23,398 research outputs found
Structure-mechanics relationships of collagen fibrils in the Osteogenesis Imperfecta Mouse model
The collagen molecule, which is the building block of collagen fibrils, is a triple helix of two α1(I) chains and one α2(I) chain. However, in the severe mouse model of osteogenesis imperfecta (OIM), deletion of the COL1A2 gene results in the substitution of the α2(I) chain by one α1(I) chain. As this substitution severely impairs the structure and mechanics of collagen-rich tissues at the tissue and organ level, the main aim of this study was to investigate how the structure and mechanics are altered in OIM collagen fibrils. Comparing results from atomic force microscopy imaging and cantilever-based nanoindentation on collagen fibrils from OIM and wild-type (WT) animals, we found a 33% lower indentation modulus in OIM when air-dried (bound water present) and an almost fivefold higher indentation modulus in OIM collagen fibrils when fully hydrated (bound and unbound water present) in phosphate-buffered saline solution (PBS) compared with WT collagen fibrils. These mechanical changes were accompanied by an impaired swelling upon hydration within PBS. Our experimental and atomistic simulation results show how the structure and mechanics are altered at the individual collagen fibril level as a result of collagen gene mutation in OIM. We envisage that the combination of experimental and modelling approaches could allow mechanical phenotyping at the collagen fibril level of virtually any alteration of collagen structure or chemistry.United States. Dept. of Defense. Presidential Early Career Award for Scientists and EngineersNational Science Foundation (U.S.) (CAREER Award
Modelling the overdiagnosis of breast cancer due to mammography screening in women aged 40 to 49 in the United Kingdom
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, andreproduction in any
medium, provided the original work is properly cited
An Optimal Algorithm for Tiling the Plane with a Translated Polyomino
We give a -time algorithm for determining whether translations of a
polyomino with edges can tile the plane. The algorithm is also a
-time algorithm for enumerating all such tilings that are also regular,
and we prove that at most such tilings exist.Comment: In proceedings of ISAAC 201
The Portrayal of Obesity in UK National Newspapers
Obesity is a major medical health challenge; however, the associated stigma represents a harmful and obstructive obstacle to treatment. The study purpose was to explore the portrayal of obesity in UK national newspapers. A purposive sample of 312 editions (weekday: n = 208; weekend: n = 104) from six newspapers (3 broadsheet; 3 tabloid) was analyzed. All text related to obesity was content analyzed. In total 349 articles were analyzed. Weight stigmatization and the portrayal of overweight and obesity as a moral digression were evident. Findings demonstrate that UK national newspapers attribute obesity to controllable causes. Caricatured portrayals of overweight and obesity was evident and may play a pivotal role in the formation and maintenance of anti-obesity attitudes
The temperature and entropy of CFT on time-dependent backgrounds
We express the AdS-Schwarzschild black-hole configuration in coordinates such
that the boundary metric is of the FLRW type. We review how this construction
can be used in order to calculate the stress-energy tensor of the dual CFT on
the FLRW background. We deduce the temperature and entropy of the CFT, which
are related to the temperature and entropy of the black hole. We find that the
entropy is proportional to the area of an apparent horizon, different from the
black-hole event horizon. For a dS boundary we reproduce correctly the
intrinsic temperature of dS space.Comment: 19 pages, major revision, several comments added, version to appear
in JHE
Chaos in the Gauge/Gravity Correspondence
We study the motion of a string in the background of the Schwarzschild black
hole in AdS_5 by applying the standard arsenal of dynamical systems. Our
description of the phase space includes: the power spectrum, the largest
Lyapunov exponent, Poincare sections and basins of attractions. We find
convincing evidence that the motion is chaotic. We discuss the implications of
some of the quantities associated with chaotic systems for aspects of the
gauge/gravity correspondence. In particular, we suggest some potential
relevance for the information loss paradox.Comment: 29 pages, 11 figure
Recommended from our members
Binding of thrombin to glycoprotein Ib accelerates the hydrolysis of Par-1 on intact platelets
The activation of human platelets by α-thrombin is mediated at least in part by cleavage of protease-activated G-protein-coupled receptors, PAR-1 and PAR-4. Platelet glycoprotein Ibα also has a high affinity binding site for α-thrombin, and this interaction contributes to platelet activation through a still unknown mechanism. In the present study the hypothesis that GpIbα may contribute to platelet activation by modulating the hydrolysis of PAR-1 on the platelet membrane was investigated. Gel-filtered platelets from normal individuals were stimulated by α-thrombin, and the kinetics of PAR-1 hydrolysis by enzyme was followed with flow cytometry using an anti-PAR-1 monoclonal antibody (SPAN 12) that recognizes only intact PAR-1 molecules. This strategy allowed measurement of the apparentk cat/K m value for thrombin hydrolysis of PAR-1 on intact platelets, which was equal to 1.5 ± 0.1 × 107 m −1sec−1. The hydrolysis rate of PAR-1 by thrombin was measured under conditions in which thrombin binding to GpIb was inhibited by different strategies, with the following results. 1) Elimination of GpIbα on platelet membranes by mocarhagin treatment reduced the k cat/K m value by about 6-fold. 2) A monoclonal anti-GpIb antibody reduced the apparent k cat/K m value by about 5-fold. 3) An oligonucleotide DNA aptamer, HD22, which binds to the thrombin heparin-binding site (HBS) and inhibits thrombin interaction with GpIbα, reduced the apparentk cat/K m value by about 5-fold. 4) Displacement of α-thrombin from the binding site on GpIb using PPACK-thrombin reduced the apparentk cat/K m value by about 5-fold, and 5) mutation at the HBS of thrombin (R98A) caused a 5-fold reduction of the apparentk cat/K m value of PAR-1 hydrolysis. Altogether these results show that thrombin interaction with GpIb enhances the specificity of thrombin cleavage of PAR-1 on intact platelets, suggesting that GpIb may function as a “cofactor” for PAR-1 activation by thrombin
Black Hole Emission in String Theory and the String Phase of Black Holes
String theory properly describes black-hole evaporation. The quantum string
emission by Black Holes is computed. The black-hole temperature is the Hawking
temperature in the semiclassical quantum field theory (QFT) regime and becomes
the intrinsic string temperature, T_s, in the quantum (last stage) string
regime. The QFT-Hawking temperature T_H is upper bounded by the string
temperature T_S. The black hole emission spectrum is an incomplete gamma
function of (T_H - T_S). For T_H << T_S, it yields the QFT-Hawking emission.
For T_H \to T_S, it shows highly massive string states dominate the emission
and undergo a typical string phase transition to a microscopic `minimal' black
hole of mass M_{\min} or radius r_{\min} (inversely proportional to T_S) and
string temperature T_S. The string back reaction effect (selfconsistent black
hole solution of the semiclassical Einstein equations) is computed. Both, the
QFT and string black hole regimes are well defined and bounded.The string
`minimal' black hole has a life time tau_{min} simeq (k_B c)/(G hbar [T_S]^3).
The semiclassical QFT black hole (of mass M and temperature T_H) and the string
black hole (of mass M_{min} and temperature T_S) are mapped one into another by
a `Dual' transform which links classical/QFT and quantum string regimes.Comment: LaTex, 22 pages, Lectures delivered at the Chalonge School, Nato ASI:
Phase Transitions in the Early Universe: Theory and Observations. To appear
in the Proceedings, Editors H. J. de Vega, I. Khalatnikov, N. Sanchez.
(Kluwer Pub
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