11 research outputs found
Cloning whole bacterial genomes in yeast
Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules. We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation
Iliac Anastomotic Stenting With a Biodegradable Poly-L-Lactide Stent: A Preliminary Study After 1 and 6 Weeks
CCR5 expression and duration of high risk sexual activity among HIV-seronegative men who have sex with men
Initiation of subduction zones: implications for arc evolution and ophiolite development
𝜆ₛ: computable semantics for differentiable programming with higher-order functions and datatypes
Enfermagem e genética: uma crítica feminista rumo ao trabalho em equipes transdisciplinares Nursing and genetics: a feminist critique moves us towards transdisciplinary teams
A informação genética e as tecnologias são cada vez mais importantes no cuidado em saúde, não somente nos países desenvolvidos, mas no mundo todo. Vários fatores globais prometem aumentar a futura demanda para pesquisa e serviços de saúde em genética moralmente conscientes. Embora os enfermeiros sejam o maior grupo profissional, prestando cuidado de saúde no mundo todo, eles não tomaram a liderança rumo a esse desafio. Insights, a partir da análise feminista, ajudam a esclarecer algumas das relações sociais e dos obstáculos culturais que têm impedido a integração da tecnologia genética na área da enfermagem. Um modelo alternativo é sugerido - o modelo transdisciplinar -, o qual foi desenvolvido inicialmente por uma enfermeira e introduzido, na década de 1970, na prestação do cuidado em saúde e no serviço social para crianças com déficit de desenvolvimento. Esse modelo holístico permite que todo profissional de saúde tenha a mesma voz ao determinar como o cuidado de saúde em genética será globalizado.<br>Genetic information and technologies are increasingly important in health care, not only in technologically advanced countries, but world-wide. Several global factors promise to increase future demand for morally conscious genetic health services and research. Although they are the largest professional group delivering health care world-wide, nurses have not taken the lead in meeting this challenge. Insights from feminist analysis help to illuminate some of the social institutions and cultural obstacles that have impeded the integration of genetics technology into the discipline of nursing. An alternative model is suggested - the transdisciplinary model - which was developed initially by a nurse and introduced in the 1970s into the delivery of health care and social services for children with developmental disabilities. This holistic model enables all health care professionals to have an equal voice in determining how genetic health care will be globalized