Oxic-anoxic regime shifts mediated by feedbacks between biogeochemical processes and microbial community dynamics

The role of microbial communities in regime shifts is poorly understood. Here, the authors use a mathematical model and field data from a seasonally stratified lake to show that gradual environmental changes can induce oxic-anoxic regime shifts mediated by microbial community dynamics and redox processes

Microbial Community Responses to Vanadium Distributions in Mining Geological Environments and Bioremediation Assessment

Vanadium mining activities can cause contamination of the surrounding geological environment. Vanadium may exist in multiple matrices due to its migration and transformation, forming interactive relationships; however, the connection between vanadium distributions in multiple matrices and microbial community responses remains largely unknown. Vanadium is a redox-sensitive metal that can be microbiologically reduced and immobilized. To date, bioremediation of vanadium-contaminated environments by indigenous microorganisms has rarely been evaluated. This paper reports a systematic investigation into vanadium distributions and microbial communities in soils, water, and sediment from Panzhihua, China. Large vanadium contents of 1130.1 ± 9.8 mg/kg and 0.13 ± 0.02 mg/L were found in surface soil and groundwater. Vanadium in surface water tended to precipitate. Microbial communities isolated from similar environments were alike due to similarity in matrix chemistry whereas communities were distinct when compared to different matrices, with lower richness and diversity in groundwater. Proteobacteria was distributed widely and dominated microbial communities within groundwater. Redundancy analysis shows that vanadium and nutrients significantly affected metal-tolerant bacteria. Long-term cultivation (240 days) suggests the possibility of vanadium bioremediation by indigenous microorganisms, within acid-soluble fraction. This active fraction can potentially release mobile vanadium with shifted redox conditions. Vanadium (V) was bio-reduced to less toxic, mobile vanadium (IV) primarily by enriched Bacillus and Thauera. This study reveals the biogeochemical fate of vanadium in regional geological environments and suggests a bioremediation pathway via native vanadium-reducing microbes

Performance, microbial growth and community interactions of iron-dependent denitrification in freshwaters

Iron-dependent denitrification is a safe and promising technology for nitrogen removal in freshwaters. However, the understanding of microbial physiology and interactions during the process was limited. Denitrifying systems inoculated with freshwater samples were operated with and without iron(II) at a low C/N ratio for 54 days. Iron addition improved nitrogen removal. Batch experiments confirmed that microbially mediated reaction rather than abiotic reaction dominated during the process. Metagenomics recovered genomes of the five most abundant microorganisms, which accounted for over 99% of the community in every triplicate of the iron-based system. Based on codon usage bias, all of them were fast-growing organisms. The total abundance of fast-growing organisms was 38% higher in the system with iron than in the system without iron. Notably, the most abundant organism Diaphorobacter did not have enzymes for asparagine and aspartate biosynthesis, whereas Rhodanobacter could not produce serine and cobalamin. Algoriphagus and Areminomonas lost synthesis enzymes for more amino acids and vitamins. However, they could always obtain these growth-required substances from another microorganism in the community. The two-partner relationship minimized the limitation on microbial reproduction and increased community stability. Our results indicated that iron addition improved nitrogen removal by supplying electron donors, promoting microbial growth, and building up syntrophic interactions among microorganisms with timely communications. The findings provided new insights into the process, with implications for freshwater remediation

Succession of Bacterial Communities in a Seasonally Stratified Lake with an Anoxic and Sulfidic Hypolimnion

Although bacteria play key roles in aquatic food webs and biogeochemical cycles, information on the seasonal succession of bacterial communities in lakes is still far from complete. Here, we report results of an integrative study on the successional trajectories of bacterial communities in a seasonally stratified lake with an anoxic hypolimnion. The bacterial community composition of epilimnion, metalimnion, and hypolimnion diverged during summer stratification and converged when the lake was mixed. In contrast, bacterial communities in the sediment remained relatively stable over the year. Phototrophic Cyanobacteria and heterotrophic Actinobacteria, Alphaproteobacteria and Planktomycetes were abundant in the aerobic epilimnion, Gammaproteobacteria (mainly Chromatiaceae) dominated in the metalimnion, and Chlorobi, Betaproteobacteria, Deltaproteobacteria, and Firmicutes were abundant in the anoxic sulfidic hypolimnion. Anoxic but nonsulfidic conditions expanded to the surface layer during fall turnover, when the epilimnion, metalimnion and upper hypolimnion mixed. During this period, phototrophic sulfur bacteria (Chromatiaceae and Chlorobi) disappeared, Polynucleobacter (Betaproteobacteria) and Methylobacter (Gammaproteobacteria) spread out from the former meta- and hypolimnion to the surface layer, and Epsilonproteobacteria dominated in the bottom water layer. Cyanobacteria and Planktomycetes regained dominance in early spring, after the oxygen concentration was restored by winter mixing. In total, these results show large spatio-temporal changes in bacterial community composition, especially during transitions from oxic to anoxic and from sulfidic to nonsulfidic conditions

Magnetic reed biochar materials as adsorbents for aqueous copper and phenol removal

Organics and heavy metals are common pollutants in many wastewaters and water bodies. Adsorption processes by magnetic materials can rapidly remove these pollutants from water and effectively recycle adsorbent. In this study, magnetic analyzer, X-ray diffraction, Flourier transform infrared spectroscopy, and granulometry were used to characterize the synthesized magnetic reed biochar materials (ZnFeO/biochar). Influences of adsorption time, pH, temperature, initial solution concentration, and adsorption equilibrium concentration on adsorption performances were investigated for Cu and phenol adsorption by ZnFeO/biochar. Adsorption kinetic and isotherm models were used to describe the adsorption processes. Adsorption of phenol and Cu by ZnFeO/biochar reached saturation within 45 min and increased slightly with the increase of temperature from 15 to 45 °C. Adsorption of Cu increased with the increase of pH, while the adsorption of phenol peaked at pH = 6. The adsorption processes fit the pseudo-second order kinetics model, and both conformed to the Langmuir model. The fitting results show that the maximum single-component adsorption capacity of phenol and Cu by ZnFeO/biochar is 63.29 and 12.20 mg/g, and the maximum bi-component adsorption capacity reaches 40.16 and 9.48 mg/g, respectively. All the findings demonstrate that ZnFeO/biochar has good adsorption performance for phenol and Cu

Microbial community structure and function in Canadian Prairie groundwater ecosystems.

Groundwater ecosystems are globally widespread yet still poorly understood. The goal of the study was to understand the links between the biogeochemistry and microbial ecology of groundwater ecosystems in diverse geological settings on a broad spatial scale. We investigated the age, aqueous geochemistry, and microbiology of >100 groundwater samples from 90 monitoring wells (<250m depth) located in 14 aquifers in the Canadian Prairie. Geochemistry and microbial ecology were tightly linked revealing large-scale aerobic and anaerobic hydrogen, methane, nitrogen, and sulfur cycling carried out by diverse microbial communities. Details concerning methods, results and conclusions can be found in the associated publication by Ruff et al. 2023

Contextual and environmental data characterizing 138 samples from Canadian groundwater ecosystems

Groundwater ecosystems are globally widespread yet still poorly understood. We therefore investigated contextual and environmental data characterizing 138 groundwater samples from Canadian groundwater ecosystems. The chemical, physical, gas, isotopic, and microbiological measurements were taken from aquifers in the Canadian Prairie between 2015 and 2020. The study area comprised 14 major aquifers and a geographic area of ~210.000 km2. The goal of the study was to understand the links between the biogeochemistry and microbial ecology of groundwater ecosystems in diverse geological settings on a broad spatial scale. Details concerning methods, results and conclusions can be found in the associated publication by Ruff et al. 2023

Nucleotide sequences of archaeal amplicon sequence variants (ASV) from Canadian groundwater ecosystems

Groundwater ecosystems are globally widespread yet still poorly understood. We investigated the microbiology of >100 groundwater samples from 90 monitoring wells (<250m depth) located in 14 aquifers in the Canadian Prairie. This dataset contains the nucleotide sequences and taxonomy of archaeal 16S rRNA gene amplicons retrieved from groundwater samples of aquifers in the Canadian Prairie, collected between 2015 and 2020. The study area comprised 14 major aquifers and a geographic area of ~210.000 km2. The goal of the study was to understand the links between the biogeochemistry and microbial ecology of groundwater ecosystems in diverse geological settings on a broad spatial scale. Details concerning DNA extraction, sequencing and sequence analyses can be found in the associated publication by Ruff et al. 2023