Infectious agents and inflammation. The role of microbiota in autoimmune arthritis

In higher vertebrates, mucosal sites at the border between the internal and external environments, directly interact with bacteria, viruses, and fungi. Through co-evolution, hosts developed mechanisms of tolerance or ignorance toward some infectious agents, because hosts established "gain of function" interactions with symbiotic bacteria. Indeed, some bacteria assist hosts in different functions, among which are digestion of complex carbohydrates, and absorption and supply of vitamins. There is no doubt that microbiota modulate innate and acquired immune responses starting at birth. However, variations in quality and quantity of bacterial species interfere with the equilibrium between inflammation and tolerance. In fact, correlations between gut bacteria composition and the severity of inflammation were first described for inflammatory bowel diseases and later extended to other pathologies. The genetic background, environmental factors (e.g., stress or smoking), and diet can induce strong changes in the resident bacteria which can expose the intestinal epithelium to a variety of different metabolites, many of which have unknown functions and consequences. In addition, alterations in gut permeability may allow pathogens entry, thereby triggering infection and/or chronic inflammation. In this context, a local event occurring at a mucosal site may be the triggering cause of an autoimmune reaction that eventually involves distant sites or organs. Recently, several studies attributed a pathogenic role to altered oral microbiota in rheumatoid arthritis (RA) and to gut dysbiosis in spondyloarthritis (SpA). There is also growing evidence that different drugs, such as antibiotics and immunosuppressants, can influence and be influenced by the diversity and composition of microbiota in RA and SpA patients. Hence, in complex disorders such RA and SpA, not only the genetic background, gender, and immunologic context of the individual are relevant, but also the history of infections and the structure of the microbial community at mucosal sites should be considered. Here the role of the microbiota and infections in the initiation and progression of chronic arthritis is discussed, as well as how these factors can influence a patient's response to synthetic and biologic immunosuppressive therapy

Dark matter protohalos in MSSM-9 and implications for direct and indirect detection

We study how the kinetic decoupling of dark matter (DM) within a minimal supersymmetric extension of the standard model, by adopting nine independent parameters (MSSM-9), could improve our knowledge of the properties of the DM protohalos. We show that the most probable neutralino mass regions, which satisfy the relic density and the Higgs mass contraints, are those with the lightest supersymmetric neutralino mass around 1 TeV and 3 TeV, corresponding to Higgsino-like and Wino-like neutralino, respectively. The kinetic decoupling temperature in the MSSM-9 scenario leads to a most probable protohalo mass in a range of $M_{\mathrm{ph}}\sim 10^{-12}-10^{-7}\,M_\odot$. The part of the region closer to 2 TeV gives also important contributions from the neutralino-stau co-annihilation, reducing the effective annihilation rate in the early Universe. We also study how the size of the smallest DM substructures correlates to experimental signatures, such as the spin-dependent and spin-independent scattering cross sections, relevant for direct detection of DM. Improvements on the spin-independent sensitivity might reduce the most probable range of the protohalo mass between $\sim$10$^{-9}\,M_\odot$ and $\sim$10$^{-7}\,M_\odot$, while the expected spin-dependent sensitivity provides weaker constraints. We show how the boost of the luminosity due to DM annihilation increases, depending on the protohalo mass. In the Higgsino case, the protohalo mass is lower than the canonical value often used in the literature ($\sim$10$^{-6}\,M_\odot$), while $\langle\sigma v\rangle$ does not deviate from $\langle\sigma v\rangle\sim 10^{-26}$ cm$^3$ s$^{-1}$; there is no significant enhancement of the luminosity. On the contrary, in the Wino case, the protohalo mass is even lighter, and $\langle\sigma v\rangle$ is two orders of magnitude larger; as its consequence, we see a substantial enhancement of the luminosity.Comment: 26 pages, 8 figure

Modellazione molecolare di inibitori organici nel cemento (Molecular modeling of organic inhibitors in concrete)

Corrosion inhibitors are largely used to prevent chloride-induced corrosion in reinforced concrete structures. The interaction mechanisms with the passive film present on steel still requires deeper understanding. In a previous work [1] based on molecular mechanics and molecular dynamics methods [2-5] we considered organic inhibitors adsorbed on γ-FeOOH, comparing theoretical results with experimental data [1]. Here we considered the initial interaction with the inhibitor film and chlorides. In particular, the adsorbed tartrate monolayer show the best behavior thanks to the repulsions by the COO- groups exposed to chlorides, more distant from the γ-FeOOH surface, whereas the dimethylethanolamine film doesn’t have the same repulsion. The molecular simulations are a useful tool to better understand the behaviour of inhibitors in presence of chlorides that can start the corrosio

TCD4pos lymphocytosis in rheumatoid and psoriatic arthritis patients following TNFα blocking agents

BACKGROUND: Lymphocyte expansion and true lymphocytosis are commonly observed in the everyday clinical practice. The meaning of such phenomenon is often poorly understood so that discrimination between benign and malignant lymphocytosis remains difficult to establish. This is mainly true when lymphocytosis rises in patients affected by immune-mediated chronic inflammatory diseases under immunosuppressive treatment, conditions potentially associated with lymphomagenesis. In this brief report the development of mild T CD4pos lymphocytosis in a group of patients with chronic arthritis under anti-TNF-α treatment is described. METHODS: Two hundred eight rheumatoid arthritis (RA) and psoriatic arthritis (PsA) patients have been evaluated longitudinally for at least 1-year before and 2-years after anti-TNF-α therapy introduction for the possible appearance of a lymphocyte expansion. In patients who developed lymphocyte expansion, T, B and NK cells were analysed. RESULTS: Twenty-five out of 208 (12%) subjects developed a mild T CD4pos lymphocytosis, during anti-TNF-α therapy, which reverted after its interruption. Higher lymphocyte count, more frequent use of steroids and shorter disease duration, before biological therapy start, have emerged as risk factors for lymphocytosis development. CONCLUSIONS: This is the first longitudinal cohort study evaluating the onset of lymphocytosis in RA and PsA patients under anti-TNF-α treatment and its possible clinical relevance. A mild T CD4pos lymphocytosis has been observed in 12% of RA and PsA patients probably related to anti-TNF-α treatment as previously reported by anecdotal cases. Patients with higher baseline lymphocyte count, use of steroids and shorter disease duration before the introduction of biologic therapy, seem to be prone to develop this laboratory reversible abnormality

Dark radiation and interacting scenarios

An extra dark radiation component can be present in the universe in the form of sterile neutrinos, axions or other very light degrees of freedom which may interact with the dark matter sector. We derive here the cosmological constraints on the dark radiation abundance, on its effective velocity and on its viscosity parameter from current data in dark radiation-dark matter coupled models. The cosmological bounds on the number of extra dark radiation species do not change significantly when considering interacting schemes. We also find that the constraints on the dark radiation effective velocity are degraded by an order of magnitude while the errors on the viscosity parameter are a factor of two larger when considering interacting scenarios. If future Cosmic Microwave Background data are analyzed assuming a noninteracting model but the dark radiation and the dark matter sectors interact in nature, the reconstructed values for the effective velocity and for the viscosity parameter will be shifted from their standard 1/3 expectation, namely c(eff)(2) = 0.34(-0.003)(+0.006) and c(vis)(2) = 0.29(-0.001)(+0.002) at 95% C.L. for the future COrE mission data

Students and COVID-19 Pandemic: Learning Engagement and Social Dialectic of Space – Research on Students at the University of Ioannina in Greece

The COVID-19 pandemic in March 2020 also caused the closure of all educational institutions in Greece. Higher education implemented the distance education system for the first time. In the new educational environment, all the active interactions of students’ learning engagement were put to the test. Learning engagement is directly linked to the suitability and functionality of the study space. Space is the result of a series and set of functions, and sometimes it allows new actions to happen, sometimes it suggests others, and sometimes it forbids them. During the pandemic, it was an important criterion for the learning process, for quality and demanding student learning. A quantitative survey conducted in April 2023 on a sample of 537 students from 21 Departments of the University of Ioannina showed that the family environment, the number of bedrooms, the functionality of the space and the economic level of the family are factors that played an important role in the learning engagement and involvement of the students. The research findings showed the positive correlation of student study space functionality with financial capital and learning engagement, issues analysed in this paper