EMP2 regulates angiogenesis in endometrial cancer cells through induction of VEGF.

Understanding tumor-induced angiogenesis is a challenging problem with important consequences for the diagnosis and treatment of cancer. In this study, we define a novel function for epithelial membrane protein-2 (EMP2) in the control of angiogenesis. EMP2 functions as an oncogene in endometrial cancer, and its expression has been linked to decreased survival. Using endometrial cancer xenografts, modulation of EMP2 expression resulted in profound changes to the tumor microvasculature. Under hypoxic conditions, upregulation of EMP2 promoted vascular endothelial growth factors (VEGF) expression through a HIF-1α-dependent pathway and resulted in successful capillary-like tube formation. In contrast, reduction of EMP2 correlated with reduced HIF-1α and VEGF expression with the net consequence of poorly vascularized tumors in vivo. We have previously shown that targeting of EMP2 using diabodies in endometrial cancer resulted in a reduction of tumor load, and since then we have constructed a fully human EMP2 IgG1. Treatment of endometrial cancer cells with EMP2-IgG1 reduced tumor load with a significant improvement in survival. These results support the role of EMP2 in the control of the tumor microenvironment and confirm the cytotoxic effects observed by EMP2 treatment in vivo

Emergence of metronidazole-resistant Bacteroides fragilis, India.

This article does not have an abstract

Neurotization Improves Contractile Forces of Tissue-Engineered Skeletal Muscle

Engineered functional skeletal muscle would be beneficial in reconstructive surgery. Our previous work successfully generated 3-dimensional vascularized skeletal muscle in vivo. Because neural signals direct muscle maturation, we hypothesized that neurotization of these constructs would increase their contractile force. Additionally, should neuromuscular junctions (NMJs) develop, indirect stimulation (via the nerve) would be possible, allowing for directed control. Rat myoblasts were cultured, suspended in fibrin gel, and implanted within silicone chambers around the femoral vessels and transected femoral nerve of syngeneic rats for 4 weeks. Neurotized constructs generated contractile forces 5 times as high as the non-neurotized controls. Indirect stimulation via the nerve elicited contractions of neurotized constructs. Curare administration ceased contraction in these constructs, providing physiologic evidence of NMJ formation. Histology demonstrated intact muscle fibers, and immunostaining positively identified NMJs. These results indicate that neurotization of engineered skeletal muscle significantly increases force generation and causes NMJs to develop, allowing indirect muscle stimulation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/63133/1/ten.2007.0003.pd

Digitally-enhanced lubricant evaluation scheme for hot stamping applications

Digitally-enhanced technologies are set to transform every aspect of manufacturing. Networks of sensors that compute at the edge (streamlining information flow from devices and providing real-time local data analysis), and emerging Cloud Finite Element Analysis technologies yield data at unprecedented scales, both in terms of volume and precision, providing information on complex processes and systems that had previously been impractical. Cloud Finite Element Analysis technologies enable proactive data collection in a supply chain of, for example the metal forming industry, throughout the life cycle of a product or process, which presents revolutionary opportunities for the development and evaluation of digitally-enhanced lubricants, which requires a coherent research agenda involving the merging of tribological knowledge, manufacturing and data science. In the present study, data obtained from a vast number of experimentally verified finite element simulation results is used for a metal forming process to develop a digitally-enhanced lubricant evaluation approach, by precisely representing the tribological boundary conditions at the workpiece/tooling interface, i.e., complex loading conditions of contact pressures, sliding speeds and temperatures. The presented approach combines the implementation of digital characteristics of the target forming process, data-guided lubricant testing and mechanism-based accurate theoretical modelling, enabling the development of data-centric lubricant limit diagrams and intuitive and quantitative evaluation of the lubricant performance

Tiny scale opacity fluctuations from VLBA, MERLIN and VLA observations of HI absorption toward 3C 138

The structure function of opacity fluctuations is a useful statistical tool to study tiny scale structures of neutral hydrogen. Here we present high resolution observation of HI absorption towards 3C 138, and estimate the structure function of opacity fluctuations from the combined VLA, MERLIN and VLBA data. The angular scales probed in this work are ~ 10-200 milliarcsec (about 5-100 AU). The structure function in this range is found to be well represented by a power law S_tau(x) ~ x^{beta} with index beta ~ 0.33 +/- 0.07 corresponding to a power spectrum P_tau(U) ~ U^{-2.33}. This is slightly shallower than the earlier reported power law index of ~ 2.5-3.0 at ~ 1000 AU to few pc scales. The amplitude of the derived structure function is a factor of ~ 20-60 times higher than the extrapolated amplitude from observation of Cas A at larger scales. On the other hand, extrapolating the AU scale structure function for 3C 138 predicts the observed structure function for Cas A at the pc scale correctly. These results clearly establish that the atomic gas has significantly more structures in AU scales than expected from earlier pc scale observations. Some plausible reasons are identified and discussed here to explain these results. The observational evidence of a shallower slope and the presence of rich small scale structures may have implications for the current understanding of the interstellar turbulence.Comment: 6 pages, 5 figures. Accepted for publication in ApJ. The definitive version will be available at http://iopscience.iop.org

Muonic hydrogen cascade time and lifetime of the short-lived 2S2S state

Metastable ${2S}$ muonic-hydrogen atoms undergo collisional ${2S}$-quenching, with rates which depend strongly on whether the $\mu p$ kinetic energy is above or below the ${2S}\to {2P}$ energy threshold. Above threshold, collisional ${2S} \to {2P}$ excitation followed by fast radiative ${2P} \to {1S}$ deexcitation is allowed. The corresponding short-lived $\mu p ({2S})$ component was measured at 0.6 hPa $\mathrm{H}_2$ room temperature gas pressure, with lifetime $\tau_{2S}^\mathrm{short} = 165 ^{+38}_{-29}$ ns (i.e., $\lambda_{2S}^\mathrm{quench} = 7.9 ^{+1.8}_{-1.6} \times 10^{12} \mathrm{s}^{-1}$ at liquid-hydrogen density) and population $\epsilon_{2S}^\mathrm{short} = 1.70^{+0.80}_{-0.56}$ % (per $\mu p$ atom). In addition, a value of the $\mu p$ cascade time, $T_\mathrm{cas}^{\mu p} = (37\pm5)$ ns, was found.Comment: 4 pages, 3 figure

Isolated radial head dislocation, a rare and easily missed injury in the presence of major distracting injuries: a case report

High velocity accidents can lead to major injuries – long bone fractures, abdominal trauma, pelvic fractures and chest injuries. These injuries can act as distracting factors during the initial assessment of a polytrauma patient and innocuous but significant smaller injuries can be missed. We present a rare case of isolated anterolateral radial head dislocation in a polytrauma patient

Suzaku Observations of the Black Hole H1743-322 in Outburst

We observed the Galactic black hole candidate H1743-322 with Suzaku for approximately 32 ksec, while the source was in a low/hard state during its 2008 outburst. We collected and analyzed the data with the HXD/PIN, HXD/GSO and XIS cameras spanning the energy range from 0.7-200 keV. Fits to the spectra with simple models fail to detect narrow Fe XXV and Fe XXVI absorption lines, with 90% confidence upper limits of 3.5 eV and 2.5 eV on the equivalent width, respectively. These limits are commensurate with those in the very high state, but are well below the equivalent widths of lines detected in the high/soft state, suggesting that disk winds are partially state-dependent. We discuss these results in the context of previous detections of ionized Fe absorption lines in H1743-322 and connections to winds and jets in accreting systems. Additionally, we report the possible detection of disk reflection features, including an Fe K emission line.Comment: 16 pages, 4 figures, 4 tables. Accepted for publication in ApJ