Effects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutants

Membrane-related bioeffects have been reported in response to both radio-frequency (RF) and extremely low-frequency (ELF) electromagnetic fields (EMFs), particularly in neural cells. We have tested whether RF fields might cause inner membrane leakage in ML35 E. coli cells, which express β-galactosidase (lacZ) constitutively, but lack the lacY permease required for substrate entry. The activity of lacZ (indicating substrate leakage through the inner cell membrane) was increased only slightly by RF exposure (1 GHz, 0.5 W) over 45 min. Since lacZ activity showed no further increase with a longer exposure time of 90 min, this suggests that membrane permeability per se is not significantly affected by RF fields, and that slight heating (≤ 0.1°C) could account for this small difference. Temperature-sensitive (ts) mutants of the nematode, Caenorhabditis elegans, are wild-type at 15°C but develop the mutant phenotype at 25°C; an intermediate temperature of 21°C results in a reproducible mixture of both phenotypes. For two ts mutants affecting transmembrane receptors (TRA-2 and GLP-1), RF exposure for 24 h during the thermocritical phase strongly shifts the phenotype mix at 21°C towards the mutant end of the spectrum. For ts mutants affecting nuclear proteins, such phenotype shifts appear smaller (PHA-1) or non-significant (LIN-39), apparently confirming suggestions that RF power is dissipated mainly in the plasma membrane of cells. However, these phenotype shifts are no longer seen when microwave treatment is applied at 21°C in a modified exposure apparatus that minimises the temperature difference between sham and exposed conditions. Like other biological effects attributed to microwaves in the C. elegans system, phenotype shifts in ts mutants appear to be an artefact caused by very slight heating

Millennial-scale climatic variability between 340000 and 270000 years ago in SW Europe : evidence from a NW Iberian margin pollen sequence

© 2009 The Authors. This article is distributed under the terms of the Creative Commons Attribution 3.0 License. The definitive version was published in Climate of the Past 5 (2009): 53-72, doi:10.5194/cp-5-53-2009We present a new high-resolution marine pollen record from NW Iberian margin sediments (core MD03-2697) covering the interval between 340 000 and 270 000 years ago, a time period centred on Marine Isotope Stage (MIS) 9 and characterized by particular baseline climate states. This study enables the documentation of vegetation changes in the north-western Iberian Peninsula and therefore the terrestrial climatic variability at orbital and in particular at millennial scales during MIS 9, directly on a marine stratigraphy. Suborbital vegetation changes in NW Iberia in response to cool/cold events are detected throughout the studied interval even during MIS 9e ice volume minimum. However, they appear more frequent and of higher amplitude during the 30 000 years following the MIS 9e interglacial period and during the MIS 9a-8 transition, which correspond to intervals of an intermediate to high ice volume and mainly periods of ice growth. Each suborbital cold event detected in NW Iberia has a counterpart in the Southern Iberian margin SST record. High to moderate amplitude cold episodes detected on land and in the ocean appear to be related to changes in deep water circulation and probably to iceberg discharges at least during MIS 9d, the mid-MIS 9c cold event and MIS 9b. This work provides therefore additional evidence of pervasive millennial-scale climatic variability in the North Atlantic borderlands throughout past climatic cycles of the Late Pleistocene, regardless of glacial state. However, ice volume might have an indirect influence on the amplitude of the millennial climatic changes in Southern Europe.This research was supported by IPEV (Institut Paul Emile Victor), PNEDC (Programme National d’Etude de la Dynamique du Climat), the Gary Comer Science and Education Foundation and the US National Science Foundation (OCE grants 8-4911100 and 8-256500)

European climate optimum and enhanced Greenland melt during the Last Interglacial

The Last Interglacial climatic optimum, ca. 128 ka, is the most recent climate interval signifi cantly warmer than present, providing an analogue (albeit imperfect) for ongoing global warming and the effects of Greenland Ice Sheet (GIS) melting on climate over the coming millennium. While some climate models predict an Atlantic meridional overturning circulation (AMOC) strengthening in response to GIS melting, others simulate weakening, leading to cooling in Europe. Here, we present evidence from new proxy-based paleoclimate and ocean circulation reconstructions that show that the strongest warming in western Europe coincided with maximum GIS meltwater runoff and a weaker AMOC early in the Last Interglacial. By performing a series of climate model sensitivity experiments, including enhanced GIS melting, we were able to simulate this confi guration of the Last Interglacial climate system and infer information on AMOC slowdown and related climate effects. These experiments suggest that GIS melt inhibited deep convection off the southern coast of Greenland, cooling local climate and reducing AMOC by ~24% of its present strength. However, GIS melt did not perturb overturning in the Nordic Seas, leaving heat transport to, and thereby temperatures in, Europe unaffected. © 2012 Geological Society of America