838 research outputs found
Integration over the quantum diagonal subgroup and associated Fourier-like algebras
By analogy with the classical construction due to Forrest, Samei and Spronk
we associate to every compact quantum group a completely
contractive Banach algebra , which can be viewed as a
deformed Fourier algebra of . To motivate the construction we first
analyse in detail the quantum version of the integration over the diagonal
subgroup, showing that although the quantum diagonal subgroups in fact never
exist, as noted earlier by Kasprzak and So{\l}tan, the corresponding
integration represented by a certain idempotent state on makes
sense as long as is of Kac type. Finally we analyse as an explicit
example the algebras , , associated to Wang's free
orthogonal groups, and show that they are not operator weakly amenable.Comment: Minor updates; Remark 5.7 has been added; 31 page
The Classic: Integration of Deoxyribonucleic Acid Specific for Rous Sarcoma Virus after Infection of Permissive and Nonpermissive Hosts: (RNA tumor viruses/reassociation kinetics/duck cells)
A relatively simple but stringent technique was developed to detect the integration of virus-specific DNA into the genomes of higher organisms. In both permissive (duck) and nonpermissive (mammalian) cells which normally contain no nucleotide sequences specific for Rous sarcoma virus, transformation by the virus results in the appearance of DNA specific for Rous sarcoma virus covalently integrated into strands of host-cell DNA containing reiterated sequences. Early after infection of mouse or duck cells by Rous sarcoma virus, unintegrated DNA specific for the virus can be demonstrated
Reconstitution of the quinoprotein methanol dehydrogenase from inactive Ca2+-free enzyme with Ca2+, Sr2+ or Ba2+
Shift invariant preduals of ℓ<sub>1</sub>(ℤ)
The Banach space ℓ<sub>1</sub>(ℤ) admits many non-isomorphic preduals, for
example, C(K) for any compact countable space K, along with many more
exotic Banach spaces. In this paper, we impose an extra condition: the predual
must make the bilateral shift on ℓ<sub>1</sub>(ℤ) weak<sup>*</sup>-continuous. This is
equivalent to making the natural convolution multiplication on ℓ<sub>1</sub>(ℤ)
separately weak*-continuous and so turning ℓ<sub>1</sub>(ℤ) into a dual Banach
algebra. We call such preduals <i>shift-invariant</i>. It is known that the
only shift-invariant predual arising from the standard duality between C<sub>0</sub>(K)
(for countable locally compact K) and ℓ<sub>1</sub>(ℤ) is c<sub>0</sub>(ℤ). We provide
an explicit construction of an uncountable family of distinct preduals which do
make the bilateral shift weak<sup>*</sup>-continuous. Using Szlenk index arguments, we
show that merely as Banach spaces, these are all isomorphic to c<sub>0</sub>. We then
build some theory to study such preduals, showing that they arise from certain
semigroup compactifications of ℤ. This allows us to produce a large number
of other examples, including non-isometric preduals, and preduals which are not
Banach space isomorphic to c<sub>0</sub>
Acidic residues in the membrane-proximal stalk region of vaccinia virus protein B5 are required for glycosaminoglycan-mediated disruption of the extracellular enveloped virus outer membrane
The extracellular enveloped virus (EEV) form of vaccinia virus (VACV) is surrounded by two lipid envelopes. This presents a topological problem for virus entry into cells, because a classical fusion event would only release a virion surrounded by a single envelope into the cell. Recently, we described a mechanism in which the EEV outer membrane is disrupted following interaction with glycosaminoglycans (GAGs) on the cell surface and thus allowing fusion of the inner membrane with the plasma membrane and penetration of a naked core into the cytosol. Here we show that both the B5 and A34 viral glycoproteins are required for this process. A34 is required to recruit B5 into the EEV membrane and B5 acts as a molecular switch to control EEV membrane rupture upon exposure to GAGs. Analysis of VACV strains expressing mutated B5 proteins demonstrated that the acidic stalk region between the transmembrane anchor sequence and the fourth short consensus repeat of B5 are critical for GAG-induced membrane rupture. Furthermore, the interaction between B5 and A34 can be disrupted by the addition of polyanions (GAGs) and polycations, but only the former induce membrane rupture. Based on these data we propose a revised model for EEV entry
Association of IL-4RA single nucleotide polymorphisms, HLA-DR and HLA-DQ in children with Alternaria-sensitive moderate-severe asthma
<p>Abstract</p> <p>Background</p> <p>Asthma afflicts 6% to 8% of the United States population, and severe asthma represents approximately 10% of asthmatic patients. Several epidemiologic studies in the United States and Europe have linked <it>Alternaria </it>sensitivity to both persistence and severity of asthma. In order to begin to understand genetic risk factors underlying <it>Alternaria </it>sensitivity and asthma, in these studies we examined T cell responses to <it>Alternaria </it>antigens, HLA Class II restriction and HLA-DQ protection in children with severe asthma.</p> <p>Methods</p> <p>Sixty children with <it>Alternaria</it>-sensitive moderate-severe asthma were compared to 49 children with <it>Alternaria</it>-sensitive mild asthma. We examined HLA-DR and HLA-DQ frequencies in <it>Alternaria</it>-sensitive asthmatic by HLA typing. To determine ratios of Th1/Th2 <it>Alternaria</it>-specific T-cells, cultures were stimulated in media alone, <it>Alternaria alternata </it>extract and Alt a1. Sensitivity to IL-4 stimulation was measured by up-regulation of CD23 on B cells.</p> <p>Results</p> <p>Children with <it>Alternaria</it>-sensitive moderate-severe asthma trended to have increased sensitivities to <it>Cladosporium </it>(46% versus 35%), to <it>Aspergillus </it>(43% versus 28%), and significantly increased sensitivities to trees (78% versus 57%) and to weeds (68% versus 48%). The IL-4RA ile75val polymorphism was significantly increased in <it>Alternaria</it>-sensitive moderate-severe asthmatics, 83% (0.627 allele frequency) compared to <it>Alternaria</it>-sensitive mild asthmatics, 57% (0.388 allele frequency). This was associated with increased sensitivity to IL-4 stimulation measured by significantly increased IL-4 stimulated CD23 expression on CD19+ and CD86+CD19+ B cells of <it>Alternaria</it>-sensitive moderate-severe asthmatics. IL-5 and IL-13 synthesis was significantly increased in <it>Alternaria</it>-sensitive moderate-severe asthmatics compared to mild asthmatics to <it>Alternaria </it>extract and Alt a1 stimulation. The frequency of HLA-DQB1*03 allele was significantly decreased in <it>Alternaria</it>-sensitive moderate-severe asthmatics compared to mild asthmatics, 39% versus 63%, with significantly decreased allele frequency, 0.220 versus 0.398.</p> <p>Summary</p> <p>In children with <it>Alternaria</it>-sensitive moderate severe asthma, there was an increased Th2 response to <it>Alternaria </it>stimulation and increased sensitivity to IL-4 stimulation. This skewing towards a Th2 response was associated with an increased frequency of the IL-4RA ile75val polymorphism. In evaluating the HLA association, there was a decreased frequency of HLA-DQB1*03 in <it>Alternaria</it>-sensitive moderate severe asthmatic children consistent with previous studies suggest that HLA-DQB1*03 may be protective against the development of mold-sensitive severe asthma.</p
Viroplasm and large virus-like particles in the dinoflagellate Gymnodinium uberrimum
Virus-like particles (VLPs) measuring 385±5 nm in diameter are described in the freshwater dinoflagellate Gymnodinium uberrimum . The VLPs are found in association with, and “budding” from a vesicular viroplasmic area. A similar viroplasm was also found in a chrysophycean alga, Mallomonas sp. collected from the same general area in Saginaw Bay of Lake Huron. The nature of these VLPs and their virogenic stroma, in these algae from the Laurentian Great Lakes are discussed in the present report.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41733/1/709_2005_Article_BF01275735.pd
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