Endometrial Histology of Depomedroxyprogesterone Acetate Users: A Pilot Study

Objective. To obtain pilot data on the endometrial histology of Depomedroxyprogesterone acetate (Depo-Provera, DMPA) users experiencing breakthrough bleeding (BTB) versus users with amenorrhea. To compare the endometrial histology of patients who used DMPA continuously for 3–12 months versus those who used it for 13 months or more. Methods. Cross-sectional study. Endometrial biopsy was obtained fromall consenting patients who used DMPA for at least 3 months. Patients were divided into those with BTB in the last 3 months versus those with amenorrhea for at least 3 months. Histology results and duration of therapy were compared. Results. The proportion of women with chronic endometritis, uterine polyps, atrophic, proliferative, or progesteronedominant endometrium did not differ between those DMPA users with BTB versus those with amenorrhea. Duration of therapy did not correlate with symptoms of BTB or endometrial histology. Chronic endometritis was the most common histologic finding (10/40, 25%) and occurred more often in women experiencing BTB (35% versus 15%) (RR 1.62 CI 0.91–2.87). Moreover, 45% of women with BTB had received DMPA for more than 12 months. Conclusions. BTB was more common than previously reported in women using DMPA for more than 12 months. Chronic endometritis, which may indicate an underlying infectious or intracavitary anatomic etiology, has not been previously reported as a frequent finding in DMPA users, and may be related to ethnic or other sociodemographic characteristics of our patient population. Further study to elucidate the etiology of chronic endometritis in these patients is warranted

Locally Checkable Problems Parameterized by Clique-Width

We continue the study initiated by Bonomo-Braberman and Gonzalez in 2020 on r-locally checkable problems. We propose a dynamic programming algorithm that takes as input a graph with an associated clique-width expression and solves a 1-locally checkable problem under certain restrictions. We show that it runs in polynomial time in graphs of bounded clique-width, when the number of colors of the locally checkable problem is fixed. Furthermore, we present a first extension of our framework to global properties by taking into account the sizes of the color classes, and consequently enlarge the set of problems solvable in polynomial time with our approach in graphs of bounded clique-width. As examples, we apply this setting to show that, when parameterized by clique-width, the [k]-Roman domination problem is FPT, and the k-community problem, Max PDS and other variants are XP

Mechanical and hydraulic properties of the excavation damaged zone (EDZ) in the Opalinus Clay of the Mont Terri rock laboratory, Switzerland

Construction of cavities in the subsurface is always accompanied by excavation damage. Especially in the context of deep geological nuclear waste disposal, the evolving excavation damaged zone (EDZ) in the near field of emplacement tunnels is of utmost importance concerning safety aspects. As the EDZ differs from the intact host rock due to enhanced hydraulic transmissivity and altered geomechanical behavior, reasonable and location-dependent input data on hydraulic and mechanical properties are crucial. Thus, in this study, a hydromechanical characterization of an EDZ in the Mont Terri underground rock laboratory, Switzerland, was performed using three different handheld devices: (1) air permeameter, (2) microscopic camera and (3) needle penetrometer. The discrete fracture network (DFN), consisting of artificially induced unloading joints and reactivated natural discontinuities, was investigated by a portable air permeameter and combined microscopic imaging with automatic evaluation. Geomechanical and geophysical characterization of the claystone was conducted based on needle penetrometer testing at the exposed rock surface. Within the EDZ, permeable fractures with a mean hydraulic aperture of 84 ± 23 µm are present. Under open conditions, self-sealing of fractures is suppressed, and cyclic long-term fracture aperture oscillations in combination with closure resulting from convergence processes is observed. Based on measured needle penetration indices, a uniaxial compressive strength of 30 ± 13 MPa (normal to bedding) and 18 ± 8 MPa (parallel to bedding) was determined. Enhanced strength and stiffness are directly related to near-surface desaturation of the claystone and a sharp decrease in water content from 6.6 wt % to 3.7 wt %. The presented methodological approach is particularly suitable for time-dependent monitoring of EDZs since measurements are nondestructive and do not change the actual state of the rock mass. This allows for a spatially resolved investigation of hydraulic and mechanical fracture apertures, fracture surface roughness, and physico-mechanical rock parameters and their intra-facies variability

A new laser-ranged satellite for General Relativity and space geodesy: I. An introduction to the LARES2 space experiment

We introduce the LARES 2 space experiment recently approved by the Italian Space Agency (ASI). The LARES 2 satellite is planned for launch in 2019 with the new VEGA C launch vehicle of ASI, ESA and ELV. The orbital analysis of LARES 2 experiment will be carried out by our international science team of experts in General Relativity, theoretical physics, space geodesy and aerospace engineering. The main objectives of the LARES 2 experiment are gravitational and fundamental physics, including accurate measurements of General Relativity, in particular a test of frame-dragging aimed at achieving an accuracy of a few parts in a thousand, i.e., aimed at improving by about an order of magnitude the present state-of-the-art and forthcoming tests of this general relativistic phenomenon. LARES 2 will also achieve determinations in space geodesy. LARES 2 is an improved version of the LAGEOS 3 experiment, proposed in 1984 to measure frame-dragging and analyzed in 1989 by a joint ASI and NASA study

Alpha-conotoxin ImI disrupts central control of swimming in the medicinal leech

Medicinal leeches (Hirudo spp.) swim using a metachronal, front-to-back undulation. The behavior is generated by central pattern generators (CPGs) distributed along the animal’s midbody ganglia and is coordinated by both central and peripheral mechanisms. Here we report that a component of the venom of Conus imperialis, α-conotoxin ImI, known to block nicotinic acetyl-choline receptors in other species, disrupts swimming. Leeches injected with the toxin swam in circles with exaggerated dorsoventral bends and reduced forward velocity. Fictive swimming in isolated nerve cords was even more strongly disrupted, indicating that the toxin targets the CPGs and central coordination, while peripheral coordination partially rescues the behavior in intact animals

LAGEOS-type Satellites in Critical Supplementary Orbit Configuration and the Lense-Thirring Effect Detection

In this paper we analyze quantitatively the concept of LAGEOS--type satellites in critical supplementary orbit configuration (CSOC) which has proven capable of yielding various observables for many tests of General Relativity in the terrestrial gravitational field, with particular emphasis on the measurement of the Lense--Thirring effect.Comment: LaTex2e, 20 pages, 7 Tables, 6 Figures. Changes in Introduction, Conclusions, reference added, accepted for publication in Classical and Quantum Gravit

On Split B1-EPG Graphs

We consider the following problem: can a certain graph parameter of some given graph G be reduced by at least d, for some integer d, via at most k graph operations from some specified set S, for some given integer k? As graph parameters we take the chromatic number and the clique number. We let the set S consist of either an edge contraction or a vertex deletion. As all these problems are NP-complete for general graphs even if d is fixed, we restrict the input graph G to some special graph class. We continue a line of research that considers these problems for subclasses of perfect graphs, but our main results are full classifications, from a computational complexity point of view, for graph classes characterized by forbidding a single induced connected subgraph H

Rapid gene identification in sugar beet using deep sequencing of DNA from phenotypic pools selected from breeding panels

Ries D, Holtgräwe D, Viehöver P, Weisshaar B. Rapid gene identification in sugar beet using deep sequencing of DNA from phenotypic pools selected from breeding panels. BMC Genomics. 2016;17(1): 236.Background The combination of bulk segregant analysis (BSA) and next generation sequencing (NGS), also known as mapping by sequencing (MBS), has been shown to significantly accelerate the identification of causal mutations for species with a reference genome sequence. The usual approach is to cross homozygous parents that differ for the monogenic trait to address, to perform deep sequencing of DNA from F2 plants pooled according to their phenotype, and subsequently to analyze the allele frequency distribution based on a marker table for the parents studied. The method has been successfully applied for EMS induced mutations as well as natural variation. Here, we show that pooling genetically diverse breeding lines according to a contrasting phenotype also allows high resolution mapping of the causal gene in a crop species. The test case was the monogenic locus causing red vs. green hypocotyl color in Beta vulgaris (R locus). Results We determined the allele frequencies of polymorphic sequences using sequence data from two diverging phenotypic pools of 180 B. vulgaris accessions each. A single interval of about 31 kbp among the nine chromosomes was identified which indeed contained the causative mutation. Conclusions By applying a variation of the mapping by sequencing approach, we demonstrated that phenotype-based pooling of diverse accessions from breeding panels and subsequent direct determination of the allele frequency distribution can be successfully applied for gene identification in a crop species. Our approach made it possible to identify a small interval around the causative gene. Sequencing of parents or individual lines was not necessary. Whenever the appropriate plant material is available, the approach described saves time compared to the generation of an F2 population. In addition, we provide clues for planning similar experiments with regard to pool size and the sequencing depth required