Вітаміноносні шипшини Національного дендрологічного парку “Софіївка”

The characteristics of the vitamin bеaring species and forms of Rosa L. introducted in the conditions of the dendrological park Sofiyivka are given in the article.Наведено характеристики вітаміноносних видових форм Rosa L., інтродукованих в умовах дендропарку “Софіївка”

The use of cytokines in the treatment of cornea inflammatory and dystrophic diseases (review)

Cytokines are unique biologically active substances that form the system of regulation of basic body functions that simultaneously with the nervous and endocrine systems maintain homeostasis under adverse conditions – the introduction of pathogens or violation of the integrity of tissues. Cytokines regulate the amplitude and duration of inflammatory and immune responses, so they are produced and secreted transiently and have a short half-life. These biologically active substances act in very low concentrations. At the same time, the main properties of cytokines are pleiotropy, lack of antigenic specificity, and the ability to form a cascade chain, that allows versatile correction of the spectrum of reparative processes. There are evidences of the possibility of using cytokines in the regeneration of damaged tissues in recent decades. In this connection, a new vector of search for pathogenetically directed ways to improve and restore lost functions using both individual representatives of cytokines and their natural complex is determined. The article reflects the basic information about the influence of cytokines on the development of various diseases of the cornea, as well as the methods of cytokine therapy in the treatment of these pathologies and prospects for their development

Features of the local cytokine profile of patients with bullous keratopathy by using personalized therapy with cellular technologies

Background. Today we have active development of ophthalmic surgery, but the role of cytokines in the pathogenesis of bullous keratopathy of the cornea remains insufficiently studied at present.The aim. To study the dynamics of the local cytokine profile in bullous keratopathy by using personalized treatment with suspension of autologous blood mononuclears.Material and methods. Two groups of patients with bullous keratopathy were formed: the main group (30 people, 30 eyes), who received an intrastromal injection of a suspension of autologous blood mononuclears and comparison (28 people, 28 eyes), who received a course of pharmacotherapy. The level of interleukins (IL) – IL-10, IL-4, IL-6, IL-1ß, transforming growth factor beta-2 (TGF-β2), tumor necrosis factor alpha (TNF-α) was determined in the lacrimal fluid of the patient’s eye by enzyme immunoassay.Results. All patients before treatment showed an increase of IL-1ß in the lacrimal fluid by 3.3 times, IL-6 – by 4.2 times, TNF-α – by 2.0 times (p < 0.05); an increase in the level of IL-4, IL-10 and TGF-β2 by 1.1 times (p > ˂ 0.05). In the main group, after treatment, there was a decrease in IL-1ß by 2.0 times, IL-6 – by 2.1 times and TNF-α – by 1.8 times, and an increase in IL-10 by 1.5 times, IL-4 – by 1.9 times, TGF-β2 – by 1.4 times (p < 0.05), persisting for 12 months. There was a short-term decrease of IL-1ß by 1.7 times, IL-6 and TNF-α – by 1.2 times and an increase of IL-10, IL-4, TGF-β2 by 1.2 times (p >< 0.05) in the comparison group which then reached the initial values. Conclusion. There is an imbalance in the system of pro- and anti-inflammatory cytokines in bullous keratopathy in the lacrimal fluid. Injection of autologous mononuclear cells in the stroma of the cornea reduces the severity of the imbalance of the local cytokine system compared to the course of pharmacotherapy. Key words: bullous keratopathy, cellular technologies, autologous mononuclear leukocytes, cytokines>˂ 0.05), persisting for 12 months. There was a short-term decrease of IL-1ß by 1.7 times, IL-6 and TNF-α – by 1.2 times and an increase of IL-10, IL-4, TGF-β2 by 1.2 times (p ˂ 0.05) in the comparison group which then reached the initial values.Conclusion. There is an imbalance in the system of pro- and anti-inflammatory cytokines in bullous keratopathy in the lacrimal fluid. Injection of autologous mononuclear cells in the stroma of the cornea reduces the severity of the imbalance of the local cytokine system compared to the course of pharmacotherapy

GA4GH: International policies and standards for data sharing across genomic research and healthcare.

The Global Alliance for Genomics and Health (GA4GH) aims to accelerate biomedical advances by enabling the responsible sharing of clinical and genomic data through both harmonized data aggregation and federated approaches. The decreasing cost of genomic sequencing (along with other genome-wide molecular assays) and increasing evidence of its clinical utility will soon drive the generation of sequence data from tens of millions of humans, with increasing levels of diversity. In this perspective, we present the GA4GH strategies for addressing the major challenges of this data revolution. We describe the GA4GH organization, which is fueled by the development efforts of eight Work Streams and informed by the needs of 24 Driver Projects and other key stakeholders. We present the GA4GH suite of secure, interoperable technical standards and policy frameworks and review the current status of standards, their relevance to key domains of research and clinical care, and future plans of GA4GH. Broad international participation in building, adopting, and deploying GA4GH standards and frameworks will catalyze an unprecedented effort in data sharing that will be critical to advancing genomic medicine and ensuring that all populations can access its benefits

Vitamin bearing dogroses in the National Dendrological Park Sofiyivka

The characteristics of the vitamin bеaring species and forms of Rosa L. introducted in the conditions of the dendrological park Sofiyivka are given in the article

Motif Elucidation in ChIP-seq Datasets with a Knockout Control

Chromatin immunoprecipitation-sequencing (ChIP-seq) is widely used to find transcription factor binding sites, but suffers from various sources of noise. Knocking out the target factor mitigates noise by acting as a negative control. Paired wild-type and knockout experiments can generate improved motifs but require optimal differential analysis. We introduce peaKO—a method to automatically optimize motif analyses with knockout controls, which we compare to two other methods. PeaKO often improves elucidation of the target factor and highlights the benefits of knockout controls. It is freely available at https://peako.hoffmanlab.org.M.Sc

Making parameters vibrating husked grain

The method and the results of calculation of parameters peeler considering rheology of grain and the speed of the drive disk