Uso de insulina para aumento da fertilidade de vacas de corte pós-parto lactantes inseminadas

The objective of this work was to evaluate the effect of insulin alone or in association with equine chorionic gonadotropin (eCG) on the fertility of postpartum beef cows subjected to synchronization. A total of 340 cows was subjected to fixed time artificial insemination. In the trial 1, the cows were subjected to temporary weaning (TW), while in the trial 2 the same protocol was tested without TW. The addition of an insulin injection to a progesterone/eCG-based protocol without TW increased the pregnancy rate of beef cows with body condition score (BCS) equal to or lower than 2.5. Insulin had no effect on cows submitted to TW or with BCS equal to or higher than 3.0.O objetivo deste trabalho foi avaliar apenas o efeito da insulina ou dela em associação com gonadotrofina coriônica equina (eCG) na fertilidade de vacas em pós-parto submetidas à sincronização. Um total de 340 vacas foi submetido à inseminação artificial em tempo fixo. No experimento 1, as vacas foram submetidas ao desmame temporário (DT), enquanto no experimento 2, o mesmo protocolo foi testado sem DT. A adição de uma injeção de insulina a um protocolo baseado em progesterona/eCG sem DT aumentou a taxa de prenhez de vacas de corte com escore de condição corporal (ECC) igual ou menor que 2,5. A insulina não teve efeito em vacas submetidas a DT ou com ECC igual ou maior que 3,0

O uso de monensina sódica e probióticos para o controle de acidose ruminal subaguda em ovinos

O objetivo deste trabalho foi validar um protocolo para a indução de acidose ruminal subaguda (SARA) (Experimento 1) e testar a eficácia do probiótico Saccharomyces cerevisiae ou monensina na prevenção da queda do pH do fluido ruminal em ovinos (Experimento 2). No Experimento 1, seis ovelhas foram mantidas em jejum por dois dias e, em seguida, alimentadas basicamente com concentrado durante quatro dias. Nesse protocolo as ovelhas mantiveram o pH do fluido ruminal abaixo de 6,0 por 75 horas consecutivas. No Experimento 2, 18 ovelhas foram distribuídas em três grupos: controle (GC, n = 6), monensina (GM, n = 6) e o grupo probiótico (GP, n = 6). SARA foi induzida de acordo com o Experimento 1. PG apresentaram valores de pH mais baixos (5,7 ± 0,1) do que o GC (6,0 ± 0,1) (P = 0,05), enquanto GM (5,7 ± 0,1) foi semelhante durante a indução de SARA. A indução SARA reduziu a população de protozoários no rúmen (P < 0,05) e aumentou a concentração de cloreto no líquido ruminal (P < 0,01). Durante a SARA observou-se aumento das concentrações séricas de fósforo (P < 0,01), AST (P < 0,01) e GGT (P < 0,01), mas reduziu a de LDH (P < 0,01). Em conclusão, o protocolo utilizado para a indução de SARA foi capaz de manter o pH do rúmen entre 5,5-6,0 por períodos superiores a 48 horas. No entanto, a suplementação com monensina e probióticos não foi eficaz na prevenção das alterações nos parâmetros ruminais e séricos durante SARA.The aim of this work was to validate a protocol for induction of subacute ruminal acidosis (SARA) (Experiment 1) and test the efficiency of probiotic Saccharomyces cerevisiae or monensin to avoid pH ruminal drops in sheep (Experiment 2). In Experiment 1, six ewes were fasted for two days and then fed most with concentrate during four days. Ewes in this protocol had ruminal fluid pH below 6.0 and kept it for 75 consecutive hours. In Experiment 2, 18 sheep were distributed into three groups: Control (CG, n = 6), monensin (MG, n = 6) and probiotic group (PG, n = 6). SARA was induced according Experiment 1. PG had lower pH (5.7 ± 0.1) than CG (6.0 ± 0.1) (P = 0.05), while MG (5.7 ± 0.1) was similar to both during SARA induction. SARA induction reduced ruminal protozoa population (P < 0.05) and increased chloride concentrations in ruminal fluid (P < 0.01). In serum, SARA increased concentrations of phosphorus (P < 0.01), AST (P < 0.01) and GGT (P < 0.01), but reduced LDH (P < 0.01). In conclusion, the protocol used for SARA induction was able to maintain ruminal pH between 5.5-6.0 for more than 48 hours. However, monensin and probiotics supplementation was not effective in preventing changes in ruminal and serum parameters during SARA

Lesions associated with subclinical laminitis and digital radiographic exam in holstein cows in the transitional period

A laminite subclínica é responsável por grandes prejuízos na pecuária leiteira, sendo o seu diagnóstico de grande relevância para evitar reflexos negativos na produção. Portanto, o objetivo deste trabalho foi relacionar lesões associadas à laminite subclínica no período de transição de vacas da raça Holandês com a ocorrência ou não de sinais radiográficos vinculados a essa enfermidade. As vacas foram avaliadas durante o período de transição e divididas em dois grupos (com e sem sinais de laminite subclínica) de acordo com as lesões associadas a essa enfermidade subclínica. A dinâmica radiológica foi realizada em dois momentos durante o período de transição, com o propósito de determinar possíveis alterações de posicionamento capsular e degeneração óssea da terceira falange (F3). Não foram observados efeitos da presença de lesões associadas à laminite subclínica frente ao posicionamento da terceira falange à cápsula do casco (p>0,05). Diferenças radiográficas significativas (p0,05). Assim, neste estudo, vacas leiteiras no período de transição com lesões associadas à laminite subclínica não apresentaram sinais radiográficos de desalinhamento falangeano ao estojo córneo digital.Subclinical laminitis has been responsible for economic losses in dairy industry; thus, its diagnostic is very important to prevent negative responses to milk production industry. The aim of this study was to identify lesions associated with subclinical laminitis during the transition period with presence or absence of radiographic signs linked to this disease in Holstein cows. The cows were evaluated during the transition period and categorized into two groups (with and without laminitis) according to the lesions associated with this subclinical disease. The radiological dynamic was performed in two target points during the transition period (prepartum and postpartum), with the objective of determining capsular position possible alterations and third phalanx bone degeneration (F3). There was no effect of the presence of lesions associated with subclinical laminitis regarding the position of the third phalanx to the hoof wall (p>0.05). Significant radiographic angular differences (p0.05). Overall, this study did not show radiographic signs of phalangeal misalignment in the hoof of dairy cows with lesions associated with subclinical laminitis during the transition period

LESÕES ASSOCIADAS À LAMINITE SUBCLÍNICA E EXAME RADIOGRÁFICO DIGITAL EM VACAS DA RAÇA HOLANDÊS NO PERÍODO TRANSICIONAL

A laminite subclínica é responsável por grandes prejuízos na pecuária leiteira, sendo o seu diagnóstico de grande relevância para evitar reflexos negativos na produção. Portanto, o objetivo deste trabalho foi relacionar lesões associadas à laminite subclínica no período de transição de vacas da raça Holandês com a ocorrência ou não de sinais radiográficos vinculados a essa enfermidade. As vacas foram avaliadas durante o período de transição e divididas em dois grupos (com e sem sinais de laminite subclínica) de acordo com as lesões associadas a essa enfermidade subclínica. A dinâmica radiológica foi realizada em dois momentos durante o período de transição, com o propósito de determinar possíveis alterações de posicionamento capsular e degeneração óssea da terceira falange (F3). Não foram observados efeitos da presença de lesões associadas à laminite subclínica frente ao posicionamento da terceira falange      à cápsula do casco (p>0,05). Diferenças radiográficas significativas (p0,05). Assim, neste estudo, vacas leiteiras no período de transição com lesões associadas      à laminite subclínica não apresentaram sinais radiográficos de desalinhamento falangeano ao estojo córneo digital. Palavras-chave: casco; bovinos; exames complementares; pododermatite asséptica

Avaliação dos níveis de vitamina D3 e progesterona sob o comportamento alimentar de vacas leiteiras no pós-parto recente: Evaluation of vitamin D3 and progesterone levels under the feeding behavior of dairy cows in the recent postpartum period

O objetivo deste estudo foi investigar a influência da Progesterona (P4) e 25-hidroxivitamina D (VitD3) plasmática sobre o comportamento alimentar de vacas leiteiras durante a primeira semana pós-parto. Foram utilizadas 41 vacas da raça Holandês, multíparas, ECC de 3,0 a 3,5, de 2 a 4 lactações. Durante os dias 0 (dia do parto), 1, 2, 3 e 7 pós-parto foram mensurados o consumo, tempo de consumo, número de visitas ao cocho com e sem consumo, através dos alimentadores automáticos (Intergado®) e o tempo de ruminação, atividade e ócio a partir das coleiras inteligentes do tipo “C-TECH” (Cowmed Assistant®). Além disso, foram realizadas coletas sanguíneas nos mesmos dias para análise de VitD3, P4, glicose e ácidos graxos livres. A produção de colostro foi registrada até 7 dias pós-parto e a produção de leite foi registrada até 60 dias pós-parto através do software (DELPRO® Windows, DeLaval, MG, Brasil). No dia 0, os animais foram categorizados a partir dos níveis de VitD3, sendo que os animais que apresentaram níveis de VitD3 > 60ng/mL, foram alocados no grupo ALTAD3 (n=18), abaixo deste valor, foram direcionadas para o grupo MODERADAD3 (35 e 60ng/mL; n= 23). Os mesmos animais também foram categorizados em relação aos níveis de P4, sendo os que apresentaram concentrações de P4 > 0,70ng/mL foram alocados no grupo MAIORP4 (n=24) e abaixo deste valor (0,20 e 0,70ng/mL; n=17), no grupo MENORP4. Os grupos foram analisados separadamente entre ALTAD3 e MODERADAD3 e entre MENORP4 e MAIORP4. O grupo MENORP4 apresentou maior produção de colostro (30,03 ± 1,08; P = 0,03) e leite (44,95 ± 0,40; P = 0,02), maior número de visitas aos cochos com consumo (38,17 ± 1,55; P = 0,001) e tempo de ruminação (619,87 ± 18,49 ; P = 0,001) em comparação ao grupo MAIORP4. Além disso, o grupo ALTAD3 apresentou menor número de visitas aos cochos com (31,03 ± 1,72; P = 0,001) e sem (18,01 ± 1,73; P = 0,001) consumo do que os animais do grupo MODERADAD3. Houve correlação negativa entre os níveis de P4 e o tempo de ruminação nos grupos MENORP4 (r = -0,71; P = 0,01), ALTAD3 (r = -0,55; P = 0,03) e MODERADAD3 (r = -0,38; P = 0,01). A partir deste estudo, foi possível concluir, que os animais do grupo MENORP4 apresentaram maior produção de colostro e leite, número de visitas aos cochos com consumo e tempo de ruminação do que vacas do grupo MAIORP4. Já os animais do grupo ALTAD3 apresentaram menor número de visitas aos cochos com e sem consumo do que os animais do grupo MODERADAD3. Analisar os níveis P4 e VitD3, no dia do parto de vacas leiteiras, pode auxiliar no aperfeiçoamento do manejo das propriedades, pois, podem ter influência sob o comportamento alimentar e produção dos animais

Effect of GnRH or Estradiol Benzoate on Reproductive Traits during a Heatsynch Protocol in Dairy Cows

Background: Milk production of cows is closely correlated with its reproductive efficiency. One of the several factors influencing the dairy efficiency is the decline in fertility of the cows/heifers. Use of hormonal protocol are often used to improve the fertility of the recipients. Several programs are available to inseminate dairy cows, with variation in the use of different types of hormones, time of use and range of duration period, as well as the time of AI. The objective of this study was to evaluate the efficiency of follicular wave emergence induction of estradiol benzoate in comparison to GnRH in a Heatsynch protocol and its influence on reproductive parameters.Materials, Methods & Results: In this study were used primiparous and multiparous Holstein cows (n = 325). Animals were divided randomly into two different treatments according with the hormone used to induce follicular wave emergence (D0). The GnRH group (n = 167), was given 25 mg, while in the EB group (n = 158) was used 2 mg. At same day, were inserted, a controlled internal drug-releasing containing 1.9 g of progesterone CIDR®. On day 7, CIDR was removed and cows were given 25 mg luteinizing hormone followed by an injection of 1 mg Inducer Ovulation at day 8. Cows were then observed for signs of estrus for at least two hours (twice daily at 7 am and 6 pm) for three days following CIDR® removal. Cows detected in estrus were inseminated 12 h later the estrus detection. Pregnancy status was determined by ultrasonography at 30 ± 5 d and 60 ± 5 d after AI. A single technician was responsible for the estrus detection and inseminations. A single technician was responsible for the estrus detection and inseminations. Ultrassonographic examinations with ultrasound, transrectal linear probe of 5 MHz, were performed for the diagnosis of pregnancy at 30 and 60 days after artificial insemination. Binomially distributed data, such as, estrous response, conception rate, pregnancy rate and pregnancy losses, were analyzed by Chi-square test. Pregnancy rates at 30-35 days and 60-65 days as well as pregnancy losses were considered in the model for the effect of parity (primiparous vs. multiparous) and the group of estrus behavior during the Heatsynch protocol. Conception rate was similar between GnRH (36.1%, 57/158) and EB (37.7%, 63/167) treatments. Cows showing estrus signs after CIDR removed were 71.9% in GnRH and 62% in EB group (P = 0.05). Embryonic losses were different (P = 0.03) according to groups of cows showing estrus signals or not.Discussion: Our results demonstrate that conception rates are not affected by using either EB or GnRH at the beginning of the Heatsynch protocol. Therefore, the EB can be an alternative choice to GnRH at Heatsynch beginning. Similar results were found by autor in dairy cows, once it was also observed that there is no difference in conception rate in two TAI protocols using either GnRH or EB. In Heatsynch protocols the increase in estrus detection are a key factor to increase fertility in lactating dairy cows. It can be explained once animals in regular estrous cycle have a palpable corpus luteum and circulating progesterone concentration higher than 1 ng/mL which leads than to enhance pregnancy ratios. The use of BE at the time of CIDR insertion in TAI protocols for dairy cattle proved to be an alternative to GnRH considering conception rates. Therefore, the use of BE at the time of CIDR insertion in a Heatsynch protocol in dairy cattle proved to be an alternative to GnRH which can be an important tool to increase the TAI utilization by farmers. Furthermore, in Heatsynch protocol cows showing estrus have pregnancy rates improved and lower pregnancy losses

Effect of porcine somatotropin on metabolism and testicular characteristics of prepubertal pigs

Foi investigado o efeito da pST sobre características testiculares e metabolismo de suínos pré-púberes. O Experimento 1 determinou o intervalo entre aplicações de pST, baseado nas concentrações de IGF-I. O Experimento 2 avaliou o efeito da pST sobre o metabolismo, características testiculares e expressão gênica de GHR, IGF-I e PCNA. No Experimento 1 foram usados 12 leitões com 30 dias de idade. O grupo pST (n=6) foi submetido a uma injeção IM de pST e o grupo Controle (n=6) a uma injeção de placebo. Coletas de sangue foram realizadas até o dia sete após aplicação de pST para determinação dos níveis de IGF-I e parâmetros metabólicos. No Experimento 2 foram usados 12 leitões com 22 dias de idade. O grupo pST foi submetido à aplicações de pST a cada 3 dias, e o grupo Controle à doses de placebo, durante 30 dias. Coletas de sangue foram realizadas a cada três dias. Amostras de fígado e testículo foram coletadas para determinar a expressão gênica e características testiculares. No Experimento 1 a concentração de IGF-I foi maior no grupo pST (P=0,02). No Experimento 2 o grupo pST teve maior peso corporal e testicular (P=0,06) e aumento na expressão de PCNA no testículo (P<0,05). Contudo, foi observada uma redução no número de túbulos seminíferos, células de Sertoli e GHR (P<0,05). Assim, a administração de pST aumentou o desenvolvimento testicular e corporal de suínos pré-púberes, porém reduziu a densidade de túbulos seminíferos e células de Sertoli. The effect of pST on the testicular characteristics and metabolic parameters of prepubertal pigs was evaluated. Experiment 1 aimed to determine the interval between applications of pST based on the concentrations of circulating IGF-I. Experiment 2 aimed to evaluate the effect of pST on metabolic parameters, testicular characteristics, and expression of GHR, IGF-I and PCNA. In Experiment 1 twelve piglets with 30 days of age were used. The pST Group (n=6) was submitted to one i.m. injection of pST and the Control Group (n=6) to one placebo injection. Blood collections were performed until d 7 post pST application to determine IGF-I concentration and metabolic profile. In Experiment 2 twelve piglets with 22 days of age were used. The pST Group was submitted to pST injections every three days, and the Control Group received placebo doses during 30 days. Blood collections were performed every 3 days. Samples of liver and testicular tissue were collected to determine gene expression and testicular characteristics. In Experiment 1 IGF-I concentration was higher for the pST Group (P=0.02). In Experiment 2 the pST Group had higher body and testicular weight (P=0.06) and increased gene expression of PCNA in testes (P<0.05). However, a reduction in the number of seminiferous tubules, and Sertoli cells, and in GHR expression (P<0.05) was observed. Thus, pST administration increased body and testis development in prepubertal pigs, however it reduced the density of seminiferous tubules and Sertoli cells

Effect of porcine somatotropin on metabolism and testicular characteristics of prepubertal pigs

The effect of pST on the testicular characteristics and metabolic parameters of prepubertal pigs was evaluated. Experiment 1 aimed to determine the interval between applications of pST based on the concentrations of circulating IGF-I. Experiment 2 aimed to evaluate the effect of pST on metabolic parameters, testicular characteristics, and expression of GHR, IGF-I and PCNA. In Experiment 1 twelve piglets with 30 days of age were used. The pST Group (n=6) was submitted to one i.m. injection of pST and the Control Group (n=6) to one placebo injection. Blood collections were performed until d 7 post pST application to determine IGF-I concentration and metabolic profile. In Experiment 2 twelve piglets with 22 days of age were used. The pST Group was submitted to pST injections every three days, and the Control Group received placebo doses during 30 days. Blood collections were performed every 3 days. Samples of liver and testicular tissue were collected to determine gene expression and testicular characteristics. In Experiment 1 IGF-I concentration was higher for the pST Group (P=0.02). In Experiment 2 the pST Group had higher body and testicular weight (P=0.06) and increased gene expression of PCNA in testes (P<0.05). However, a reduction in the number of seminiferous tubules, and Sertoli cells, and in GHR expression (P<0.05) was observed. Thus, pST administration increased body and testis development in prepubertal pigs, however it reduced the density of seminiferous tubules and Sertoli cells

Use of the Rumination Profile Through Collar Sensors for Mastitis Diagnosis in Dairy Cows

Background: Mastitis is an inflammatory disease of the mammary gland, mostly associated with bacterial infections. It is responsible for great economic losses due to decreased milk yield, discarded milk, milk composition alterations and treatment costs, besides it impairs the animal health and welfare. The rumination time is an important behavioral marker and its assessment can be used as an early diagnosis tool, which can improve cure rate. Therefore, the aim of the present study was to evaluate the sensitivity of behavior monitoring system collars in the diagnosis of mastitis and the average rumination time (RT) of Holstein cows during the healthy period and affected by the disease.Materials, Methods & Results: The study was conducted on a commercial property located in the municipality of Rio Grande, Rio Grande do Sul, Brazil. The RT data from 39 multiparous Holstein cows with an average milk yield of 38.4 L/day was collected. RT monitoring was performed using C-Tech1 collars combined with CowMed® software, which assess behavior data from the animals and emits warning signals when it finds abnormalities in any parameter. In order to verify whether the animals were determined correlated with diseases, the sensitivity of the data was evaluated, when the system had given the alert to animals considered ill, they underwent to a further clinical evaluation performed by a veterinarian to confirm the diagnosis. From the diagnosis, the cows were divided into subclinical mastitis (SM) and clinical mastitis (CM) groups. SM was detected by the Californian Mastitis Test (CMT) and cows that were graded 1 (++), 2 (++) or 3 (+++) without the presence of any other clinical sign were assigned to the SM group. CM was assessed by observation of abnormalities in milk such as changes in color and consistency, as well as the presence of lumps, clots or blood; and clinical examination of the udder was performed for detection of hot, hard, swollen or painful quarters. Thereafter, variations in the mean RT between the healthy (15 days) and sick periods (days when there was an alert) were evaluated. For the identification of the etiological agents involved in the cases of CM, microbiological cultures were performed on Accumast® plates with milk samples, which were incubated at a temperature of 37°C and the diagnosis of the pathogens were performed after16 h of incubation. During the study, 57 cases of mastitis were observed, 42 were SM and 15 were CM. The sensitivity of the system, which is the ability to detect positive cases of the disease, was 73.8% for SM and 73.3% for MC. The RT of the animals were compared individually during the healthy period with the sick period and it was observed that SM reduced the RT by 5.33% whereas MC reduced the RT by 14.9%.Discussion: The maximum RT values were lower during the disease period in relation to the period in which the animals were healthy, for both SM and CM, which is due to the fact that the disease is responsible for causing inappetence in animals, among other clinical signs, therefore, reducing feed consumption. The lowest variation in RT, between the healthy and sick period, was observed in cases of SM, which was already expected, since the clinical form tends to cause more discomfort to the animals. As for the main etiological agents involved in the clinical condition, Streptococcus agalactiae and S. uberis were detected. In view of the above, the evaluation of the mean RT of multiparous dairy cows was efficient in the predictive diagnosis of SM and CM up to 2 days before the onset of the disease. In addition, the variability of this result demonstrated that animals with subclinical cases presented less fluctuation in RT

Utilização de sulfonamidas para o tratamento de diarreia neonatal bovina: parâmetros clínicos e zootécnicos

Este estudo foi desenvolvido com o objetivo de avaliar parâmetros clínicos, hematológicos e zootécnicos de bezerras acometidas por diarreia provocada pelo agente bacteriano Escherichia coli, tratadas com três diferentes protocolos contendo sulfonamidas. Quatorze bezerras da raça Holandês foram monitoradas do nascimento até os 60 dias de vida e divididas em 3 grupos: Grupo 1 (n=5), animais tratados com a dose de 80g de Kaopek®, Ibasa, Brasil, por via oral (VO), dissolvido em 160ml de água a cada 24 horas; Grupo 2 (n=4) receberam sulfadiazina e trimetropim (Ibatrim®, Ibasa, Brasil), na dose de 16mg/Kg de peso vivo, por dia, por via intramuscular (IM); Grupo 3 (n=5) 80g de Kaopek®, Ibasa, Brasil, por VO dissolvido em 160ml de água, a cada 24 horas e com 16mg/Kg de peso vivo, por dia, de sulfadiazina e trimetoprim (Ibatrim®, Ibasa, Brasil), por via IM. Durante o estudo, os animais foram avaliados clinicamente e a partir do diagnóstico de diarreia foram coletadas amostras de fezes para a identificação do agente bacteriano, antibiograma, reação em cadeia da polimerase (PCR) e exames coproparasitológicos. Amostras de sangue foram coletadas para avaliação do perfil hematológico e o perfil zootécnico foi acompanhado semanalmente. No exame clínico, todas as bezerras apresentaram redução na temperatura corporal (&lt;39,2°C) e melhora na hidratação após o período de tratamento (p=0,31), porém o grupo 2 obteve maior concentração de linfócitos e PPT em relação aos demais, assim como melhor desempenho zootécnico, além disso em 100% das amostras de fezes foi detectado E. coli. Sendo assim, os protocolos terapêuticos com sulfonamidas utilizados para o tratamento da diarreia neonatal bovina foram eficazes na melhora clínica dos animais. Porém, o protocolo com terapia sistêmica com sulfadiazina e trimetoprim (Ibatrim®, Ibasa, Brasil) por via intramuscular proporcionou um melhor desempenho zootécnico, com melhor ganho de peso e desenvolvimento corporal dos animais.This study was developed to evaluate the clinical, hematological, and performance parameters of calves affected by diarrhea caused by the bacterial enteric pathogen Escherichia coli, treated with three different protocols containing sulfonamides. Fourteen Holstein calves were monitored from birth to 60 days of age, and divided into 3 groups. Group 1 (n=5) animals were treated with 80 g of Kaopek®, Ibasa, Brazil, administered orally (BM), dissolved in 160 ml of water every 24 h; Group 2 (n=4) received IM sulfadiazine and trimethoprim (Ibatrim®, Ibasa, Brazil), and Group 3 (n=5) animals were treated with 80 g of Kaopek®, Ibasa, Brazil, which is made up of 16 g of phthalylsulfathiazole, associated with 2.28 g neomycin sulfate, 1.6 g of pectin, and 80 g of kaolin, dissolved in 160 ml of water and administered every 24 h by mouth, in addition to sulfadiazine and trimethoprim (Ibatrim®, Ibasa, Brazil), at a daily dose of 16 mg/kg live weight, through IM injection. During the study, the animals were clinically evaluated, and once they were diagnosed with diarrhea, feces samples were collected to identify the bacterial enteric pathogen, antibiogram, polymerase chain reaction (PCR), and coproparasitological exams. Blood samples were collected to evaluate the hematological profile, and the performance profile was monitored weekly. In the clinical examination, all calves presented a reduction in body temperature (&lt;39.2°C) and some improvement in hydration after treatment (p=0,31). However, group 2 had a better concentration of lymphocytes and TP concerning the other animals, as well as better performance. Besides, E.coli was detected in 100% of feces samples. Thus, the therapeutic protocols with sulfonamides used to treat bovine neonatal diarrhea were effective in the clinical improvement of the animals. Nonetheless, the protocol with systemic therapy using sulfadiazine and trimethoprim (Ibatrim®, Ibasa, Brazil) intramuscularly, provided better performance, with better weight gain, and body development of the animals.