Doped-iron oxide nanocrystals synthesized by one-step aqueous route for multi-imaging purposes

New doped inorganic nanocrystals (NC) consisting on iron oxide and other metal integrated into the structure have been synthesized in one-step by adapting the oxidant precipitation synthesis route for magnetite. Different metals have been chosen to confer extra and unique properties to the resulting magnetic hetero-nanostructure: Co and Gd for enhancing transversal and longitudinal relaxivities for magnetic resonance imaging and Bi and Au for achieving X-ray absorption for computed tomography imaging. Apart of that, gold optical properties are interesting for photothermal therapy and iron oxides for magnetic hyperthermia. All metals have been incorporated into the magnetite structure in different ways during the synthesis: by forming a solid solution, by modifying the surface of the NCs, or by co-crystallization with the magnetite. The nanostructure formed in each case depends on the ionic radius of the secondary metal ion and the solubility of its hydroxide that control the co-precipitation in the initial steps of the reaction. Magnetic properties and imaging capabilities of the hetero-nanostructures have been analyzed as a function of the element distribution. Due to the synergistic combination of the different element properties, these magnetic hetero-nanostructures have great potential for biomedical applications

Whither Magnetic Hyperthermia? A Tentative Roadmap

The scientific community has made great efforts in advancing magnetic hyperthermia for the last two decades after going through a sizeable research lapse from its establishment. All the progress made in various topics ranging from nanoparticle synthesis to biocompatibilization and in vivo testing have been seeking to push the forefront towards some new clinical trials. As many, they did not go at the expected pace. Today, fruitful international cooperation and the wisdom gain after a careful analysis of the lessons learned from seminal clinical trials allow us to have a future with better guarantees for a more definitive takeoff of this genuine nanotherapy against cancer. Deliberately giving prominence to a number of critical aspects, this opinion review offers a blend of state-of-the-art hints and glimpses into the future of the therapy, considering the expected evolution of science and technology behind magnetic hyperthermia

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Development and Application of Nanoparticles in Biomedical Imaging

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SAXS analysis of single and multi core iron oxide magnetic nanoparticles

This article reports on the characterization of four superparamagnetic iron oxide nanoparticles stabilized with dimercaptosuccinic acid, which are suitable candidates for reference materials for magnetic properties. Particles p(1) and p(2) are single-core particles, while p(3) and p(4) are multi-core particles. Small-angle X-ray scattering analysis reveals a lognormal type of size distribution for the iron oxide cores of the particles. Their mean radii are 6.9 nm (p(1)), 10.6 nm (p(2)), 5.5 nm (p(3)) and 4.1 nm (p(4)), with narrow relative distribution widths of 0.08, 0.13, 0.08 and 0.12. The cores are arranged as a clustered network in the form of dense mass fractals with a fractal dimension of 2.9 in the multi-core particles p(3) and p(4), but the cores are well separated from each other by a protecting organic shell. The radii of gyration of the mass fractals are 48 and 44 nm, and each network contains 117 and 186 primary particles, respectively. The radius distributions of the primary particle were confirmed with transmission electron microscopy. All particles contain purely maghemite, as shown by X-ray absorption fine structure spectroscopy