Inhibition of histamine-induced bronchospasm in guinea pigs treated with Cecropia glaziovi Sneth and correlation with the in vitro activity in tracheal muscles

A standardized aqueous extract (AE) and a purified fraction (BuF) of Cecropia glaziovi Sneth leaves were tested in unrestrained guinea pigs challenged with histamine. Changes of the respiratory pressure and rate were recorded in a whole body plethysmograph before and after treatment. the concentration of histamine necessary to produce bronchospasm was increased by five-fold following administration of AE (1.0 g/kg p.o.), and by two-fold after treatment with the semi-purified procyanidin/flavonoids enriched BuF (0.1 g/kg p.o.). Both effects were blocked by previous treatment with propranolol (10.0 mg/kg i.p.). in vitro incubation of BuF (0.1-1.0 mg/ml) decreased by 13-55% the maximal response of guinea pig tracheal muscle to histamine, without significant change of EC50. the results confirmed old reports on the useful pulmonary effects of Cecropia extracts. the bronchodilation observed in vivo seems to be related to beta-adrenergic activity observed in vitro only with high concentrations of the purified extract. (C) 2007 Elsevier GmbH. All rights reserved.Universidade Federal de São Paulo, Dept Pharmacol, Nat Prod Sect, Escola Paulista Med, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, Nat Prod Sect, Escola Paulista Med, BR-04044020 São Paulo, BrazilWeb of Scienc

Processo De Extração De Compostos Ativos De Material Vegetal

Processos de extração de compostos ativam de material vegetal. A presente invenção refere-se a um processo de extração de compostos ativas otimizado provido de um alto rendimento global, a partir de um material vegetal por meio da tecnologia do fluido supercritico, de modo a se obter um produto natural provido em sua composição química da substância espilantol ou ainda outras amidas e compostos ativas que conferem ao referido produto características bioativas responsáveis pela ação antiséptica, anti-inflamatória analgésica, inseticida dentre outras.BRPI0802052 (A2)A61K31/16A61P29/00A61P31/02B01D11/02BR2008PI02052A61K31/16A61P29/00A61P31/02B01D11/0

Technological development of Cecropia glaziovi extract pellets by extrusion-spheronization

Cecropia glaziovi Snethl., Urticaceae, is commonly used in South America and is one of the species included in the Brazilian Medicinal Plants Research Program. Pharmacological studies have led to reports of the potential of C. glaziovi as a hypotensive, antiasthmatic and anxiolytic agent. The strict requirements regarding the quality, safety and effectiveness of phytopharmaceutical products represent an enormous challenge in the search for products with a high level of uniformity, reproducibility and stability. The incorporation of dry extracts into multiparticulate dosage forms, such as pellets produced by extrusion/spheronization technology, is a suitable alternative to overcome the lack of technological properties of dry extracts, since they are associated with low flowability and high hygroscopicity. In this study, an optimized dry extract (ODE) of C. glaziovi was incorporated into pellets seeking to decrease the moisture sorption and increase the stability, safety and percentage of the extract in the final product. Pellets containing around 50% of ODE were considered the most technologically viable, offering a narrow particle size distribution, significant improvement in the flowability and compressibility properties, and decrease in the moisture compared with the ODE. In conclusion, pellets containing a high dose of the C. glaviovi extract were successfully prepared, achieving degrees of quality, physical stability and feasibility compatible with the desirable characteristics of a phytopharmaceutical