Accuracy of at-sea commercial size grading of tiger prawns (Penaeus esculentus and P-semisulcatus) in the Australian northern prawn fishery

The size-frequency distribution of the commercial catch is often used as the basis of fisheries stock assessments (Paul and Morgan, 1987; Gulland and Rosenberg, 1992) because most dynamic processes of populations (growth, survival, recruitment) are reflected in changes in this distribution. The data are generally collected, often at great expense, by sampling the catch at landing sites and markets, or onboard fishing vessels. Size-frequency distributions of prawns (Penaeus esculentus and P. semisulcatus) can also be obtained from fish processors, who grade landings by size. These data are easier and cheaper to obtain than research samples, but unfortunately they are also considered less accurate and lack spatial information. However, they have been used in stock assessment of prawns in Kuwait (Jones and van Zalinge, 1981) and Malaysia (Simpson and Kong, 1978). It is often difficult to relate size data obtained from a processor to time and place of capture of the prawns, but this is not the case when the product is packed onboard, as in Australia's northern prawn fishery (NPF). Trawler operators in the NPF have voluntarily recorded size composition since 1985, when provision for this was made in operators' daily logbooks (between 30% and 45% of the tiger prawn catch reported in the logbooks contain size information). These books are therefore the most comprehensive source of information on the spatial and temporal size distribution of the commercial catch of the NPF. Present assessments of the fishery are based on deterministic growth and deterministic seasonal recruitment patterns (Wang and Die, 1996) and do not use size-structured data. If available, these data would help relax the recruitment and improve current stock assessments of the NPF. Before the size data recorded in the logbooks can be used, however, the accuracy of size grading at sea needs to the assessed. This paper examines the accuracy of grading tiger prawns, by using data collected from a private firm, A. Raptis and Sons, that operates a large modern processing factory that regularly assesses the onboard grading of product purchased from NPF trawler operators. Although the work presented here relates specifically to the NPF, the practice of onboard size grading is widespread in other fisheries around the world. Therefore our methods have potential application to other fisheries

Effects of Hypericum perforatum with Vitex agnus-catus in the treatment of menopausal symptoms

Background: Interest in alternatives to hormone therapy (HT) for menopausal symptoms increased following its association with serious health risks. In terms of phytotherapeutic interventions, while traditional use supports a range of herbs for treating menopausal symptoms, evidence from rigorous scientific trials is limited, and has largely focused on the phytoestrogenic plants. Because of some safety concerns over long-term use of isoflavones, the present study focused on two non-estrogenic herbs, Hypericum perforatum and Vitex agnus-castus, also employed in this context in the Anglo-American and European traditions. Both herbs have shown effectiveness for the alleviation of symptoms of premenstrual syndrome (PMS), which is reported to be more severe during the perimenopause, and may account for many of the so-called 'menopausal symptoms' at this time. Research on menopausal vasomotor symptoms is prone to substantial placebo responses. There has been much interest in increasing our understanding of the placebo response with a view to controlling it in clinical research and harnessing it in clinical practice. Methods & Results: A double-blind, randomised controlled trial (RCT), with a 16-week treatment phase, was conducted on 100 late-perimenopause and early postmenopause women. The herbal combination (Hypericum and Vitex) was not found to be superior to placebo for any of the endpoints - daily weighted flushing scores, overall menopausal symptoms (on the Greene Climacteric Scale) and depression (on the Hamilton Depression Inventory). However, significant improvements across the treatment phase were observed in both arms for all of these outcome measures. No significant change was found for either group on the Utian quality of life scale. The effects of the herbal combination were also examined on PMS-like symptoms in the small sub-population of late-perimenopausal women, and found to be superior to placebo for total PMS-like symptoms and the sub-clusters, PMS-D (depression) and PMS-C (cravings). The active treatment group also showed significant improvements on PMS-A (anxiety) and PMS-H (hydration), although these effects were not superior to placebo. Predictors of the placebo response were investigated and found to include study-entry anxiety for the outcome measures of flushing, depression and overall menopausal symptoms, and improvement during non-treatment run-in for depression and overall symptoms. Because no difference had been found between 'active' and placebo groups in the menopause RCT, it was hypothesised that the same predictors would predict the response to the study treatment. However, low anxiety was significantly associated with improvement in this group. None of the other variables that predicted the placebo response was relevant to the study treatment response. This finding is discussed with reference to the possibility that 'drug' effects and placebo effects are not necessarily additive, and that the same magnitude of effect in both arms might not necessarily imply activity via the same pathways. Conclusions: This research contributes to the growing body of scientific knowledge about evidence-based complementary therapies that informs the community, health-care providers and regulatory authorities. The findings may facilitate identification of potential placebo responders in future research. The need for more research in the area of mechanisms of placebo versus active responses is supported

Toroidal automorphic forms, Waldspurger periods and double Dirichlet series

The space of toroidal automorphic forms was introduced by Zagier in the 1970s: a GL_2-automorphic form is toroidal if it has vanishing constant Fourier coefficients along all embedded non-split tori. The interest in this space stems (amongst others) from the fact that an Eisenstein series of weight s is toroidal for a given torus precisely if s is a non-trivial zero of the zeta function of the quadratic field corresponding to the torus. In this paper, we study the structure of the space of toroidal automorphic forms for an arbitrary number field F. We prove that it decomposes into a space spanned by all derivatives up to order n-1 of an Eisenstein series of weight s and class group character omega precisely if s is a zero of order n of the L-series corresponding to omega at s, and a space consisting of exactly those cusp forms the central value of whose L-series is zero. The proofs are based on an identity of Hecke for toroidal integrals of Eisenstein series and a result of Waldspurger about toroidal integrals of cusp forms combined with non-vanishing results for twists of L-series proven by the method of double Dirichlet series.Comment: 14 page

Excavaciones en Heracleópolis Magna (Egipto)

The excavation in Herakleópolis Magna is a project belonging to the National Archaeological Museum. The works have been financed by the Ministry of Culture. Egyptian authorities, trough the Supreme Council of Antiquities, have supported the project, always granting their co-operation, thus making it feasible. The main objective of the project is to study the history of Heracleópolis Magna, trying to obtain direct information that would allow a better knowledge of the history of the town and its territory. Until 2004 the works has been focused on the Herishef Temple, Third Intermediate Period Necropolis and First Intermediate Period Necropolis.La excavación de Heracleópolis Magna es un proyecto del Museo Arqueológico Nacional. Los trabajos han sido auspiciados y financiados por el Ministerio de Cultura. Las autoridades egipcias, a través del Supreme Council of Antiquities han apoyado el proyecto, concediendo todas las facilidades para su realización. El objetivo concreto es estudiar y divulgar la historia de Heracleópolis Magna, tratando de obtener información directa que permita un mejor conocimiento de la historia de la ciudad y de su territorio. Hasta el año 2004 se han realizado 20 campañas de excavación en el Templo del dios local Herishef, en la necrópolis del Primer Periodo Intermedio, momento en el que Heracleópolis era la capital de Egipto, y en el cementerio del Tercer Periodo Intermedio

Muscle-Type Nicotinic Receptor Blockade by Diethylamine, the Hydrophilic Moiety of Lidocaine

Lidocaine bears in its structure both an aromatic ring and a terminal amine, which can be protonated at physiological pH, linked by an amide group. Since lidocaine causes multiple inhibitory actions on nicotinic acetylcholine receptors (nAChRs), this work was aimed to determine the inhibitory effects of diethylamine (DEA), a small molecule resembling the hydrophilic moiety of lidocaine, on Torpedo marmorata nAChRs microtransplanted to Xenopus oocytes. Similarly to lidocaine, DEA reversibly blocked acetylcholine-elicited currents (IACh) in a dose-dependent manner (IC50 close to 70 μM), but unlike lidocaine, DEA did not affect IACh desensitization. IACh inhibition by DEA was more pronounced at negative potentials, suggesting an open-channel blockade of nAChRs, although roughly 30% inhibition persisted at positive potentials, indicating additional binding sites outside the pore. DEA block of nAChRs in the resting state (closed channel) was confirmed by the enhanced IACh inhibition when pre-applying DEA before its co-application with ACh, as compared with solely DEA and ACh co-application. Virtual docking assays provide a plausible explanation to the experimental observations in terms of the involvement of different sets of drug binding sites. So, at the nAChR transmembrane (TM) domain, DEA and lidocaine shared binding sites within the channel pore, giving support to their open-channel blockade; besides, lidocaine, but not DEA, interacted with residues at cavities among the M1, M2, M3, and M4 segments of each subunit and also at intersubunit crevices. At the extracellular (EC) domain, DEA and lidocaine binding sites were broadly distributed, which aids to explain the closed channel blockade observed. Interestingly, some DEA clusters were located at the α-γ interphase of the EC domain, in a cavity near the orthosteric binding site pocket; by contrast, lidocaine contacted with all α-subunit loops conforming the ACh binding site, both in α-γ and α-δ and interphases, likely because of its larger size. Together, these results indicate that DEA mimics some, but not all, inhibitory actions of lidocaine on nAChRs and that even this small polar molecule acts by different mechanisms on this receptor. The presented results contribute to a better understanding of the structural determinants of nAChR modulation.This work was supported by grants BFU2012-31359, BFU2012-39092-C02-01, BFU2011-25920, and CSD2008-00005 from the MINECO and PROMETEO/2014/11 from GVA (Spain)

Xenopus Oocytes as a Powerful Cellular Model to Study Foreign Fully-Processed Membrane Proteins

The use of Xenopus oocytes in electrophysiological and biophysical research constitutes a long and successful story, providing major advances to the knowledge of the function and modulation of membrane proteins, mostly receptors, ion channels, and transporters. Earlier reports showed that these cells are capable of correctly expressing heterologous proteins after injecting the corresponding mRNA or cDNA. More recently, the Xenopus oocyte has become an outstanding host–cell model to carry out detailed studies on the function of fully-processed foreign membrane proteins after their microtransplantation to the oocyte. This review focused on the latter overall process of transplanting foreign membrane proteins to the oocyte after injecting plasma membranes or purified and reconstituted proteins. This experimental approach allows for the study of both the function of mature proteins, with their native stoichiometry and post-translational modifications, and their putative modulation by surrounding lipids, mostly when the protein is purified and reconstituted in lipid matrices of defined composition. Remarkably, this methodology enables functional microtransplantation to the oocyte of membrane receptors, ion channels, and transporters from different sources including human post-mortem tissue banks. Despite the large progress achieved over the last decades on the structure, function, and modulation of neuroreceptors and ion channels in healthy and pathological tissues, many unanswered questions remain and, most likely, Xenopus oocytes will continue to help provide valuable responses.The work in the authors’ laboratories has been supported by grants SAF2017-82977-P (AEI/FEDER, UE) and PGC2018-093505-B-I00 from MINECO and GRE17-01 from Universidad de Alicante (Spain)

Application de la méthode LSPIV pour la mesure de champs de vitesse et de débits de crue sur modèle réduit et en rivière

International audienceLSPIV technique enables the measurement of surface flow velocities using image sequence analysis. EDF and Irstea partnership made possible the development of Fudaa‑LSPIV freeware by DeltaCAD Company. Two software applications at flume and field scales are detailed: (i) bed shear stresses were calculated owing to LSPIV velocities, water depth and bathymetry for a physical model of the Old Rhine; (ii) the software was used to optimize the calculation parameters of LSPIV flood discharge measurement stations in Mediterranean rivers.La technique LSPIV (Large Scale Particle Image Velocimetry) permet de mesurer les vitesses de surface d'un écoulement par analyse de séquence d'images. Pour faciliter l'application opérationnelle de la méthode, un logiciel, Fudaa-LSPIV, a été développé par la société DeltaCAD dans le cadre d'une collaboration entre EDF et Irstea. Deux applications en laboratoire et en rivière sont présentées : (i) couplée avec des mesures de hauteur d'eau et de bathymétries, la LSPIV a permis d'estimer des paramètres de Shields sur le modèle physique à fond mobile du Vieux-Rhin ; (ii) le logiciel a été utilisé pour procéder à des analyses de sensibilité pour paramétrer ainsi au mieux les stations LSPIV de mesure de débit en crue de rivières cévenoles

A Placebo‐Controlled Double‐Blinded Randomized Pilot Study of Combination Phytotherapy in Biochemically Recurrent Prostate Cancer

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136500/1/pros23317_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136500/2/pros23317.pd