Qualitative Behaviour Assessment of dogs in the shelter and home environment and relationship with quantitative behaviour assessment and physiological responses

Qualitative Behaviour Assessment (QBA) was utilised to examine the behavioural expression of dogs in different housing environments and the results were compared to measurements of quantitative behaviour and physiology. Firstly, quantitative behavioural and physiological differences were investigated between dogs in 3 housing environments (short-term shelter confinement, ≤ 4 days, n = 10; long-term shelter confinement, > 30 days, n = 9; and domestic living situations, n = 10). Each dog’s behaviour was recorded over a 4 h period using an ethogram consisting of 21 behaviour categories. Dogs in both short (SD) and long (LD) term confinement displayed higher frequencies of paw-lifting (P < 0.001), displacement behaviour (digging and/or drinking P < 0.01), vocalisation (P < 0.05) and locomotory activity (P < 0.001) compared to dogs maintained as family pets (PD). Salivary cortisol concentrations did not differ amongst groups (H = 0.55, P = 0.76). Secondly, quantitative behaviour and QBA were combined to investigate differences among these same 29 dogs when filmed for 1 min in both their Home Environment and a standardised Novel Environment. QBA of these video clips was made by 10 observers utilising Free-Choice-Profiling methodology. Generalised Procrustes Analysis was used to calculate a consensus profile and three main dimensions of dog expression in both Environments. The observers repeated dog scores on these dimensions with high accuracy (P < 0.001). Observers perceived dogs as more ‘relaxed/content’ in the Home Environment (H = 17.86, P < 0.0001), and more ‘calm/relaxed’ in the Novel Environment (H = 13.58, P < 0.001), than SD and LD dogs. In the Novel Environment, LD dogs were perceived as more ‘inquisitive/curious’ (H = 5.97, P < 0.05), and SD dogs as more ‘curious/cautious’ (H = 6.82, P < 0.05), than the other groups. Quantitative assessment of the 1 min Home and Novel Environment video clips were analysed using Principle Component Analysis (PCA), generating two main factors explaining 88% and 76% of the variation respectively. PCA factor 1 (‘rest’) and QBA Dimension 1 (‘relaxed/content’) correlated (P < 0.0001) in the Home Environment’. In the Novel Environment PCA factor 1 (‘stand’, ‘sniff’) correlated with QBA Dimension 1 (‘clam/relaxed’) and PCA factor 2 (‘sniff’, ‘walk’) correlated with QBA Dimension 2 (‘curious/inquisitive’). There was no correlation between QBA dimensions and cortisol concentrations. In sum, these results indicate that a combined quantitative/qualitative assessment facilitates the interpretation of behavioural variances resulting from housing differences and supports utilising QBA for the assessment of dog behavioural expression

Comparison of body mass index with waist circumference and skinfold-based percent body fat in firefighters: adiposity classification and associations with cardiovascular disease risk factors

PurposeThis study aims to examine whether body mass index (BMI) overestimates the prevalence of overweight or obese firefighters when compared to waist circumference (WC) and skinfold-based percent body fat (PBF) and to investigate differential relationships of the three adiposity measures with other biological cardiovascular disease (CVD) risk factors.MethodsThe adiposity of 355 (347 males and eight females) California firefighters was assessed using three different measures. Other CVD risk factors (high blood pressure, high lipid profiles, high glucose, and low VO2 max) of the firefighters were also clinically assessed.ResultsThe prevalence of total overweight and obesity was significantly (p < 0.01) higher by BMI (80.4 %) than by WC (48.7 %) and by PBF (55.6 %) in male firefighters. In particular, the prevalence of overweight firefighters was much higher (p < 0.01) by BMI (57.3 %) than by WC (24.5 %) and PBF (38.3 %). 60-64 % of male firefighters who were assessed as normal weight by WC and PBF were misclassified as overweight by BMI. When overweight by BMI was defined as 27.5-29.9 kg/m(2) (vs. the standard definition of 25.0-29.9 kg/m(2)), the agreement of the adiposity classification increased between BMI and other two adiposity measures. Obese firefighters had the highest CVD risk profiles across all three adiposity measures. Only when overweight by BMI was defined narrowly, overweight firefighters had substantially higher CVD risk profiles. Obesity and overweight were less prevalent in female and Asian male firefighters.ConclusionsBMI overestimated the prevalence of total overweight and obesity among male firefighters, compared to WC and skinfold-based PBF. Overweight by BMI needs to be more narrowly defined, or the prevalence of BMI-based overweight (27.5-29.9 kg/m(2)) should be reported additionally for prevention of CVD among male firefighters

Comparison of body mass index with waist circumference and skinfold-based percent body fat in firefighters: adiposity classification and associations with cardiovascular disease risk factors.

PurposeThis study aims to examine whether body mass index (BMI) overestimates the prevalence of overweight or obese firefighters when compared to waist circumference (WC) and skinfold-based percent body fat (PBF) and to investigate differential relationships of the three adiposity measures with other biological cardiovascular disease (CVD) risk factors.MethodsThe adiposity of 355 (347 males and eight females) California firefighters was assessed using three different measures. Other CVD risk factors (high blood pressure, high lipid profiles, high glucose, and low VO2 max) of the firefighters were also clinically assessed.ResultsThe prevalence of total overweight and obesity was significantly (p < 0.01) higher by BMI (80.4 %) than by WC (48.7 %) and by PBF (55.6 %) in male firefighters. In particular, the prevalence of overweight firefighters was much higher (p < 0.01) by BMI (57.3 %) than by WC (24.5 %) and PBF (38.3 %). 60-64 % of male firefighters who were assessed as normal weight by WC and PBF were misclassified as overweight by BMI. When overweight by BMI was defined as 27.5-29.9 kg/m(2) (vs. the standard definition of 25.0-29.9 kg/m(2)), the agreement of the adiposity classification increased between BMI and other two adiposity measures. Obese firefighters had the highest CVD risk profiles across all three adiposity measures. Only when overweight by BMI was defined narrowly, overweight firefighters had substantially higher CVD risk profiles. Obesity and overweight were less prevalent in female and Asian male firefighters.ConclusionsBMI overestimated the prevalence of total overweight and obesity among male firefighters, compared to WC and skinfold-based PBF. Overweight by BMI needs to be more narrowly defined, or the prevalence of BMI-based overweight (27.5-29.9 kg/m(2)) should be reported additionally for prevention of CVD among male firefighters

Evaluation of reticulated platelets in dogs with breed-related thrombocytopenia

The aim of this study was to evaluate the percentage of reticulated platelets in healthy dogs with breed-related thrombocytopenia. Seventy two dogs, clinically healthy, were enrolled in the study. Blood was collected from the patients and anticoagulated with tripotassium ethylenediaminetetraacetic acid (K3-EDTA) and sodium citrate. Platelet count was obtained by an impedance haematology analyser and platelet morphology was evaluated by examination of blood smears. Patients were allocated into two groups. Group 1 consisted of 30 dogs with normal platelet count, whereas group 2 was composed of 42 dogs with thrombocytopenia. Thrombocytopenia was present in both K3-EDTA and citrate blood samples. Patients with thrombocytopenia were divided into two subgroups: the first subgroup included dogs with platelet count in K3-EDTA anticoagulated blood from 100 to 200 x10⁹/L, patients in the second subgroup had a platelet count of less than 100 x10⁹/L. The percentage of young reticulated platelets (RPs) labelled with thiazole orange, and the percentage of platelets coated with platelet surface-associated IgG, were determined in platelet-rich plasma (PRP) by a flow cytometer. The mean percentage of RPs in K3-EDTA and citrate PRP was significantly higher in dogs with thrombocytopenia than in dogs with normal platelet count. The mean percentage of RPs was significantly higher in citrate PRP than in K3-EDTA PRP in all groups. The results suggest that idiopathic, asymptomatic thrombocytopenia is not caused by platelet surface-associated IgG. Dogs with breed-related thrombocytopenia have a competent bone marrow