L'Osservatorio Geofisico di Lipari ed i 40 anni della Rete Sismica Permanente del Tirreno Meridionale

In 1966, the Istituto Internazionale di Vulcanologia (I.I.V.), gave the go ahead to the ambitious project of setting up a seismic network in the southern Tyrrhenian Sea, an area of extraordinary geophysical interest, which until then was not covered by seismic monitoring. At the time, there were single seismic stations on the rest of the italian peninsula. These were inside universities, public and private bodies and at the numerous observatories of the Istituto Nazionale di Geofisica (founded in 1936 by Antonino Lo Surdo and becoming independent of the Consiglio Nazionale delle Ricerche (C.N.R,. in 1945) and still used mechanical seismographs, with masses weighing from 80 up to 1300 Kg. On the Aeolian Islands instead, electromagnetic seismometers were employed from the start. The experimental network, planned in the Willmore laboratories at Edinburgh and tested in the Aeolian Islands over a two-year period, represented the starting point for the Permanent Seismic Network (PSN) of the Southern Tyrrhenian. After the experience of the Laboratorio Internazionale per le Ricerche Vulcanologiche (L.I.R.V. - C.N.R.), of the Istituto di Vulcanologia of the University of Catania, the independent I.I.V. - C.N.R., together with the observatory, was launched in 1969. In the course of the seventies, every island of the Aeolian archipelago, was equipped with at least a seismic station and the 3 sensor systems (triaxial systems) began to become widely used. In the eighties, the “Sismologia Eolie” project was started, aimed at a further develop and update the seismic net. With the wide use of magnetic tape recording, the instrumental data stored could be more readily transported on suitable drums to the main centre in Catania for analysis. Here, Research Units were established in order to prepare the way for enhanced interaction between technical staff and research groups. The instrumental geophysical investigations, progressively extended and in a permanente form to Eastern Sicily and particular around Etna. From the nineties, operations room in Catania was fully active, working 24/24, and ensuring the seismic and volcanic surveillance of Etna, Stromboli and Vulcano. The Geophysical Observatory of Lipari and the main centre of the Institute in Catania, were merged in 2001 into the new Istituto Nazionale di Geofisica e Vulcanologia (INGV) and the network of the Aeolian Islands became an integral part of the Permanent Seismic Network (PSN) of Eastern Sicily and Southern Calabria. Special prominence will be given in the course of this historical reconstruction to the evolution of instrumental seismology, thanks to forty years of continuous input, indispensable for the understanding of the seismogenetic processes in the area, this still represents the chief undertaking of the geophysical observatory on Lipari, whose important scientific but also social role must be acknowledged in the growing interaction between research and civil protection

Improvement of resveratrol permeation through sublingual mucosa: Chemical permeation enhancers versus spray drying technique to obtain fast-disintegrating sublingual mini-tablets

Resveratrol (RSV) is a natural polyphenol with several interesting broad-spectrum pharmacological properties. However, it is characterized by poor oral bioavailability, extensive first-pass effect metabolism and low stability. Indeed, RSV could benefit from the advantage of the sublingual route of administration. In this view, RSV attitudes to crossing the porcine sublingual mucosa were evaluated and promoted both by six different chemical permeation enhancers (CPEs) as well as by preparing four innovative fast-disintegrating sublingual mini-tablets by spray drying followed by direct compression. Since RSV by itself exhibits a low permeation aptitude, this could be significantly enhanced by the use of CPEs as well as by embedding RSV in a spray-dried powder to be compressed in order to prepare fast-disintegrating mini-tablets. The most promising observed CPEs (menthol, lysine and urea) were then inserted into the most promising spray-dried excipients’ compositions (RSV-B and RSV-C), thus preparing CPE-loaded mini-tablets. However, this procedure leads to unsatisfactory results which preclude the possibility of merging the two proposed approaches. Finally, the best spray-dried composition (RSV-B) was further evaluated by SEM, FTIR, XRD and disintegration as well as dissolution behavior to prove its effectiveness as a sublingual fast-disintegrating formulation

Straw mulch as a sustainable solution to decrease runoff and erosion in glyphosate-treated clementine plantations in Eastern Spain. An assessment using rainfall simulation experiments

[EN] In many Mediterranean areas, citrus orchards exhibit high soil loss rates because of the expansion of drip irrigation that allows cultivation on sloping terrain and the widespread use of glyphosate. To mitigate these non-sustainable soil losses, straw mulch could be applied as an efficient solution but this has been poorly studied. Therefore, the main goal of this paper was to assess the use of straw mulch as a tool to reduce soil losses in clementine plantations, which can be considered representative of a typical Mediterranean citrus orchard. A total of 40 rainfall simulation experiments were carried out on 20 pairs of neighbouring bare and mulched plots. Each experiment involved applying 38.8 mm of rain at a constant rate over 1 h to a circular plot of 0.28 m(2) circular plots. The results showed that a cover of 50% of straw (60 g m(-2)) was able to delay the time to ponding from 32 to 52 s and the time to runoff initiation from 57 to 129 s. Also, the mulching reduced the runoff coefficient from 65.6 to 50.5%. The effect on sediment transport was even more pronounced, as the straw mulch reduced the sediment concentration from 16.7 g l(-1) to 3.6 g l(-1) and the soil erosion rates from 439 g to 73 g. Our results indicated that mulching can be used as a useful management practice to control soil erosion rates due to the immediate effect on high soil detachment rate and runoff initiation reduction in conventional clementine orchards on sloping land, by slowing down runoff initiation and by reducing runoff generation and, especially, sediment losses. We indirectly concluded that straw mulch is also a sustainable solution in glyphosate-treated citrus plantations.This paper is part of the results of research projects GL2008-02879/BTE, LEDDRA 243857 and RECARE-FP7 (ENV.2013.6.2-4).Keesstra, S.; Rodrigo-Comino, J.; Novara, A.; Giménez Morera, A.; Pulido, M.; Di Prima, S.; Cerda, A. (2019). Straw mulch as a sustainable solution to decrease runoff and erosion in glyphosate-treated clementine plantations in Eastern Spain. An assessment using rainfall simulation experiments. CATENA. 174:95-103. https://doi.org/10.1016/j.catena.2018.11.007S9510317

Diagnostic accuracy of fecal calprotectin assay in distinguishing organic causes of chronic diarrhea from irritable bowel syndrome: a prospective study in adults and children

Fecal calprotectin (FC) has been proposed as a marker of inflammatory bowel disease (IBD), but few studies have evaluated its usefulness in patients with chronic diarrhea of various causes. We evaluated the diagnostic accuracy of a FC assay in identifying "organic" causes of chronic diarrhea in consecutive adults and children

Anti-Transglutaminase Antibody Assay of the Culture Medium of Intestinal Biopsy Specimen Can Improve the Accuracy of Celiac Disease Diagnosis.

BACKGROUND: We measured anti-transglutaminase (anti-tTG) antibody in the culture medium of intestinal biopsy specimens from patients with suspected celiac disease (CD) and evaluated the relationship between antibody production and severity of intestinal mucosal damage. METHODS: We performed diagnostic testing for CD on 273 consecutive patients. In addition to routine histologic evaluation of duodenal biopsy specimens, we assayed anti-tTG antibodies in serum and in the culture medium of duodenal biopsy specimens. RESULTS: CD was diagnosed in 191 of the 273 patients. Sensitivity and specificity of the serum anti-endomysium (EmA) and anti-tTG assays were 83% and 85% and 99% and 95%, respectively, and both had 88% diagnostic accuracy. EmA and anti-tTG assayed in the culture medium had 98% sensitivity, 100% specificity, and 98% diagnostic accuracy (vs serum assays; P <0.0001). Twenty-nine CD patient specimens (16%) were negative for serum anti-tTG and EmA; for 24 of these patients, anti-tTG assay of the culture medium was positive. The CD patients whose biopsy specimens were positive for serum antibodies showed the following intestinal histologies: total villous atrophy, 35%; severe villous atrophy, 25%; mild atrophy, 25%; villi with no atrophy but with increased intraepithelial lymphocytes, 15%. None of the CD patients whose specimens were negative for serum antibodies showed total or severe villous atrophy; 77% had mild villous atrophy, and 23% had no villous atrophy but had increased intraepithelial lymphocyte counts. Mild villous atrophy was also seen in specimens from approximately 15% of patients without CD. CONCLUSION: Anti-tTG assay of the culture medium of biopsy specimens can improve the accuracy of CD diagnosis in patients negative for serum antibodies

Lipid nanocarriers-loaded nanocomposite as a suitable platform to release antibacterial and antioxidant agents for immediate dental implant placement restorative treatment

Immediate implant placement is a single-stage restorative approach for missing teeth widely used to overcome the ridge remodeling process occurring after dental extractions. The success of this procedure relies on opportune osseointegration in the surrounding tissues. To support this process, a multifunctional nanocomposite, to be applied in the fresh post-extraction socket, was here designed, prepared, and characterized. This formulation consists of quercetin (QRC)-loaded nanostructured lipid carriers (NLCs) entrapped in a chitosan-based solid matrix containing ciprofloxacin (CPX). QRC-NLCs were prepared by homogenization followed by high-frequency sonication, and thereafter this dispersion was trapped in a chitosan-based CPX-loaded gel, obtaining the nanocomposite powder (BioQ-CPX) by lyophilization. BioQ-CPX displayed desirable properties such as high porosity (94.1 ± 0.5%), drug amounts (2.1% QRC and 3.5% CPX). and low swelling index (100%). Moreover, the mechanism of drug release from BioQ-CPX and their ability to be accumulated in the target tissue were in vitro and ex vivo elucidated, also by applying mathematical models. When trapped into the nanocomposite, QRC stressed under UV light exposure (50 W) was shown to maintain its antioxidant power, and CPX and QRC under natural light were stable over nine months. Finally, both the measured antioxidant power and the antimicrobial and antibiofilm properties on Staphylococcus aureus demonstrated that BioQ-CPX could be a promising platform to support the single-stage dental restorative treatment

Usefulness of the organ culture system in the in vitro diagnosis of coeliac disease: A multicentre study

Objective. Diagnosis of coeliac disease is based on the presence of villous atrophy which recovers following a gluten-free diet. The presence of circulating antiendomysial antibodies as well as their disappearance after a gluten-free diet supports the diagnosis. It has also been demonstrated that antiendomysial antibodies are detectable in supernatants of cultured intestinal biopsies from patients with coeliac disease. The objective of this study was to compare the histology and antiendomysial antibodies in culture supernatants of intestinal biopsies to validate the in vitro organ culture system as a future diagnostic tool for coeliac disease. Material and methods. Seventy-five antiendomysial serum-positive patients on a gluten-containing diet were evaluated. Patients underwent endoscopy with 5 biopsy fragments: 3 for histology, 1 cultured with and the other without gliadin-peptide activator. Antiendomysial antibodies were evaluated in all culture supernatants. Results. Sixty-eight patients had evidence of villous atrophy, while 73 out of 75 were positive to the organ culture system. The agreement rate between organ culture and histology results was 94%. Conclusions. As all the centres participating in the study obtained good agreement between organ culture and histology results, the new system could be considered a reliable tool for the diagnosis of coeliac disease. Nevertheless, it is possible to highlight cases with an organ culture-positive and -negative histology. This feature could be of considerable interest because, as the sensitivity of organ culture seems to be greater than the initial histology, the new system might be useful in uncertain cases where the risk of missing the diagnosis of coeliac disease is high

La agricultura ecológica a largo plazo en plantaciones de cítricos permite la recuperación del carbono orgánico del suelo

[ES] Se ha demostrado que el manejo del suelo bajo agricultura ecológica puede aumentar el contenido de carbono orgánico en el suelo moderando el incremento de los gases de efecto invernadero, pero hasta la fecha las evaluaciones cuantitativas basadas en mediciones a largo plazo han sido escasas, especialmente bajo condiciones mediterráneas. En esta investigación se examinaron los cambios en el contenido de carbono orgánico como respuesta a la agricultura ecológica con cobertura vegetal en una plantación de cítricos en el área mediterránea, utilizando una base de datos de 21 años. El incremento de contenido de carbono orgánico en el suelo fue más evidente tras cinco años desde el cambio del manejo del suelo, sugiriendo que, para plantaciones de cítricos en ambientes mediterráneos, los estudios deberían tener una duración superior a cinco años. La sata de secuestro de carbono orgánico no cambió significativamente durante los 21 años de observaciones, con valores que oscilaron entre -1.10 Mg C ha-1 a-1 y 1.89 Mg C ha-1 a-1. Tras 21 años, un total de 61 t CO2 ha-1 fueron secuestradas en las áreas de acumulación de carbono en el suelo. Estos resultados demuestran que la agricultura ecológica es una estrategia efectiva para restaurar o incrementar los niveles de carbono orgánico en el suelo en los sistemas de cítricos mediterráneos.[EN] It has been shown that soil management under organic farming can enhance soil organic carbon, thereby mitigating atmospheric greenhouse gas increases, but until now quantitative evaluations based on long term experiments are scarce, especially under Mediterranean conditions. Changes in soil organic carbon (SOC) content were examined in response to organic management with cover crops in a Mediterranean citrus plantation using 21 years of survey data. Soil organic carbon increase was more apparent 5 years after a land management change suggesting that, for citrus plantations on Mediterranean conditions, studies should be longer than five years in duration. Soil organic carbon sequestration rate did not significantly change during the 21 years of observation, with values ranging from -1.10 Mg C ha(-1) y(-1) to 1.89 Mg C ha(-1) y(-1). After 21 years, 61 Mg CO2 ha(-1) were sequestered in long-lived soil C pools. These findings demonstrate that organic management is an effective strategy to restore or increase SOC content in Mediterranean citrus systems.This research was funded by the European Union Seventh Framework Program (FP7/2007-2013) under grant no. 603498 (RECARE Project) and the research projects GL2008-02879/BTE and LEDDRA 243857.Novara, A.; Pulido, M.; Rodrigo-Comino, J.; Di Prima, S.; Smith, P.; Gristina, L.; Giménez Morera, A.... (2019). Long-term organic farming on a citrus plantation results in soil organic carbon recovery. Cuadernos de Investigación Geográfica. 45(1):271-286. https://doi.org/10.18172/cig.3794S27128645