2,423 research outputs found
Laser-light scattering approach to peptide–membrane interaction
© International University Line, 2010Membrane-active peptides are becoming widely used, mainly due to their high therapeutic potential. Although the therapeutic action is characterized, the mechanisms of interaction are often unclear or controversial. In biophysical studies, non-invasive techniques are overlooked when studying the effect of peptides on membranes. Light scattering techniques, such as dynamic light scattering and static light scattering, can be used as tools to determine whether promotion of membrane aggregation in the presence of peptides and of self-peptide aggregation in solution occurs. More recently, light scattering has been used for evaluating the alteration on membrane surface charge (ζ-potential) promoted by membrane–peptide interactions. The data obtained by these techniques (either by themselves or combined with complementary experimental approaches) therefore yield valuable elucidations of membrane-active peptides’ mechanisms of action at the molecular level.This work was partially supported by the Fundação para a Ciência e Tecnologia (FCT) of the Portuguese Ministry of Science, Technology and Higher Education. M.M.D. acknowledges the grant SFRH/BD/41750/2007 from FCT
Translocating the blood-brain barrier using electrostatics
Copyright © 2012 Ribeiro,Domingues,
Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.Mammalian cell membranes regulate homeostasis, protein activity, and cell signaling. The charge at the membrane surface has been correlated with these key events. Although mammalian cells are known to be slightly anionic, quantitative information on the membrane charge and the importance of electrostatic interactions in pharmacokinetics and pharmacodynamics remain elusive. Recently, we reported for the first time that brain endothelial cells (EC) are more negatively charged than human umbilical cord cells, using zeta-potential measurements by dynamic light scattering. Here, we hypothesize that anionicity is a key feature of the blood-brain barrier (BBB) and contributes to select which compounds cross into the brain. For the sake of comparison, we also studied the membrane surface charge of blood components—red blood cells (RBC), platelets, and peripheral blood mononuclear cells (PBMC).To further quantitatively correlate the negative zeta-potential values with membrane charge density, model membranes with different percentages of anionic lipids were also evaluated. From all the cells tested, brain cell membranes are the most anionic and those having their lipids mostly exposed, which explains why lipophilic cationic compounds are more prone to cross the blood-brain barrier.Fundação para a Ciência e Tecnologia — Ministério da Educação e Ciência (FCT-MEC, Portugal) is acknowledged for funding (including fellowships SFRH/BD/42158/2007 to Marta M.B. Ribeiro, SFRH/BD/41750/2007 to Marco M. Domingues and SFRH/BD/70423/2010 to João M. Freire) and project PTDC/QUI-BIQ/119509/2010. Marie Curie Industry-Academia Partnerships and Pathways (European Commission) is also acknowledged for funding (FP7-PEOPLE-2007-3-1-IAPP, Project 230654)
Pediatric ocular rosacea, a misdiagnosed disease with high morbidity: Proposed diagnostic criteria
Ocular rosacea is an important and underdiagnosed chronic inflammatory disorder observed in children.
A clinical spectrum ranging from chronic eyelid inflammation,
recurrent ocular redness, photophobia
and/or hordeola/chalazions and conjunctival/corneal
phlyctenules evolving to neovascularization and scarring
may occur. Visual impairment and consequent amblyopia
are frequent and corneal perforation although rare is
the most feared complication. Ocular manifestations
usually precede cutaneous lesions. Although few cases of
pediatric ocular rosacea (POR) have been reported in the
literature, many cases must have been underdiagnosed
or misdiagnosed. The delay in diagnosis is greater than
one year in the large majority of cases and may lead to
serious ocular sequelae. This review aims to highlight
the clinical features of POR, its epidemiology, easy
diagnosis and effective treatment. We also propose new
diagnostic criteria, in which at least three of the five
clinical criteria must be present: (1) Chronic or recurrent
keratoconjunctivitis and/or red eye and/or photophobia;
(2) Chronic or recurrent blepharitis and/or chalazia/
hordeola; (3) Eyelid telangiectasia documented by an
ophthalmologist; (4) Primary periorificial dermatitis and/
or primary features of rosacea; and (5) Positive familial
history of cutaneous and/or ocular rosacea
SAMplus: adaptive optics at optical wavelengths for SOAR
Adaptive Optics (AO) is an innovative technique that substantially improves
the optical performance of ground-based telescopes. The SOAR Adaptive Module
(SAM) is a laser-assisted AO instrument, designed to compensate ground-layer
atmospheric turbulence in near-IR and visible wavelengths over a large Field of
View. Here we detail our proposal to upgrade SAM, dubbed SAMplus, that is
focused on enhancing its performance in visible wavelengths and increasing the
instrument reliability. As an illustration, for a seeing of 0.62 arcsec at 500
nm and a typical turbulence profile, current SAM improves the PSF FWHM to 0.40
arcsec, and with the upgrade we expect to deliver images with a FWHM of
arcsec -- up to 0.23 arcsec FWHM PSF under good seeing
conditions. Such capabilities will be fully integrated with the latest SAM
instruments, putting SOAR in an unique position as observatory facility.Comment: To appear in Proc. SPIE 10703 (Ground-based and Airborne
Instrumentation for Astronomy VII; SPIEastro18
Tailoring: A case study on the application of the seventh principle of PMBOK 7 in a public institution
In the 7th edition of PMBOK the Tailoring process is legitimized as an essential ally in project management, since it makes several adjustments throughout the project's life cycle to provide the best possible environment to achieve the deliverables and the value added to the organization. Tailoring as a principle further highlights the unique nature of each project and the need to carry out this process continuously. Based on a unique case study, the beginning of the management functions in a sector of a public organization is discussed, as well as the adaptations made to optimize the workflow and productivity in the deliverables. From this investigation results the proposal of a framework adapted to the needs of the institution, as a starting point for the professionalization of project management.info:eu-repo/semantics/publishedVersio
An open and parallel multiresolution framework using block-based adaptive grids
A numerical approach for solving evolutionary partial differential equations
in two and three space dimensions on block-based adaptive grids is presented.
The numerical discretization is based on high-order, central finite-differences
and explicit time integration. Grid refinement and coarsening are triggered by
multiresolution analysis, i.e. thresholding of wavelet coefficients, which
allow controlling the precision of the adaptive approximation of the solution
with respect to uniform grid computations. The implementation of the scheme is
fully parallel using MPI with a hybrid data structure. Load balancing relies on
space filling curves techniques. Validation tests for 2D advection equations
allow to assess the precision and performance of the developed code.
Computations of the compressible Navier-Stokes equations for a temporally
developing 2D mixing layer illustrate the properties of the code for nonlinear
multi-scale problems. The code is open source
Virtual laboratories in (bio)chemical engineering education
In the last decades, Information and Communications Technologies (ICT) have been promoting the creation and adoption of new learning and teaching styles. Virtual laboratories, by overcoming some limitations of conventional hands-on experiments, have been adopted as a complement or in substitution of laboratory sessions.
This paper describes the design and implementation of two virtual labs for biochemical engineering education intended for students at the BSc degree.
One of the virtual labs is intended to fully replace the hands-on experiment and consists on the determination of the correlation between oxygen transfer rate, aeration rate and agitation power in a reactor. The other virtual lab consists on the determination of the residence time distribution (RTD) in continuous stirred tanks series and was implemented to support the physical experiments rather than replacing them.
The virtual labs provide the students a learning platform covering the fundamentals underlying the experiment, its pre-visualization and simulation. The effectiveness of the implemented system was evaluated through direct experimentation and survey (through questionnaires) with students taking the chemical technology lab course. For the RTD virtual Lab, and based on specific learning outcomes, teachers could assess significant improvement in students’ performance in the lab and also a more thorough discussion of the results in the reports. The survey results show that, in average, considering the two virtual labs and several classes, 93% of the students consider the virtual labs of great utility.Universidade do Minho (UM) - “Programa Qualidade
Lipoxidation and cancer immunity
Lipoxidation is a well-known reaction between electrophilic carbonyl species, formed during oxidation of lipids, and specific proteins that, in most cases, causes an alteration in proteins function. This can occur under physiological conditions but, in many cases, it has been associated to pathological process, including cancer. Lipoxidation may have an effect in cancer development through their effects in tumour cells, as well as through the alteration of immune components and the consequent modulation of the immune response. The formation of protein adducts affects different proteins in cancer, triggering different mechanism, such as proliferation, cell differentiation and apoptosis, among others, altering cancer progression. The divergent results obtained documented that the formation of lipoxidation adducts can have either anti-carcinogenic or pro-carcinogenic effects, depending on the cell type affected and the specific adduct formed. Moreover, lipoxidation adducts may alter the immune response, consequently causing either positive or negative alterations in cancer progression. Therefore, in this review, we summarize the effects of lipoxidation adducts in cancer cells and immune components and their consequences in the evolution of different types of cancer.publishe
Virtual laboratories in (bio)chemical engineering education
"Qualidade” of the Universidade do Minho
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