Surrealism with Floating Origami Rose: A Cocktail Ensemble

Surrealism with Floating Origami Rose: A Cocktail Ensemble Diane Sparks and Tyler Klene, Colorado State University The focus in this submission is the Surrealist landscape textile design. Emphasis was achieved through digitally manipulating color vibrancy and contrast in the artwork used for the silk textile. Garment structures were held at a minimum level of complexity to emphasize the textile design. Traditional draping techniques were used to create garment patterns, and couture techniques were used to assemble and finish garment structures. This design follows the precedent of a relationship between fashion and Surrealism established in the 1930’s by Elsa Schiaparelli and Salvador Dalí. It is different, in that the textile design process was done using digital technology. The contribution to the literature provided by this submission, is a unique textile design and garment structure that visually demonstrate the potential for dress designing to be a manifestation of art

Constraining the Size of Near Earth Asteroids

Quick and accurate determination of the size of an asteroid is of great interest to the Asteroid Threat Assessment Project and is difficult to accomplish. With a combination of visible and thermal measurements we employ a method that leverages the size estimations of each model as physical constraints on the true diameter. This method breaks degeneracies present in the thermal and visible model from sparse data. In the visible bands we use both the established H-G relationship and its successor the H-G1G2 model, which has improved capabilities in the opposition effect and large phase angles. For the thermal models we use the Near Earth Asteroid Thermal Model (NEATM), the Night Emission Simulated Thermal Model (NESTM), and the Advanced Thermophysical Model (ATPM)

Spatial mapping of hematopoietic clones in human bone marrow

UNLABELLED: Clonal hematopoiesis (CH) is the expansion of somatically mutated cells in the hematopoietic compartment of individuals without hematopoietic dysfunction. Large CH clones (i.e., \u3e2% variant allele fraction) predispose to hematologic malignancy, but CH is detected at lower levels in nearly all middle-aged individuals. Prior work has extensively characterized CH in peripheral blood, but the spatial distribution of hematopoietic clones in human bone marrow is largely undescribed. To understand CH at this level, we developed a method for spatially aware somatic mutation profiling and characterized the bone marrow of a patient with polycythemia vera. We identified the complex clonal distribution of somatic mutations in the hematopoietic compartment, the restriction of somatic mutations to specific subpopulations of hematopoietic cells, and spatial constraints of these clones in the bone marrow. This proof of principle paves the way to answering fundamental questions regarding CH spatial organization and factors driving CH expansion and malignant transformation in the bone marrow. SIGNIFICANCE: CH occurs commonly in humans and can predispose to hematologic malignancy. Although well characterized in blood, it is poorly understood how clones are spatially distributed in the bone marrow. To answer this, we developed methods for spatially aware somatic mutation profiling to describe clonal heterogeneity in human bone marrow. See related commentary by Austin and Aifantis, p. 139

Neutrophils are Mediators of Metastatic Prostate Cancer Progression in Bone

Bone metastatic prostate cancer (BM-PCa) significantly reduces overall patient survival and is currently incurable. Current standard immunotherapy showed promising results for PCa patients with metastatic, but less advanced, disease (i.e., fewer than 20 bone lesions) suggesting that PCa growth in bone contributes to response to immunotherapy. We found that: (1) PCa stimulates recruitment of neutrophils, the most abundant immune cell in bone, and (2) that neutrophils heavily infiltrate regions of prostate tumor in bone of BM-PCa patients. Based on these findings, we examined the impact of direct neutrophil-prostate cancer interactions on prostate cancer growth. Bone marrow neutrophils directly induced apoptosis of PCa in vitro and in vivo, such that neutrophil depletion in bone metastasis models enhanced BM-PCa growth. Neutrophil-mediated PCa killing was found to be mediated by suppression of STAT5, a transcription factor shown to promote PCa progression. However, as the tumor progressed in bone over time, neutrophils from late-stage bone tumors failed to elicit cytotoxic effector responses to PCa. These findings are the first to demonstrate that bone-resident neutrophils inhibit PCa and that BM-PCa are able to progress via evasion of neutrophil-mediated killing. Enhancing neutrophil cytotoxicity in bone may present a novel therapeutic option for bone metastatic prostate cancer

TEF, Vol. 2 No. 1

The second annual literary anthology of students writing from Stephen F Austin State College.https://scholarworks.sfasu.edu/tef/1001/thumbnail.jp

Subject benchmark statement: healthcare programmes, phase 2: Arts Therapy, QAA 05909/04

sch_occpub2062pu

Field-deployable, quantitative, rapid identification of active Ebola virus infection in unprocessed blood

The West African Ebola virus outbreak underlined the importance of delivering mass diagnostic capability outside the clinical or primary care setting in effectively containing public health emergencies caused by infectious disease. Yet, to date, there is no solution for reliably deploying at the point of need the gold standard diagnostic method, real time quantitative reverse transcription polymerase chain reaction (RT- qPCR), in a laboratory infrastructure-free manner. In this proof of principle work, we demonstrate direct performance of RT-qPCR on fresh blood using far-red fluorophores to resolve fluorogenic signal inhibition and controlled, rapid freeze/thawing to achieve viral genome extraction in a single reaction chamber assay. The resulting process is entirely free of manual or automated sample pre-processing, requires no microfluidics or magnetic/mechanical sample handling and thus utilizes low cost consumables. This enables a fast, laboratory infrastructure-free, minimal risk and simple standard operating procedure suited to frontline, field use. Developing this novel approach on recombinant bacteriophage and recombinant human immunodeficiency virus (HIV; Lentivirus), we demonstrate clinical utility in symptomatic EBOV patient screening using live, infectious Filoviruses and surrogate patient samples. Moreover, we evidence assay co-linearity independent of viral particle structure that may enable viral load quantification through pre-calibration, with no loss of specificity across an 8 log- linear maximum dynamic range. The resulting quantitative rapid identification (QuRapID) molecular diagnostic platform, openly accessible for assay development, meets the requirements of resource- limited countries and provides a fast response solution for mass public health screening against emerging biosecurity threats

Effectiveness of a tailored training programme in behaviour change counselling for community pharmacists: A pilot study

Objective: To undertake a pilot study assessing effectiveness of a tailored training programme in behaviour change counselling (BCC) for community pharmacists on, their competence and confidence in delivering behaviour change consultations, skill retention over time and impact on practice. Methods: Community pharmacists (N = 87) attending Primary Care Trust training were given study information and invited to take part. Baseline BCC competence of consenting pharmacists (n = 17) was assessed using the Behaviour Change Counselling Index (BECCI). Following BCC training, competence was reassessed at 1, 3 and 6 months. Friedman’s test was used to compare median BECCI item scores at baseline and after 6 months. Structured interviews were conducted to assess pharmacists’ confidence in BCC consultations after training. Results: Baseline BECCI scores of 0–2 demonstrated pharmacists had not reached competence threshold. Six months after training, BECCI scores improved significantly from baseline (p < 0.05). Competence in delivering BCC (scores of 3–4) was achieved at 3 months, but lost at 6 months for some items. After training, pharmacists felt confident in delivering BCC. Conclusion: Training pharmacists enabled them to deliver BCC competently and confidently. Practice implications: BCC aligns with pharmacist-patient consultations. It took 3 months to achieve competence. Ongoing support may be needed to maintain competence long-term

Expressions 1984

https://openspace.dmacc.edu/expressions/1008/thumbnail.jp

Increased SIRT3 combined with PARP inhibition rescues motor function of SBMA mice.

Spinal and bulbar muscular atrophy (SBMA) is a neuromuscular disease with substantial mitochondrial and metabolic dysfunctions. SBMA is caused by polyglutamine (polyQ) expansion in the androgen receptor (AR). Activating or increasing the NAD+-dependent deacetylase, SIRT3, reduced oxidative stress and death of cells modeling SBMA. However, increasing diminished SIRT3 in AR100Q mice failed to reduce acetylation of the SIRT3 target/antioxidant, SOD2, and had no effect on increased total acetylated peptides in quadriceps. Yet, overexpressing SIRT3 resulted in a trend of motor recovery, and corrected TCA cycle activity by decreasing acetylation of SIRT3 target proteins. We sought to boost blunted SIRT3 activity by replenishing diminished NAD+ with PARP inhibition. Although NAD+ was not affected, overexpressing SIRT3 with PARP inhibition fully restored hexokinase activity, correcting the glycolytic pathway in AR100Q quadriceps, and rescued motor endurance of SBMA mice. These data demonstrate that targeting metabolic anomalies can restore motor function downstream of polyQ-expanded AR