Visualization of a Small Jet Synthetic Using a Particle Image Velocimetry and Background-Oriented Schlieren Techniques

This work reports the results of experimental investigations carried out on a submerged synthetic jet obtained through the use of a headset speaker piloted with an appropriate sinusoidal signal at a frequency of 380 Hz. The study begins with the construction of a device that, exploiting the well-known transport properties of impacting jets, was able to improve local ventilation and the removal of excess moisture, due to the natural transpiration of human skin in people forced to assume the same position for prolonged periods such as professional drivers; bedridden patients; etc… Subjects are substantially forced to have parts of their own body in contact with fabrics and coverings that hinder the normal conditions of skin transpiration. The experimental activity was first based on the study of the structure of the synthetic jets, then moving on to the creation of a sponge mat equipped with 80 individual jets. On this sponge mat, semi-empirical tests were carried out in order to remove moisture from a fabric soaked in distilled water. The experimental investigations were first carried out using the PIV technique, and, subsequently, the synthetic jet was visualized using the Background-Oriented Schlieren (BOS) technique which allowed to test the presence of the jets, installed directly on the mattress, in a relatively simple and fast way, requiring a very simplified set-up

Pain control in the continuity of care.

As cancer is earlier diagnosed and its treatments improve, palliative care is increasingly playing a vital role in the oncology population. The concept and the timing of application of palliative care have evolved in the last decades. The WHO pain ladder and the greater understanding of appropriate multimodal pain control treatments have dramatically improved the management of cancer pain. Integration of palliative care, which appears crucial for a proper management of patients, can be defined as the provision of palliative care both during curative cancer treatment and after curative treatment has ceased. Clinical assistance should be delivered by specialized physicians in different fields, psychologists and nurses, and should include all aspects of advanced cancer care, from diagnosis to the treatment of symptoms. A further aspect of integration of palliative care concerns the role of the continuity of care in acute or emergency contexts both for out- and inpatients. Further improvements in the management of cancer pain are needed. First, the WHO ladder should be modified with further steps, like those of interventional pain control procedures and techniques, with the aim of being effective also for the small proportion of nonresponsive patients. Second, more research is needed to find out which interventions aiming to improve continuity of care of cancer patients are beneficial to improve patient, provider and process of care outcomes and to identify which outcomes are the most sensitive to change. Of crucial importance would be the development of a standardised instrument to measure the continuity of care in cancer patients. This article is a brief overview on the management of cancer pain, from the pharmacological treatments reported by WHO ladder, to the need for integration and continuity of care

Semiconductor-based Geometrical Quantum Gates

We propose an implementation scheme for holonomic, i.e., geometrical, quantum information processing based on semiconductor nanostructures. Our quantum hardware consists of coupled semiconductor macroatoms addressed/controlled by ultrafast multicolor laser-pulse sequences. More specifically, logical qubits are encoded in excitonic states with different spin polarizations and manipulated by adiabatic time-control of the laser amplitudes . The two-qubit gate is realized in a geometric fashion by exploiting dipole-dipole coupling between excitons in neighboring quantum dots.Comment: 4 Pages LaTeX, 3 Figures included. To appear in PRB (Rapid Comm.

FRA-1 protein overexpression is a feature of hyperplastic and neoplastic breast disorders

BACKGROUND: Fos-related antigen 1 (FRA-1) is an immediate early gene encoding a member of AP-1 family of transcription factors involved in cell proliferation, differentiation, apoptosis, and other biological processes. fra-1 gene overexpression has an important role in the process of cellular transformation, and our previous studies suggest FRA-1 protein detection as a useful tool for the diagnosis of thyroid neoplasias. Here we investigate the expression of the FRA-1 protein in benign and malignant breast tissues by immunohistochemistry, Western blot, RT-PCR and qPCR analysis, to evaluate its possible help in the diagnosis and prognosis of breast neoplastic diseases. METHODS: We investigate the expression of the FRA-1 protein in 70 breast carcinomas and 30 benign breast diseases by immunohistochemistry, Western blot, RT-PCR and qPCR analysis. RESULTS: FRA-1 protein was present in all of the carcinoma samples with an intense staining in the nucleus. Positive staining was also found in most of fibroadenomas, but in this case the staining was present both in the nucleus and cytoplasm, and the number of positive cells was lower than in carcinomas. Similar results were obtained from the analysis of breast hyperplasias, with no differences in FRA-1 expression level between typical and atypical breast lesions; however the FRA-1 protein localization is mainly nuclear in the atypical hyperplasias. In situ breast carcinomas showed a pattern of FRA-1 protein expression very similar to that observed in atypical hyperplasias. Conversely, no FRA-1 protein was detectable in 6 normal breast tissue samples used as controls. RT-PCR and qPCR analysis confirmed these results. Similar results were obtained analysing FRA-1 expression in fine needle aspiration biopsy (FNAB) samples. CONCLUSION: The data shown here suggest that FRA-1 expression, including its intracellular localization, may be considered a useful marker for hyperplastic and neoplastic proliferative breast disorders

Star Clusters in Pseudo-Bulges of Spiral Galaxies

We present a study of the properties of the star-cluster systems around pseudo-bulges of late-type spiral galaxies using a sample of 11 galaxies with distances from 17 to 37 Mpc. Star clusters are identified from multiband HST ACS and WFPC2 imaging data by combining detections in 3 bands (F435W and F814W with ACS and F606W with WFPC2). The photometric data are then compared to population synthesis models to infer the masses and ages of the star clusters. Photometric errors and completeness are estimated by means of artificial source Monte Carlo simulations. Dust extinction is estimated by considering F160W NICMOS observations of the central regions of the galaxies, augmenting our wavelength coverage. In all galaxies we identify star clusters with a wide range of ages, from young (age < 8 Myr) blue clusters, with typical mass of 10^3 Msun to older (age > 100-250 Myr), more massive, red clusters. Some of the latter might likely evolve into objects similar to the Milky Way's globular clusters. We compute the specific frequencies for the older clusters with respect to the galaxy and bulge luminosities. Specific frequencies relative to the galaxy light appear consistent with the globular cluster specific frequencies of early-type spirals. We compare the specific frequencies relative to the bulge light with the globular cluster specific frequencies of dwarf galaxies, which have a surface-brightness profile that is similar to that of the pseudo-bulges in our sample. The specific frequencies we derive for our sample galaxies are higher than those of the dwarf galaxies, supporting an evolutionary scenario in which some of the dwarf galaxies might be the remnants of harassed late-type spiral galaxies which hosted a pseudo-bulge.Comment: 34 pages, 9 figures, AJ accepte

Monitoring Winter and Summer Abundance of Cetaceans in the Pelagos Sanctuary (Northwestern Mediterranean Sea) Through Aerial Surveys

Systematic long-term monitoring of abundance is essential to inform conservation measures and evaluate their effectiveness. To instigate such work in the Pelagos Sanctuary in the Mediterranean, two aerial surveys were conducted in winter and summer 2009. A total of 467 (131 in winter, 336 in summer) sightings of 7 species was made. Sample sizes were sufficient to estimate abundance of fin whales in summer (148; 95% CI = 87–254) and striped dolphins in winter (19,462; 95% CI = 12 939–29 273) and in summer (38 488; 95% CI = 27 447–53 968). Numbers of animals within the Sanctuary are significantly higher in summer, when human activities and thus potential population level impacts are highest. Comparisons with data from past shipboard surveys suggest an appreciable decrease in fin whales within the Sanctuary area and an appreciable increase in striped dolphins. Aerial surveys proved to be more efficient than ship surveys, allowing more robust estimates, with smaller CIs and CVs. These results provide essential baseline data for this marine protected area and continued regular surveys will allow the effectiveness of the MPA in terms of cetacean conservation to be evaluated and inform future management measures. The collected data may also be crucial in assessing whether ship strikes, one of the main causes of death for fin whales in the Mediterranean, are affecting the Mediterranean population

Experimental Assessment of the Water Quality Influence on the Phosphorus Uptake of an Invasive Aquatic Plant: Biological Responses throughout Its Phenological Stage

International audienceUnderstanding how an invasive plant can colonize a large range of environments is still a great challenge in freshwater ecology. For the first time, we assessed the relative importance of four factors on the phosphorus uptake and growth of an invasive macrophyte Elodea nuttallii (Planch.) St. John. This study provided data on its phenotypic plasticity, which is frequently suggested as an important mechanism but remains poorly investigated. The phosphorus uptake of two Elodea nuttallii subpopulations was experimentally studied under contrasting environmental conditions. Plants were sampled in the Rhine floodplain and in the Northern Vosges mountains, and then maintained in aquaria in hard (Rhine) or soft (Vosges) water. Under these conditions, we tested the influence of two trophic states (eutrophic state, 100 mu g.l(-1) P-PO43- and hypertrophic state, 300 mu g.l(-1) P-PO43-) on the P metabolism of plant subpopulations collected at three seasons (winter, spring and summer). Elodea nuttallii was able to absorb high levels of phosphorus through its shoots and enhance its phosphorus uptake, continually, after an increase of the resource availability (hypertrophic > eutrophic). The lowest efficiency in nutrient use was observed in winter, whereas the highest was recorded in spring, what revealed thus a storage strategy which can be beneficial to new shoots. This experiment provided evidence that generally, the water trophic state is the main factor governing P uptake, and the mineral status (softwater > hardwater) of the stream water is the second main factor. The phenological stage appeared to be a confounding factor to P level in water. Nonetheless, phenology played a role in P turnover in the plant. Finally, phenotypic plasticity allows both subpopulations to adapt to a changing environment

Investigation of the direct effects of salmon calcitonin on human osteoarthritic chondrocytes

<p>Abstract</p> <p>Background</p> <p>Calcitonin has been demonstrated to have chondroprotective effects under pre-clinical settings. It is debated whether this effect is mediated through subchondral-bone, directly on cartilage or both in combination. We investigated possible direct effects of salmon calcitonin on proteoglycans and collagen-type-II synthesis in osteoarthritic (OA) cartilage.</p> <p>Methods</p> <p>Human OA cartilage explants were cultured with salmon calcitonin [100 pM-100 nM]. Direct effects of calcitonin on articular cartilage were evaluated by 1) measurement of proteoglycan synthesis by incorporation of radioactive labeled ³⁵SO₄[5 μCi] 2) quantification of collagen-type-II formation by pro-peptides of collagen type II (PIINP) ELISA, 3) QPCR expression of the calcitonin receptor in OA chondrocytes using four individual primer pairs, 4) activation of the cAMP signaling pathway by EIA and, 5) investigations of metabolic activity by AlamarBlue.</p> <p>Results</p> <p>QPCR analysis and subsequent sequencing confirmed expression of the calcitonin receptor in human chondrocytes. All doses of salmon calcitonin significantly elevated cAMP levels (P < 0.01 and P < 0.001). Calcitonin significantly and concentration-dependently [100 pM-100 nM] induced proteoglycan synthesis measured by radioactive ³⁵SO₄incorporation, with a 96% maximal induction at 10 nM (P < 0.001) corresponding to an 80% induction of 100 ng/ml IGF, (P < 0.05). In alignment with calcitonin treatments [100 pM-100 nM] resulted in 35% (P < 0.01) increased PIINP levels.</p> <p>Conclusion</p> <p>Calcitonin treatment increased proteoglycan and collagen synthesis in human OA cartilage. In addition to its well-established effect on subchondral bone, calcitonin may prove beneficial to the management of joint diseases through direct effects on chondrocytes.</p