16 research outputs found
Infrared Observations During the Secondary Eclipse of HD 209458b: I. 3.6-Micron Occultation Spectroscopy Using the VLT
We search for an infrared signature of the transiting extrasolar planet HD
209458b during secondary eclipse. Our method, which we call `occultation
spectroscopy,' searches for the disappearance and reappearance of weak spectral
features due to the exoplanet as it passes behind the star and later reappears.
We argue that at the longest infrared wavelengths, this technique becomes
preferable to conventional `transit spectroscopy'. We observed the system in
the wing of the strong nu-3 band of methane near 3.6 microns during two
secondary eclipses, using the VLT/ISAAC spectrometer at a spectral resolution
of 3300. Our analysis, which utilizes a model template spectrum, achieves
sufficient precision to expect detection of the spectral structure predicted by
an irradiated, low-opacity (cloudless), low-albedo, thermochemical equilibrium
model for the exoplanet atmosphere. However, our observations show no evidence
for the presence of this spectrum from the exoplanet, with the statistical
significance of the non-detection depending on the timing of the secondary
eclipse, which depends on the assumed value for the orbital eccentricity. Our
results reject certain specific models of the atmosphere of HD 209458b as
inconsistent with our observations at the 3-sigma level, given assumptions
about the stellar and planetary parameters.Comment: 26 pages, 8 figures Accepted to Astrophysical Journa
Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio
Bdellovibrio bacteriovorus invade Gram-negative bacteria in a predatory process requiring Type IV pili (T4P) at a single invasive pole, and also glide on surfaces to locate prey. Ras-like G-protein MglA, working with MglB and RomR in the deltaproteobacterium Myxococcus xanthus, regulates adventurous gliding and T4P-mediated social motility at both M. xanthus cell poles. Our bioinformatic analyses suggested that the GTPase activating protein (GAP)-encoding gene mglB was lost in Bdellovibrio, but critical residues for MglABd GTP-binding are conserved. Deletion of mglABd abolished prey-invasion, but not gliding, and reduced T4P formation. MglABd interacted with a previously uncharacterised tetratricopeptide repeat (TPR) domain protein Bd2492, which we show localises at the single invasive pole and is required for predation. Bd2492 and RomR also interacted with cyclic-di-GMP-binding receptor CdgA, required for rapid prey-invasion. Bd2492, RomRBd and CdgA localize to the invasive pole and may facilitate MglA-docking. Bd2492 was encoded from an operon encoding a TamAB-like secretion system. The TamA protein and RomR were found, by gene deletion tests, to be essential for viability in both predatory and non-predatory modes. Control proteins, which regulate bipolar T4P-mediated social motility in swarming groups of deltaproteobacteria, have adapted in evolution to regulate the anti-social process of unipolar prey-invasion in the âlone-hunterâ Bdellovibrio. Thus GTP-binding proteins and cyclic-di-GMP inputs combine at a regulatory hub, turning on prey-invasion and allowing invasion and killing of bacterial pathogens and consequent predatory growth of Bdellovibrio
Activity of Bdellovibrio Hit Locus Proteins, Bd0108 and Bd0109, Links Type IVa Pilus Extrusion/Retraction Status to Prey-Independent Growth Signalling
Bdellovibrio bacteriovorus are facultatively predatory bacteria that grow within gram-negative prey, using pili to
invade their periplasmic niche. They also grow prey-independently on organic nutrients after undergoing a reversible
switch. The nature of the growth switching mechanism has been elusive, but several independent reports suggested
mutations in the hit (host-interaction) locus on the Bdellovibrio genome were associated with the transition to preyindependent
growth. Pili are essential for prey entry by Bdellovibrio and sequence analysis of the hit locus predicted
that it was part of a cluster of Type IVb pilus-associated genes, containing bd0108 and bd0109. In this study we have
deleted the whole bd0108 gene, which is unique to Bdellovibrio, and compared its phenotype to strains containing
spontaneous mutations in bd0108 and the common natural 42 bp deletion variant of bd0108. We find that deletion of
the whole bd0108 gene greatly reduced the extrusion of pili, whereas the 42 bp deletion caused greater pilus
extrusion than wild-type. The pili isolated from these strains were comprised of the Type IVa pilin protein; PilA.
Attempts to similarly delete gene bd0109, which like bd0108 encodes a periplasmic/secreted protein, were not
successful, suggesting that it is likely to be essential for Bdellovibrio viability in any growth mode. Bd0109 has a
sugar binding YD- repeat motif and an N-terminus with a putative pilin-like fold and was found to interact directly with
Bd0108. These results lead us to propose that the Bd0109/Bd0108 interaction regulates pilus production in
Bdellovibrio (possibly by interaction with the pilus fibre at the cell wall), and that the presence (and possibly retraction
state) of the pilus feeds back to alter the growth state of the Bdellovibrio cell. We further identify a novel small RNA
encoded by the hit locus, the transcription of which is altered in different bd0108 mutation background
Tunable far infrared laser spectroscopy of a ternary van der Waals cluster Ar2HCl: A sensitive probe of three-body forces
The first far infrared intermolecular vibrationârotation spectrum of the ternary van der Waals cluster has been measured near 39.5 cm-1 and assigned to an a-type [Sum] bending vibration of Ar2HCl. Spectra of both chlorine isotopes were observed and nuclear quadrupole hyperfine structure was resolved. Values of the fitted constants (rotational constants, hyperfine projections) evidence large amplitude out-of-plane motion, and demonstrate the sensitivity of spectroscopic observables to the three body forces operative in the Ar2HCl system. Spectroscopic predictions calculated by Hutson et al. from pairwise-additive and ââthree-bodyââ corrected potential energy surfaces [J. Chem. Phys. 90, 1337 (1989)] are compared to experimental results
Far-infrared laser vibrationârotationâtunneling spectroscopy of the propaneâwater complex: Torsional dynamics of the hydrogen bond
The far-infrared laser vibrationârotationâtunneling (FIR-VRT) spectrum of the propaneâwater complex has been measured in the range 18â22 cm-1. A C-type VRT band has been assigned with a band origin of 19.6 cm-1. The data support the ââkite-shapedââ structure determined from microwave spectroscopy in the accompanying paper, and indicate that the observed VRT band corresponds to torsional motion of the free water proton about the hydrogen bond. This motion is impeded by a barrier that is less than 5 cm-1. We describe our modification of the supersonic slit-jet source designed to permit Stark effects to be measured, and have used second-order Stark shifts to help assign the perpendicular transition observed
An investigation of three-body effects in intermolecular forces II: Far-infrared vibrationârotationâtunneling laser spectroscopy of Ar2HCl
A second Ar2HCl intermolecular vibrationârotation band centered at 37.2 cm-1 has been measured and assigned as a b-type transition originating from the ground state. Nuclear hyperfine splittings were resolved for both chlorine isotopes. The rotational constants determined from the data indicate coupling between an ArâAr stretching or bending coordinate and the Ar2 âHCl vibrational coordinates. As a result of this particular vibrational motion, Ar2H 35Cl undergoes an axis-switching transition while the Ar2H 37Cl isotope does not. In addition, the measured hyperfine projections indicate the possibility of coupling between the Ar2 âHCl stretching and bending modes, preventing an absolute vibrational assignment. These results indicate that the ââreversed adiabaticââ approximation employed by Hutson, Beswick, and Halberstadt in their theoretical study of Ar2HCl [J. Chem. Phys. 90, 1337 (1989)] is not appropriate for the complicated intramolecular dynamics presently observed in this system
Far-infrared vibration-rotation-tunnelling spectroscopy of ArDCl: A critical test of the H6(4, 3, 0) potential surface
Three intermolecular vibrations of the ArDCl complex have been observed by tunable far-infrared laser spectroscopy in a supersonic planar jet. Vibration-rotation-tunnelling transitions to states correlating to the j = 1, Î = 0, the j = 2, Î = 0, and the j = 2, Î = 1 internal rotor levels of DCl have been measured for both chlorine isotopes with nuclear quadrupole hyperfine resolution. The fitted spectroscopic constants are compared with recent calculations from the new H6(4, 3, 0) ArHCl potential by Hutson and it is concluded that, although the new potential is very accurate, significant discrepancies between observed and calculated values exist for states which probe the secondary minimum (ArClH) of the potential
Fourier transform microwave spectrum of the propaneâwater complex: A prototypical water-hydrophobe system
The Fourier transform microwave spectrum of the propaneâwater complex (C3H8âH2O) has been observed and analyzed. This spectrum includes transitions assigned to propane complexed with both the ortho and para nuclear spin confirmations of water. The rotational constants indicate that the vibrationally averaged structure has all four heavy atoms coplanar, with the water center of mass lying on or near the C2 axis of propane, inside the CCC angle, 3.76(±0.02) A-ring from the propane center-of-mass, and 4.35(±0.02) A-ring from the methylene carbon. The projection of the electric dipole onto the a inertial axis of the complex (0.732 D for the ortho state and 0.819 D for the para state) indicates that one of the protons of the water subunit lies on the C2 axis of the propane monomer, which is also the axis connecting the subunit centers of mass. The small projection of the dipole along the b axis (0.14 D for the ortho state and 0.38 D for the para state) is most consistent with an equilibrium structure in which all three atoms of the water lie in the CCC plane of propane, with torsional tunneling about the hydrogen bond occurring on the same time scale as the overall rotation. The small internal rotation tunneling splittings that occur in the rotational spectrum of the propane monomer are not observed in the spectrum of the complex