Detection of Non-Technical Losses in Smart Distribution Networks: a Review

With the advent of smart grids, distribution utilities have initiated a large deployment of smart meters on the premises of the consumers. The enormous amount of data obtained from the consumers and communicated to the utility give new perspectives and possibilities for various analytics-based applications. In this paper the current smart metering-based energy-theft detection schemes are reviewed and discussed according to two main distinctive categories: A) system statebased, and B) arti cial intelligence-based.Comisión Europea FP7-PEOPLE-2013-IT

Immature large ribosomal subunits containing the 7S pre-rRNA can engage in translation in Saccharomyces cerevisiae

Evolution has provided eukaryotes with mechanisms that impede immature and/or aberrant ribosomes to engage in translation. These mechanisms basically either prevent the nucleo-cytoplasmic export of these particles or, once in the cytoplasm, the release of associated assembly factors, which interfere with the binding of translation initiation factors and/or the ribosomal subunit joining. We have previously shown that aberrant yeast 40S ribosomal subunits containing the 20S pre-rRNA can engage in translation. In this study, we describe that cells harbouring the dob1–1 allele, encoding a mutated version of the exosome-assisting RNA helicase Mtr4, accumulate otherwise nuclear pre-60S ribosomal particles containing the 7S pre-rRNA in the cytoplasm. Polysome fractionation analyses revealed that these particles are competent for translation and do not induce elongation stalls. This phenomenon is rather specific since most mutations in other exosome components or co-factors, impairing the 3′ end processing of the mature 5.8S rRNA, accumulate 7S pre-rRNAs in the nucleus. In addition, we confirm that pre-60S ribosomal particles containing either 5.8S + 30 or 5.8S + 5 pre-rRNAs also engage in translation elongation. We propose that 7S pre-rRNA processing is not strictly required for pre-60S r-particle export and that, upon arrival in the cytoplasm, there is no specific mechanism to prevent translation by premature pre-60S r-particles containing 3′ extended forms of mature 5.8S rRNA

Influence of size reduction of fly ash particles by grinding on the chemical properties of geopolymers

Chemical properties of geopolymers were evaluated from the reduction of fly ash particle size by grinding. X-ray diffraction determined that at early curing ages new crystalline phases appear in the matrix of the geopolymer and they remain for 28 days, with increases in intensities up to 60%. In Fourier transform infrared spectroscopy, displacements were identified in the main band of the geopolymers at higher wavenumbers, attributed to the greater rigidity in the structures of the aluminosilicate gel due to the increase of the reaction products in the geopolymers obtained through fly ashsubjected to previous grinding, which is observable in the geopolymers matrix. Results indicate that the reduction of fly ash particle size by grinding has an influence on the chemical properties of geopolymers.Peer ReviewedPostprint (published version

JBeaver: un analizador de dependencias para el español

JBeaver es un analizador de dependencias para el español desarrollado utilizando una herramienta de aprendizaje automático (Maltparser ). Este analizador se caracteriza por ser el único públicamente disponible para el español, ser autónomo, fácil de instalar y de utilizar (mediante interfaz gráfica o por comandos de consola) y de elevada precisión. Además, el sistema desarrollado sirve para entrenar de manera sencilla modelos de Maltparser, por lo que se configura en potencia como un analizador de dependencias para cualquier idioma.JBeaver is a dependency parser built using the Maltparser machine-learning tool. It is publically available , easy to install and to use, and provides high precision. It also allows training Maltparser models for any language, so it can be used to train dependency parsers for any language.Partially supported by the Spanish Ministry of Education and Science (TIN2006-14433-C02-01 project)

The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation

Ribosome assembly requires the concerted expression of hundreds of genes, which are transcribed by all three nuclear RNA polymerases. Transcription elongation involves dynamic interactions between RNA polymerases and chromatin. We performed a synthetic lethal screening in Saccharomyces cerevisiae with a conditional allele of SPT6, which encodes one of the factors that facilitates this process. Some of these synthetic mutants corresponded to factors that facilitate pre-rRNA processing and ribosome biogenesis. We found that the in vivo depletion of one of these factors, Arb1, activated transcription elongation in the set of genes involved directly in ribosome assembly. Under these depletion conditions, Spt6 was physically targeted to the upregulated genes, where it helped maintain their chromatin integrity and the synthesis of properly stable mRNAs. The mRNA profiles of a large set of ribosome biogenesismutants confirmed the existence of a feedback regulatory network among ribosome assembly genes. The transcriptional response in this network depended on both the specific malfunction and the role of the regulated gene. In accordance with our screening, Spt6 positively contributed to the optimal operation of this global network. On the whole, this work uncovers a feedback control of ribosome biogenesis by fine-tuning transcription elongation in ribosome assembly factor-coding genes.Ministerio de Economía y Competitividad BFU2013-48643-C3-1-P, BFU2016-77728-C3-1-P, BFU2013-48643-C3- 3-P, BFU2013-42958-PJunta de Andalucía P12-BIO1938MO, P08-CVI-03508Comunidad Valenciana 2015/00

Metodos no invasivos de medición de la inflamación en las neumonitis por hipersensibilidad : utilidad del esputo inducido

El diagnòstic de la pneumonitis per hipersensibilitat requereix en ocasions la realització de tècniques diagnòstiques invasives que de vegades no són possibles de practicar donat el deteriorament de la capacitat funcional que presenta el pacient. L'ús de noves tècniques d'estudi de la inflamació pulmonar, a través de mètodes no invasius, podrien ser d'utilitat en el diagnòstic i seguiment d'aquesta entitat. En el present estudi, plantegem el valor de l'estudi de l'esput induït en el diagnòstic de la pneumonitis per hipersensibilitat, així com la seva possible utilitat en el context de les proves de provocació bronquial específiques.El diagnóstico de la neumonitis por hipersensibilidad requiere en ocasiones la realización de técnicas diagnósticas invasivas que en ocasiones no son posibles de practicar dado el deterioro de la capacidad funcional que presenta el paciente. El empleo de nuevas técnicas de estudio de la inflamación pulmonar, a través de métodos no invasivos, podrían ser de utilidad en el diagnóstico y seguimiento de esta entidad. En el presente estudio, planteamos el valor del estudio del esputo inducido en el diagnóstico de la neumonitis por hipersensibilidad, así como su posible utilidad en el contexto de las pruebas de provocación bronquial específica

The dedicated chaperone Acl4 escorts ribosomal protein Rpl4 to its nuclear pre-60S assembly site

Ribosomes are the highly complex macromolecular assemblies dedicated to the synthesis of all cellular proteins from mRNA templates. The main principles underlying the making of ribosomes are conserved across eukaryotic organisms and this process has been studied in most detail in the yeast Saccharomyces cerevisiae. Yeast ribosomes are composed of four ribosomal RNAs (rRNAs) and 79 ribosomal proteins (r-proteins). Most r-proteins need to be transported from the cytoplasm to the nucleus where they get incorporated into the evolving pre-ribosomal particles. Due to the high abundance and difficult physicochemical properties of r-proteins, their correct folding and fail-safe targeting to the assembly site depends largely on general, as well as highly specialized, chaperone and transport systems. Many r-proteins contain universally conserved or eukaryote-specific internal loops and/or terminal extensions, which were shown to mediate their nuclear targeting and association with dedicated chaperones in a growing number of cases. The 60S r-protein Rpl4 is particularly interesting since it harbours a conserved long internal loop and a prominent C-terminal eukaryote-specific extension. Here we show that both the long internal loop and the C-terminal eukaryote-specific extension are strictly required for the functionality of Rpl4. While Rpl4 contains at least five distinct nuclear localization signals (NLS), the C-terminal part of the long internal loop associates with a specific binding partner, termed Acl4. Absence of Acl4 confers a severe slow-growth phenotype and a deficiency in the production of 60S subunits. Genetic and biochemical evidence indicates that Acl4 can be considered as a dedicated chaperone of Rpl4. Notably, Acl4 localizes to both the cytoplasm and nucleus and it has the capacity to capture nascent Rpl4 in a co-translational manner. Taken together, our findings indicate that the dedicated chaperone Acl4 accompanies Rpl4 from the cytoplasm to its pre-60S assembly site in the nucleus

Synthesis on programmable analog devices from VHDL-AMS

Nowadays, the microelectronics market is characterized by an increasing complexity and integration, in particular in the field of application specific integrated circuits (ASICs) not only for digital but also for mixed-signal designs. The lack of a well defined methodology for analog synthesis, similar to the digital field means a serious drawback for analog and mixed signal design development. In this sense, the arise of VHDL-AMS is a recent evolution which promises to link analog design automation tasks into a coherent framework, in a similar fashion that digital design. In this paper, a tool to perform automated synthesis of analog systems, described in VHDL-AMS, into analog programmable devices is presented. The tool is focused to synthesise filters, wave-shaping circuits, amplifiers and in general most circuits supported by programmable technology. It is demonstrated with a practical example of a analog system composed by two filters and two controllable gain stages.This work has been supported by Ministerio de Ciencia y Tecnología of Spain, under grant TIC2003-09400-C04-02