Peirce Long Correspondence

Entries include typed letters, a biography written by Long\u27s relative, and a publisher advertisement with a photographic image of Long

Stellar Feedback and Resolved Stellar IFU Spectroscopy in the nearby Spiral Galaxy NGC 300

We present MUSE Integral Field Unit (IFU) observations of five individual HII regions in two giant (> 100 pc in radius) star-forming complexes in the low-metallicity (Z~0.33 Z) nearby (D ~ 2 Mpc) dwarf spiral galaxy NGC 300. We combine the IFU data with high spatial resolution HST photometry to demonstrate the extraction of stellar spectra and the classification of individual stars from ground-based data at the distance of 2 Mpc. For the two star-forming complexes, in which no O-type stars had previously been identified, we find a total of 13 newly identified O-type stars in the mass range 15-50 M, as well as 4 Wolf-Rayet stars. We use the derived massive stellar content to analyze the impact of stellar feedback on the HII regions. As already found for HII regions in the Magellanic Clouds, the dynamics of the analyzed NGC 300 HII regions are dominated by a combination of the pressure of the ionized gas and stellar winds. By comparing the derived ionized gas mass loading factors to the total gas mass loading factor across the NGC 300 disk, we find that the latter is an order of magnitude higher, either indicating very early evolutionary stages for these HII regions, or being a direct result of the multi-phase nature of feedback-driven bubbles. Moreover, we analyze the relation between the star formation rate and the pressure of the ionized gas as derived from small (<100 pc) scales, as both quantities are systematically overestimated when derived on galactic scales. With the wealth of ongoing and upcoming IFU instruments and programs, this study serves as a pathfinder for the systematic investigation of resolved stellar feedback in nearby galaxies, and it delivers the necessary analysis tools to enable massive stellar content and feedback studies sampling an unprecedented range of HII region properties across entire galaxies in the nearby Universe

Mary Clifford Webster Darling Correspondence

Entries include some correct biographical information typed by the Maine State Library and handwritten correspondence on a Fredericka Manor card and plain paper stationery

Potato Protein Isolate Stimulates Muscle Protein Synthesis at Rest and with Resistance Exercise in Young Women

Skeletal muscle myofibrillar protein synthesis (MPS) increases in response to protein feeding and to resistance exercise (RE), where each stimuli acts synergistically when combined. The efficacy of plant proteins such as potato protein (PP) isolate to stimulate MPS is unknown. We aimed to determine the effects of PP ingestion on daily MPS with and without RE in healthy women. In a single blind, parallel-group design, 24 young women (21 ± 3 years, n = 12/group) consumed a weight-maintaining baseline diet containing 0.8 g/kg/d of protein before being randomized to consume either 25 g of PP twice daily (1.6 g/kg/d total protein) or a control diet (CON) (0.8 g/kg/d total protein) for 2 wks. Unilateral RE (~30% of maximal strength to failure) was performed thrice weekly with the opposite limb serving as a non-exercised control (Rest). MPS was measured by deuterated water ingestion at baseline, following supplementation (Rest), and following supplementation + RE (Exercise). Ingestion of PP stimulated MPS by 0.14 ± 0.09 %/d at Rest, and by 0.32 ± 0.14 %/d in the Exercise limb. MPS was significantly elevated by 0.20 ± 0.11 %/d in the Exercise limb in CON (p = 0.008). Consuming PP to increase protein intake to levels twice the recommended dietary allowance for protein augmented rates of MPS. Performance of RE stimulated MPS regardless of protein intake. PP is a high-quality, plant-based protein supplement that augments MPS at rest and following RE in healthy young women

Effect of maternal panic disorder on mother-child interaction and relation to child anxiety and child self-efficacy

To determine whether mothers with panic disorder with or without agoraphobia interacted differently with their children than normal control mothers, 86 mothers and their adolescents (aged between 13 and 23 years) were observed during a structured play situation. Maternal as well as adolescent anxiety status was assessed according to a structured diagnostic interview. Results showed that mothers with panic disorder/agoraphobia showed more verbal control, were more criticizing and less sensitive during mother-child interaction than mothers without current mental disorders. Moreover, more conflicts were observed between mother and child dyadic interactions when the mother suffered from panic disorder. The comparison of parenting behaviors among anxious and non-anxious children did not reveal any significant differences. These findings support an association between parental over-control and rejection and maternal but not child anxiety and suggest that particularly mother anxiety status is an important determinant of parenting behavior. Finally, an association was found between children’s perceived self-efficacy, parental control and child anxiety symptoms

The role of the RACK1 ortholog Cpc2p in modulating pheromone-induced cell cycle arrest in fission yeast

The detection and amplification of extracellular signals requires the involvement of multiple protein components. In mammalian cells the receptor of activated C kinase (RACK1) is an important scaffolding protein for signal transduction networks. Further, it also performs a critical function in regulating the cell cycle by modulating the G1/S transition. Many eukaryotic cells express RACK1 orthologs, with one example being Cpc2p in the fission yeast Schizosaccharomyces pombe. In contrast to RACK1, Cpc2p has been described to positively regulate, at the ribosomal level, cells entry into M phase. In addition, Cpc2p controls the stress response pathways through an interaction with Msa2p, and sexual development by modulating Ran1p/Pat1p. Here we describe investigations into the role, which Cpc2p performs in controlling the G protein-mediated mating response pathway. Despite structural similarity to Gβ-like subunits, Cpc2p appears not to function at the G protein level. However, upon pheromone stimulation, cells overexpressing Cpc2p display substantial cell morphology defects, disorientation of septum formation and a significantly protracted G1 arrest. Cpc2p has the potential to function at multiple positions within the pheromone response pathway. We provide a mechanistic interpretation of this novel data by linking Cpc2p function, during the mating response, with its previous described interactions with Ran1p/Pat1p. We suggest that overexpressing Cpc2p prolongs the stimulated state of pheromone-induced cells by increasing ste11 gene expression. These data indicate that Cpc2p regulates the pheromone-induced cell cycle arrest in fission yeast by delaying cells entry into S phase

Interferon-λ rs12979860 genotype and liver fibrosis in viral and non-viral chronic liver disease

Tissue fibrosis is a core pathologic process that contributes to mortality in ∼45% of the population and is likely to be influenced by the host genetic architecture. Here we demonstrate, using liver disease as a model, that a single-nucleotide polymorphism (rs12979860) in the intronic region of interferon-λ4 (IFNL4) is a strong predictor of fibrosis in an aetiology-independent manner. In a cohort of 4,172 patients, including 3,129 with chronic hepatitis C (CHC), 555 with chronic hepatitis B (CHB) and 488 with non-alcoholic fatty liver disease (NAFLD), those with rs12979860CC have greater hepatic inflammation and fibrosis. In CHC, those with rs12979860CC also have greater stage-constant and stage-specific fibrosis progression rates (P<0.0001 for all). The impact of rs12979860 genotypes on fibrosis is maximal in young females, especially those with HCV genotype 3. These findings establish rs12979860 genotype as a strong aetiology-independent predictor of tissue inflammation and fibrosis

The impact of pre-supernova feedback and its dependence on environment

Integral field units enable resolved studies of a large number of star-forming regions across entire nearby galaxies, providing insight on the conversion of gas into stars and the feedback from the emerging stellar populations over unprecedented dynamic ranges in terms of spatial scale, star-forming region properties, and environments. We use the VLT/MUSE legacy data set covering the central $35$ arcmin$^{2}$ (${\sim}12$ kpc$^{2}$) of the nearby galaxy NGC 300 to quantify the effect of stellar feedback as a function of the local galactic environment. We extract spectra from emission line regions identified within dendrograms, combine emission line ratios and line widths to distinguish between HII regions, planetary nebulae, and supernova remnants, and compute their ionised gas properties, gas-phase oxygen abundances, and feedback-related pressure terms. For the HII regions, we find that the direct radiation pressure ($P_\mathrm{dir}$) and the pressure of the ionised gas ($P_{HII}$) weakly increase towards larger galactocentric radii, i.e. along the galaxy's (negative) abundance and (positive) extinction gradients. While the increase of $P_{HII}$ with galactocentric radius is likely due to higher photon fluxes from lower-metallicity stellar populations, we find that the increase of $P_\mathrm{dir}$ is likely driven by the combination of higher photon fluxes and enhanced dust content at larger galactocentric radii. In light of the above, we investigate the effect of increased pre-supernova feedback at larger galactocentric distances (lower metallicities and increased dust mass surface density) on the ISM, finding that supernovae at lower metallicities expand into lower-density environments, thereby enhancing the impact of supernova feedback