vector control interventions, an understanding of the contemporary distribution of each species, combined with a synthesis of the current knowledge of their behaviour and ecology is needed.</p> <p>Results</p> <p>Expert opinion (EO) range maps, created with the most up-to-date expert knowledge of each DVS distribution, were combined with a contemporary database of occurrence data and a suite of open access, environmental and climatic variables. Using the Boosted Regression Tree (BRT) modelling method, distribution maps of each DVS were produced. The occurrence data were abstracted from the formal, published literature, plus other relevant sources, resulting in the collation of DVS occurrence at 10116 locations across 31 countries, of which 8853 were successfully geo-referenced and 7430 were resolved to spatial areas that could be included in the BRT model. A detailed summary of the information on the bionomics of each species and species complex is also presented.</p> <p>Conclusions</p> <p>This article concludes a project aimed to establish the contemporary global distribution of the DVS of malaria. The three articles produced are intended as a detailed reference for scientists continuing research into the aspects of taxonomy, biology and ecology relevant to species-specific vector control. This research is particularly relevant to help unravel the complicated taxonomic status, ecology and epidemiology of the vectors of the Asia-Pacific region. All the occurrence data, predictive maps and EO-shape files generated during the production of these publications will be made available in the public domain. We hope that this will encourage data sharing to improve future iterations of the distribution maps.</p

Effect of food on immature development, consumption rate, and relative growth rate of Toxorhynchites splendens (Diptera: Culicidae), a predator of container breeding mosquitoes

Food utilization by the larvae of Toxorhynchites splendens (Wiedemann) was studied in the laboratory by offering larvae of Aedes aegypti Linnaeus, Anopheles stephensi (Liston), and Culex quinquefasciatus (Say). Quantitative analyses of data indicated that immature development was significantly faster with increase in food availability. The regression analysis showed that the degrees of the relationship between immature duration (Id) and food availability were higher when offered early instars of prey (first and second instars) than late instars. Consumption rate (Cr) of the predator increased with increase in food availability and this relationship was highly significant when larvae of An. stephensi were offered as food. Consumption rate to food level decreased with increase in the age class of the prey. There was a significant negative correlation between Id and Cr. This aspect helps to increase population turnover of T. splendens in a shorter period when the prey is abundant. Conversely, the predator compensated the loss in daily food intake at low food level by extending Id thereby attains the minimum threshold pupal weight for adult emergence. There was an increase in the relative growth rate (RGR) of the predator when An. stephensi was offered as prey and this was related to the high protein content of the prey per body weight. There was a positive correlation between Cr and RGR. This adaptive life characteristic strategy of this predator is useful for mass-rearing for large scale field release programmes in the control of container breeding mosquitoes is discussed