Sialic acid specific lectins from Episesarma tetragonum (Decapoda, Grapsidae): isolation, purification and characterization

Two sialic acid specific lectins Episesarma tetragonum agglutinin–1 and 2 were purified from the hemolymph of the Mangrove crab, Episesarma tetragonum. The major lectin was purified using CNBr-activated sepharose 4B conjugated to fetuin. N-acetyl glucosamine containing buffer was used for elution. The hemagglutination activity of purified lectin was inhibited by glycoproteins containing Siaα, 2-3Galβ, 1-4 GlcNAc linkages. On SDS-PAGE, the molecular weight of calcium dependent lectin was observed to be 70 kDa. Lectin had the maximum activity at a wide range of pH (6.5 – 9.5) and temperature (0 - 40 °C).  The physicochemical characteristics of the minor agglutinin showed that its hemagglutinating activity was calcium dependent, optimum at pH 8 – 9.5 and temperature 0 – 37 °C. The only potent inhibitor of minor lectin was bovine submaxillary mucin. An attempt was also made to purify minor lectin by affinity chromatography using bovine submaxillary mucin coupled to CNBr-activated sepharose 4B column. The lectin was eluted with elution buffer containing ethylene diamine tetra acetate. Strong inhibition of purified minor lectin by bovine submaxillary mucin and non-inhibitory action of de-O-acetylated bovine submaxillary mucin suggested that the lectin was O-acetyl sialic acid specific

Cor triloculare biventriculare with left superior vena cava

Cor triloculare biventriculare is a rare congenital malformation of the heart in which there is a complete absence of the atrial septum. It is usually associated with other anomalies like complete atrioventricular canal defect, polysplenic syndrome, isolated dextrocardia, Ellis-van Creveld syndrome, or persistent left superior vena cava. We report a case of a stillborn male foetus of 35 weeks gestation with common atrium, complete atrioventricular canal defect, and persistent left superior vena cava. The possible embryological basis and clinical implication of this variation are discussed. (Folia Morphol 2011; 70, 2: 135–138

PENGARUH UMUR PANEN RIMPANG TERHADAP PERUBAHAN FISIOLOGI DAN VIABILITAS BENIH JAHE PUTIH BESAR SELAMA PENYIMPANAN

ABSTRAKSalah satu faktor yang menentukan daya simpan benih jahe putihbesar (JPB) adalah mutu. Mutu benih sangat ditentukan oleh tingkatkemasakan rimpang. Penelitian bertujuan untuk mengetahui pengaruhumur panen terhadap perubahan fisiologi dan viabilitas benih selamapenyimpanan. Penelitian dilaksanakan di Rumah Kaca dan LaboratoriumTeknologi Benih, Balai Penelitian Tanaman Rempah dan Obat sertaLaboratorium Pascapanen IPB Bogor, mulai bulan Juli 2012 sampaidengan Februari 2013. Rancangan yang digunakan adalah acak lengkap(RAL) dengan lima ulangan. Perlakuan yang diuji adalah tiga tingkat umurpanen benih 7, 8, dan 9 bulan setelah tanam (BST). Pengamatan dilakukanterhadap perubahan fisiologis (penyusutan bobot, persentase rimpangbertunas, tunas, kadar air, dan laju respirasi), serta viabilitas rimpang benih(daya tumbuh, tinggi, dan bobot kering bibit). Hasil penelitianmenunjukkan rimpang benih umur 7 dan 8 BST mempunyai daya simpanterbaik karena menghasilkan masing-masing total angka penyusutan bobotlebih rendah (24,65 dan 25,25%) dan tunas lebih pendek (0,30 dan 1,08cm) dibandingkan dengan umur panen 9 BST (27,13% dan 1,62 cm),selama 4 bulan disimpan. Masa dormansi rimpang benih JPB mulai pecahsetelah mengalami periode simpan 2 bulan. Pertumbuhannya mulaiseragam setelah 3 bulan simpan. Umur panen jahe 7 dan 8 BSTmempunyai derajat dormansi yang lebih tinggi dibanding 9 BST. Rimpangbenih umur panen 7, 8, dan 9 BST mempunyai daya tumbuh tinggi (>95%)dan pertumbuhan bibit seragam setelah 3 bulan disimpan.Kata kunci: Zingiber officinale Rosc., penyimpanan, benih, perubahanfisiologis, viabilitasABSTRACTOne of the factors that determine the storability of seed rhizome ofwhite big ginger (WBG) is quality. The quality is determined by thematurity levels of seed rhizome. The aim of the experiment was to observethe effect of harvesting time on physiological changes and seed viability ofWBG seed rhizomes during the storage. The experiment was conducted atGreen House and Seed Technology Laboratory of Indonesian Spice andMedicinal Crops Research Institute, Bogor and Postharvest Laboratory,IPB, from July 2012 to February 2013. The experiment was arranged in acompletely randomized design with five replications. The treatmentstested were three levels of WBG seed rhizome harvesting time: 7, 8, and 9month after planting (MAP). Variables observed were physiologicalchanges of seed rhizomes during the storage (weight loss, sproutingpercentage, shoot height, respiration rate, and moisture content) andviability (growth ability, height, and dry weight of the seedling). Theresults showed that seed rhizomes at 7 and 8 had the best storability, sinceit was produced each low rate of weight loss (24.65 and 25.25%), andshoots shorter (0.3 and 1.08 cm) than 9 MAP (27.13% and 1.62 cm), for 4months in storage. Dormancy of WBG seed rhizomes has been brokenafter 2 months in storage. Harvesting at 7 and 8 showed a degree ofdormancy higher than the harvesting age 9 MAP. Harvesting time at 7, 8,and 9 MAP had high growth ability (> 95%) and uniform seedling growthafter 3 months in storage.Keywords: Zingiber officinale Rosc., storage, seed, physiologicalchanges, viabilit

Construction status and prospects of the Hyper-Kamiokande project

The Hyper-Kamiokande project is a 258-kton Water Cherenkov together with a 1.3-MW high-intensity neutrino beam from the Japan Proton Accelerator Research Complex (J-PARC). The inner detector with 186-kton fiducial volume is viewed by 20-inch photomultiplier tubes (PMTs) and multi-PMT modules, and thereby provides state-of-the-art of Cherenkov ring reconstruction with thresholds in the range of few MeVs. The project is expected to lead to precision neutrino oscillation studies, especially neutrino CP violation, nucleon decay searches, and low energy neutrino astronomy. In 2020, the project was officially approved and construction of the far detector was started at Kamioka. In 2021, the excavation of the access tunnel and initial mass production of the newly developed 20-inch PMTs was also started. In this paper, we present a basic overview of the project and the latest updates on the construction status of the project, which is expected to commence operation in 2027

Prospects for neutrino astrophysics with Hyper-Kamiokande

Hyper-Kamiokande is a multi-purpose next generation neutrino experiment. The detector is a two-layered cylindrical shape ultra-pure water tank, with its height of 64 m and diameter of 71 m. The inner detector will be surrounded by tens of thousands of twenty-inch photosensors and multi-PMT modules to detect water Cherenkov radiation due to the charged particles and provide our fiducial volume of 188 kt. This detection technique is established by Kamiokande and Super-Kamiokande. As the successor of these experiments, Hyper-K will be located deep underground, 600 m below Mt. Tochibora at Kamioka in Japan to reduce cosmic-ray backgrounds. Besides our physics program with accelerator neutrino, atmospheric neutrino and proton decay, neutrino astrophysics is an important research topic for Hyper-K. With its fruitful physics research programs, Hyper-K will play a critical role in the next neutrino physics frontier. It will also provide important information via astrophysical neutrino measurements, i.e., solar neutrino, supernova burst neutrinos and supernova relic neutrino. Here, we will discuss the physics potential of Hyper-K neutrino astrophysics

Highly-parallelized simulation of a pixelated LArTPC on a GPU

The rapid development of general-purpose computing on graphics processing units (GPGPU) is allowing the implementation of highly-parallelized Monte Carlo simulation chains for particle physics experiments. This technique is particularly suitable for the simulation of a pixelated charge readout for time projection chambers, given the large number of channels that this technology employs. Here we present the first implementation of a full microphysical simulator of a liquid argon time projection chamber (LArTPC) equipped with light readout and pixelated charge readout, developed for the DUNE Near Detector. The software is implemented with an end-to-end set of GPU-optimized algorithms. The algorithms have been written in Python and translated into CUDA kernels using Numba, a just-in-time compiler for a subset of Python and NumPy instructions. The GPU implementation achieves a speed up of four orders of magnitude compared with the equivalent CPU version. The simulation of the current induced on 10^3 pixels takes around 1 ms on the GPU, compared with approximately 10 s on the CPU. The results of the simulation are compared against data from a pixel-readout LArTPC prototype

Women's attire and costumes in the Perungathai

Tamil literature is the epitome of the great ideas of our ancient Tamils. Literature is a country's time glass. In which people can connect with them in order to progress. The societal changes in a country's artistic environment are reflected in the local literature. The Epic literature is to highlight the importance of women in their clothing and ornaments. The women’s in the Perungathai are the beautiful women who come before our eyes. The prosperity of a country is determined by the women living in the country. Women’s who lived happily with dresses, ornaments and Savor flowers. Therefore, the dress and costume of the female protagonists in Perungathai are very special and imitated by the present generation