The acridonecarboxamide GF120918 potently reverses P-glycoprotein-mediated resistance in human sarcoma MES-Dx5 cells

The doxorubicin-selected, P-glycoprotein (P-gp)-expressing human sarcoma cell line MES-Dx5 showed the following levels of resistance relative to the non-P-gp-expressing parental MES-SA cells in a 72 h exposure to cytotoxic drugs: etoposide twofold, doxorubicin ninefold, vinblastine tenfold, taxotere 19-fold and taxol 94-fold. GF120918 potently reversed resistance completely for all drugs. The EC50s of GF120918 to reverse resistance of MES-Dx5 cells were: etoposide 7 ± 2 nM, vinblastine 19 ± 3 nM, doxorubicin 21 ± 6 nM, taxotere 57 ± 14 nM and taxol 91 ± 23 nM. MES-Dx5 cells exhibited an accumulation deficit relative to the parental MES-SA cells of 35% for [3H]-vinblastine, 20% for [3H]-taxol and [14C]-doxorubicin. The EC50 of GF120918, to reverse the accumulation deficit in MES-Dx5 cells, ranged from 37 to 64 nM for all three radiolabelled cytotoxics. [3H]-vinblastine bound saturably to membranes from MES-Dx5 cells with a KD of 7.8 ± 1.4 nM and a Bmax of 5.2 ± 1.6 pmol mg–1 protein. Binding of [3H]-vinblastine to P-gp in MES-Dx5 membranes was inhibited by GF120918 (Ki = 5 ± 1 nM), verapamil (Ki = 660 ± 350 nM) and doxorubicin (Ki = 6940 ± 2100 nM). Taxol, an allosteric inhibitor of [3H]-vinblastine binding to P-gp, could only displace 40% of [3H]-vinblastine (Ki = 400 ± 140 nM). The novel acridonecarboxamide derivative GF120918 potently overcomes P-gp-mediated multidrug resistance in the human sarcoma cell line MES-Dx5. Detailed analysis revealed that five times higher GF120918 concentrations were needed to reverse drug resistance to taxol in the cytotoxicity assay compared to doxorubicin, vinblastine and etoposide. An explanation for this phenomenon had not been found. © 1999 Cancer Research Campaig

Laser Pointer Tracking in Projector-Augmented Architectural Environments

We present a system that applies a custom-built pan-tilt-zoom camera for laser-pointer tracking in arbitrary real environments. Once placed in a building environment, it carries out a fully automatic self-registration, registrations of projectors, and sampling of surface parameters, such as geometry and reflectivity. After these steps, it can be used for tracking a laser spot on the surface as well as an LED marker in 3D space, using inter-playing fisheye context and controllable detail cameras. The captured surface information can be used for masking out areas that are critical to laser-pointer tracking, and for guiding geometric and radiometric image correction techniques that enable a projector-based augmentation on arbitrary surfaces. We describe a distributed software framework that couples laser-pointer tracking for interaction, projector-based AR as well as video see-through AR for visualizations with the domain specific functionality of existing desktop tools for architectural planning, simulation and building surveying

Photochemistry of HOx in the upper troposphere at northern midlatitudes

The factors controlling the concentrations of HOx radicals (= OH + peroxy) in the upper troposphere (8-12 km) are examined using concurrent aircraft observations of OH, HO2, H2O2, CH3OOH, and CH2O made during the Subsonic Assessment Ozone and Nitrogen Oxide Experiment (SONEX) at northern midlatitudes in the fall. These observations, complemented by concurrent measurements of O3, H2O, NO, peroxyacetyl nitrate (PAN), HNO3, CH4, CO, acetone, hydrocarbons, actinic fluxes, and aerosols, allow a highly constrained mass balance analysis of HOx and of the larger chemical family HOy (= HOx + 2 H2O2 + 2 CH3OOH + HNO2 + HNO4). Observations of OH and HO2 are successfully simulated to within 40% by a diel steady state model constrained with observed H2O2 and CH3OOH. The model captures 85% of the observed HOx variance, which is driven mainly by the concentrations of NOx (= NO + NO2) and by the strength of the HOx primary sources. Exceptions to the good agreement between modeled and observed HOx are at sunrise and sunset, where the model is too low by factors of 2-5, and inside cirrus clouds, where the model is too high by factors of 1.2-2. Heterogeneous conversion of NO2 to HONO on aerosols (γNO2=10-3) during the night followed by photolysis of HONO could explain part of the discrepancy at sunrise. Heterogeneous loss of HO2 on ice crystals (γice_HO2=0.025) could explain the discrepancy in cirrus. Primary sources of HOx from O(1D)+H2O and acetone photolysis were of comparable magnitude during SONEX. The dominant sinks of HOy were OH+HO2 (NOx<50 parts per trillion by volume (pptv)) and OH+HNO4 (NOx>50 pptv). Observed H2O2 concentrations are reproduced by model calculations to within 50% if one allows in the model for heterogeneous conversion of HO2 to H2O2 on aerosols (γHO2=0.2). Observed CH3OOH concentrations are underestimated by a factor of 2 on average. Observed CH2O concentrations were usually below the 50 pptv detection limit, consistent with model results; however, frequent occurrences of high values in the observations (up to 350 pptv) are not captured by the model. These high values are correlated with high CH3OH and with cirrus clouds. Heterogeneous oxidation of CH3OH to CH2O on aerosols or ice crystals might provide an explanation (γice_CH3OH∼0.01 would be needed). Copyright 2000 by the American Geophysical Union

Tindak Tutur Ilokusi Pada Mention Confess (Menfess) Di Akun Twitter Umsfess

The aims of this study are: (1) to describe the context of speech acts used by students in sending menfess on the UMSfess twitter social media account, (2) to describe the types of speech act functions used by students in sending menfess on the UMSfess twitter social media account, (3) to describe the purpose used by students in sending menfess on the UMSfess twitter social media account. The data in this study are menfess tweets from the UMSfess account. The data source of this research is the Menfess tweet sent by students from the UMSfess account. Data collection uses the technique of listening and taking notes. The data analysis method used the matching method and the agih method. The results of the research that have been found are in the first formulation found the context of inviting, the context of asking or asking, the context of offering, the context of telling, the context of complaining, the context of congratulating, and the context of thanking. The second formulation found that the illocutionary speech act function consisted of 6 data of illocutionary speech acts of assertive function, 9 data of illocutionary speech acts of directive function, 4 data of illocutionary speech acts of commissive function, 4 data of speech acts of expressive function, and 2 data of speech acts of declarative function. The third formulation was found, namely Competitive (the purpose of asking for prayer, the purpose of asking for help, and the purpose of asking), Fun (the purpose of inviting, the purpose of offering, and the purpose of congratulating), Cooperating (the purpose of stating), and Conflicting (the purpose of threatening, and complaining)

Analisis Pengaruh CAR, NPF, BOPO Dan FDR Terhadap Profitabilitas Bank Syariah Di Indonesia Tahun 2014-2017

This research uses to know and analyse the influence of CAR, NPF, BOPO and FDR toward the profitability of Syariah Bank in Indonesia. This research also uses to know the most dominant influence between CAR, NPF, BOPO and FDR toward profitability of Syariah Bank in Indonesia. The result of this research can be use as the review of the company in evaluating CAR, NPF, BOPO and FDR that have run in the appropriate target or do the continuance evaluation. The method in this research uses Descriptive Analysis, Classic Assumption, and Multiple Linear Regression that has purpose to examine the numerical data by using the analysis, it can be gotten the result correlation between independent and dependent variable. The population in this research is the entire Syariah Bank in Indonesia, in the other hand the sample of the research is the eleven Syariah Bank with purposive sampling technique as the technique of interpretation sample. Based on the result of the research to influence CAR, NPF, BOPO, and FDR toward the profiability (ROA), it can be gotten that the result of CAR has positive influence and significant toward ROA. BOPO has negative influence and significant toward ROA and FDR has negative and significant toward ROA

Pengembangan Media Pembelajaran Aplikasi “Akuntansi UKM” Berbasis Android sebagai Penyusunan Laporan Keuangan untuk Meningkatkan Hasil Belajar Siswa Kelas XI Akuntansi di SMK Muhammadiyah Delanggu

This study aims to develop accounting learning media using the Android-based "UKM Accounting" application in the preparation of Financial Statements. In addition, this research is also used to create an effective learning process and can improve student learning outcomes. This research is a research development using Quasi Experiment method. This research was conducted at Muhammadiyah Delanggu Vocational School with the test subjects is XI Accounting class students as the experimental class and the control class. The data analysis technique used in this study was the T-test. The results showed that: 1) Based on research from material experts and media experts stated that accounting learning media using the Android-based "UKM Accounting" application is feasible to be used for learning as a learning medium in the preparation of Financial Statements. 2) The average of pre-test and post-test scores in the control class only had a difference of 18.63 from the pre-test score of 39.55 to 58.18 in the post-test scores so that it showed no significant improvement. 3) In the experimental class the pre-test score was 45.55 and the post-test score increased by 91.82 so it shows that after the use of accounting learning media using the Android-based "UKM Accounting" application there was an increase in student learning outcomes

PENGARUH KEPEMIMPINAN DAN KOMPENSASI TERHADAP JOB INSECURITY PADA KARYAWAN PT. DIRGANTARA INDONESIA KOTA BANDUNG

ABSTRACT This research aimed to determine the effect of Leadership and Compensation on Job Insecurity either partially or simultaneously at PT. Dirgantara Indonesia. The research method used is descriptive and verification research methods with a total sample of 101 respondents. Data collection techniques through field research conducted by observation, interviews and distribution of questionnaires and through library research. The analytical method used is multiple linear regression, partial and simultaneous correlation, partial and simultaneous determination coefficients and hypothesis testing. The results showed that the effect of Leadership and Compensation on Job Insecurity was simultaneously 53.6% and the remaining 46.4% affected several other variables not examined. Partially, leadership has an influence of 21% and compensation has an effect of 32.5%. Partial and simultaneous test hypotheses have a positive and significant effect between Leadership and Compensation on Job Insecurity. Keywords: Leadership, Compensation and Job Insecurit

Pengaruh Anonymous Reporting, Power Distance, Moral Intensity, Dan Professional Commitment Terhadap Whistleblowing Decisions (Studi Kasus pada Mahasiswa Akuntansi di Surakarta)

This study examines the effect of anonymous reporting, power distance, moral intensity, and professional commitment on the whistleblowing decisions of undergraduate accounting students in Surakarta. The difference with previous research is using accounting students from Surakarta as a population. The choice of Surakarta accounting students as research objects was due to the absence of previous research using research variables. The method used was a survey through a questionnaire. We used purposive sampling on 60 accounting students from PTN/PTS in Surakarta. The results showed that anonymous reporting, power distance, moral intensity, and professional commitment simultaneously affect accounting students. Power distance has a negative and significant effect, while anonymous reporting, professional commitment and moral intensity have a positive and significant effect on whistleblowin

Marketing Mix Politik dan Keputusan Memilih Kepala Daerah melalui Persepsi Nilai dan Positioning sebagai Variabel Intervening (Studi Pada Pemilihan Gubernur dan Wakil Gubernur Banten 2017).

Penelitian ini bertujuan untuk menguji dan membuktikan secara empiris pengaruh marketing mix politik terhadap keputusan memilih; marketing mix politik terhadap persepsi nilai; marketing mix politik terhadap positioning; persepsi nilai terhadap keputusan memilih; persepsi nilai terhadap positioning; positioning terhadap keputusan memilih; marketing mix politik terhadap keputusan memilih melalui persepsi nilai; marketing mix politik terhadap keputusan memilih melalui positioning; marketing mix politik terhadap keputusan memilih melalui persepsi nilai dan positioning. Secara teoritis hasil penelitian ini diharapkan dapat memperkaya dan melengkapi khasanah ilmu pengetahuan di bidang manajemen terutama teori political marketing, sehingga dapat bermanfaat bagi para akademisi dan praktisi. Penelitian berlokasi di Provinsi Banten dengan populasi penduduk Provinsi Banten yang memiliki hak suara dan tercatat pada Daftar Pemilih Tetap (DPT) tahun 2017 sebanyak 7.734.485 orang dengan sampel berjumlah 664 orang. Pengambilan sampel menggunakan metode probability sampling dengan teknik cluster sampling. Pengumpulan data diperoleh melalui kuesioner. Penelitian ini menggunakan pendekatan kuantitatif dengan alat analisis SEM (Structural Equation Modeling), dan dengan bantuan program WarpPLS 6.0. Hasil penelitian menunjukkan bahwa: (1) marketing mix politik berpengaruh positif terhadap keputusan memilih, (2) marketing mix politik berpengaruh positif terhadap persepsi nilai, (3) marketing mix politik berpengaruh positif terhadap positioning, (4) persepsi nilai berpengaruh positif terhadap keputusan memilih, (5) persepsi nilai berpengaruh positif terhadap positioning, (6) positioning berpengaruh positif terhadap keputusan memilih, (7) marketing mix politik berpengaruh positif terhadap keputusan memilih melalui persepsi nilai, (8) marketing mix politik berpengaruh positif terhadap keputusan memilih melalui positioning, (9) marketing mix politik berpengaruh positif terhadap keputusan memilih melalui persepsi nilai dan positionin

Evolution of High Trophic Diversity Based on Limited Functional Disparity in the Feeding Apparatus of Marine Angelfishes (f. Pomacanthidae)

The use of biting to obtain food items attached to the substratum is an ecologically widespread and important mode of feeding among aquatic vertebrates, which rarely has been studied. We did the first evolutionary analyses of morphology and motion kinematics of the feeding apparatus in Indo-Pacific members of an iconic family of biters, the marine angelfishes (f. Pomacanthidae). We found clear interspecific differences in gut morphology that clearly reflected a wide range of trophic niches. In contrast, feeding apparatus morphology appeared to be conserved. A few unusual structural innovations enabled angelfishes to protrude their jaws, close them in the protruded state, and tear food items from the substratum at a high velocity. Only one clade, the speciose pygmy angelfishes, showed functional departure from the generalized and clade-defining grab-and-tearing feeding pattern. By comparing the feeding kinematics of angelfishes with wrasses and parrotfishes (f. Labridae) we showed that grab-and-tearing is based on low kinematics disparity. Regardless of its restricted disparity, the grab-and-tearing feeding apparatus has enabled angelfishes to negotiate ecological thresholds: Given their widely different body sizes, angelfishes can access many structurally complex benthic surfaces that other biters likely are unable to exploit. From these surfaces, angelfishes can dislodge sturdy food items from their tough attachments. Angelfishes thus provide an intriguing example of a successful group that appears to have evolved considerable trophic diversity based on an unusual yet conserved feeding apparatus configuration that is characterized by limited functional disparity