11 research outputs found

    Antimicrobial Resistance Of Staphylococcus Aureus And Oral Streptococci Strains From High-risk Endocarditis Patients

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    This study sought to determine the antimicrobial susceptibility of Staphylcoccus aureus and viridans group streptococci strains collected from the forearm skin and saliva of 30 patients at high risk of endocarditis. Agar susceptibility tests of antibiotics routinely utilized in dentistry were used to verify antimicrobial resistance of bacterial strains. Of the Staphylcoccus aureus strains, 50% were resistant to ampicillin, 53.3% to amoxicillin, 60.0% to penicillin G, 13.3% to amoxicillin/clavulanate, 20.0% to azithromycin, 27.6% to clarithromycin, 23.3% to erythromycin, 3.3% to cefazolin, and 6.7% to clindamycin. Regarding streptococci, 16.7% of the strains were resistant to ampicillin, 16.7% to amoxicillin, 23.3% to azithromycin, 23.3% to clarithromycin, 30.0% to erythromycin, 13.3% to cefazolin, 26.7% to clindamycin, 16.7% to penicillin G, and 3.3% to amoxicillin/clavulanate. Pathogens associated with bacterial endocarditis exhibited elevated resistance rates against the antibiotics used for prophylaxis in dentistry.536410413Benn, M., Hagelskjaer, L.H., Tvede, M., Infective endocarditis, 1984 through 1993: A clinical and microbiological survey (1997) J Intern Med, 242, pp. 15-22Mansur, A.J., Grinberg, M., Gallucci, S.D., Bellotti, G., Jatene, A., Pileggi, F., Infective endocarditis: Analysis of 300 episodes (1990) Arq Bras Cardiol, 54, pp. 13-21Ruiz Jr., E., Schirmbeck, T., Figueiredo, L.T., A study of infectious endocarditis in Ribeirao Preto, SP-Brazil. Analysis of cases occurring between 1992 and 1997 (2000) Arq Bras Cardiol, 74, pp. 225-231Dajani, A.S., Taubert, K.A., Wilson, W., Bolger, A.F., Bayer, A., Ferrieri, P., Gewitz, M.H., Zuccaro Jr., G., Prevention of bacterial endocarditis. Recommendations by the American Heart Association (1997) Circulation, 96, pp. 358-366Moreillon, P., Que, Y.A., Infective endocarditis (2004) Lancet, 363, pp. 139-149Hogevik, H., Olaison, L., Andersson, R., Lindberg, J., Alestig, K., Epidemiologic aspects of infective endocarditis in an urban population. A 5-year prospective study (1995) Medicine, 74, pp. 324-339. , BaltimoreSandre, R.M., Shafran, S.D., Infective endocarditis: Review of 135 cases over 9 years (1996) Clin Infect Dis, 22, pp. 276-286McCartney, A.C., Changing trends in infective endocarditis (1992) J Clin Pathol, 45, pp. 945-948Carmona, I.T., Diz Dios, P., Scully, C., An update on the controversies in bacterial endocarditis of oral origin (2002) Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 93, pp. 660-670Eykyn, S.J., The treatment of staphylococcal endocarditis (1987) J Antimicrob Chemother, 20 (SUPPL. A), pp. 161-171Petti, C.A., Fowler Jr., V.G., Staphylococcus aureus bacteremia and endocarditis (2002) Infect Dis Clin North Am, 16, pp. 413-435Roman, R.S., Smith, J., Walker, M., Byrne, S., Ramotar, K., Dyck, B., Kabani, A., Nicolle, L.E., Rapid geographic spread of a methicillin-resistant Staphylococcus aureus strain (1997) Clin Infect Dis, 25, pp. 698-705Farias, W.V., Sader, H.S., Lerne, I.L., Pignatari, A.C., Sensitivity pattern of 117 clinical isolates of Staphylococcus aureus from 12 hospitals (1997) Rev Assoc Med Bras, 43, pp. 199-204Perl, T.M., Golub, J.E., New approaches to reduce Staphylococcus aureus nosocomial infection rates: Treating S. aureus nasal carriage (1998) Ann Pharmacother, 32, pp. S7-S16Herzberg, M.C., Meyer, M.W., Effects of oral flora on platelets: Possible consequences in cardiovascular disease (1996) J Periodontol, 67, pp. 1138-1142Baquero, F., Gram-positive resistance: Challenge for the development of new antibiotics (1997) J Antimicrob Chemother, 39 (SUPPL. A), pp. 1-6Martinez, F., Martin-Luengo, F., Garcia, A., Valdes, M., Treatment of experimental endocarditis caused by penicillin-resistant Streptococcus sanguis with different doses of teicoplanin (1994) Methods Find Exp Clin Pharmacol, 16, pp. 247-251Doern, G.V., Ferraro, M.J., Brueggemann, A.B., Ruoff, K.L., Emergence of high rates of antimicrobial resistance among viridans group streptococci in the United States (1996) Antimicrob Agents Chemother, 40, pp. 891-894Levy, C.S., Kogulan, P., Gill, V.J., Croxton, M.B., Kane, J.G., Lucey, D.R., Endocarditis caused by penicillin-resistant viridans streptococci: 2 Cases and controversies in therapy (2001) Clin Infect Dis, 33, pp. 577-579Hall, G.E., Baddour, L.M., Apparent failure of endocarditis prophylaxis caused by penicillin-resistant Streptococcus mitis (2002) Am J Med Sci, 324, pp. 51-53Koneman, E.W., Allen, S.D., Janda, W.M., Schreckenberger, P.C., Winn, W.C., (1994) Introduction to Diagnostic Microbiology, Ed. 1, pp. 187-216. , Philadelphia: Lippincott Williams & Wilkins(2002) Performance Standards for Antimicrobial Susceptibility Testing, 22, pp. 50-69. , Document M100-S12Hoen, B., Platelets and platelet inhibitors in infective endocarditis (2002) Curr Infect Dis Rep, 4, pp. 299-303Tak, T., Reed, K.D., Haselby, R.C., McCauley Jr., C.S., Shukla, S.K., An update on the epidemiology, pathogenesis and management of infective endocarditis with emphasis on Staphylococcus aureus (2002) WMJ, 101, pp. 24-33Moore, P.A., Dental therapeutic indications for the newer long-acting macrolide antibiotics (1999) J Am Dent Assoc, 130, pp. 1341-1343Kohli, V., Infective endocarditis (2002) Indian J Pediatr, 69, pp. 333-339Scavizzi, M.R., Labia, R., Petitjean, O.J., Elbhar, A., Antimicrobial susceptibility test: From bacterial population analysis to therapy (2002) Int J Antimicrob Agents, 19, pp. 9-20Felten, A., Grandry, B., Lagrange, P.H., Casin, I., Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): A disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test (2002) J Clin Microbiol, 40, pp. 2766-2771Grignon, B., Tankovic, J., Megraud, F., Glupczynski, Y., Husson, M.O., Conroy, M.C., Emond, J.P., Fauchere, J.L., Validation of diffusion methods for macrolide susceptibility testing of Helicobacter pylori (2002) Microb Drug Resist, 8, pp. 61-66Chang, S.C., Hsieh, W.C., Liu, C.Y., High prevalence of antibiotic resistance of common pathogenic bacteria in Taiwan (2000) Diag Microbiol Infect Dis, 36, pp. 107-112. , The Antibiotic Resistance Study Group of the Infectious Disease Society of the Republic of ChinaSandor, G.K., Carmichael, R.P., Vasilakos, J.S., Revised American Heart Association guidelines for the prevention of bacterial endocarditis: Highlights of specific changes for the dental profession (1998) J Can Dent Assoc, 64, pp. 114-117Creighton, J.M., Dental care for the pediatric cardiac patient (1992) J Can Dent Assoc, 58, pp. 201-202Steckelberg, J.M., Melton III, L.J., Ilstrup, D.M., Rouse, M.S., Wilson, W.R., Influence of referral bias on the apparent clinical spectrum of infective endocarditis (1990) Am J Med, 88, pp. 582-588Jacobson, J.J., Patel, B., Asher, G., Woolliscroft, J.O., Schaberg, D., Oral staphylococcus in older subjects with rheumatoid arthritis (1997) J Am Geriatr Soc, 45, pp. 590-593Alcaide, F., Linares, J., Pallares, R., Carratala, J., Benitez, M.A., Gudiol, F., Martin, R., In vitro activities of 22 beta-lactam antibiotics against penicillin-resistant and penicillin-susceptible viridans group streptococci isolated from blood (1995) Antimicrob Agents Chemother, 39, pp. 2243-2247Teng, L.J., Hsueh, P.R., Chen, Y.C., Ho, S.W., Luh, K.T., Antimicrobial susceptibility of viridans group streptococci in Taiwan with an emphasis on the high rates of resistance to penicillin and macrolides in Streptococcus oralis (1998) J Antimicrob Chemother, 41, pp. 621-627Chayakul, P., Hortiwakul, R., Yipintsoi, T., Ingviya, N., Viridans streptococci in the oral flora of the patients at risk for infective endocarditis: Species and penicillin susceptibilities (2002) J Med Assoc Thai, 85, pp. 825-830Monroe, S., Polk, R., Antimicrobial use and bacterial resistance (2000) Curr Opin Microbiol, 3, pp. 496-501Seymour, R.A., Whitworth, J.W., Antibiotic prophylaxis for endocarditis, prosthetic joints, and surgery (2002) Dent Clin North Am, 46, pp. 635-65

    Avaliação econômica do confinamento de novilhos de origem leiteira, alimentados com diferentes níveis de concentrado e de cama de frango Economic evaluation of feedlot dairy crossbred bulls fed diets with different concentrate and broiler litter levels

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    Foram confinados 24 bezerros mestiços Holandês x Zebu, machos não-castrados, com peso médio inicial de 75 kg e final de 215 kg, com o objetivo de avaliar o custo de produção. Os animais foram alimentados com capim-elefante de 30 a 45 dias de idade, concentrado à base de farelo de soja, fubá de milho, farinha de carne, mistura mineral e cama de frango, sendo constituídos os tratamentos: 1 = 50% volumoso e 50% concentrado; 2 = 50% volumoso, 35% de concentrado e 15% de cama de frango; 3 = 25% de volumoso e 75% concentrado; e 4 = 25% de volumoso, 52,5% de concentrado e 22,5% de cama de frango, na base da matéria seca. O delineamento experimental foi em blocos casualisados, com seis repetições. A análise da renda bruta, dos custos e do lucro indicou que o lucro foi negativo em todos os tratamentos, quando o preço de venda da carne produzida foi igual ao preço do boi gordo. Entretanto, quando foram mantidos os mesmos custos (custo operacional efetivo e custo operacional total) e elevado em 10% o preço de venda do quilo de carne produzida, em relação ao preço do quilo de carne de boi gordo, todos os tratamentos apresentaram lucro, sendo destacados os tratamentos 2 e 3, com R0,042eR 0,042 e R 0,03 por quilo de carne produzida e retorno sobre capital investido de 14,20 e 14,64%/ano, respectivamente. A variação no preço do concentrado, de R0,26a0,20/kg,causoumaiorimpactonocustooperacionalefetivodotratamento3emenornotratamento2,proporcionandolucroeretornosobrecapitaloinvestidoparatodosostratamentos,apartirdarelac\ca~o7,62:1entreprec\codoconcentradoxprec\codacarne.Assimulac\co~esmostraramqueotratamento2foieconomicamentemaisvantajoso,vistoquegeroumelhorrelac\ca~ocusto/benefıˊcioparaascondic\co~esdopresentetrabalho.<br>TwentyfourHolsteincrossbredyoungbulls,with75kginitialaverageweightand215kgfinalaverageweight,wereconfinedinafeedlot.Theobjectiveofthisexperimentwastoevaluatetheproductioncost.Theanimalswerefedelephantgrasswith30to45daysofage,soybeanmeal,cornmeal,meatmealandmineralmixbasalconcentrate,andbroilerlitter,whichconstitutedthetreatments:1=50 0,26 a 0,20/kg, causou maior impacto no custo operacional efetivo do tratamento 3 e menor no tratamento 2, proporcionando lucro e retorno sobre capital o investido para todos os tratamentos, a partir da relação 7,62:1 entre preço do concentrado x preço da carne. As simulações mostraram que o tratamento 2 foi economicamente mais vantajoso, visto que gerou melhor relação custo/benefício para as condições do presente trabalho.<br>Twenty-four Holstein crossbred young bulls, with 75 kg initial average weight and 215 kg final average weight, were confined in a feedlot. The objective of this experiment was to evaluate the production cost. The animals were fed elephant grass with 30 to 45 days of age, soybean meal, corn meal, meat meal and mineral mix basal concentrate, and broiler litter, which constituted the treatments: 1 = 50% forage and 50% concentrate, 2 = 50% forage, 35% concentrate and 15% broiler litter, 3 = 25% forage and 75% concentrate and 4 = 25% forage, 52.5% concentrate and 22.5% broiler litter, as dry matter basis. It was used the completely randomized blocks experimental design, with six blocks and four treatments. The gross income, costs and profit analysis indicated that the profit was negative in all treatments when the produced meat sale price was the same as the fat ox price. However, when the same costs were maintained (effective operational cost and total operational cost) and the kilo of produced meat sale price rose 10%, relative to the fat ox kilo of meat price, all the treatments presented profit, distinguishing the treatments 2 and 3, with R 0.042 and R0.03perkiloofproducedmeatandreturnontheinvestedcapitalof14.20and14.64 0.03 per kilo of produced meat and return on the invested capital of 14.20 and 14.64%/year, respectively. The concentrate price variation, from 0.26 to 0.20 R/kg, caused larger impact in the effective operational cost of treatment 3 and smaller in treatment 2, providing profit and return on the invested capital for all treatments, based on the 7.62:1 relationship between concentrate price x meat price. The simulations showed that treatment 2 was economically more advantageous because it produced a better cost/benefit relationship in the conditions of the present work

    Boron affects the growth and ultrastructure of castor bean plants Boro afeta o crescimento e a ultra-estrutura da mamoneira

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    The cultivation of oleaginous plants like the castor bean guarantees employment for agricultural families and can contribute in energy and chemical sectors, especially in the northeastern semi-arid regions of Brazil. Boron (B) deficiency is a widespread nutritional disorder despite the fact that various anthropogenic sources with high B content may increase soil B to toxic levels for plants. The present study was designed to investigate the ultrastructural effects of boron deficiency and toxicity on castor bean plants which were grown under greenhouse condition using plastic containers with 10 L of nutrient solution. Boron treatments comprised: control (no B); 0.27 mg L-1, 5.40 mg L-1 B pots (one plant per pot), tested in a completely randomized design with three replicates. The dry matter of all plant parts and B concentration were determined. Cellular ultrastructure was evaluated by transmission and scanning electron microscopy on samples of leaves and petioles. Dry matter yield was affected by the B absence treatment but there was no difference for the 5.4 mg L-1 B (toxic conditions) treatment. A marginal leaf burn at edge and tips of oldest leaves and absence of starch granules in chloroplasts were noted for the B toxicity treatment. The deformation of the youngest leaves, the death of the apical meristem as well as the swelling of the middle lamella, absence of starch granules in chloroplasts and petiole vessels untidily were observed in the B absent treatment. It is concluded that the production and development of castor bean plants is affected by boron deficiency, but not for boron toxicity conditions.<br>A mamoneira é uma oleaginosa com grande potencial para a geração de renda na agricultura familiar e para produção de matéria prima para a indústria química e setor energético brasileiro, especialmente em regiões do semi-árido nordestino. A deficiência de boro (B) ocorre de forma generalizada no Brasil e a aplicação excessiva deste micronutriente pode causar toxicidade. Este estudo avalia o desenvolvimento e os efeitos ultra-estruturais de deficiência e toxicidade de boro em mamoneira. O experimento foi realizado em condições de casa de vegetação, com vasos de 10 L de solução nutritiva. Foram utilizados três tratamentos: testemunha (sem B); 0,27 e 5,40 mg L-1 B, uma planta por vaso, em delineamento experimental inteiramente ao acaso com três repetições. A produção de matéria seca de cada parte da planta foi avaliada e o teor de boro determinado. Foram observados os efeitos morfológicos e as alterações na ultra-estrutura celular nas folhas e pecíolos, através da técnica de microscopia eletrônica de transmissão e varredura. A produção de matéria seca da mamoneira foi afetada em condições de deficiência de boro, mas não em condições de toxicidade (5,4 mg L-1 B). Neste último tratamento foram constatadas cloroses nos bordos de folhas velhas e ausência de grânulos de amido. Na omissão de boro, as plantas apresentaram deformação de folhas novas, morte do meristema apical, engrossamento da lamela média, ausência de grânulos de amido nos cloroplastos e desorganização dos vasos condutores do pecíolo. O desenvolvimento e a produção da mamoneira são afetados em condições de deficiência de boro mas não na condição de toxicidade

    Triangulação entre métodos na administração: gerando conversações paradigmáticas ou meras validações "convergentes"?

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    O objetivo deste artigo é buscar responder à seguinte questão: a triangulação como método tem sido utilizada nas pesquisas em administração de uma perspectiva multiparadigmática, gerando conversações entre paradigmas? Ou tem sido utilizada simplesmente no intuito de se realizar uma validação "convergente" entre os métodos na pesquisa? Para responder à questão, utilizou-se o banco de dados referente a 174 artigos que utilizaram a triangulação em três anais do EnAnpad. Esses artigos foram analisados qualitativamente para verificar de que forma a triangulação foi conduzida, a fim de investigar se a combinação se ateve apenas no nível do método, ou se atingiu o âmbito paradigmático. O posicionamento adotado pelos pesquisadores neste estudo é o de defender a importância da conversação paradigmática para lidar com os fenômenos sociais característicos das pesquisas em administração. Os resultados demonstraram que a combinação de métodos tem sido adotada principalmente no intuito de validação convergente, não implicando necessariamente conversações efetivas entre diferentes visões de mundo e de conhecimento científico. O que se observa é sua aplicação principalmente em estudos nos quais predomina, em termos epistemológicos, uma visão positivista e, em termos metodológicos, uma postura nomotética

    Estimation of biomass and carbon stocks: the case of the Atlantic Forest

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    The main objective of this paper is to present and discuss the best methods to estimate live above ground biomass in the Atlantic Forest. The methods presented and conclusions are the products of a workshop entitled "Estimation of Biomass and Carbon Stocks: the Case of Atlantic Rain Forest". Aboveground biomass (AGB) in tropical forests is mainly contained in trees. Tree biomass is a function of wood volume, obtained from the diameter and height, architecture and wood density (dry weight per unit volume of fresh wood). It can be quantified by the direct (destructive) or indirect method where the biomass quantification is estimated using mathematical models. The allometric model can be site specific when elaborated to a particular ecosystem or general that can be used in different sites. For the Atlantic Forest, despite the importance of it, there are only two direct measurements of tree biomass, resulting in allometric models specific for this ecosystem. To select one or other of the available models in the literature to estimate AGB it is necessary take into account what is the main question to be answered and the ease with which it is possible to measure the independent variables in the model. Models that present more accurate estimates should be preferred. However, more simple models (those with one independent variable, usually DBH) can be used when the focus is monitoring the variation in carbon storage through the time. Our observations in the Atlantic Forest suggest that pan-tropical relations proposed by Chave et al. (2005) can be confidently used to estimated tree biomass across biomes as long as tree diameter (DBH), height, and wood density are accounted for in the model. In Atlantic Forest, we recommend the quantification of biomass of lianas, bamboo, palms, tree ferns and epiphytes, which are an important component in this ecosystem. This paper is an outcome of the workshop entitled "Estimation of Biomass and Carbon Stocks: the Case of Atlantic Rain Forest", that was conducted at Ubatuba, São Paulo, Brazil, between 4 and 8 December 2006 as part of the Brazilian project "Ombrophylus Dense Forest floristic composition, structure and function at the Núcleos Picinguaba and Santa Virginia of the Serra do Mar State Park", BIOTA Gradiente
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