Technological research methodology to manage organizational change

Change is a process that is part of the nature of people; however, within organizations, it should be seen as an invention that will generate benefits in the markets. The main objective of this work is to design a technological methodology to manage change from seven administrative models. For this, a bibliographic review was carried out; the method applied was analysis-synthesis; the example technique was used to comment; the support instruments were a standard data collection form and a comparative table to analyze this data vertically and horizontally. The methodology was validated in the research units of a local university. The main results were 1) The change of management within organizations is an invention; 2) administrative models of change are used to manage it; 3) but, to be successful in managing change, the methodology of technological research is required in addition to the administrative process

Identification of seed-related QTL in Brassica rapa

To reveal the genetic variation, and loci involved, for a range of seed-related traits, a new F2 mapping population was developed by crossing Brassica rapa ssp. parachinensis L58 (CaiXin) with B. rapa ssp. trilocularis R-o-18 (spring oil seed), both rapid flowering and self-compatible. A linkage map was constructed using 97 AFLPs and 21 SSRs, covering a map distance of 757 cM with an average resolution of 6.4 cM, and 13 quantitative trait loci (QTL) were detected for nine traits. A strong seed colour QTL (LOD 26) co-localized with QTL for seed size (LOD 7), seed weight (LOD 4.6), seed oil content (LOD 6.6), number of siliques (LOD 3) and number of seeds per silique (LOD 3). There was only a significant positive correlation between seed colour and seed oil content in the yellow coloured classes. Seed coat colour and seed size were controlled by the maternal plant genotype. Plants with more siliques tended to have more, but smaller, seeds and higher seed oil content. Seed colour and seed oil content appeared to be controlled by two closely linked loci in repulsion phase. Thus, it may not always be advantageous to select for yellow-seededness when breeding for high seed oil content in Brassicas

Efectos de una intervención educativa sobre la integridad científica en estudiantes universitarios

Introduction:  Universities have an essential role in shaping scientific integrity in their students. Despite its importance, few interventions have been studied to improve it. Objective: To evaluate the effects of an educational intervention on scientific integrity in university students in the southern region of Peru.  Methodology: An educational intervention was applied in three phases (pretest, intervention, and post-test). Robust comparison tests were applied for dependent samples, as well as AKP effect size measures. Mixed ANOVA tests were applied. Pairwise comparisons were used using Bonferroni\u27s adjusted p. method. Results: A total of 402 students (63.93% female) participated. The comparison of dependent samples between the post-test and pretest measures found statistically significant differences in the knowledge dimension (tyuen = 3.93; gl = 240; p < .001; AKP = 0.22 [95% CI: 0.12 – 0.33]) and in the practical dimension (tyuen = 4.88; gl = 240; p < .001; AKP = 0.30 [95% CI: 0.20 – 0.42]) and region (2,392) = 4.16, p < .001, ?2 = 0.008. Discussion: The intervention was effective according to the post-test evaluation, with some regional differences.Introducción: Las universidades tienen un rol importante en la formación de la integridad científica en sus estudiantes. A pesar de su importancia, se han estudiado pocas intervenciones que la mejoren. Objetivo: Evaluar los efectos de una intervención educativa sobre la integridad científica en estudiantes universitarios de la región sur del Perú.  Metodología: Se aplicó una intervención educativa en tres fases (pretest, intervención y postest). Se aplicaron pruebas de comparación robustas para muestras dependientes, así como también medidas de tamaño del efecto AKP. Se aplicaron pruebas ANOVA mixtos. Se utilizaron las comparaciones por pares siguiendo el método de la p. ajustada de Bonferroni. Resultados: Participaron 402 estudiantes (63.93% de sexo femenino). La comparación de muestras dependientes entre las medidas postest y pretest encontró diferencias estadísticamente significativas en la dimensión conocimientos (tyuen = 3.93; gl = 240; p < .001; AKP = 0.22 [IC 95%: 0.12 – 0.33]) y en la dimensión prácticas (tyuen = 4.88; gl = 240; p < .001; AKP = 0.30 [IC 95%: 0.20 – 0.42]) y la región (2,392) = 4.16, p < .001, ?2 = 0.008. Discusión: La intervención fue efectiva según la evaluación en el postest con algunas diferencias según regiones

Energy Landscape and Global Optimization for a Frustrated Model Protein

The three-color (BLN) 69-residue model protein was designed to exhibit frustrated folding. We investigate the energy landscape of this protein using disconnectivity graphs and compare it to a Go model, which is designed to reduce the frustration by removing all non-native attractive interactions. Finding the global minimum on a frustrated energy landscape is a good test of global optimization techniques, and we present calculations evaluating the performance of basin-hopping and genetic algorithms for this system.Comparisons are made with the widely studied 46-residue BLN protein.We show that the energy landscape of the 69-residue BLN protein contains several deep funnels, each of which corresponds to a different β-barrel structure

DISEÑO Y DESARROLLO DE UN PROTOTIPO A PARTIR DE ENVASES RECICLADOS

This work deals with Tetra-Pak cartons waste, and with the development of a board-shaped commercial prototype. A comparative analysis of technical specifications related to such 30x30x1 cm. size wooden-board is shown.El estudio trata sobre los desechos de envases llamados Tetra Pak y el desarrollo de un prototipo comercial en forma de tablas. Se muestra un análisis comparativo de especificaciones técnicas en relación con las tablas de madera, de dimensiones 30x30x1cm

Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution

Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, and plasma membrane integrity of alpaca spermatozoa after centrifugation in one of two different solutions at one of three different combinations of speed and time. Methods: A total of 24 ejaculates was recovered from seven reproductively sound Huacaya males using a modified artificial vagina (AV) after training the animals for semen collection. A 2 × 3 factorial treatment arrangement was utilized for this study. Ejaculates were divided into fractions for centrifugation in one of two solutions (Tris extender or PureSperm®80 density gradient solution) at one of three combinations of speed and time (492 × g for 15 min, 1968 × g for 10 min, or 4448 × g for 7 min). The experiment was replicated eight times. Results: Analysis revealed that centrifugation at 4448 × g for 7 min in PureSperm®80 provided a high recovery rate of spermatozoa with the highest sperm motility and functional integrity of plasma membrane post-centrifugation. Conclusion: Results suggest that adoption of this procedure (centrifugation at 4448 × g for 7 min in PureSperm®80) in the initial processing of alpaca ejaculates may enhance subsequent ability to use semen for AI and other assisted reproductive biotechnologies in this speciesFil: Gómez Quispe, O. E.. Universidad Nacional Agraria La Molina; Perú. Universidad Nacional Micaela Bastidas de Apurímac; PerúFil: Gutiérrez Reynoso, G. A.. Universidad Nacional Agraria La Molina; PerúFil: Gallegos Cardenas, A.. Universidad Nacional Agraria La Molina; PerúFil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asparrin, M.. Fundo Mallkini de Michael & Cia S.A.; PerúFil: Asparrin Del Carpio, M.. Fundo Mallkini de Michael & Cia S.A.; PerúFil: Jara, C. W.. Fundo Mallkini de Michael & Cia S.A.; PerúFil: Ponce, D.. Vivanco International SAC; PerúFil: Miguel, M.. Vivanco International SAC; PerúFil: Youngs, C. R.. Iowa State University; Estados UnidosFil: Vivanco, H. W.. Vivanco International SAC; Per

Regulatory Network of Secondary Metabolism in Brassica rapa: Insight into the Glucosinolate Pathway

Brassica rapa studies towards metabolic variation have largely been focused on the profiling of the diversity of metabolic compounds in specific crop types or regional varieties, but none aimed to identify genes with regulatory function in metabolite composition. Here we followed a genetical genomics approach to identify regulatory genes for six biosynthetic pathways of health-related phytochemicals, i.e carotenoids, tocopherols, folates, glucosinolates, flavonoids and phenylpropanoids. Leaves from six weeks-old plants of a Brassica rapa doubled haploid population, consisting of 92 genotypes, were profiled for their secondary metabolite composition, using both targeted and LC-MS-based untargeted metabolomics approaches. Furthermore, the same population was profiled for transcript variation using a microarray containing EST sequences mainly derived from three Brassica species: B. napus, B. rapa and B. oleracea. The biochemical pathway analysis was based on the network analyses of both metabolite QTLs (mQTLs) and transcript QTLs (eQTLs). Colocalization of mQTLs and eQTLs lead to the identification of candidate regulatory genes involved in the biosynthesis of carotenoids, tocopherols and glucosinolates. We subsequently focused on the well-characterized glucosinolate pathway and revealed two hotspots of co-localization of eQTLs with mQTLs in linkage groups A03 and A09. Our results indicate that such a large-scale genetical genomics approach combining transcriptomics and metabolomics data can provide new insights into the genetic regulation of metabolite composition of Brassica vegetables

Anemia infantil en poblaciones residentes en diferentes altitudes geográficas de Arequipa, Perú: factores maternos asociados

Objective: Determine the relationship between socio-demographic characteristics, knowledge, and good practices in anemic children and anemic child healthcare in Arequipa, Peru. Study design:  This is a cross-sectional survey. Participants were asked about their knowledge of childhood anemia, practices related to caring for anemic children, and adherence to treatment. Methods: This is a descriptive, transversal, relational field study. Three hundred mothers with anemic children were interviewed, from six districts of Arequipa, distributed at different geographic altitudes: medium-altitude, great-altitude, and sea level. Studied variables were related to socio-demographic factors, level of knowledge, anemic children care-related practices, and treatment adherence.  Results: 77.3% of children had mild anemia, 21.6% moderate, and 1% severe anemia. The number of children and disease severity were significantly related (p<0.05) to knowledge about anemia. Knowledge about anemia, living area, and geographic altitude were significantly correlated (p<0.05) with good practices in child anemia. Conclusion: the prevalence of anemia in the region of Arequipa is incremented by socio-economic factors, mother’s education, number of children, altitude, and geographic zone. The implementation of mother-centered educational programs by the government is necessary. In this sense, the results suggest further studies in social representations to understand, analyze and deepen the knowledge of the subject.Objetivo: Determinar la relación entre las características sociodemográficas, conocimientos y buenas prácticas en anemia infantil y la atención al niño anémico en Arequipa, Perú. Diseño del estudio: Se trata de una encuesta transversal. Se preguntó a los participantes sobre su conocimiento sobre la anemia infantil, las prácticas relacionadas con el cuidado de los niños anémicos y la adherencia al tratamiento. Métodos: Se trata de un estudio de campo, descriptivo, transversal, relacional. Se entrevistaron 300 madres con niños anémicos, de seis distritos de Arequipa, distribuidos en diferentes altitudes geográficas: mediana, gran y nivel del mar. Las variables estudiadas se relacionaron con factores sociodemográficos, nivel de conocimiento, prácticas relacionadas con el cuidado del niño anémico y adherencia al tratamiento. Resultados: El 77,3% de los niños presentaron anemia leve, el 21,6% moderada y el 1% anemia severa. El número de hijos y la gravedad de la enfermedad se relacionaron significativamente (p<0,05) con el conocimiento sobre la anemia. Los conocimientos sobre anemia, lugar de residencia y altitud geográfica se correlacionaron significativamente (p<0,05) con las buenas prácticas en anemia infantil. Conclusión: la prevalencia de anemia en la región de Arequipa se ve incrementada por factores socioeconómicos, escolaridad de la madre, número de hijos, altitud y zona geográfica. Es necesaria la implementación de programas educativos centrados en la madre por parte del gobierno. En ese sentido, los resultados sugieren profundizar los estudios en representaciones sociales para comprender, analizar y profundizar  el conocimiento del tema