RÉGÉNÉRATION IN VITRO DE L’ARBRE À SUIF (PENTADESMA BUTYRACEA SABINE), UNE ESPÈCE LIGNEUSE À USAGES MULTIPLES (LUM) VULNÉRABLE AU BENIN

This work is done in order to identify the effects of basal media associated with two cytokinins (BAP and zeatin) and suitability for the regeneration of apical and axillary buds Pentadesma butyracea. Fragments axillary bud stem and apical bud stem fragment were taken from seedlings P. butyracea from seed germination in a greenhouse. After disinfection, explants were cultured on three basic areas: MS (Murashige and Skoog), B5 (Ganborg) and WPM (Woody Plant Medium). The effects of Zeatin and BAP on the bud and bud development were also studied. These in vitro propagation of tests conducted in this species, showed that the apical bud fragments proved to be the most favorable. Among the tested culture media, basal medium Gamborg (B5) is most suited to the entry into operation of apical buds (26.66%). Other WPM and MS basal media also allowed bud but at low rates (20%; 13%). Zeatin (3.5 mg / L) appears best suited to budding and regeneration of apical buds of P. butyracea and the formation of leaves per explants. BAP favored the resumption of activity of the apex, but not plant development. Our experiments allow to open a new path consistent multiplication of individuals elites Pentadesma butyracae to produce seeds that can be used for propagation, domestication and conservation of this species

Morphologie des fruits et quelques caractéristiques physique et chimique de l’huile et des tourteaux de Irvingia gabonensis (Irvingiaceae)

Irvingia gabonensis (Irvingiaceae) est un Produit Forestier Non Ligneux (PFNL) connu pour son fruit et son amande comestibles et dont les aspects ethnobotaniques sont largement discutés dans la littérature. Ce travail est une contribution à la caractérisation physique et chimique des fruits de l’espèce du sud-Bénin. Trois (03) formes physiques (cylindrique, sphérique et ellipsoïdale) sont notées da s les fruits collectés à Abomey-Calavi, Sè et Pobè avec une prépondérance des formes cylindriques. Les teneurs en matières grasses sont de l’ordre de 65% et les indices d’acide, d’iode, de peroxyde, de saponification et d’ester déterminés par lesnormes françaises révèlent la bonne qualité des échantillons d’huiles. L’analyse des esters méthyliques des acides gras de l’huile est faite par Chromatographie en Phase Gazeuse (CPG) et montre la prédominance des acides gras saturés (90%) dont les acides myristique C14:0 et laurique C12:0. L’analyse des tourteaux après extraction de l’huile montre une forte proportion de l’azote, du phosphore, du potassium, du magnésium et des protéines totales (20%). Ces tourteaux délipidés pourraient servir à l’assaisonnement des sauces au Bénin avecune bonne valeur nutritionnelle

RÉGÉNÉRATION IN VITRO DE L’ARBRE À SUIF (PENTADESMA BUTYRACEA SABINE), UNE ESPÈCE LIGNEUSE À USAGES MULTIPLES (LUM) VULNÉRABLE AU BENIN

This work is done in order to identify the effects of basal media associated with two cytokinins (BAP and zeatin) and suitability for the regeneration of apical and axillary buds Pentadesma butyracea. Fragments axillary bud stem and apical bud stem fragment were taken from seedlings P. butyracea from seed germination in a greenhouse. After disinfection, explants were cultured on three basic areas: MS (Murashige and Skoog), B5 (Ganborg) and WPM (Woody Plant Medium). The effects of Zeatin and BAP on the bud and bud development were also studied. These in vitro propagation of tests conducted in this species, showed that the apical bud fragments proved to be the most favorable. Among the tested culture media, basal medium Gamborg (B5) is most suited to the entry into operation of apical buds (26.66%). Other WPM and MS basal media also allowed bud but at low rates (20%; 13%). Zeatin (3.5 mg / L) appears best suited to budding and regeneration of apical buds of P. butyracea and the formation of leaves per explants. BAP favored the resumption of activity of the apex, but not plant development. Our experiments allow to open a new path consistent multiplication of individuals elites Pentadesma butyracae to produce seeds that can be used for propagation, domestication and conservation of this species

Exposure Patterns Driving Ebola Transmission in West Africa:A Retrospective Observational Study

BackgroundThe ongoing West African Ebola epidemic began in December 2013 in Guinea, probably from a single zoonotic introduction. As a result of ineffective initial control efforts, an Ebola outbreak of unprecedented scale emerged. As of 4 May 2015, it had resulted in more than 19,000 probable and confirmed Ebola cases, mainly in Guinea (3,529), Liberia (5,343), and Sierra Leone (10,746). Here, we present analyses of data collected during the outbreak identifying drivers of transmission and highlighting areas where control could be improved.Methods and findingsOver 19,000 confirmed and probable Ebola cases were reported in West Africa by 4 May 2015. Individuals with confirmed or probable Ebola ("cases") were asked if they had exposure to other potential Ebola cases ("potential source contacts") in a funeral or non-funeral context prior to becoming ill. We performed retrospective analyses of a case line-list, collated from national databases of case investigation forms that have been reported to WHO. These analyses were initially performed to assist WHO's response during the epidemic, and have been updated for publication. We analysed data from 3,529 cases in Guinea, 5,343 in Liberia, and 10,746 in Sierra Leone; exposures were reported by 33% of cases. The proportion of cases reporting a funeral exposure decreased over time. We found a positive correlation (r = 0.35, p ConclusionsAchieving elimination of Ebola is challenging, partly because of super-spreading. Safe funeral practices and fast hospitalisation contributed to the containment of this Ebola epidemic. Continued real-time data capture, reporting, and analysis are vital to track transmission patterns, inform resource deployment, and thus hasten and maintain elimination of the virus from the human population

Etude de la composition chimique de l'huile essentielle extraite des feuilles d'ocimum vasilicum L. du Benin

No Abstract

CARCINOMA SEBACE CUTANE

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Translocation (6;16) in a case of granulosa cell tumor of the ovary

We performed a cytogenetic study of an ovarian granulosa cell tumor (GCT). Tumor cells showed a translocation (6;16); the full karyotype was 45,XX- 6,dic(6;16)(q11;q22)/44,XX,-6,-22,dic(6;16)(q11;q22),-22/46,XX,- 6,dic(6;16)(q11;q22), +dic(6;16)(q11;q22). This is the second case of GCT with structural changes of chromosome 6 leading to loss of 6q material.SCOPUS: ar.jinfo:eu-repo/semantics/publishe