Dynamic IMF production in 24Mg+27Al^{24}Mg + ^{27}Al at intermediate energies

The azimuthal correlations and polar-angle distributions of intermediate-mass fragments (IMFs) produced in Mg+Al at 45 an 95 AMeV were studied. Measurements of $\alpha$-particles and IMFs with $3\le Z\le8$ emmitted in the mid-rapidity region for mid-central events were compared to IQMD results and results from a static-source model. A maximum in the azimuthal-correlation function at 180\degree\/ can not be described by independently emmitted particles. Momentum conservation of a small source as well as target-projectile correlations from IQMD show the same azimuthal correlations as the experimental data. The polar-angle distributions in the experimental data show a target-projectile seperation, thus giving evidence of dynamic IMF production.\\ {\it Keywords:} dynamic multifragmentation, IMF, IQMD, azimuthal correlations

Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens

Exosomes are endosome-derived membrane vesicles that contain proteins, lipids, and nucleic acids. The exosomal transcriptome mediates intercellular communication, and represents an understudied reservoir of novel biomarkers for human diseases. Next-generation sequencing enables complex quantitative characterization of exosomal RNAs from diverse sources. However, detailed protocols describing exosome purification for preparation of exosomal RNA-sequence (RNA-Seq) libraries are lacking. Here we compared methods for isolation of exosomes and extraction of exosomal RNA from human cell-free serum, as well as strategies for attaining equal representation of samples within pooled RNA-Seq libraries. We compared commercial precipitation with ultracentrifugation for exosome purification and confirmed the presence of exosomes via both transmission electron microscopy and immunoblotting. Exosomal RNA extraction was compared using four different RNA purification methods. We determined the minimal starting volume of serum required for exosome preparation and showed that high quality exosomal RNA can be isolated from sera stored for over a decade. Finally, RNA-Seq libraries were successfully prepared with exosomal RNAs extracted from human cell-free serum, cataloguing both coding and non-coding exosomal transcripts. This method provides researchers with strategic options to prepare RNA-Seq libraries and compare RNA-Seq data quantitatively from minimal volumes of fresh and archival human cell-free serum for disease biomarker discovery

Clinical evaluation of Corridor disease in Bos indicus (Boran) cattle naturally infected with buffalo-derived Theileria parva

Corridor disease (CD) is a fatal condition of cattle caused by buffalo-derived Theileria parva. Unlike the related condition, East Coast fever, which results from infection with cattle-derived T. parva, CD has not been extensively studied. We describe in detail the clinical and laboratory findings in cattle naturally infected with buffalo-derived T. parva. Forty-six cattle were exposed to buffalo-derived T. parva under field conditions at the Ol Pejeta Conservancy, Kenya, between 2013 and 2018. The first signs of disease observed in all animals were nasal discharge (mean day of onset was 9 days post-exposure), enlarged lymph nodes (10 days post-exposure), and pyrexia (13.7 days post-exposure). Coughing and labored breathing were observed in more than 50% of animals (14 days post-exposure). Less commonly observed signs, corneal edema (22%) and diarrhea (11%), were observed later in the disease progression (19 days post-exposure). All infections were considered clinically severe, and 42 animals succumbed to infection. The mean time to death across all studies was 18.4 days. The mean time from onset of clinical signs to death was 9 days and from pyrexia to death was 4.8 days, indicating a relatively short duration of clinical illness. There were significant relationships between days to death and the days to first temperature (chi2 = 4.00, p = 0.046), and days to peak temperature (chi2 = 25.81, p = 0.001), animals with earlier onset pyrexia died sooner. These clinical indicators may be useful for assessing the severity of disease in the future. All infections were confirmed by the presence of macroschizonts in lymph node biopsies (mean time to parasitosis was 11 days). Piroplasms were detected in the blood of two animals (4%) and 20 (43%) animals seroconverted. In this study, we demonstrate the successful approach to an experimental field study for CD in cattle. We also describe the clinical progression of CD in naturally infected cattle, including the onset and severity of clinical signs and pathology. Laboratory diagnoses based on examination of blood samples are unreliable, and alternatives may not be available to cattle keepers. The rapid development of CD requires recognition of the clinical signs, which may be useful for early diagnosis of the disease and effective intervention for affected animals

The Bulgeless Seyfert/LINER Galaxy NGC 3367: Disk, Bar, Lopsidedness and Environment

NGC3367 is a nearby isolated active galaxy that shows a radio jet, a strong bar and evidence of lopsidedness. We present a quantitative analysis of the stellar and gaseous structure of the galaxy disk and a search for evidence of recent interaction based on new UBVRI Halpha and JHK images and on archival Halpha Fabry-Perot and HI VLA data. From a coupled 1D/2D GALFIT bulge/bar/disk decomposition an (B/D ~ 0.07-0.1) exponential pseudobulge is inferred in all the observed bands. A NIR estimate of the bar strength = 0.44 places NGC 3367 bar among the strongest ones. The asymmetry properties were studied using (1) optical and NIR CAS indexes (2) the stellar (NIR) and gaseous (Halpha, HI) A_1 Fourier mode amplitudes and (3) the HI integrated profile and HI mean intensity distribution. While the average stellar component shows asymmetry values close to the average found in the Local Universe for isolated galaxies, the young stellar component and gas values are largely decoupled showing significantly larger A_1 mode amplitudes suggesting that the gas has been recently perturbed. Our search for (1) faint stellar structures in the outer regions (up to u_R ~ 26 mag arcsec^{-2}), (2) (Halpha) star-forming satellite galaxies and (3) regions with different colors (stellar populations) along the disk all failed. Such an absence is interpreted using recent numerical simulations to constrain a tidal event with an LMC like galaxy to some dynamical times in the past or to a current very low mass, gas rich accretion. We conclude that a cold accretion mode (gas and small/dark galaxies) may be responsible of the nuclear activity and peculiar (young stars and gas) morphology regardless of the highly isolated environment. Black hole growth in bulgeless galaxies may be triggered by cosmic smooth mass accretion.Comment: 27 pages, 12 figures, accepted for publication in The Astronomical Journa

An analytic toy model for relativistic accretion in Kerr spacetime

We present a relativistic model for the stationary axisymmetric accretion flow of a rotating cloud of non-interacting particles falling onto a Kerr black hole. Based on a ballistic approximation, streamlines are described analytically in terms of timelike geodesics, while a simple numerical scheme is introduced for calculating the density field. A novel approach is presented for describing all of the possible types of orbit by means of a single analytic expression. This model is a useful tool for highlighting purely relativistic signatures in the accretion flow dynamics coming from a strong gravitational field with frame-dragging. In particular, we explore the coupling due to this between the spin of the black hole and the angular momentum of the infalling matter. Moreover, we demonstrate how this analytic solution may be used for benchmarking general relativistic numerical hydrodynamics codes by comparing it against results of smoothed particle hydrodynamics simulations for a collapsar-like setup. These simulations are performed first for a ballistic flow (with zero pressure) and then for a hydrodynamical one where we measure the effects of pressure gradients on the infall, thus exploring the extent of applicability of the ballistic approximation.Comment: 15 pages, 8 figures, references and minor changes added to match version accepted for publication in MNRA

Reflecting on loss in Papua New Guinea

This article takes up the conundrum of conducting anthropological fieldwork with people who claim that they have 'lost their culture,' as is the case with Suau people in the Massim region of Papua New Guinea. But rather than claiming culture loss as a process of dispossession, Suau claim it as a consequence of their own attempts to engage with colonial interests. Suau appear to have responded to missionization and their close proximity to the colonial-era capital by jettisoning many of the practices characteristic of Massim societies, now identified as 'kastom.' The rejection of kastom in order to facilitate their relations with Europeans during colonialism, followed by the mourning for kastom after independence, both invite consideration of a kind of reflexivity that requires action based on the presumed perspective of another

Secular Evolution and the Formation of Pseudobulges in Disk Galaxies

We review internal processes of secular evolution in galaxy disks, concentrating on the buildup of dense central features that look like classical, merger-built bulges but that were made slowly out of disk gas. We call these pseudobulges. As an existence proof, we review how bars rearrange disk gas into outer rings, inner rings, and gas dumped into the center. In simulations, this gas reaches high densities that plausibly feed star formation. In the observations, many SB and oval galaxies show central concentrations of gas and star formation. Star formation rates imply plausible pseudobulge growth times of a few billion years. If secular processes built dense central components that masquerade as bulges, can we distinguish them from merger-built bulges? Observations show that pseudobulges retain a memory of their disky origin. They have one or more characteristics of disks: (1) flatter shapes than those of classical bulges, (2) large ratios of ordered to random velocities indicative of disk dynamics, (3) small velocity dispersions, (4) spiral structure or nuclear bars in the bulge part of the light profile, (5) nearly exponential brightness profiles, and (6) starbursts. These structures occur preferentially in barred and oval galaxies in which secular evolution should be rapid. So the cleanest examples of pseudobulges are recognizable. Thus a large variety of observational and theoretical results contribute to a new picture of galaxy evolution that complements hierarchical clustering and merging.Comment: 92 pages, 21 figures in 30 Postscript files; to appear in Annual Review of Astronomy and Astrophysics, Vol. 42, 2004, in press; for a version with full resolution figures, see http://chandra.as.utexas.edu/~kormendy/ar3ss.htm