Two modes of Gulf Stream variability revealed in the last two decades of satellite altimeter data

Author Posting. © American Meteorological Society, 2014. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 44 (2014): 149–163, doi:10.1175/JPO-D-13-0136.1.Monthly mapped sea level anomalies (MSLAs) of the NW Atlantic in the region immediately downstream of the Gulf Stream (GS) separation point reveal a leading mode in which the path shifts approximately 100 km meridionally about a nominal latitude of 39°N, producing coherent sea level anomaly (SLA) variability from 72° to 50°W. This mode can be captured by use of a simple 16-point index based on SLA data taken along the maximum of the observed variability in the region 33°–46°N and 45°–75°W. The GS shifts between 2010 and 2012 are the largest of the last decade and equal to the largest of the entire record. The second group of EOF modes of variability describes GS meanders, which propagate mainly westward interrupted by brief periods of eastward or stationary meanders. These meanders have wavelengths of approximately 400 km and can be seen in standard EOFs by spatial phase shifting of a standing meander pattern in the SLA data. The spectral properties of these modes indicate strong variability at interannual and longer periods for the first mode and periods of a few to several months for the meanders. While the former is quite similar to a previous use of the altimeter for GS path, the simple index is a useful measure of the large-scale shifts in the GS path that is quickly estimated and updated without changes in previous estimates. The time-scale separation allows a low-pass filtered 16-point index to be reflective of large-scale, coherent shifts in the GS path.Agencia Canaria de Investigación, Innovación y Sociedad de la Información (ACIISI) grant program of Apoyo al Personal Investigador en Formación and NSF Grant OCE-07267202014-07-0

Longitudinal variability of size-fractionated N-2 fixation and DON release rates along 24.5 degrees N in the subtropical North Atlantic

Dinitrogen (N-2) fixation and dissolved organic nitrogen (DON) release rates were measured on fractionated samples (\u3e10 mu m and m) along 24.5 degrees N in the subtropical North Atlantic. Net N-2 fixation rates (N-2 assimilation into biomass) ranged from 0.01 to 0.4 nmol N L-1 h(-1), and DON release rates ranged from 0.001 to 0.09 nmol N L-1 h(-1). DON release represented approximate to 14% and approximate to 23% of \u3e10 mu m and (assimilation into biomass plus DON release), respectively. This implies that by overlooking DON release, N-2 fixation rates are underestimated. Net N-2 fixation rates were higher in the east and decreased significantly toward the west (r(s)=-0.487, p=0.002, and r(s)=-0.496, p=0.001, for the \u3e10 mu m and fractions, respectively). The sum of both fractions correlated with aerosol optical depth at 550 nm (AOD 550 nm) (r(s)=0.382, p=0.017) and phosphate (PO43-) concentrations (r(s)=0.453, p=0.018), suggesting an enhancement of diazotrophy as a response to aerosol inputs and phosphorus availability. In contrast, DON release was constant among size fractions and did not correlate with any of these variables. We also compared N-2 fixation rates obtained using the N-15(2) dissolved and bubble methods. The first gave average rates 50% (49% 39) higher than the latter, which supports the finding that previously published N-2 fixation rates are likely underestimated. We suggest that by combining N-2 fixation and DON release measurements using dissolved N-15(2), global N-2 fixation rates could increase enough to balance oceanic fixed nitrogen budget disequilibria

On the seasonal variability of the Canary Current and the Atlantic Meridional Overturning Circulation

Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 122 (2017): 4518–4538, doi:10.1002/2017JC012774.The Atlantic Meridional Overturning Circulation (AMOC) is continually monitored along 26°N by the RAPID-MOCHA array. Measurements from this array show a 6.7 Sv seasonal cycle for the AMOC, with a 5.9 Sv contribution from the upper mid-ocean. Recent studies argue that the dynamics of the eastern Atlantic is the main driver for this seasonal cycle; specifically, Rossby waves excited south of the Canary Islands. Using inverse modeling, hydrographic, mooring, and altimetry data, we describe the seasonal cycle of the ocean mass transport around the Canary Islands and at the eastern boundary, under the influence of the African slope, where eastern component of the RAPID-MOCHA array is situated. We find a seasonal cycle of −4.1 ± 0.5 Sv for the oceanic region of the Canary Current, and +3.7 ± 0.4 Sv at the eastern boundary. This seasonal cycle along the eastern boundary is in agreement with the seasonal cycle of the AMOC that requires the lowest contribution to the transport in the upper mid-ocean to occur in fall. However, we demonstrate that the linear Rossby wave model used previously to explain the seasonal cycle of the AMOC is not robust, since it is extremely sensitive to the choice of the zonal range of the wind stress curl and produces the same results with a Rossby wave speed of zero. We demonstrate that the seasonal cycle of the eastern boundary is due to the recirculation of the Canary Current and to the seasonal cycle of the poleward flow that characterizes the eastern boundaries of the oceans.RAPROCAN Project ; Instituto Español de Oceanografía; and as part of the SeVaCan project Grant Number: CTM2013-48695; Ministerio de Economía y Competividad; Apoyo al Personal Investigador en Formación2017-12-0

The Atlantic Water boundary current north of Svalbard in late summer

Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 122 (2017): 2269–2290, doi:10.1002/2016JC012486.Data from a shipboard hydrographic/velocity survey carried out in September 2013 of the region north of Svalbard in the Nansen Basin are analyzed to characterize the Atlantic Water (AW) boundary current as it flows eastward along the continental slope. Eight meridional transects across the current, spanning an alongstream distance of 180 km, allow for a detailed description of the current and the regional water masses. During the survey the winds were light and there was no pack-ice. The mean section reveals that the boundary current was O(40 km) wide, surface-intensified, with a maximum velocity of 20 cm/s. Its mean transport during the survey was 3.11 ± 0.33 Sv, of which 2.31 ± 0.29 Sv was AW. This suggests that the two branches of AW entering the Arctic Ocean via Fram Strait—the Yermak Plateau branch and the Svalbard branch—have largely combined into a single current by 30°E. At this location the boundary current meanders with a systematic change in its kinematic structure during offshore excursions. A potential vorticity analysis indicates that the flow is baroclinically unstable, consistent with previous observations of AW anticyclones offshore of the current as well as the presence of a near-field cyclone in this data set. Our survey indicates that only a small portion of the boundary current is diverted into the Kvitøya Trough (0.17 ± 0.08 Sv) and that the AW temperature/salinity signal is quickly eroded within the trough.National Science Foundation Grant Number: ARC-12640982017-09-2

Transcription factors Sp1 and p73 control the expression of the proapoptotic protein NOXA in the response of testicular embryonal carcinoma cells to cisplatin

Testicular germ cell tumors (TGCTs) are highly responsive to and curable by cisplatin-based chemotherapy even in advanced stages. We have studied the molecular mechanisms involved in the induction of apoptosis in response to cisplatin, and found that proapoptotic Noxa is transcriptionally up-regulated following cisplatin exposure, even in the absence of p53, in NTERA2 cisplatin-sensitive cells but not in 1411HP-resistant cells. Blockade of Noxa reduced the apoptotic response of embryonal carcinoma (EC) NTERA2 cells to cisplatin. A detailed analysis of the Noxa promoter revealed that p73 and Sp1-like factors, Sp1 and KLF6, played key roles in the transcriptional control of this gene. Overexpression of TAp73 induced Noxa whereas the dominant negative isoform ΔNp73, reduced the levels of Noxa after cisplatin exposure in NTERA2 and 2102EP. Interestingly, down-regulation of Sp1 increased Noxa expression in response to cisplatin. However, blockade of KLF6 decreased cisplatin-induced up-regulation of Noxa in EC cell lines. In addition, tissue microarray analyses of TGCTs revealed that expression of Noxa correlates with good clinical prognosis in patients with embryonal carcinoma. Thus, our data show the transcriptional network that regulates Noxa in EC cells, which is key for their apoptotic response to cisplatin-based chemotherapy, and propose Noxa as a predictive factor of therapeutic response

Abnormalities on 1q and 7q are associated with poor outcome in sporadic Burkitt's lymphoma. A cytogenetic and comparative genomic hybridization study

Comparative genomic hybridization (CGH) studies have demonstrated a high incidence of chromosomal imbalances in non-Hodgkin's lymphoma. However, the information on the genomic imbalances in Burkitt's Lymphoma (BL) is scanty. Conventional cytogenetics was performed in 34 cases, and long-distance PCR for t(8;14) was performed in 18 cases. A total of 170 changes were present with a median of four changes per case (range 1-22). Gains of chromosomal material (143) were more frequent than amplifications (5) or losses (22). The most frequent aberrations were gains on chromosomes 12q (26%), Xq (22%), 22q (20%), 20q (17%) and 9q (15%). Losses predominantly involved chromosomes 13q (17%) and 4q (9%). High-level amplifications were present in the regions 1q23-31 (three cases), 6p12-p25 and 8p22-p23. Upon comparing BL vs Burkitt's cell leukemia (BCL), the latter had more changes (mean 4.3 +/- 2.2) than BL (mean 2.7 +/- 3.2). In addition, BCL cases showed more frequently gains on 8q, 9q, 14q, 20q, and 20q, 9q, 8q and 14q, as well as losses on 13q and 4q. Concerning outcome, the presence of abnormalities on 1q (ascertained either by cytogenetics or by CGH), and imbalances on 7q (P=0.01) were associated with a short survival

FISH analysis of hematological neoplasias with 1p36 rearrangements allows the definition of a cluster of 2.5 Mb included in the minimal region deleted in 1p36 deletion syndrome

Rearrangements in the distal region of the short arm of chromosome 1 are recurrent aberrations in a broad spectrum of human neoplasias. However, neither the location of the breakpoints (BP) on 1p36 nor the candidate genes have been fully determined. We have characterized, by fluorescence in situ hybridization (FISH), the BP in 26 patients with hematological neoplasias and 1p36 rearrangements in the G-banding karyotype. FISH allowed a better characterization of all samples analyzed. Nine cases (35%) showed reciprocal translocations, 15 (58%) unbalanced rearrangements, and two (7%) deletions. We describe two new recurrent aberrations. In 18 of the 26 cases analyzed the BP were located in band 1p36, which is 25.5 Mb long. In 14 of these 18 cases (78%) and without distinction between myeloid and lymphoid neoplasias, the BP clustered in a 2.5 Mb region located between 1p36.32 and the telomere. Interestingly, this region is contained in the 10.5 Mb cluster on 1p36.22-1pter defined in cases with 1p36 deletion syndrome. The 2.5 Mb region, located on 1p36.32-1pter, has a higher frequency of occurrence of tandem repeats and segmental duplications larger than 1 kb, when compared with the 25.5 Mb of the complete 1p36 band. This could explain its proneness for involvement in chromosomal rearrangements in hematological neoplasias

Amino functionalized mesoporous silica nanoparticles encapsulated octahedral organoruthenium complex as an efficient platform for combatting cáncer.

In the process of synthesis of a new drug, as important as the drug itself is the formulation used, because the same compound can present a very different efficacy depending on how it is administered. In this work, we demonstrate how the antitumor capacity of a new octahedral organo-ruthenium complex, [Ru(ppy-CHO)(phen)2][PF6] is affected by its encapsulation in different types of mesoporous silica nanoparticles. The interactions between the Ru complex and the silica matrix and how these interactions are affected at two different pHs (7.4 and 5.4, mimicking physiological and endolysosomal acidic conditions, respectively) have been studied. The encapsulation has also been shown to affect the induction of apoptosis and necrosis and progression of the cell cycle compared to the free drug. The encapsulation of the Ru complex in nanoparticles functionalized with amino groups produced very high anticancer activity in cancer cells in vitro, especially against U87 glioblastoma cells, favoring cellular internalization and significantly increasing the anticancer capacity of the initial non-encapsulated Ru complex

Urinary exosome miR-146a is a potential marker of albuminuria in essential hypertension

BACKGROUND: There is increasing interest in using extracellular vesicle-derived microRNAs (miRNAs) as biomarkers in renal dysfunction and injury. Preliminary evidence indicates that miRNAs regulate the progression of glomerular disease. Indeed, exosomes from the renal system have provided novel evidence in the clinical setting of albuminuria. Thus, the aim of this study was to quantify the urinary miRNAs present in exosome and microvesicles (MVs), and to assess their association with the presence of increased urinary albumin excretion in essential hypertension. METHODS: Exosomes were collected from urine specimens from a cohort of hypertensive patients with (n = 24) or without albuminuria (n = 28), and from 20 healthy volunteers as a control group. Urinary exosomes were phenotyped by Western blot, tunable resistive pulse sensing, and electronic microscopy. Expression of miR-146a and miR-335* was analysed by qRT-PCR and any associations between albuminuria and exosomal miRNAs were analysed. RESULTS: Urinary miRNAs are highly enriched in exosome subpopulations compared to MVs, both in patients with or without increased albuminuria (p < 0.001), but not in the control group. High albuminuria was associated with 2.5-fold less miR-146a in exosomes (p = 0.017), whereas miR-146a levels in MV did not change. In addition, exosome miR-146a levels were inversely associated with albuminuria (r = 0.65, p < 0.0001), and discriminated the presence of urinary albumin excretion presence [area under the curve = 0.80, 95% confidence interval: 0.66-0.95; p = 0.0013]. CONCLUSIONS: Our results indicate that miRNAs were enriched in the urinary exosome subpopulation in hypertensive patients and that low miR-146a expression in exosomes was associated with the presence of albuminuria. Thus, urinary exosome miR-146a may be a potentially useful tool for studying early renal injury in hypertension