855 research outputs found
The identification of mitochondrial DNA variants in glioblastoma multiforme
Background:
Mitochondrial DNA (mtDNA) encodes key proteins of the electron transfer chain (ETC), which produces ATP through oxidative phosphorylation (OXPHOS) and is essential for cells to perform specialised functions. Tumor-initiating cells use aerobic glycolysis, a combination of glycolysis and low levels of OXPHOS, to promote rapid cell proliferation and tumor growth. Glioblastoma multiforme (GBM) is an aggressively malignant brain tumor and mitochondria have been proposed to play a vital role in GBM tumorigenesis.
Results:
Using next generation sequencing and high resolution melt analysis, we identified a large number of mtDNA variants within coding and non-coding regions of GBM cell lines and predicted their disease-causing potential through in silico modeling. The frequency of variants was greatest in the D-loop and origin of light strand replication in non-coding regions. ND6 was the most susceptible coding gene to mutation whilst ND4 had the highest frequency of mutation. Both genes encode subunits of complex I of the ETC. These variants were not detected in unaffected brain samples and many have not been previously reported. Depletion of HSR-GBM1 cells to varying degrees of their mtDNA followed by transplantation into immunedeficient mice resulted in the repopulation of the same variants during tumorigenesis. Likewise, de novo variants identified in other GBM cell lines were also incorporated. Nevertheless, ND4 and ND6 were still the most affected genes. We confirmed the presence of these variants in high grade gliomas.
Conclusions:
These novel variants contribute to GBM by rendering the ETC. partially dysfunctional. This restricts metabolism to anaerobic glycolysis and promotes cell proliferation
The C-Band All-Sky Survey: Instrument design, status, and first-look data
The C-Band All-Sky Survey (C-BASS) aims to produce sensitive, all-sky maps of
diffuse Galactic emission at 5 GHz in total intensity and linear polarization.
These maps will be used (with other surveys) to separate the several
astrophysical components contributing to microwave emission, and in particular
will allow an accurate map of synchrotron emission to be produced for the
subtraction of foregrounds from measurements of the polarized Cosmic Microwave
Background. We describe the design of the analog instrument, the optics of our
6.1 m dish at the Owens Valley Radio Observatory, the status of observations,
and first-look data.Comment: 10 pages, 11 figures, published in Proceedings of SPIE MIllimeter,
Submillimeter, and Far-Infrared Detectors and Instrumentation for Astronomy V
(2010), Vol. 7741, 77411I-1 - 77411I-1
High speed solid rotor permanent magnet machines: concept and design
This paper proposes a novel solid rotor topology for an Interior Permanent Magnet (IPM) machine, adopted in this case for an aircraft starter-generator design. The key challenge in the design is to satisfy two operating conditions which are: a high torque at start and a high speed at cruise. Conventional IPM topologies which are highly capable of extended field weakening are found to be limited at high speed due to structural constraints associated with the rotor material. To adopt the IPM concept for high speed operation, it is proposed to adopt a rotor constructed from semi-magnetic stainless steel, which has a higher yield strength than laminated silicon steel. To maintain minimal stress levels and also minimize the resultant eddy current losses due to the lack of laminations, different approaches are considered and studied. Finally, to achieve a better tradeoff between the structural and electromagnetic constraints, a novel slitted approach is implemented on the rotor. The proposed rotor topology is verified using electromagnetic, static structural and dynamic structural Finite Element (FE) analyses. An experiment is performed to confirm the feasibility of the proposed rotor. It is shown that the proposed solid rotor concept for an IPM fulfils the design requirements whilst satisfying the structural, thermal and magnetic limitations
Prediction of a neuropeptidome for the eyestalk ganglia of the lobster Homarus americanus using a tissue-specific de novo assembled transcriptome
In silico transcriptome mining is a powerful tool for crustacean peptidome prediction. Using homology-based BLAST searches and a simple bioinformatics workflow, large peptidomes have recently been predicted for a variety of crustaceans, including the lobster, Homarus americanus. Interestingly, no in silico studies have been conducted on the eyestalk ganglia (lamina ganglionaris, medulla externa, medulla interna and medulla terminalis) of the lobster, although the eyestalk is the location of a major neuroendocrine complex, i.e., the X-organ-sinus gland system. Here, an H. americanus eyestalk ganglia-specific transcriptome was produced using the de novo assembler Trinity. This transcriptome was generated from 130,973,220 Illumina reads and consists of 147,542 unique contigs. Eighty-nine neuropeptide-encoding transcripts were identified from this dataset, allowing for the deduction of 62 distinct pre/preprohormones. Two hundred sixty-two neuropeptides were predicted from this set of precursors; the peptides include members of the adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin B, allatostatin C, bursicon α, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone (CHH), CHH precursor-related peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, elevenin, FMRFamide-like peptide, glycoprotein hormone α2, glycoprotein hormone β5, GSEFLamide, intocin, leucokinin, molt-inhibiting hormone, myosuppressin, neuroparsin, neuropeptide F, orcokinin, orcomyotropin, pigment dispersing hormone, proctolin, pyrokinin, red pigment concentrating hormone, RYamide, short neuropeptide F, SIFamide, sulfakinin, tachykinin-related peptide and trissin families. The predicted peptides expand the H. americanus eyestalk ganglia neuropeptidome approximately 7-fold, and include 78 peptides new to the lobster. The transcriptome and predicted neuropeptidome described here provide new resources for investigating peptidergic signaling within/from the lobster eyestalk ganglia
The Evolution of the Galaxy Stellar Mass Function at z= 4-8: A Steepening Low-mass-end Slope with Increasing Redshift
We present galaxy stellar mass functions (GSMFs) at 4-8 from a
rest-frame ultraviolet (UV) selected sample of 4500 galaxies, found via
photometric redshifts over an area of 280 arcmin in the CANDELS/GOODS
fields and the Hubble Ultra Deep Field. The deepest Spitzer/IRAC data
yet-to-date and the relatively large volume allow us to place a better
constraint at both the low- and high-mass ends of the GSMFs compared to
previous space-based studies from pre-CANDELS observations. Supplemented by a
stacking analysis, we find a linear correlation between the rest-frame UV
absolute magnitude at 1500 \AA\ () and logarithmic stellar mass
() that holds for galaxies with . We
use simulations to validate our method of measuring the slope of the - relation, finding that the bias is minimized with a hybrid
technique combining photometry of individual bright galaxies with stacked
photometry for faint galaxies. The resultant measured slopes do not
significantly evolve over 4-8, while the normalization of the trend
exhibits a weak evolution toward lower masses at higher redshift. We combine
the - distribution with observed rest-frame UV luminosity
functions at each redshift to derive the GSMFs, finding that the low-mass-end
slope becomes steeper with increasing redshift from
at to at
. The inferred stellar mass density, when integrated over
-, increases by a factor of
between and and is in good agreement with the time integral of the
cosmic star formation rate density.Comment: 27 pages, 17 figures, ApJ, in pres
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Algorithms for Olfactory Search across Species
Localizing the sources of stimuli is essential. Most organisms cannot eat, mate, or escape without knowing where the relevant stimuli originate. For many, if not most, animals, olfaction plays an essential role in search. While microorganismal chemotaxis is relatively well understood, in larger animals the algorithms and mechanisms of olfactory search remain mysterious. In this symposium, we will present recent advances in our understanding of olfactory search in flies and rodents. Despite their different sizes and behaviors, both species must solve similar problems, including meeting the challenges of turbulent airflow, sampling the environment to optimize olfactory information, and incorporating odor information into broader navigational systems
The mass evolution of the first galaxies: stellar mass functions and star formation rates at in the CANDELS GOODS-South field
We measure new estimates for the galaxy stellar mass function and star
formation rates for samples of galaxies at using data in
the CANDELS GOODS South field. The deep near-infrared observations allow us to
construct the stellar mass function at directly for the first time.
We estimate stellar masses for our sample by fitting the observed spectral
energy distributions with synthetic stellar populations, including nebular line
and continuum emission. The observed UV luminosity functions for the samples
are consistent with previous observations, however we find that the observed
- M relation has a shallow slope more consistent with a constant
mass to light ratio and a normalisation which evolves with redshift. Our
stellar mass functions have steep low-mass slopes (),
steeper than previously observed at these redshifts and closer to that of the
UV luminosity function. Integrating our new mass functions, we find the
observed stellar mass density evolves from at to at . Finally, combining the measured UV continuum
slopes () with their rest-frame UV luminosities, we calculate dust
corrected star-formation rates (SFR) for our sample. We find the specific
star-formation rate for a fixed stellar mass increases with redshift whilst the
global SFR density falls rapidly over this period. Our new SFR density
estimates are higher than previously observed at this redshift.Comment: 28 pages, 23 figures, 2 appendices. Accepted for publication in
MNRAS, August 7 201
Sexual selection protects against extinction
Reproduction through sex carries substantial costs, mainly because only half of sexual adults produce offspring1. It has been theorized that these costs could be countered if sex allows sexual selection to clear the universal fitness constraint of mutation load2,3,4. Under sexual selection, competition between (usually) males and mate choice by (usually) females create important intraspecific filters for reproductive success, so that only a subset of males gains paternity. If reproductive success under sexual selection is dependent on individual condition, which is contingent to mutation load, then sexually selected filtering through ‘genic capture’5 could offset the costs of sex because it provides genetic benefits to populations. Here we test this theory experimentally by comparing whether populations with histories of strong versus weak sexual selection purge mutation load and resist extinction differently. After evolving replicate populations of the flour beetle Tribolium castaneum for 6 to 7 years under conditions that differed solely in the strengths of sexual selection, we revealed mutation load using inbreeding. Lineages from populations that had previously experienced strong sexual selection were resilient to extinction and maintained fitness under inbreeding, with some families continuing to survive after 20 generations of sib × sib mating. By contrast, lineages derived from populations that experienced weak or non-existent sexual selection showed rapid fitness declines under inbreeding, and all were extinct after generation 10. Multiple mutations across the genome with individually small effects can be difficult to clear, yet sum to a significant fitness load; our findings reveal that sexual selection reduces this load, improving population viability in the face of genetic stress.We thank the Natural Environment Research Council and the Leverhulme Trust for financial support, D. Edward for statistical advice and colleagues at the 2013 Biology of Sperm meeting for comments that improved analytical design and interpretation.Peer reviewedPeer Reviewe
The Unusual Infrared Object HDF-N J123656.3+621322
We describe an object in the Hubble Deep Field North with very unusual
near-infrared properties. It is readily visible in Hubble Space Telescope
NICMOS images at 1.6um and from the ground at 2.2um, but is undetected (with
signal-to-noise <~ 2) in very deep WFPC2 and NICMOS data from 0.3 to 1.1um. The
f_nu flux density drops by a factor >~ 8.3 (97.7% confidence) from 1.6 to
1.1um. The object is compact but may be slightly resolved in the NICMOS 1.6um
image. In a low-resolution, near-infrared spectrogram, we find a possible
emission line at 1.643um, but a reobservation at higher spectral resolution
failed to confirm the line, leaving its reality in doubt. We consider various
hypotheses for the nature of this object. Its colors are unlike those of known
galactic stars, except perhaps the most extreme carbon stars or Mira variables
with thick circumstellar dust shells. It does not appear to be possible to
explain its spectral energy distribution as that of a normal galaxy at any
redshift without additional opacity from either dust or intergalactic neutral
hydrogen. The colors can be matched by those of a dusty galaxy at z >~ 2, by a
maximally old elliptical galaxy at z >~ 3 (perhaps with some additional
reddening), or by an object at z >~ 10 whose optical and 1.1um light have been
suppressed by the intergalactic medium. Under the latter hypothesis, if the
luminosity results from stars and not an AGN, the object would resemble a
classical, unobscured protogalaxy, with a star formation rate >~ 100 M_sun/yr.
Such UV-bright objects are evidently rare at 2 < z < 12.5, however, with a
space density several hundred times lower than that of present-day L* galaxies.Comment: Accepted for publication in the Astrophysical Journal. 27 pages,
LaTeX, with 7 figures (8 files); citations & references updated + minor
format change
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