193 research outputs found
Hospital Discharge: Results From an Italian Multicenter Prospective Study Using Blaylock Risk Assessment Screening Score
PURPOSE:To analyze the predictive validity and reliability of the Blaylock Risk Assessment Screening Score (BRASS) Index in a large group of patients.
METHODS: Prospective multicenter observational study was conducted in six Italian hospitals. Data were collected in three phases.
FINDINGS: Seven hundred eleven patients were recruited. The mean length of hospitalization for low-risk patients was significantly shorter than those in the medium and high-risk groups. Patients with a BRASS Index lower than 10, unlike those with a higher BRASS Index, were mainly discharged home.
CONCLUSIONS: Our results indicate that the BRASS Index is useful to identify patients at risk for prolonged hospitalization.
CLINICAL RELEVANCE: The use of a validated BRASS instrument can be useful to screen the patients, improving individual discharge planning
Thio-linked UDP-peptide conjugates as O-GlcNAc transferase inhibitors
O-GlcNAc
transferase (OGT) is an essential glycosyltransferase
that installs the O-GlcNAc post-translational modification on the
nucleocytoplasmic proteome. We report the development of S-linked
UDPâpeptide conjugates as potent bisubstrate OGT inhibitors.
These compounds were assembled in a modular fashion by photoinitiated
thiolâene conjugation of allyl-UDP and optimal acceptor peptides
in which the acceptor serine was replaced with cysteine. The conjugate
VTPVCÂ(S-propyl-UDP)ÂTA (<i>K</i><sub>i</sub> = 1.3 ÎźM)
inhibits the OGT activity in HeLa cell lysates. Linear fusions of
this conjugate with cell penetrating peptides were explored as prototypes
of cell-penetrant OGT inhibitors. A crystal structure of human OGT
with the inhibitor revealed mimicry of the interactions seen in the
pseudo-Michaelis complex. Furthermore, a fluorophore-tagged derivative
of the inhibitor works as a high affinity probe in a fluorescence
polarimetry hOGT assay
Gold nanoparticles delivery in mammalian live cells: a critical review
Functional nanomaterials have recently attracted strong interest from the biology community, not only as potential drug delivery vehicles or diagnostic tools, but also as optical nanomaterials. This is illustrated by the explosion of publications in the field with more than 2,000 publications in the last 2 years (4,000 papers since 2000; from ISI Web of Knowledge, ânanoparticle and cellâ hit). Such a publication boom in this novel interdisciplinary field has resulted in papers of unequal standard, partly because it is challenging to assemble the required expertise in chemistry, physics, and biology in a single team. As an extreme example, several papers published in physical chemistry journals claim intracellular delivery of nanoparticles, but show pictures of cells that are, to the expert biologist, evidently dead (and therefore permeable). To attain proper cellular applications using nanomaterials, it is critical not only to achieve efficient delivery in healthy cells, but also to control the intracellular availability and the fate of the nanomaterial. This is still an open challenge that will only be met by innovative delivery methods combined with rigorous and quantitative characterization of the uptake and the fate of the nanoparticles. This review mainly focuses on gold nanoparticles and discusses the various approaches to nanoparticle delivery, including surface chemical modifications and several methods used to facilitate cellular uptake and endosomal escape. We will also review the main detection methods and how their optimum use can inform about intracellular localization, efficiency of delivery, and integrity of the surface capping
Report of the 12th Liaison Meeting
The 12th Liaison meeting was held in Brussels on 8th and 9th October 2015 to address the following Terms
of Reference:
TOR 1. Discussion on possible follow-Âââup to the main outputs/recommendations of:
⢠The 2015 RCMs -Âââ specific recommendations addressed to the Liaison Meeting
⢠PGECON, PGDATA, PGMed â outcomes and recommendations from their 2015 meeting
⢠STECF EWG and STECF Plenary -Âââ outcomes and recommendations from their 2015 meetings
⢠Data end users (ICES, STECF, RFMOs â GFCM, IATTC, ICCAT, IOTC, WCPFC, NAFO,
SPRFMO, CECAF, WECAFC)
TOR2. End user feedback on data transmission and related issues
⢠Discuss feedback received from data end-Âââusers on data transmission: main issues and possible
harmonization of end user feedback to the Commission
⢠JRC data transmission IT platform: experience gained and future steps
⢠Discuss best practices on automatization of data upload by MS: data validation tools used by
end users
⢠Discussion on new set-Âââup for STECF evaluation of AR2014 & data transmission 2014 used in
2015 â continue like this next year?
⢠Harmonisation and dissemination of DCF metadata: codelists, metiers, nomenclatures, best
practices, standards
⢠RCM data calls â overview of how MS responded
TOR 3. Regional cooperation
⢠Call for proposals MARE/2014/19 'ʚStrengthening Regional Cooperation in the area of fisheries
data collectionâ state of play'Ęš. Presentation by a representative of the two RCG grants and
discussions by LM thereafter. What should be the way forward?
⢠Regional databases
⢠Overview of use of the Regional Databases for RCMs in 2015 and problems identified
⢠Other developments (RDB trainings in 2015, RDB Med&BS development)
⢠Changes for the future â any recommendations from the LM?
⢠Future role of RCMs and DCF-Ââârelated meetings: best practices, coordination, cohesion and
common structure in line with emerging needs of DCF
TOR 4. EU MAP
⢠Discuss recommendations/ output of RCMs: List of proposed stocks, landing obligation, metiers
⢠Discuss design-Âââbased sampling in relation to DCF: does it fulfil DCF requirements?
TOR 5. Availability of data
⢠Overview of latest developments (DCF Database Feasibility Study and plans for a follow-Âââup
study to this)
TOR 6. AOB
⢠Agree on a list of recommendations relating to DCF (that MS will need to report on in their
AR2015) â COM will provide a compilation of proposed recommendations from LM & STECF
Plenaries in 2014 as input
⢠Prepare a list of recommended meetings for 2016 as guidance for MS
⢠Review and prioritize DCF-Ââârelated study proposals from RCMs, PGECON, EGs etc
⢠ICES update on workshop on concurrent sampling and plans to re-Âââevaluate survey
Combinatorial targeting and discovery of ligand-receptors in organelles of mammalian cells
Phage display screening allows the study of functional proteinâprotein interactions at the cell surface, but investigating intracellular organelles remains a challenge. Here we introduce internalizing-phage libraries to identify clones that enter mammalian cells through a receptor-independent mechanism and target-specific organelles as a tool to select ligand peptides and identify their intracellular receptors. We demonstrate that penetratin, an antennapedia-derived peptide, can be displayed on the phage envelope and mediate receptor-independent uptake of internalizing phage into cells. We also show that an internalizing-phage construct displaying an established mitochondria-specific localization signal targets mitochondria, and that an internalizing-phage random peptide library selects for peptide motifs that localize to different intracellular compartments. As a proof-of-concept, we demonstrate that one such peptide, if chemically fused to penetratin, is internalized receptor-independently, localizes to mitochondria, and promotes cell death. This combinatorial platform technology has potential applications in cell biology and drug development
Impact of power ultrasound on chemical and physicochemical quality indicators of strawberries dried by convection
[EN] A study on the quality parameters of strawberries dehydrated by convection assisted by power ultrasound
(US) at 40 70 C and 30 and 60W has been carried out for the first time. In general, the quality
of US-treated samples was higher than that of commercial samples. Even under the most severe conditions
used (US at 70 C and 60 W), high values of vitamin C retention (>65%) and scarce advance of Maillard
reaction (2-furoylmethyl derivatives of Lys and Arg < 90 mg 100 g 1 protein) were observed.
Rehydration ratio was not affected by the power applied and the obtained values were similar to those
of convectively-treated samples. According to the results here presented, US is a suitable example of
an emerging and environmentally friendly technology that accelerates convective drying, allowing the
obtainment of dried strawberries with premium quality.This work has been funded by Ministry of Economy and Competitiveness of Spain and ERDF (FEDER) (Project DPI2012-37466-C03-03), Fun-c-Food (CSD2007-00063 Consolider-INGENIO 2010), CYTED IBEROFUN (P109AC0302) and Comunidad de Valencia, Project PROMETEO/2010/062. J.G.S. also thanks CSIC and the EU for a predoctoral JAE grant. A.C.S. thanks the Spanish Ministry of Economy and Competitiveness for a Ramon y Cajal contract.Gamboa-Santos, J.; Montilla, A.; Cortijo Soria, A.; CĂĄrcel CarriĂłn, JA.; GarcĂa PĂŠrez, JV.; Villamiel, M. (2014). Impact of power ultrasound on chemical and physicochemical quality indicators of strawberries dried by convection. Food Chemistry. 161:40-46. https://doi.org/10.1016/j.foodchem.2014.03.106S404616
Metal Hydrides Form Halogen Bonds: Measurement of Energetics of Binding
The formation of halogen bonds from iodopentafluorobenzene and 1-iodoperfluorohexane to a series of bis(Ρ5-cyclopentadienyl)metal hydrides (Cp2TaH3, 1; Cp2MH2, M = Mo, 2, M = W, 3; Cp2ReH, 4; Cp2Ta(H)CO, 5; Cp = Ρ5-cyclopentadienyl) is demonstrated by 1H NMR spectroscopy. Interaction enthalpies and entropies for complex 1 with C6F5I and C6F13I are reported (ÎH° = â10.9 Âą 0.4 and â11.8 Âą 0.3 kJ/mol; ÎS° = â38 Âą 2 and â34 Âą 2 J/(mol¡K), respectively) and found to be stronger than those for 1 with the hydrogen-bond donor indole (ÎH° = â7.3 Âą 0.1 kJ/mol, ÎS° = â24 Âą 1 J/(mol¡K)). For the more reactive complexes 2â5, measurements are limited to determination of their low-temperature (212 K) association constants with C6F5I as 2.9 Âą 0.2, 2.5 Âą 0.1, <1.5, and 12.5 Âą 0.3 Mâ1, respectively
Report of the 12th Liaison Meeting
The 12th Liaison meeting was held in Brussels on 8th and 9th October 2015 to address the following Terms
of Reference:
TOR 1. Discussion on possible follow-Âââup to the main outputs/recommendations of:
⢠The 2015 RCMs -Âââ specific recommendations addressed to the Liaison Meeting
⢠PGECON, PGDATA, PGMed â outcomes and recommendations from their 2015 meeting
⢠STECF EWG and STECF Plenary -Âââ outcomes and recommendations from their 2015 meetings
⢠Data end users (ICES, STECF, RFMOs â GFCM, IATTC, ICCAT, IOTC, WCPFC, NAFO,
SPRFMO, CECAF, WECAFC)
TOR2. End user feedback on data transmission and related issues
⢠Discuss feedback received from data end-Âââusers on data transmission: main issues and possible
harmonization of end user feedback to the Commission
⢠JRC data transmission IT platform: experience gained and future steps
⢠Discuss best practices on automatization of data upload by MS: data validation tools used by
end users
⢠Discussion on new set-Âââup for STECF evaluation of AR2014 & data transmission 2014 used in
2015 â continue like this next year?
⢠Harmonisation and dissemination of DCF metadata: codelists, metiers, nomenclatures, best
practices, standards
⢠RCM data calls â overview of how MS responded
TOR 3. Regional cooperation
⢠Call for proposals MARE/2014/19 'ʚStrengthening Regional Cooperation in the area of fisheries
data collectionâ state of play'Ęš. Presentation by a representative of the two RCG grants and
discussions by LM thereafter. What should be the way forward?
⢠Regional databases
⢠Overview of use of the Regional Databases for RCMs in 2015 and problems identified
⢠Other developments (RDB trainings in 2015, RDB Med&BS development)
⢠Changes for the future â any recommendations from the LM?
⢠Future role of RCMs and DCF-Ââârelated meetings: best practices, coordination, cohesion and
common structure in line with emerging needs of DCF
TOR 4. EU MAP
⢠Discuss recommendations/ output of RCMs: List of proposed stocks, landing obligation, metiers
⢠Discuss design-Âââbased sampling in relation to DCF: does it fulfil DCF requirements?
TOR 5. Availability of data
⢠Overview of latest developments (DCF Database Feasibility Study and plans for a follow-Âââup
study to this)
TOR 6. AOB
⢠Agree on a list of recommendations relating to DCF (that MS will need to report on in their
AR2015) â COM will provide a compilation of proposed recommendations from LM & STECF
Plenaries in 2014 as input
⢠Prepare a list of recommended meetings for 2016 as guidance for MS
⢠Review and prioritize DCF-Ââârelated study proposals from RCMs, PGECON, EGs etc
⢠ICES update on workshop on concurrent sampling and plans to re-Âââevaluate survey
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