42 research outputs found
Spatial Mapping of Powder Layer Density for Metal Additive Manufacturing via X-ray Microscopy
Uniform powder spreading is a requisite for creating consistent, high-quality
components via powder bed additive manufacturing (AM), wherein layer density
and uniformity are complex functions of powder characteristics, spreading
kinematics, and mechanical boundary conditions. High spatial variation in
particle packing density, driven by the stochastic nature of the spreading
process, impedes optical interrogation of these layer attributes. Thus, we
present transmission X-ray imaging as a method for directly mapping the
effective depth of powder layers at process-relevant scale and resolution.
Specifically, we study layers of nominal 50-250 micrometer thickness, created
by spreading a selection of commercially obtained Ti-6Al-4V, 316 SS, and
Al-10Si-Mg powders into precision-depth templates. We find that powder layer
packing fraction may be predicted from a combination of the relative thickness
of the layer as compared to mean particle size, and flowability assessed by
macroscale powder angle of repose. Power spectral density analysis is
introduced as a tool for quantification of defect severity as a function of
morphology, and enables separate consideration of layer uniformity and
sparsity. Finally, spreading is studied using multi-layer templates, providing
insight into how particles interact with both previously deposited material and
abrupt changes in boundary condition. Experimental results are additionally
compared to a purpose-built discrete element method (DEM) powder spreading
simulation framework, clarifying the competing role of adhesive and
gravitational forces in layer uniformity and density, as well as particle
motion within the powder bed during spreading
Functional Characterization of Rare RAB12 Variants and Their Role in Musician's and Other Dystonias
Mutations in RAB (member of the Ras superfamily) genes are increasingly recognized as cause of a variety of disorders including neurological conditions. While musician's dystonia (MD) and writer's dystonia (WD) are task-specific movement disorders, other dystonias persistently affect postures as in cervical dystonia. Little is known about the underlying etiology. Next-generation sequencing revealed a rare missense variant (c.586A>
G; p.Ile196Val) in RAB12 in two of three MD/WD families. Next, we tested 916 additional dystonia patients; 512 Parkinson's disease patients; and 461 healthy controls for RAB12 variants and identified 10 additional carriers of rare missense changes among dystonia patients (1.1%) but only one carrier in non-dystonic individuals (0.1%; p = 0.005). The detected variants among index patients comprised p.Ile196Val (n = 6); p.Ala174Thr (n = 3); p.Gly13Asp; p.Ala148Thr; and p.Arg181Gln in patients with MD; cervical dystonia; or WD. Two relatives of MD patients with WD also carried p.Ile196Val. The two variants identified in MD patients (p.Ile196Val; p.Gly13Asp) were characterized on endogenous levels in patient-derived fibroblasts and in two RAB12-overexpressing cell models. The ability to hydrolyze guanosine triphosphate (GTP), so called GTPase activity, was increased in mutants compared to wildtype. Furthermore, subcellular distribution of RAB12 in mutants was altered in fibroblasts. Soluble Transferrin receptor 1 levels were reduced in the blood of all three tested p.Ile196Val carriers. In conclusion, we demonstrate an enrichment of missense changes among dystonia patients. Functional characterization revealed altered enzyme activity and lysosomal distribution in mutants suggesting a contribution of RAB12 variants to MD and other dystonias
Material dependence of 2
Calculations of the material dependence of 2H(d,p)3H cross section and neutron-to-proton branching ratio of d+d reactions have been performed including a concept of the 0+ threshold single particle resonance. The resonance has been assumed to explain the enhanced electron screening effect observed in the d+d reaction for different metallic targets. Here, we have included interference effects between the flat and resonance part of the cross section, which allowed us to enlighten observed suppression of the neutron channel in some metals such as Sr and Li. Since the position of the resonance depends on the screening energy that strongly depends on the local electron density. The resonance width, observed for the d+d reactions in the very hygroscopic metals (Sr and Li) and therefore probably contaminated by oxides, should be much larger than for other metals. Thus, the interference term of the cross section depending on the total resonance width provides the material dependences
Mercury Vapor Pressure of Flue Gas Desulfurization Scrubber Suspensions: Effects of pH Level, Gypsum, and Iron
Calcium-based scrubbers designed to absorb HCl and SO<sub>2</sub> from flue gases can also remove oxidized mercury. Dissolved
mercury
halides may have an appreciable partial vapor pressure. Chemical reduction
of the dissolved mercury may increase the Hg emission, thereby limiting
the coremoval of mercury in the wet scrubbing process. In this paper
we evaluate the effects of the pH level, different gypsum qualities,
and iron in flue gas desulfurization (FGD) scrubber suspensions. The
impact of these parameters on mercury vapor pressure was studied under
controlled laboratory conditions in model scrubber suspensions. A
major influence is exerted by pH values above 7, considerably amplifying
the mercury concentration in the vapor phase above the FGD scrubber
suspension. Gypsum also increases the mercury re-emission. FeÂ(III)
decreases and FeÂ(II) increases the vapor pressure significantly. The
consequences of the findings for a reliable coremoval of mercury in
FGD scrubbers are discussed. It is shown that there is an increased
risk of poor mercury capture in lime-based FGD scrubbers in comparison
to limestone FGD scrubbers
Material dependence of
Calculations of the material dependence of 2H(d,p)3H cross section and neutron-to-proton branching ratio of d+d reactions have been performed including a concept of the 0+ threshold single particle resonance. The resonance has been assumed to explain the enhanced electron screening effect observed in the d+d reaction for different metallic targets. Here, we have included interference effects between the flat and resonance part of the cross section, which allowed us to enlighten observed suppression of the neutron channel in some metals such as Sr and Li. Since the position of the resonance depends on the screening energy that strongly depends on the local electron density. The resonance width, observed for the d+d reactions in the very hygroscopic metals (Sr and Li) and therefore probably contaminated by oxides, should be much larger than for other metals. Thus, the interference term of the cross section depending on the total resonance width provides the material dependences
Material dependence of 2H(d,p)3H cross section at the very low energies
Calculations of the material dependence of 2H(d,p)3H cross section and neutron-to-proton branching ratio of d+d reactions have been performed including a concept of the 0+ threshold single particle resonance. The resonance has been assumed to explain the enhanced electron screening effect observed in the d+d reaction for different metallic targets. Here, we have included interference effects between the flat and resonance part of the cross section, which allowed us to enlighten observed suppression of the neutron channel in some metals such as Sr and Li. Since the position of the resonance depends on the screening energy that strongly depends on the local electron density. The resonance width, observed for the d+d reactions in the very hygroscopic metals (Sr and Li) and therefore probably contaminated by oxides, should be much larger than for other metals. Thus, the interference term of the cross section depending on the total resonance width provides the material dependences