Infrared spectra in polarized light of crystalline chloroform

High resolution spectra in polarized light of oriented crystalline films of chloroform at 77°K were obtained. The ratio of intensities of Davydov components for different angles of polarization were also determined. Comparison with the oriented gas model approximation allowed the assignment of components to the proper representations of the D 2h factor group : all bands were assigned to the infrared active factor group symmetry species B 1u, B 2u, and B 3u corresponding to vibrations along a, b, and c crystallographic axes. Comparison with results of measurements in solid solutions in argon made by King allowed the distinction between crystallographic effect and isotopic shifts.Fil:Pomposiello, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina

Combination of DROOL rules and Protégé knowledge bases in the ONTO-H annotation tool

ONTO-H is a semi-automatic collaborative tool for the semantic annotation of documents, built as a Protégé 3.0 tab plug-in. Among its multiple functionalities aimed at easing the document annotation process, ONTO-H uses a rule-based system to create cascading annotations out from a single drag and drop operation from a part of a document into an already existing concept or instance of the domain ontology being used for annotation. It also gives support to the detection of name conflicts and instance duplications in the creation of the annotations. The rule system runs on top of the open source rule engine DROOLS and is connected to the domain ontology used for annotation by means of an ad-hoc programmed Java proxy

The Winckelmann300 Project: Dissemination of Culture with Virtual Reality at the Capitoline Museum in Rome

The best way to disseminate culture is, nowadays, the creation of scenarios with virtual and augmented reality that supply the visitors of museums with a powerful, interactive tool that allows to learn sometimes difficult concepts in an easy, entertaining way. 3D models derived from reality-based techniques are nowadays used to preserve, document and restore historical artefacts. These digital contents are also powerful instrument to interactively communicate their significance to non-specialist, making easier to understand concepts sometimes complicated or not clear. Virtual and Augmented Reality are surely a valid tool to interact with 3D models and a fundamental help in making culture more accessible to the wide public. These technologies can help the museum curators to adapt the cultural proposal and the information about the artefacts based on the different type of visitor’s categories. These technologies allow visitors to travel through space and time and have a great educative function permitting to explain in an easy and attractive way information and concepts that could prove to be complicated. The aim of this paper is to create a virtual scenario and an augmented reality app to recreate specific spaces in the Capitoline Museum in Rome as they were during Winckelmann’s time, placing specific statues in their original position in the 18th century

Traces Of Human Functional Activity: Moment-To-Moment Fluctuations In Fmri Data

Dynamic functional connectivity (dFC) measured by functional magnetic resonance imaging (fMRI) shows evidence of large-scale networks with highly dynamic (re) configurations. We propose a novel approach to extract traces of human brain function by the construction of a trajectory in a meaningful low-dimensional space. This allows studying dFC in more detail and identify possible meaningful brain states from the moment-to-moment fluctuations of the brain signals during resting state or naturalistic conditions such as passive movie watching. Specifically, we explored dynamic organization of sub-networks derived from the time-dependent graph Laplacian in combination with Riemannian manifold distance to measure dissimilarity over time of dFC and to subsequently build the trajectory of brain activity. As a proof-of-principle, we show results for an fMRI dataset containing both rest and movie epochs in 15 healthy participants. The movie condition varied (i.e., fearful, joyful, and neutral movie excerpts) and clearly influenced the subsequent resting-state period in terms of FC brain state

Pretransplantation 18F-Fluorodeoxyglucose positron emission tomography scan predicts outcome in patients with recurrent Hodgkin lymphoma or aggressive non-hodgkin lymphoma undergoing reduced-intensity conditioning follone by allogeneic stem cell transplantation

Background: The use of positron emission tomography (PET) scanning in Hodgkin lymphoma (HL) and aggressive non-Hodgkin lymphoma (HG-NHL) has recognized prognostic value in patients who are receiving chemotherapy or undergoing autologous stem cell transplantation (SCT). In contrast, the role of PET before reduced-intensity conditioning (RIC) and followed by allogeneic SCT has not been investigated to date. Methods: PET was used to assess 80 patients who had chemosensitive disease (34 patients with HG-NHL and 46 patients with HL) before they underwent allogeneic SCT: 42 patients had negative PET studies, and 38 patients had positive PET studies. Patients underwent allograft from matched related siblings (n = 41) or alternative donors (n = 39). Results: At the time of the last follow-up, 48 patients were alive (60%), and 32 had died. The 3-year cumulative incidence of nonrecurrence mortality and disease recurrence was 17% and 40%, respectively. The cumulative incidence of disease recurrence was significantly lower in the PET-negative patients (25% vs 56%; P =.007), but there was no significant difference between the patients with or without chronic graft-versus-host disease (P =.400). The patients who had negative PET studies before undergoing allogenic SCT also had significantly better outcomes in terms of 3-year overall survival (76% vs 33%; P =.001) and 3-year progression-free survival (73% vs 31%; P =.001). On multivariate analysis, overall survival was influenced by PET status (hazard ratio [HR], 3.35), performance status (HR, 5.15), and type of donor (HR, 6.26 for haploidentical vs sibling; HR, 1.94 for matched unrelated donor vs sibling). Conclusions: The current results indicated that PET scanning appears to be an accurate tool for assessing prognosis in patients who are eligible for RIC allografting

Neuroimaging Evidence of Major Morpho-Anatomical and Functional Abnormalities in the BTBR T+TF/J Mouse Model of Autism

BTBR T+tf/J (BTBR) mice display prominent behavioural deficits analogous to the defining symptoms of autism, a feature that has prompted a widespread use of the model in preclinical autism research. Because neuro-behavioural traits are described with respect to reference populations, multiple investigators have examined and described the behaviour of BTBR mice against that exhibited by C57BL/6J (B6), a mouse line characterised by high sociability and low self-grooming. In an attempt to probe the translational relevance of this comparison for autism research, we used Magnetic Resonance Imaging (MRI) to map in both strain multiple morpho-anatomical and functional neuroimaging readouts that have been extensively used in patient populations. Diffusion tensor tractography confirmed previous reports of callosal agenesis and lack of hippocampal commissure in BTBR mice, and revealed a concomitant rostro-caudal reorganisation of major cortical white matter bundles. Intact inter-hemispheric tracts were found in the anterior commissure, ventro-medial thalamus, and in a strain-specific white matter formation located above the third ventricle. BTBR also exhibited decreased fronto-cortical, occipital and thalamic gray matter volume and widespread reductions in cortical thickness with respect to control B6 mice. Foci of increased gray matter volume and thickness were observed in the medial prefrontal and insular cortex. Mapping of resting-state brain activity using cerebral blood volume weighted fMRI revealed reduced cortico-thalamic function together with foci of increased activity in the hypothalamus and dorsal hippocampus of BTBR mice. Collectively, our results show pronounced functional and structural abnormalities in the brain of BTBR mice with respect to control B6 mice. The large and widespread white and gray matter abnormalities observed do not appear to be representative of the neuroanatomical alterations typically observed in autistic patients. The presence of reduced fronto-cortical metabolism is of potential translational relevance, as this feature recapitulates previously-reported clinical observations

Presencia de enfermedades en majadas caprinas de las quebradas áridas de Jujuy y Salta

Una encuesta transversal ad hoc se llevó a cabo en 40 unidades productoras familiares (UPF) de las áridas en las provincias de Jujuy y Salta, con el objetivo de describir la presencia de enfermedades en sus majadas caprinas. Se recabaron datos del manejo general y sanitario en particular. Se procesaron sueros para el diagnóstico de brucelosis por las pruebas tamiz del antígeno bufferado en placa (BPA) y confirmadas por polarización fluorecente (FPA), para paratuberculosis (PTBC), artritis-encefalitis (CAEV), chlamydiosis, herpesvirus caprino tipo 1 (CaHV-1), fiebre Q y toxoplasmosis se utilizó ELISA indirecto y para leptospirosis microaglutinación. Se determinaron los valores de Cu2+, Zn2+, Mg2+ y Ca2+ sérico por espectofotómetría de absorción atómica. En heces, se realizó el recuento de huevos de helmintos por gramo (hpg) y la diferenciación de géneros por coprocultivo.Las UPF tuvieron en promedio 91,4±75 caprinos (sin contar cabritos), siendo el 80,9% cabras madres. La cría fue extensiva con encierre nocturno y la alimentación de la majada se basó mayormente en el pastoreo de los cerros y de cultivos y rastrojos. La parición se concentró en dos períodos, uno principal en abril-julio y una segunda parición de menor número de cabras ubicada en octubre-diciembre. El 92,3% de los productores desparasitaba en forma rutinaria toda la majada con ivermectina, albendazole o closantel y el 71,8 % trataba los piojos. En un 61,5% de las UPF se inyectaba mineralizantes-vitamínicos a toda la majada y en solo un 15,4% se vacunaba contra enfermedades clostridiales. El promedio de la tasa de mortalidad anual por majada fue del 14,2±11%. En el 86,4% de las UPF se registró la ocurrencia de abortos, y de estos el 42,9% presentó retención de placenta. El promedio intramajada de abortos comunicados fue del 8,6±8,5%. El 75% de las UPF declaró tener ectima contagioso, aunque solo el 22% dijo que era grave. Se registraron un 30,4% cuadros de muertes agudas probablemente por infecciones clostridiales. En el 72,7% de las UPF se registraron problemas de mastitis. El porcentaje de UPF con seroprevalencia positiva a brucelosis, leptospirosis, paratuberculosis, toxoplasmosis, clamidiosis, CpHV-1, CAEV y fiebre Q fue respectivamente de 0%, 25%, 50%, 42,8%, 88,8%, 85,7%, 12,5% y 0%. Las UPF destacaron la importancia de los piojos (Linognathus spp.: 87%). Los promedios de los hpg fueron en abril-mayo, julio-agosto y septiembre-noviembre respectivamente de 407, 332 y 59 con Haemonchus y en menor medida Trichostrongylus como nematodes predominantes. En el 76% de las UPF se diagnosticó Fasciolosis. El 30,8% de las UPF describió la presencia a la faena de quistes hidatídicos. En un 31% de las majadas se describió bocio. Los promedios por UPF de cobre, zinc, magnesio y calcio séricos fueron respectivamente 0,48 ±0,10 ppm, 0,73±0,16 ppm, 2,4±0,47mg/d y 7,25±2,25 mg/dl. Se describieron casos de timpanismo espumoso (43,5%), muertes (36%) atribuidas por los propietarios a la ingesta de ñusco, mio mio y sunchillo y un 38,1% de las UPF dijeron padecer casos de intoxicación con cegadera (Heterophyllaea pustulata). Un 66,7% de los propietarios declararon tener problemas de pérdidas de animales en el monte a causa mayormente de pumas. Estos primeros resultados en cuanto a mortandad y presencia de enfermedades y zoonosis, muestran la importancia de llevar a cabo estudios más específicos respecto de la salud de las majadas para incrementar la producción y el bienestar general de las familias productoras.Fil: Suarez, V. H.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Dodero, Ana Mariela. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nievas, J. D.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Martinez, G. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Bertoni, E. A.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Salatin, A. O.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Viñabal, A. E.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Grossberger, G.. No especifíca;Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pinto, G.. Instituto Nacional de Tecnología Agropecuaria; Argentin

Disease presence in goat flocks on temperate valleys of Argentina’s Northwest

Una encuesta transversal ad hoc se llevó a cabo en veintitrés majadas caprinas correspondientes a unidades productivas (UP) familiares extensivos (UPF, n=19) y semiintensivas (UPL, n=4) dedicadas a la producción de leche en los valles templados de Salta y Jujuy con el objetivo de describir la presencia de enfermedades en sus majadas. Se recabaron datos del manejo general y sanitario en particular. Se procesaron sueros para el diagnóstico de brucelosis por las pruebas tamiz del antígeno bufferado en placa y confirmadas por polarización fluorescente. Paraparatuberculosis, herpesvirus caprino tipo 1 (CaHV-1), neosporosis y toxoplasmosis se utilizó ELISA indirecto y para leptospirosis microaglutinación. Se determinaron los valores de Cu2+, Zn2+, Mg2+ y Ca2+ sérico por espectrofotometría de absorción atómica. En heces, se realizó el recuento de huevos de helmintos por gramo (hpg) y la diferenciación de géneros por coprocultivo. Las UP muestreadas crían en promedio 48,4 ±30,7 (UPF) y 221,3 ±78,7 (UPL) caprinos respectivamente, sin contar los cabritos menores a los 45 días de edad. La cría de las UPF fue extensiva con encierre nocturno y la alimentación sobre pastizal natural mientras que las UPL combinaban pasturas bajo riego con suplementación en base de concentrados. Existen dos períodos de pariciones al año; el principal donde pare casi la totalidad de la majada se localiza desde fines de marzo a fines de septiembre y el segundo período donde pare una menor proporción de cabras se ubica desde septiembre a diciembre. El 94,7% de los productores desparasitaba en forma rutinaria toda la majada y el 75% trataban los piojos. En un 41,2% de las UP se inyectaba mineralizantes-vitamínicos a toda la majada y en solo un 15% vacunaba contra enfermedades Clostridiales. El promedio de la tasa de mortalidad anual fue del 20,1±9,8% y 7,8±3,0% respectivamente en UPF y UPL. En el 100% de las UPF se registró la ocurrencia de abortos, y de estos el 25% presentó retención de placenta. El promedio intramajada de abortos comunicados fue del 14,5 ±11,6%. El 58,8% de las UP declaró tener casos de ectima contagioso. Se registraron un 26,7% cuadros de muertes agudas probablemente por infecciones Clostridiales. En el 93,3% de las UPF se registraron problemas de mastitis. El porcentaje de UP con seroprevalencia positiva a brucelosis, leptospirosis, paratuberculosis, toxoplasmosis, neosporosis y CpHV-1 fue respectivamente de 0%, 0%, 0%, 73,3%, 40% y 87,5%. Los promedios de los hpg de invierno – primavera y los de verano-otoño fueron respectivamente de 965 y 2859 hpg, con Haemonchus y Trichostrongylus y en menor medida Teladorsagia como nematodes predominantes. En el 31,6% de las UP se diagnosticó Fasciolosis. El 66,7% de las UP describió la presencia, a la faena, de quistes hidatídicos. En un 58% de las majadas se describió bocio. Los promedios por UP de cobre, zinc, magnesio y calcio séricos fueron respectivamente 0,59±0,10 ppm, 0,67±0,05 ppm, 2,30±0,29mg/dl y 8,42±1,92 mg/dl. Sin embargo, para el cobre y el calcio los valores promedios difieren (p<0,05) entre las UPF (0,66±0,08 ppm y 9,36 ±1,85 mg/dl) y las UPL (0,54±0,06 ppm y 7,02±0,76 mg/dl). Un 63,6% de las UP dijo tener intoxicación con cegadera (Heterophyllaea pustulata) y de muertes (50%) en animales jóvenes atribuidas al romerillo (Baccharis coridifolia) y en menor medida a la hediondilla (Cestrum parqui). Un 58,8% de los propietarios declararon tener problemas de pérdidas de animales principalmente por pumas. Estos primeros resultados muestran la importancia de llevar a cabo estudios más específicos respecto de la salud de las majadas para incrementar la producción y el bienestar general de las familias productoras.A cross-sectional survey was performed in 23 goat flocks (UP) of family extensive farming units (UPF) and semi-intensive farming units (UPL) from the temperate valleys of Jujuy and Salta provinces, with the aim of describing disease occurrence in goat flocks. Farmers were subjected to an ad hoc survey to gather data on general management and specific health issues. Sera were processed to diagnose brucellosis using the BPA screening test and positive sera were confirmed by FPA test. Paratuberculosis, caprine herpesvirus 1, toxoplasmosis and neosporosis were tested by indirect ELISA, and leptospirosis by microagglutination test. Serum Cu2+, Zn2+, Mg2+ and Ca2+ were determined by atomic absorption spectrophotometer. Fecal samples were taken for eggs counts per gram of feaces (epg) and identifying genera by coproculture. UP had on average 48,4 ±30,7 (UPF) y 221,3 ±78,7 (UPL) goats (not including kids). UPF animals are extensively bred, with nocturnal enclosure, and flock feeding is based on grazing the hills and UPL combined pastures under irrigation with concentrate-based supplementation. Kidding is concentrated in two periods: from late March to late September, when almost all the flock kids, and a second minor kid period that was from September to December. Farmers (94,7%) routinely treat all the flock against parasites and the 71,8% treat against lice. The 41,2% of UP provide all the flock with mineral-vitamin mixtures and only the 15, % vaccinated against clostridium diseases. Mean annual mortality rate per flock was 20,1±9,8% and 7,8±3,0% respectively for UPF and UPL. Abortions were recorded in 100% of the UP, 25% with retained placenta. Mean within-flock reported abortions were 14,5 ±11,6%. Contagious echtyma (orf) was detected in 58,8% of the UP and acute deaths probably due to clostridial infections were recorded in 26,7% of the UP. Mastitis-associated problems were recorded in 93,3% of UP. Positive seroprevalence of brucellosis, leptospirosis, paratuberculosis, toxoplasmosis, neosporosis and CpHV-1, in UP was 0%, 25%, 50%, 42,8%, 88,8%, 85,7%, 12,5% and 0% respectively. Mean epg were 965 and 2859 for winter-spring and summer-autumn respectively, with Haemonchus and Trichostrongylus and secondly Teladorsagia being the prevailing nematodes. Fasciola infections were recorded in 31,6% of the UP. The presence of hydatid cysts in slaughtered animals were indicated by 66,7% of UP. Signs of goiter were described in 58% of the flocks. Mean serum cooper, zinc, magnesium and calcium per UP were 0,59±0,10 ppm, 0,67±0,05 ppm, 2,30±0,29mg/dl and 8,42±1,92 mg/dl respectively. However cooper and calcium was different (p<0,05) between UPF (0,66±0,08 ppm and 9,36±1,85 mg/dl) and UPL (0,54±0,06 ppm and 7,02±0,76 mg/dl). A 63,6% of the UP reported cases of intoxication by cegadera (Heterophyllaea pustulata) and death (50%) were reported and attributed by farmers to the ingestion of romerillo (Baccharis coridifolia) and secondly of hediondilla (Cestrum parqui) by young animals. Some farmers (58.8%) indicated loss of animals mostly due to the attack by pumas. These first results regarding show the importance of conducting specific studies about the health of flocks in order to increase competitiveness in goat production and the welfare of farming families.Fil: Suarez, Víctor Humberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Dodero, Ana Mariela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Almudevar, F. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Bertoni, E. A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Salatin, A. O.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Viñabal, A. E.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Saldaño, R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Martínez, G. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Fiorentino, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; ArgentinaFil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; ArgentinaFil: Romera, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentin

Axicabtagene ciloleucel treatment is more effective in primary mediastinal large B-cell lymphomas than in diffuse large B-cell lymphomas: the Italian CART-SIE study

Axicabtagene ciloleucel showed efficacy for relapsed/refractory large B-cell lymphomas (LBCL), including primary mediastinal B-cell lymphomas (PMBCL); however, only few PMBCLs were reported. Aim was to evaluate efficacy and safety of axicabtagene ciloleucel in patients with PMBCL compared to those with other LBCL, enrolled in the Italian prospective observational CART-SIE study. PMBCLs (n = 70) were younger, with higher percentage of bulky and refractory disease, compared to other LBCLs (n = 190). Median follow-up time for infused patients was 12.17 months (IQR 5.53,22.73). The overall (complete + partial) response rate (ORR,CR + PR) after bridging was 41% for PMBCL and 28% for other LBCL, p = 0.0102. Thirty days ORR was 78% (53/68) with 50% (34) CR in PMBCL, and 75% (141/187) with 53% (100) CR in other LBCL, p = 0.5457. Ninety days ORR was 69% (45/65) with 65% (42) CR in PMBCL, and 54% (87/162) with 47% (76) CR in other LBCL; progressive disease was 21% in PMBCL and 45% in other LBCL, p = 0.0336. Twelve months progression-free survival was 62% (95% CI: 51–75) in PMBCL versus 48% (95% CI: 41–57) in other LBCL, p = 0.0386. Twelve months overall survival was 86% (95% CI: 78–95) in PMBCL versus 71% (95% CI: 64–79) in other LBCL, p = 0.0034. All grade cytokine release syndrome was 88% (228/260); all grade neurotoxicity was 34% (88/260), with 6% of fatal events in PMBCL. Non-relapse mortality was 3%. In conclusion, PMBCLs achieved significantly better response and survival rates than other LBCLs