ALS Yeast Models-Past Success Stories and New Opportunities

In the past two decades, yeast models have delivered profound insights into basic mechanisms of protein misfolding and the dysfunction of key cellular pathways associated with amyotrophic lateral sclerosis (ALS). Expressing ALS-associated proteins, such as superoxide dismutase (SOD1), TAR DNA binding protein 43 (TDP-43) and Fused in sarcoma (FUS), in yeast recapitulates major hallmarks of ALS pathology, including protein aggregation, mislocalization and cellular toxicity. Results from yeast have consistently been recapitulated in other model systems and even specimens from human patients, thus providing evidence for the power and validity of ALS yeast models. Focusing on impaired ribonucleic acid (RNA) metabolism and protein misfolding and their cytotoxic consequences in ALS, we summarize exemplary discoveries that originated from work in yeast. We also propose previously unexplored experimental strategies to modernize ALS yeast models, which will help to decipher the basic pathomechanisms underlying ALS and thus, possibly contribute to finding a cure

Corporate Social Performance and Non-Financial Reporting in the Cruise Industry: Paving the way towards UN Agenda 2030

This article investigates the role of accounting disciplines in assessing and fostering corporate social performance (CSP) of business organisations to meet the UN Agenda 2030. Drawing from legitimacy theory, this qualitative study analyses if and how non-financial reporting positively affects and fosters CSP practices and outcomes within the cruise industry. Specifically, using a case study methodology, a major cruise company has been analysed outlining its sustainable behaviour, through the manual content analysis of sustainability reports (2016–2017–2018), in achieving the sustainable development goals (SDGs) introduced by the UN Agenda 2030. The findings outline that the cruise company presents a clear sustainability and community orientation and adopts several initiatives, mostly focused on environmental and social issues, addressed to meet the 17 SDGs, where reputation and positive image have been recognised as the major antecedents in the perspective of CSP. This study contributes to the literature giving a broader and different reading of sustainability reporting as a “booster” of the CSP in meeting the SDGs and a further interesting application of the legitimacy theory, and offers managerial implications to systematise the content of non-financial reporting by improving the quality of disclosure to achieve the SDGs, with focus on CSP processes, outcomes and ways

Toxicological assays with Vicia faba L. for monitoring bioremediation processes of soils contaminated by polycyclic aromatic hydrocarbons and total petroleum hydrocarbons

The ecotoxicological assays are used both in the assessment of the toxicity of various matrices and to estimate the effectiveness of the remediation of a matrix over time. Several organisms can be employed in eco-toxicological tests in the lab, or can be observed in their natural environment representing indicators of specific environmental conditions. Higher plants can be exploited as good indicators of the quality of different matrices and used both as a standard during remediation processes of contaminated soil but also in the evaluation of soil quality. In fact, the only chemical characterization of a polluted soil does not allow to predict the real toxicity of the matrix, that may depend on the original pollutants, on degradations intermediates, often unknown, and on the synergic actions of the different toxicants. The aim of this research was to evaluate the efficacy of the process abatement of the initial concentration, in a contaminated soil, of polycyclic aromatic hydrocarbons (PAHs, 6480 ppm) and of total petroleum hydrocarbons (TPHs, 18347 ppm) by means of a biopile in terms of reduction of the final matrix toxicity. For this reason soil samples at different time intervals from the beginning of treatment in biopile were collected and chemically characterized, for the monitoring and assessment of the possible phyto-genotoxic effects on the model system Vicia faba L. The biopile treatment determined the depletion of PAHs and TPHs below 10 and 100 ppm respectively. Biological parameters such as germination and root elongation were also evaluated as well as the possible genotoxic effects by means of cytological analysis of mitotic behaviour of root meristem, based on detection of chromosomal aberrations in mitotic cells, and of micronuclei formation, detectable in interphase cells. As additionally genotoxicity endpoint, cytohistochemical detection of in situ DNA fragmentation in root apices was achieved by TUNEL reaction, that allows to recognize both single and double-strand breaks at cellular and tissue level. Comparing the obtained results it was found that the phyto-genotoxic effect of contaminated soil was completely depleted after treatment in biopile and Vicia faba was found to be a good approach for monitoring the biodegradation processes and the toxicity of these solid matrices at the end of a bio-based decontamination approach. Keywords: genotoxicity, phytotoxicity, polycyclic aromatic hydrocarbons, total petroleum hydrocarbon

Information networks and power: confronting the "wicked problem" of REDD+ in Indonesia

Reducing Emissions from Deforestation and Forest Degradation (REDD+) is a priority issue for forest and climate policy in Indonesia, and REDD+ policy-making activity has been characterized by considerable public consultation. Despite this engagement, discussions on REDD+ in Indonesia are reported to have remained top-down, a disconcerting pattern when adaptive governance and transformational change require cross-scale and cross-sectoral communication. Explicitly modeling the patterns of information exchange related to REDD+ can clarify these claims and help identify potential barriers to the transformational change needed to implement REDD+. We used data obtained through semistructured and structured interviews held in 2011 with representatives from a broad range of organizations (N = 64), formally or informally involved in the national REDD+ policy processes in Indonesia, to study REDD-related information exchange. Adopting a social network analysis approach, we found that (1) organizations perceived as most influential in REDD+ policy formulation, often, but not exclusively, those with institutional authority over particular aspects of REDD+, tend not to seek information from other actors and (2) organizations exchange information primarily within three clusters of similar organizations, with weak connections between clusters. This evidence suggests weak information exchange between the national government, national civil society, and transnational actors. We contend that the emergence of brokers able to connect these different clusters will be crucial for effective and inclusive REDD+ governance in Indonesia

On random flights with non-uniformly distributed directions

This paper deals with a new class of random flights $\underline{\bf X}_d(t),t>0,$ defined in the real space $\mathbb{R}^d, d\geq 2,$ characterized by non-uniform probability distributions on the multidimensional sphere. These random motions differ from similar models appeared in literature which take directions according to the uniform law. The family of angular probability distributions introduced in this paper depends on a parameter $\nu\geq 0$ which gives the level of drift of the motion. Furthermore, we assume that the number of changes of direction performed by the random flight is fixed. The time lengths between two consecutive changes of orientation have joint probability distribution given by a Dirichlet density function. The analysis of $\underline{\bf X}_d(t),t>0,$ is not an easy task, because it involves the calculation of integrals which are not always solvable. Therefore, we analyze the random flight $\underline{\bf X}_m^d(t),t>0,$ obtained as projection onto the lower spaces $\mathbb{R}^m,m<d,$ of the original random motion in $\mathbb{R}^d$. Then we get the probability distribution of $\underline{\bf X}_m^d(t),t>0.$ Although, in its general framework, the analysis of $\underline{\bf X}_d(t),t>0,$ is very complicated, for some values of $\nu$, we can provide some results on the process. Indeed, for $\nu=1$, we obtain the characteristic function of the random flight moving in $\mathbb{R}^d$. Furthermore, by inverting the characteristic function, we are able to give the analytic form (up to some constants) of the probability distribution of $\underline{\bf X}_d(t),t>0.$Comment: 28 pages, 3 figure

Integrating mitigation and adaptation in climate and land use policies in Brazil: a policy document analysis

This paper investigates climate policy integration and coherence in land use policies in Brazil. Unlike other policy analyses a key aim is to assess ‘internal policy coherence’ in the climate change domain, or the extent to which positive and negative interactions between mitigation and adaptation are taken into account in policy formulation. The paper is based on a systematic content analysis of major federal level climate change and land use policies. The results indicate a stronger focus on climate change mitigation compared to adaptation in all land uses. Integrated approaches that consider mutually supportive mitigation and adaptation actions are called for in key climate change policies, but so far such linkages remain largely unexplored in sectoral policies. While some progress in this regard occurred in the agricultural sector, this has not translated into actual policy actions that are of use to small-scale producers. In the forest domain the focus remains almost exclusively on climate change mitigation. Three main recommendations are drawn. First, more knowledge about locally specific climate change impacts, resilience, capacity and measures for climate change adaptation is needed in order to increase the opportunities to pursue mutually beneficial approaches to climate change mitigation, adaptation and development, in particular in the forestry sector. Second, policy makers need to address more explicitly potential trade-offs between mitigation and adaptation in both policy formulation and implementation. Third, policy action on how to achieve mutually beneficial outcomes and avoid trade-offs between mitigation and adaptation needs to be operationalised into concrete policy objectives within sectoral policies and into practices that apply not just to agriculture and livestock systems, but to forested landscapes as well

Transfer of chemical elements from milk to dairy products

Abstract A pilot study was carried out to evaluate the transfer of As, Cd, Pb, Ni, Cr and Hg from milk to dairy products and to correlate their behavior with that of some components and macro-elements of milk. Due to the low level of these chemical elements in most common commercial products, hard cheese was produced starting from cow's milk spiked with the analytes of interest. Several intermediate and final products coming from cheese making were sampled and analysed for content of fat, dry matter, proteins, macro and oligo-elements. The relationship between spiked elements and milk components was evaluated through both the study of concentration factors and the statistical analysis (Principal Component Analysis and correlation matrix). Except for As and Hg, a clear correlation between spiked elements and milk components was found so a likely bond with proteins, fat and dry matter was demonstrated. As for Pb, for which a legal limit (Maximum Level, ML) is set in the pertinent European regulations, it was found that the link with proteins could lead to an increase of this element concentration different from the mere concentration factor from milk to cheese. Furthermore, it was proven that the stage of ripening affected the variation of Pb concentration so this aspect should be deeply considered in case of setting a ML in cheese or extrapolating a ML from milk to cheese

Water Crisis In São Paulo Evaluated Under The Disaster's Point Of View

Brazil's Southeast region has been facing water supply challenges, especially concerning the metropolitan region of São Paulo, since 2014. We adressed the water crisis from the disaster's point of view, considering that one of the guiding features to characterize a disaster is the amount of affected people and the losses associated. In the situation under review, there are people directly affected, because of intermittent house water supply, and indirectly affected by the increase of prices for goods, since their production and manufacturing depend on the availability of water. We presented each of the stages of a disaster risk management: prevention, mitigation, preparedness, response and recovery. The risk of water scarcity in São Paulo is only one aspect of the discussion involving the effectiveness of management processes and the need for investments in the area, especially in reducing wasteful distribution.191214

Equity and REDD+ in the media: a comparative analysis of policy discourses

Reducing emissions from deforestation and forest degradation (REDD+) is primarily a market-based mechanism for achieving the effective reduction of carbon emissions from forests. Increasingly, however, concerns are being raised about the implications of REDD+ for equity, including the importance of equity for achieving effective carbon emission reductions from forests. Equity is a multifaceted concept that is understood differently by different actors and at different scales, and public discourse helps determine which equity concerns reach the national policy agenda. Results from a comparative media analysis of REDD+ public discourse in four countries show that policy makers focus more on international than national equity concerns, and that they neglect both the need for increased participation in decision making and recognition of local and indigenous rights. To move from addressing the symptoms to addressing the causes of inequality in REDD+, policy actors need to address issues related to contextual equity, that is, the social and political root causes of inequality

A comparison between the β-globin gene clusters of domestic sheep (Ovis aries) and Sardinian mouflon (Ovis gmelini musimon)

International audienc