Clinical trials in children: Equity, quality and relevance

This thesis investigates the equity, quality and relevance of clinical trials in children to inform better evidence-based child healthcare and outcomes worldwide. A comprehensive review of the literature revealed that despite current initiatives to encourage more trials in children, there is still a paucity of safety and efficacy data of many medicines prescribed in this population. An analysis of trials registered in children showed that disease burden was moderately correlated to trials and this scarcity was particularly prevalent in low-and middle-income countries. We explored the contributory factors to this inequity by conducting a systematic review of stakeholders’ views of trials in children in low-and middle-income countries. In the study evaluating the completeness of protocols of trials in children submitted to ethics committees, we found that protocols are generally comprehensive, but many key domains in trial design and conduct are not reported. Key-informant trial stakeholders who were interviewed proposed strategies to improve trials such as addressing the unique needs of children, embedding trials as part of routine clinical care and streamlining regulatory approvals. Increasing international collaboration, establishing sustainable centralised trials infrastructure, and aligning research to child health priorities were proposed to encourage more high-quality trials that address global child healthcare needs

Hydraulic retention time affects bacterial community structure in an As-rich acid mine drainage (AMD) biotreatment process.

Arsenic removal consecutive to biological iron oxidation and precipitation is an effective process for treating As-rich acid mine drainage (AMD). We studied the effect of hydraulic retention time (HRT)-from 74 to 456 min-in a bench-scale bioreactor exploiting such process. The treatment efficiency was monitored during 19 days, and the final mineralogy and bacterial communities of the biogenic precipitates were characterized by X-ray absorption spectroscopy and high-throughput 16S rRNA gene sequencing. The percentage of Fe(II) oxidation (10-47%) and As removal (19-37%) increased with increasing HRT. Arsenic was trapped in the biogenic precipitates as As(III)-bearing schwertmannite and amorphous ferric arsenate, with a decrease of As/Fe ratio with increasing HRT. The bacterial community in the biogenic precipitate was dominated by Fe-oxidizing bacteria whatever the HRT. The proportion of Gallionella and Ferrovum genera shifted from respectively 65 and 12% at low HRT to 23 and 51% at high HRT, in relation with physicochemical changes in the treated water. aioA genes and Thiomonas genus were detected at all HRT although As(III) oxidation was not evidenced. To our knowledge, this is the first evidence of the role of HRT as a driver of bacterial community structure in bioreactors exploiting microbial Fe(II) oxidation for AMD treatment

Biological attenuation of arsenic and iron in a continuous flow bioreactor treating acid mine drainage (AMD)

International audienc

Multiscale modeling of the dynamical conductivity of self-assembled nanoparticle networks: Numerical simulations vs analytical models

Impedance spectroscopy experiments are able to reveal the fundamental charge transport properties of a wide variety of complex disordered and nano-structured materials provided that appropriate modeling tools are used. In this paper, we present a numerical simulation-based approach to model the dynamical conductivity of networks formed by self-assembled metal nanoparticles. Inter-particle nano-resistance and nano-capacitance are implemented at the nano-scale assuming inter-particle charge transfer and accumulation mechanisms that can be adapted depending on the nature of the nano-particles and the surrounding medium. The actual positions and spatial arrangements of the nanoparticles within the network are taken into consideration, allowing the attributes of percolating conducting routes to be extracted, classified, and compared in terms of path conductance and statistical distribution of path lengths. Our findings are contrasted to those obtained using analytic models, which are commonly used, but rely on strong assumptions about the electric properties of the conducting paths. We address these assumptions and show that in the case of weakly disordered systems, there is a general agreement between numerical simulations and analytic modeling-based approaches. In the case of disordered networks where the nano-particle size and position fluctuations are included, we show that the path length distribution is frequency-dependent and can differ significantly from the lognormal distribution usually assumed in the analytic models. The impedance of individual pathways may be extracted from the numerical simulations; we discovered that the conductance and susceptance of a specific path are frequency-dependent and inversely proportional to the path length only in ordered networks. Strong scattering of conductance values is caused by disorder effects. The developed numerical approach is generic and applies to most nano-devices where charge transport relies on percolation; it allows to bridge the gap between the nano-scale and micro-scale electric characteristics and, thus, permits a deeper understanding of the charge transport properties of nano-structured materials

Impact of citric acid on the impregnation of CoMoP/γ-Al 2 O 3 catalysts: time and spatially resolved MRI and Raman Imaging study

International audienceAn in-situ characterization methodology based on the Magnetic Resonance Imaging (MRI) and Raman Imaging is applied to investigate the effect of citric acid in the impregnation step of molybdenum catalysts promoted with cobalt in the presence of phosphorus and support on γ-alumina. MRI provides temporal and spatial information of the transport of species of the impregnation solutions within the porosity with a spatial resolution of 39×39µm. Raman Imaging gives information about the chemical nature of the species deposited on the support with a spatial resolution of 16.2×16.2µm. The effect of citric acid strongly depends on the ratio between the additive and molybdenum used in the impregnation solution. For a ratio of 0.2, at the end of impregnation, molybdenum ions are in an egg-yolk distribution either in polymeric or monomeric form. For a ratio of 0.7, cobalt ions can be in the form of aqua complexes in an egg-yolk distribution or in the form of H 2 PMo 11 CoO 40 5-heteropolyanion, which can improve the proximity between molybdenum and cobalt ions. This work gives new insights concerning the role of citric acid on the metal distribution profiles obtained at the end of impregnation, which can be used to control the final active phase distribution

Release of arsenite, arsenate and methyl-arsenic species from streambed sediment affected by acid mine drainage : a microcosm study

The (bio-)geochemical processes driving As mobilisation from streambed sediments affected by acid mine drainage (AMD) were investigated, and the structure of the bacterial community associated with the sediments was characterised. Microcosm experiments were set up to determine the effect of oxygen, temperature (4 and 20 degrees C) and microbial activity on As mobilisation from contrasting sediments collected during high- (November 2011) and low- (March 2012) flow conditions in the Amous River, that received AMD. Distinct bacterial communities thrived in the two sediments, dominated by Rhodobacter spp., Polaromonas spp. and Sphingomonads. These communities included only few bacteria known for their capacity to interact directly with As, whereas biogeochemical processes appeared to control As cycling. Major As mobilisation occurred in the As-III form at 20 degrees C in anoxic conditions, from both November and March sediments, as the result of successive biotic reductive dissolution of Mn- and Fe-oxyhydroxides. The later process may be driven by Mn- and Fe-reducing bacteria such as Geobacter spp. and possibly occurred in combination with microbially mediated As-V reduction. The involvement of other bacteria in these redox processes is not excluded. Biomethylation occurred only with the sediments collected at low-flow during oxic and anoxic conditions, although no bacteria characterised so far for its ability to methylate As was identified. Finally, sorption equilibrium of As-V onto the sediment appeared to be the main process controlling As-V concentration in oxic conditions. Comparison with field data shows that the later process, besides biomethylation, may be of relevance to the As fate in AMD-affected streams

Occurrence and removal of butyltin compounds in a waste stabilisation pond of a domestic waste water treatment plant of a rural French town

The aim of this study was to investigate the fate and behaviour of butyltin pollutants, including monobutyltin (MBT), dibutylin (DBT), and tributyltin (TBT), in waste stabilisation ponds (WSP). The study was conducted as part of a baseline survey and included five sampling campaigns comprising bottom sludge and the water column from each pond from a typical WSP in France. Butyltins were detected in all raw wastewater and effluents, reflecting their widespread use. Our results revealed high affinity between butyltins and particulate matter and high accumulation of butyltins in the sludge taken from anaerobic ponds. The dissolved butyltins in the influent ranged from 21.5 to 28.1 ng(Sn).L-1 and in the effluent, from 8.8 to 29.3 ng(Sn).L-1. The butyltin concentrations in the sludge ranged from 45.1 to 164 and 3.6-8.1 ng(Sn).g(-1) respectively in the first and last ponds. Our results showed an average treatment efficiency of 71% for MBT, 47% for DBT, 55% for TBT. Laboratory sorption experiments enabled the calculation of a distribution coefficient (Kd = 75,000 L.kg-1) between TBT and particulate matter from the WSPs. The Kd explained the accumulation and persistence of the TBT in the sludge after settling of particulate matter. The continuous supply of contaminated raw wastewater and the sorption-desorption processes in the ponds led to incomplete bio- and photolytic degradation and to the persistence of butyltins in dissolved and particulate matrices throughout the survey period. It is thus recommended to use shallow ponds and to pay particular attention when sludge is used for soil amendment