Consumer Perception of Sorghum Variety Attributes in the Lake Zone Tanzania

Many sorghum varieties have been developed by research institutes in an effort to address food security problems in the semi-arid areas of Tanzania. Although sorghum is better adapted to drier areas than maize, farmer adoption rates for sorghum varieties are always lower than that of maize. In addition, maize based food is more acceptable to urban consumers than sorghum based food. In this study consumer evaluated quality attributes of sorghum ugali based on different varieties in order to determine marketing potential relating to the different improved sorghum varieties. A total of 231 consumers, randomly selected from urban and rural areas participated in a food panel to evaluate ugali prepared from five sorghum varieties (three improved, two local). Conjoint analysis was used to determine consumer perceptions of the variety attributes, while a logistic model was applied to determine preference ranking of different varieties. The results indicated that the color and taste of sorghum ugali were the most important criteria used by consumers to evaluate the quality ugali. The study results indicated that sorghum ugali with white/khaki color and the majority of panel participants preferred neutral or slightly sweet taste. Consumers from rural and urban areas accepted two improved varieties; only consumers from rural areas accepted the remaining variety.Adoption, consumer perception, conjoint analysis, sorghum varieties, Tanzania, Agricultural and Food Policy, Consumer/Household Economics, Demand and Price Analysis, Food Consumption/Nutrition/Food Safety, Food Security and Poverty, Marketing, Productivity Analysis, Research and Development/Tech Change/Emerging Technologies,

Water cluster calibration reduces mass error in electrospray ionization mass spectrometry of proteins

Herein we report a novel calibration routine for use in positive ion mode electrospray ionization mass spectrometry (ESI-MS). Monoisotopic masses were calculated for a series of water clusters and used as calibration reference files (available at ). The water cluster series contains singly charged peaks every 18 Da, which allows calibration curves to be precisely defined over a broad mass-to-charge ratio range. Water clusters, induced by a combination of high flow rate and high cone voltage, were used to accurately calibrate a quadrupole mass spectrometer from 100 to 1900 m/z. Calibration curves thus generated have many more data points and greatly reduced standard deviations compared to those obtained from myoglobin, sodium iodide, cesium iodide, or poly(ethylene glycol) based calibration standards. This calibration routine reduces the error in protein mass measurements by a factor of 3, from ±0.01% to ±0.0035% at the 95% confidence limit. The implications of this increased mass accuracy and wider calibrated mass-to-charge ratio scale for the study of protein sequence, structure, and folding by ESI-MS are discussed

Stratospheric Data Analysis System (STRATAN)

A state of the art stratospheric analyses using a coupled stratosphere/troposphere data assimilation system is produced. These analyses can be applied to stratospheric studies of all types. Of importance to this effort is the application of the Stratospheric Data Analysis System (STRATAN) to constituent transport and chemistry problems

Exploitation of Latex Rubber

All of this recent work seems to be affected by two common concerns. The first might be called a work attitude or procedural rule. It requires that I not completely know the final result of any one piece as I start it, that I allow a good deal of the outcome to be the result of an investigation into the working qualities of the material. This attitude was established by my early experiences with clay and predates any conceptual intention; which is the other common concern. By exploiting the procedural nuances of my materials and working process, it is possible for me to encounter the unexpected and exciting while serving the function of attaching some metaphoric identities to my forms that deal with image making and association. &nbsp;</p

Fluorescent visualization of a spreading surfactant

The spreading of surfactants on thin films is an industrially and medically important phenomenon, but the dynamics are highly nonlinear and visualization of the surfactant dynamics has been a long-standing experimental challenge. We perform the first quantitative, spatiotemporally-resolved measurements of the spreading of an insoluble surfactant on a thin fluid layer. During the spreading process, we directly observe both the radial height profile of the spreading droplet and the spatial distribution of the fluorescently-tagged surfactant. We find that the leading edge of spreading circular layer of surfactant forms a Marangoni ridge in the underlying fluid, with a trough trailing the ridge as expected. However, several novel features are observed using the fluorescence technique, including a peak in the surfactant concentration which trails the leading edge, and a flat, monolayer-scale spreading film which differs from concentration profiles predicted by current models. Both the Marangoni ridge and surfactant leading edge can be described to spread as $R \propto t^{\delta}$. We find spreading exponents, $\delta_H \approx 0.30$ and $\delta_\Gamma \approx 0.22$ for the ridge peak and surfactant leading edge, respectively, which are in good agreement with theoretical predictions of $\delta = 1/4$. In addition, we observe that the surfactant leading edge initially leads the peak of the Marangoni ridge, with the peak later catching up to the leading edge

Liquid crystal lens with large focal length tunability and low operating voltage

We demonstrate a tunable focus liquid crystal (LC) lens by sandwiching a homogeneous LC layer between a planar electrode and a curved electrode. The curved electrode which is made of conductive polymer has parabolic shape with a large apex distance. Such design dramatically reduces the phase loss which leads to a short focal length (similar to 15 cm). By using a thin top glass substrate on the curved electrode side, the operating voltage of the lens cell for achieving the shortest focal length is reduced to similar to 23 V-rms. This LC lens has advantages in large focal length tunability, low operating voltage, and good mechanical stability

Mutations in the E2 glycoprotein and the 3\u27 untranslated region enhance chikungunya virus virulence in mice

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes debilitating musculoskeletal pain and inflammation and can persist for months to years after acute infection. Although studies of humans and experimentally infected animals suggest that CHIKV infection persists in musculoskeletal tissues, the mechanisms for this remain poorly understood. To evaluate this further, we isolated CHIKV from the serum of persistently infected Rag1 -/- mice at day 28. When inoculated into naive wild-type (WT) mice, this persistently circulating CHIKV strain displayed a capacity for earlier dissemination and greater pathogenicity than the parental virus. Sequence analysis revealed a nonsynonymous mutation in the E2 glycoprotein (E2 K200R) and a deletion within the 3' untranslated region (3'-UTR). The introduction of these changes into the parental virus conferred enhanced virulence in mice, although primary tropism for musculoskeletal tissues was maintained. The E2 K200R mutation was largely responsible for enhanced viral dissemination and pathogenicity, although these effects were augmented by the 3'- UTR deletion. Finally, studies with Irf3/Irf7 -/- and Ifnar1 -/- mice suggest that the E2 K200R mutation enhances viral dissemination from the site of inoculation independently of interferon regulatory factor 3 (IRF3)-, IRF7-, and IFNAR1-mediated responses. As our findings reveal viral determinants of CHIKV dissemination and pathogenicity, their further study should help to elucidate host-virus interactions that determine acute and chronic CHIKV infection

Synchronous dynamics of zooplankton competitors prevail in temperate lake ecosystems

Although competing species are expected to exhibit compensatory dynamics (negative temporal covariation), empirical work has demonstrated that competitive communities often exhibit synchronous dynamics (positive temporal covariation). This has led to the suggestion that environmental forcing dominates species dynamics; however, synchronous and compensatory dynamics may appear at different length scales and/or at different times, making it challenging to identify their relative importance. We compiled 58 long-term datasets of zooplankton abundance in north-temperate and sub-tropical lakes and used wavelet analysis to quantify general patterns in the times and scales at which synchronous/compensatory dynamics dominated zooplankton communities in different regions and across the entire dataset. Synchronous dynamics were far more prevalent at all scales and times and were ubiquitous at the annual scale. Although we found compensatory dynamics in approximately 14% of all combinations of time period/scale/lake, there were no consistent scales or time periods during which compensatory dynamics were apparent across different regions. Our results suggest that the processes driving compensatory dynamics may be local in their extent, while those generating synchronous dynamics operate at much larger scales. This highlights an important gap in our understanding of the interaction between environmental and biotic forces that structure communities

The Rate and Effects of Spontaneous Mutation on Fitness Traits in the Social Amoeba, Dictyostelium discoideum

We performed a mutation accumulation (MA) experiment in the social amoeba Dictyostelium discoideum to estimate the rate and distribution of effects of spontaneous mutations affecting eight putative fitness traits. We found that the per-generation mutation rate for most fitness components is 0.0019 mutations per haploid genome per generation or larger. This rate is an order of magnitude higher than estimates for fitness components in the unicellular eukaryote Saccharomyces cerevisiae, even though the base-pair substitution rate is two orders of magnitude lower. The high rate of fitness-altering mutations observed in this species may be partially explained by a large mutational target relative to S. cerevisiae. Fitness-altering mutations also may occur primarily at simple sequence repeats, which are common throughout the genome, including in coding regions, and may represent a target that is particularly likely to give fitness effects upon mutation. The majority of mutations had deleterious effects on fitness, but there was evidence for a substantial fraction, up to 40%, being beneficial for some of the putative fitness traits. Competitive ability within the multicellular slug appears to be under weak directional selection, perhaps reflecting the fact that slugs are sometimes, but not often, comprised of multiple clones in nature. Evidence for pleiotropy among fitness components across MA lines was absent, suggesting that mutations tend to act on single fitness components