Changes in understory vegetation of a ponderosa pine forest in northern Arizona 30 years after a Wildfire

Wildland fires can cause shifts in understory species composition and production. Many studies have examined short-term changes in understory vegetation following a wildfire; however, very few long term studies are available. The objective of this study was to examine changes in understory (herb and shrub) species composition and production since the 1972 Rattle Burn wildfire on the Coconino National Forest near Flagstaff, Arizona. Understory species composition and production were originally sampled in 1972, 1974, and 1980 and were re-sampled during July and August of 2002 and 2003 on 30 plots in each of four sites: high severity burn, low severity burn, unburned site prescribed burned in 1977, and an unburned site. Repeated measures analysis was used to test for the effects of fire and time on species production. The effects of fire and time on species composition as well as species production were tested using Multi-Response Permutation Procedures (MRPP). A lingering effect of the Rattle Burn wildfire on the understory plant production and composition was revealed. Burned sites may have greater understory production as compared to unburned sites up to 30 years after a wildfire. However, species composition on burned sites is altered. A significant relationship between tree density and understory species composition and production was found for 1972, but no relationship was found for overstory parameters and understory species production and composition for 2003

Recommendations for reducing energy consumption and improving air quality through energy efficiency in Indian Country: Working paper series--04-04

The strongest recommendation stemming from the Air Pollution Prevention forum of the Western Regional Air Partnership (WRAP) was for tribes to develop and implement energy plans. This paper provides a discussion of the benefits of any such plan and itemized a variety of components of any said plan. A brief discussion of the WRAP is presented is the framework of improving air quality. Based on the background of Jacobs (2000) and Smith (2000), the primary and secondary benefits of energy efficiency plans are then discussed. Then a series of specific recommendations are itemized. These include tribally sponsored programs, collaborative opportunities, and tribal leadership programs

Economic analysis of energy efficiency measures: Tribal case studies with The Confederated Salish and Kootenai Tribes of The Flathead Reservation, The Pascua Yaqui Tribe, and The Yurok Tribe: Working paper series--04-02

The best way to reduce air pollution from the production of electricity is not to produce it in the first place. This paper presents three cases studies explaining how energy efficiency programs can be beneficial to tribes from a purely cost-saving basis, The Yurok, Flathead and Pascua Yaqui Reservations graciously provided an opportunity to investigate energy efficiency for their tribes. In each case the consumption of electricity was reduced such that a positive net present value was present following an initial capital investment. In the case of the irrigation systems on the Flathead Reservation, the savings were substantial even though no explicit savings of water was accounted for. It is estimated that tribes can reduce electricity costs by 10-50% by following energy efficiency programs. Many programs can be very simple involving small upfront capital outlays, such as changing light bulbs or installing motion sensors. The Yurok case showed a return of 7.5% after simply changing bulbs and the Pasqua Yaqui case showed a possible return of 10-80%. Other tribes could make similar investments and realize similar benefits in addition to improved air quality. The strongest recommendation from the Air Pollution Prevention Forum to tribes was to develop an energy plan. These cases show that some fairly simple analysis can make a plan very beneficial

Identification and implementation of potential energy efficiency programs in Indian Country: Working paper series--04-03

The strongest recommendation stemming from the Air Pollution Prevention forum of the Western Regional Air Partnership (WRAP) was for tribes to develop and implement energy plans. This paper provides a discussion of the benefits of any such plan and itemized a variety of components of any said plan. A brief discussion of the WRAP is presented is the framework of improving air quality. Based on the background of Jacobs (2000) and Smith (2000), the primary and secondary benefits of energy efficiency plans are then discussed. Then a series of specific recommendations are itemized. These include tribally sponsored programs, collaborative opportunities, and tribal leadership programs

Effect of Phosphorus Amendments on Present Day Plankton Communities in Pelagic Lake Erie

To address questions regarding the potential impact of elevated total phosphorus (TP) inputs (due to relaxed regulations of TP loading), a series of TP enrichment experiments were conducted at pelagic stations in the 3 hydrologically distinct basins of Lake Erie. Results of nutrient assimilation measurements and assays for nutrient bioavailability suggest that the chemical speciation, and not concentration, of nitrogenous compounds may influence phytoplankton community structure; this in turn may lead to the selective proliferation of cyanobacteria in the eastern basin of the lake. Assays with cyanobacterial bioluminescent reporter systems for P and N availability as well as N-tot:P-tot assimilation ratios from on-deck incubation experiments support this work. Considered in the context of a microbial food web relative to a grazing food web, the results imply that alterations in current TP loading controls may lead to alterations in the phytoplankton community structure in the different basins of the Lake Erie system

A feasibility study into the production of a mussel matrix reference material for the cyanobacterial toxins microcystins and nodularins.

Microcystins and nodularins, produced naturally by certain species of cyanobacteria, have been found to accumulate in aquatic foodstuffs such as fish and shellfish, resulting in a risk to the health of the seafood consumer. Monitoring of toxins in such organisms for risk management purposes requires the availability of certified matrix reference materials to aid method development, validation and routine quality assurance. This study consequently targeted the preparation of a mussel tissue reference material incurred with a range of microcystin analogues and nodularins. Nine targeted analogues were incorporated into the material as confirmed through liquid chromatography with tandem mass spectrometry (LC-MS/MS), with an additional 15 analogues detected using LC coupled to non-targeted high resolution mass spectrometry (LC-HRMS). Toxins in the reference material and additional source tissues were quantified using LC-MS/MS, two different enzyme-linked immunosorbent assay (ELISA) methods and with an oxidative-cleavage method quantifying 3-methoxy-2-methyl-4-phenylbutyric acid (MMPB). Correlations between the concentrations quantified using the different methods were variable, likely relating to differences in assay cross-reactivities and differences in the abilities of each method to detect bound toxins. A consensus concentration of total soluble toxins determined from the four independent test methods was 2425 ± 575 µg/kg wet weight. A mean 43 ± 9% of bound toxins were present in addition to the freely extractable soluble form (57 ± 9%). The reference material produced was homogenous and stable when stored in the freezer for six months without any post-production stabilization applied. Consequently, a cyanotoxin shellfish reference material has been produced which demonstrates the feasibility of developing certified seafood matrix reference materials for a large range of cyanotoxins and could provide a valuable future resource for cyanotoxin risk monitoring, management and mitigation

The state of the focus and image quality of the Spitzer Space Telescope as measured in orbit

We describe the process by which the NASA Spitzer Space Telescope (SST) Cryogenic Telescope Assembly (CTA) was brought into focus after arrival of the spacecraft in orbit. The ground rules of the mission did not allow us to make a conventional focus sweep. A strategy was developed to determine the focus position through a program of passive imaging during the observatory cool-down time period. A number of analytical diagnostic tools were developed to facilitate evaluation of the state of the CTA focus. Initially, these tools were used to establish the in-orbit focus position. These tools were then used to evaluate the effects of an initial small exploratory move that verified the health and calibration of the secondary mirror focus mechanism. A second large move of the secondary mirror was then commanded to bring the telescope into focus. We present images that show the CTA Point Spread Function (PSF) at different channel wavelengths and demonstrate that the telescope achieved diffraction limited performance at a wavelength of 5.5 μm, somewhat better than the level-one requirement

Neuropathologic Characterization of Pontocerebellar Hypoplasia Type 6 Associated With Cardiomyopathy and Hydrops Fetalis and Severe Multisystem Respiratory Chain Deficiency due to Novel RARS2 Mutations

Autosomal recessive mutations in the RARS2 gene encoding the mitochondrial arginyl-transfer RNA synthetase cause infantile-onset myoencephalopathy pontocerebellar hypoplasia type 6 (PCH6). We describe 2 sisters with novel compound heterozygous RARS2 mutations who presented perinatally with neurologic features typical of PCH6 but with additional features including cardiomyopathy, hydrops, and pulmonary hypoplasia and who died at 1 day and 14 days of age. Magnetic resonance imaging findings included marked cerebellar hypoplasia, gyral immaturity, punctate lesions in cerebral white matter, and unfused deep cerebral grey matter. Enzyme histochemistry of postmortem tissues revealed a near-global cytochrome c oxidase-deficiency; assessment of respiratory chain enzyme activities confirmed severe deficiencies involving complexes I, III, and IV. Molecular genetic studies revealed 2 RARS2 gene mutations: a c.1A>G, p.? variant predicted to abolish the initiator methionine, and a deep intronic c.613-3927C>T variant causing skipping of exons 6–8 in the mature RARS2 transcript. Neuropathologic investigation included low brain weights, small brainstem and cerebellum, deep cerebral white matter pathology, pontine nucleus neuron loss (in 1 sibling), and peripheral nerve pathology. Mitochondrial respiratory chain immunohistochemistry in brain tissues confirmed an absence of complexes I and IV immunoreactivity with sparing of mitochondrial numbers. These cases expand the clinical spectrum of RARS2 mutations, including antenatal features and widespread mitochondrial respiratory chain deficiencies in postmortem brain tissues

Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States

Aims: To isolate and characterize fungi associated with diseased soybean seedlings in Midwestern soybean production fields and to determine the influence of environmental and edaphic factors on their incidence. Methods and Results: Seedlings were collected from fields with seedling disease history in 2012 and 2013 for fungal isolation. Environmental and edaphic data associated with each field was collected. 3036 fungal isolates were obtained and assigned to 76 species. The most abundant genera recovered were Fusarium (73%) and Trichoderma (11.2%). Other genera included Mortierella, Clonostachys, Rhizoctonia, Alternaria, Mucor, Phoma, Macrophomina and Phomopsis. Most recovered species are known soybean pathogens. However, non-pathogenic organisms were also isolated. Crop history, soil density, water source, precipitation and temperature were the main factors influencing the abundance of fungal species. Conclusion: Key fungal species associated with soybean seedling diseases occurring in several US production regions were characterized. This work also identified major environment and edaphic factors affecting the abundance and occurrence of these species. Significance and Impact of the Study: The identification and characterization of the main pathogens associated with seedling diseases across major soybean-producing areas could help manage those pathogens, and devise more effective and sustainable practices to reduce the damage they cause

Critical review of behaviour change techniques applied in intervention studies to improve cooking skills and food skills among adults

BACKGROUND: Cooking and food skills interventions have grown in popularity; however, there is a lack of transparency as to how these interventions were designed, highlighting a need to identify and understand the mechanisms of behavior change so that effective components may be introduced in future work. This study critiques cooking and food skills interventions in relation to their design, behavior change techniques (BCTs), theoretical underpinnings, and outcomes. METHODS: A 40-item CALO-RE taxonomy was used to examine the components of 59 cooking and food skills interventions identified by two systematic reviews. Studies were coded by three independent coders. RESULTS: The three most frequently occurring BCTs identified were #1 Provide information on consequences of behavior in general; #21 Provide instruction on how to perform the behavior; and #26 Prompt Practice. Fifty-six interventions reported positive short-term outcomes. Only 14 interventions reported long-term outcomes containing BCTs relating to information provision. CONCLUSION: This study reviewed cooking and food skills interventions highlighting the most commonly used BCTs, and those associated with long-term positive outcomes for cooking skills and diet. This study indicates the potential for using the BCT CALO-RE taxonomy to inform the design, planning, delivery and evaluation of future interventions