Comparison of source detection procedures for XMM-Newton images

Procedures based on current methods to detect sources in X-ray images are applied to simulated XMM images. All significant instrumental effects are taken into account, and two kinds of sources are considered -- unresolved sources represented by the telescope PSF and extended ones represented by a b-profile model. Different sets of test cases with controlled and realistic input configurations are constructed in order to analyze the influence of confusion on the source analysis and also to choose the best methods and strategies to resolve the difficulties. In the general case of point-like and extended objects the mixed approach of multiresolution (wavelet) filtering and subsequent detection by SExtractor gives the best results. In ideal cases of isolated sources, flux errors are within 15-20%. The maximum likelihood technique outperforms the others for point-like sources when the PSF model used in the fit is the same as in the images. However, the number of spurious detections is quite large. The classification using the half-light radius and SExtractor stellarity index is succesful in more than 98% of the cases. This suggests that average luminosity clusters of galaxies (L_[2-10] ~ 3x10^{44} erg/s) can be detected at redshifts greater than 1.5 for moderate exposure times in the energy band below 5 keV, provided that there is no confusion or blending by nearby sources. We find also that with the best current available packages, confusion and completeness problems start to appear at fluxes around 6x10^{-16} erg/s/cm^2 in [0.5-2] keV band for XMM deep surveys.Comment: 20 pages, 16 figures. Accepted for publication in A&

Occurrence and distribution of Armillaria gallica genets in a declining oak stand of southern Italy

Outbreaks of Armillaria root rot in conifer plantations and declining oak stands are frequently due to the spread of the fungus in the soil over long periods. This paper examines the occurrence and distribution of Armillaria genets in a declining mixed oak stand of southern Italy. Samples of rhizomorphs, mycelial mats and fruit bodies of Armillaria were collected from the soil, stumps, and living and dead trees. A total of 111 Armillaria isolates were collected, all belonging to the species A. gallica. They were grouped in 28 genets by somatic incompatibility. The largest genet covered an area of about 2.6 ha with a linear extent of 300 m. On the basis of an estimated 0.5 m annual growth in the soil, its age was assumed to be about 3 centuries. The results confirm the ability of A. gallica to remain alive and stable in a large area over a long time

Autopsy in adults with congenital heart disease (ACHD).

The adult congenital heart diseases (ACHD) population is exceeding the pediatric congenital heart diseases (CHD) population and is progressively expanding each year, representing more than 90% of patients with CHD. Of these, about 75% have undergone surgical and/or percutaneous intervention for palliation or correction. Autopsy can be a very challenging procedure in ACHD patients. The approach and protocol to be used may vary depending on whether the pathologists are facing native disease without surgical or percutaneous interventions, but with various degrees of cardiac remodeling, or previously palliated or corrected CHD. Moreover, interventions for the same condition have evolved over the last decades, as has perioperative myocardial preservations and postoperative care, with different long-term sequelae depending on the era in which patients were operated on. Careful clinicopathological correlation is, thus, required to assist the pathologist in performing the autopsy and reaching a diagnosis regarding the cause of death. Due to the heterogeneity of the structural abnormalities, and the wide variety of surgical and interventional procedures, there are no standard methods for dissecting the heart at autopsy. In this paper, we describe the most common types of CHDs that a pathologist could encounter at autopsy, including the various types of surgical and percutaneous procedures and major pathological manifestations. We also propose a practical systematic approach to the autopsy of ACHD patients

Effect of axial coordination on the kinetics of assembly and folding of the two halves of horse heart cytochrome c

The kinetics of the assembly of two complementary fragments of oxidized horse heart cytochrome c (cyt c), namely the heme-containing fragment-(1-56) and the fragment-(57-104), have been characterized at different pH values. At neutral pH the fragment-(1-56) is hexacoordinated and has two histidines axially ligated to the heme-Fe(III) (Santucci, R., Fiorucci, L., Sinibaldi, F., Polizio, F., Desideri, A., and Ascoli, F. (2000) Arch. Biochem. Biophys. 379, 331-336), thus mimicking what occurs in the folding intermediate of cyt c. The kinetics of the formation of the complex between the two fragments are characterized at pH 7.0 by a slow rate constant that is independent of the concentration of the reactants; conversely, at a low pH the kinetics are much faster and depend on the concentration of the fragments. This behavior suggests that the rate-limiting step observed in the recombination process of the fragments at neutral pH (that leads to the final coordination of Met-80) has to be ascribed to the detachment of the "misligated" histidine. Thus, the faster recombination rate at a low pH can be related to the fact that histidine is protonated and not able to coordinate to the metal. Furthermore, the independence of the rate constant on the concentration of the reactants observed at pH 7.0 can be accounted for by the occurrence of a conformational transition, which takes place immediately after the two fragments collapse together, likely simulating what induces the detachment of the misligated histidine during cytochrome folding

Neural stem cell transplantation for neurodegenerative diseases

Neurodegenerative diseases are disabling and fatal neurological disorders that currently lack effective treatment. Neural stem cell (NSC) transplantation has been studied as a potential therapeutic approach and appears to exert a beneficial effect against neurodegeneration via different mechanisms, such as the production of neurotrophic factors, decreased neuroinflammation, enhanced neuronal plasticity and cell replacement. Thus, NSC transplantation may represent an effective therapeutic strategy. To exploit NSCs\u2019 potential, some of their essential biological characteristics must be thoroughly investigated, including the specific markers for NSC subpopulations, to allow profiling and selection. Another key feature is their secretome, which is responsible for the regulation of intercellular communication, neuroprotection, and immunomodulation. In addition, NSCs must properly migrate into the central nervous system (CNS) and integrate into host neuronal circuits, enhancing neuroplasticity. Understanding and modulating these aspects can allow us to further exploit the therapeutic potential of NSCs. Recent progress in gene editing and cellular engineering techniques has opened up the possibility of modifying NSCs to express select candidate molecules to further enhance their therapeutic effects. This review summarizes current knowledge regarding these aspects, promoting the development of stem cell therapies that could be applied safely and effectively in clinical settings

DNA-binding protects p53 from interactions with cofactors involved in transcription-independent functions

Binding-induced conformational changes of a protein at regions distant from the binding site may play crucial roles in protein function and regulation. The p53 tumour suppressor is an example of such an allosterically regulated protein. Little is known, however, about how DNA binding can affect distal sites for transcription factors. Furthermore, the molecular details of how a local perturbation is transmitted through a protein structure are generally elusive and occur on timescales hard to explore by simulations. Thus, we employed state-of-the-art enhanced sampling atomistic simulations to unveil DNA-induced effects on p53 structure and dynamics that modulate the recruitment of cofactors and the impact of phosphorylation at Ser215. We show that DNA interaction promotes a conformational change in a region 3 nm away from the DNA binding site. Specifically, binding to DNA increases the population of an occluded minor state at this distal site by more than 4-fold, whereas phosphorylation traps the protein in its major state. In the minor conformation, the interface of p53 that binds biological partners related to p53 transcription-independent functions is not accessible. Significantly, our study reveals a mechanism of DNA-mediated protection of p53 from interactions with partners involved in the p53 transcription-independent signalling. This also suggests that conformational dynamics is tightly related to p53 signalling

The Concept of “Protected Trade Dispute” in the UK Legislation: A (Still and Never-Ending) Fashionable Notion to be Exported to the Continent, Despite “Metrobus” and “British Airways”?!

Although the right to strike is increasingly “populated” by rulings issued by the “international courts” (European Court of Justice; European Court of Human Rights) and doctrinally wielded with passion, particularly in the “Continent”, there might be some reasons to think and affirm that the “Thatcherite” legacy of the immunity from the strike “made in Britain”, enshrined in the relevant 1992 statute and left basically untouched in the ensuing “Blairite” era, is still both valid domestically and viable as a paradigm for legislations across the Channel. It is déjà vu to read among British scholars that the model of the TULR(C)A is too rigid, particularly in looking at some detailed procedures (eg balloting) which are required to be complied with, for the strike to be legitimately acted upon. However, as this legal analysis of a comparative nature manages hopefully to corroborate, an alternate model of industrial action, particularly the Italian sciopero (strike), inspired to a totally different philosophy (loose legislation and therefore ample power conferred on trade unions) and affected by so many legislative inconsistencies, might suggest the opposite, particularly in light of the serious problems of industrial productivity affecting that country. The British entrenched approach to industrial action, if looked at from this perspective and despite some recent judicial blunders (the “Metrobus” case), may well be a “family jewel” not to nonchalantly dismiss as, save for some minor flaws which are emphasised in this work, no one in Britain is keen to jump from the “frying pan” into the “fire”

RNS60 exerts therapeutic effects in the SOD1 ALS mouse model through protective glia and peripheral nerve rescue

Background: Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease that affects the motor neuromuscular system leading to complete paralysis and premature death. The multifactorial nature of ALS that involves both cell-autonomous and non-cell-autonomous processes contributes to the lack of effective therapies, usually targeted to a single pathogenic mechanism. RNS60, an experimental drug containing oxygenated nanobubbles generated by modified Taylor-Couette-Poiseuille flow with elevated oxygen pressure, has shown anti-inflammatory and neuroprotective properties in different experimental paradigms. Since RNS60 interferes with multiple cellular mechanisms known to be involved in ALS pathology, we evaluated its effect in in vitro and in vivo models of ALS. Methods: Co-cultures of primary microglia/spinal neurons exposed to LPS and astrocytes/spinal neurons from SOD1 G93A mice were used to examine the effect of RNS60 or normal saline (NS) on the selective motor neuron degeneration. Transgenic SOD1 G93A mice were treated with RNS60 or NS (300\u3bcl/mouse intraperitoneally every other day) starting at the disease onset and examined for disease progression as well as pathological and biochemical alterations. Results: RNS60 protected motor neurons in in vitro paradigms and slowed the disease progression of C57BL/6-SOD1 G93A mice through a significant protection of spinal motor neurons and neuromuscular junctions. This was mediated by the (i) activation of an antioxidant response and generation of an anti-inflammatory environment in the spinal cord; (ii) activation of the PI3K-Akt pro-survival pathway in the spinal cord and sciatic nerves; (iii) reduced demyelination of the sciatic nerves; and (iv) elevation of peripheral CD4+/Foxp3+ T regulatory cell numbers. RNS60 did not show the same effects in 129Sv-SOD1 G93A mice, which are unable to activate a protective immune response. Conclusion: RNS60 demonstrated significant therapeutic efficacy in C57BL/6-SOD1 G93A mice by virtue of its effects on multiple disease mechanisms in motor neurons, glial cells, and peripheral immune cells. These findings, together with the excellent clinical safety profile, make RNS60 a promising candidate for ALS therapy and support further studies to unravel its molecular mechanism of action. In addition, the differences in efficacy of RNS60 in SOD1 G93A mice of different strains may be relevant for identifying potential markers to predict efficacy in clinical trials

High resolution radio continuum survey of M33: I. The radio maps

We study the exponential scale length of total radio emission, the spectral index distribution, and the linear radio polarization in the Scd galaxy M33. Observations were carried out using the 3.6 cm dual channel and the 6.2 cm four channel receivers of the 100--m Effelsberg telescope along with the L-band VLA D--array at 20 cm. High spatial resolution and sensitivity in both total and linearly polarized radio continuum emission from M33 were achieved. We found considerable extended emission, not only from the main arms I S and I N, but also from the weaker arms. The large--scale magnetic field exhibits well--ordered spiral structure with almost the same orientation as that of the optical spiral arms, however, it does not show a clear structural correlation or anti--correlation with the optical arms. There is a north-south asymmetry in polarization that is frequency-dependent. We found that the ring mean spectral index versus radius increases faster beyond $R$ = 4 kpc. At each wavelength, the exponential scale length is larger inside than outside $R$ = 4 kpc. From the larger scales lengths at $R$ $<$ 4 kpc, we conclude that star forming regions are mainly spread over the region $R$ $<$ 4 kpc without a dominant nuclear concentration. Furthermore, at $R$ $<$ 4 kpc, a spatial correlation between cosmic rays and star forming regions may exist. From the behaviour of the mean spectral indices obtained from different pairs of the radio continuum data at 3.6, 6.2, and 20 cm, we confirm that a decrease in the thermal fraction causes an increase in the spectral index. The frequency-dependent asymmetry in the polarization hints to an asymmetry in Faraday depolarization.Comment: 16 pages, 13 figures, accepted for publication in the Astronomy and Astrophysics Journa