Somalia and the Pirates. ESF Working Paper No. 33, 18 December 2009

Piracy is defined by The Hague Centre for Strategic Studies as an "act of boarding or attempting to board any ship with the apparent intent to commit theft or any other crime and with the apparent intent or capability to use force in furtherance of that act." And it is estimated that from 1995 to 2009, around 730 persons were killed or are presumed dead, approximately 3,850 seafarers were held hostage, around 230 were kidnapped and ransomed, nearly 800 were seriously injured and hundreds more were threatened with guns and knives. (See paper by Rob de Wijk). In November 2009, CEPS held a European Security Forum seminar, in collaboration with the Institute for Strategic Studies, the Centre for the Democratic Control of Armed Forces and the Geneva Centre for Security Policy, to focus on the issue of Somalia and the Pirates, chaired by Francois Heisbourg. Four eminent specialists in this field: David Anderson, Rob de Wijk, Steven Haines and Jonathon Stevenson looked at the links with Somalia, and the historical, legal, political and security dimensions of the troubling success of piracy in today’s world. Their conclusions and recommendations for future action are brought together in this ESF 33 Working Paper

Seeking the right balance: NATO and EU in Dutch Foreign and Defense Policy

No passado, a política externa holandesa foi caracterizada pela expressão “paz, ganhos e princípios”. Na verdade, a política externa holandesa tem características semelhantes à de outras potências médias com desenvolvimento elevado. Enquanto instrumento de política externa, o uso ofensivo das suas forças armadas não é a primeira escolha; muito pelo contrário, o seu cerne está no reforço do primado do Direito Internacional e nas instituições, com o objectivo de criar relações internacionais estáveis e pacíficas. O que realmente distingue a Holanda é a sua ênfase nas relações transatlânticas como forma de manter a sua independência relativamente aos restantes poderes continentais. Devido às mudanças geoestratégicas dos anos 90, esta ênfase tem vindo a perder relevância. Dado que a Europa deixou de ser a prioridade estratégica dos EUA em matéria de segurança e defesa, a Holanda tenta encontrar um novo equilíbrio entre o tradicional transatlantismo, através da NATO, e a emergente Política Europeia de Segurança e Defesa (PESD) da União Europeia

Independence of Odor Quality and Absolute Sensitivity in a Study of Aging

Young, middle-aged, and senior subjects performed tasks designed to examine whether odor quality discrimination varies independently of sensitivity. One task entailed detection of 2-heptanone and the others AB-X discrimination of quality for sets of 2-heptanone and homologues or 2-heptanone and non-ketones. Subjects sought to discriminate either at intensity-matched concentrations far above threshold, but fixed across subjects, or at levels adjusted to neutralize differences in sensitivity. The young and middle-aged groups manifested the same absolute sensitivity, but the senior group poorer sensitivity. Performance in quality discrimination, however, declined progressively. Performance lacked an association with absolute sensitivity, no matter how examined. These data, in conjunction with converging findings from patients with neurological damage, studies of brain imaging, and the relation between concentration and quality discrimination in younger persons, suggest largely independent processing of odor quality and intensity

Dynamic texture perception in plant-based yogurt alternatives: Identifying temporal drivers of liking by TDS

As texture properties in novel food categories have a crucial role in consumer acceptance, mouthfeel profile of different plant-based yogurt-like semi-solid products were studied and compared to dairy yogurts. Mouthfeel properties of five plant-based yogurt-like products and two dairy yogurts were analyzed using temporal dominance of sensations (TDS) with consumers (n = 87). The attributes evaluated were thick, thin, creamy, watery, sticky, and foamy. Following TDS, overall liking and mouthfeel liking were evaluated using a 7-point hedonic scale. Temporal drivers of mouthfeel liking were studied using correspondence analysis and penalty-lift analysis with different time points during mastication. For penalty-lift analysis TDS data was analyzed as check-all-that apply (CATA) data. Results from the present work show that mouthfeel perception in non-dairy yogurt alternatives is a dynamic process. Attributes typically used to describe dairy yogurts are also relevant for describing non-dairy yogurt alternatives. Yogurt alternatives and dairy yogurts can be similar and equally liked by their mouthfeel profile. Temporal drivers of liking in plant-based products are thickness and creaminess and temporal drivers of disliking are thinness and wateriness. In this study, the first dominant attributes were found to have a stronger impact on mouthfeel liking than the dominant attributes perceived later during mastication.Peer reviewe

Fertigatie en plantdichtheden in prei

Zowel milieueisen als de noodzaak om constante kwaliteit en constant volume te produceren tegen een acceptabele kostprijs, zullen tot de ontwikkeling van nieuwe teeltsystemen leiden. Door ‘gestuurd’ te telen kan de bodem voornamelijk als substraat gebruikt gaan worden. Vooral in Zuidoost Nederland is de milieuproblematiek rond emissie van mineralen groot en prei is om dat gebied een belangrijke vollegrondsgroentegewas. Het brede doel is het ontwikkelen van innovatieve, gestuurde teeltsystemen voor prei die nieuwe perspectieven bieden aan de sector op gebied van markt (kwaliteit, kostprijs, constant volume), arbeid (arbeidsomstandigheden, arbeidsbehoefte) en regelgeving (milieueisen)

Dynamic control of selectivity in the ubiquitination pathway revealed by an ASP to GLU substitution in an intra-molecular salt-bridge network

Ubiquitination relies on a subtle balance between selectivity and promiscuity achieved through specific interactions between ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s). Here, we report how a single aspartic to glutamic acid substitution acts as a dynamic switch to tip the selectivity balance of human E2s for interaction toward E3 RING-finger domains. By combining molecular dynamic simulations, experimental yeast-two-hybrid screen of E2-E3 (RING) interactions and mutagenesis, we reveal how the dynamics of an internal salt-bridge network at the rim of the E2-E3 interaction surface controls the balance between an “open”, binding competent, and a “closed”, binding incompetent state. The molecular dynamic simulations shed light on the fine mechanism of this molecular switch and allowed us to identify its components, namely an aspartate/glutamate pair, a lysine acting as the central switch and a remote aspartate. Perturbations of single residues in this network, both inside and outside the interaction surface, are sufficient to switch the global E2 interaction selectivity as demonstrated experimentally. Taken together, our results indicate a new mechanism to control E2-E3 interaction selectivity at an atomic level, highlighting how minimal changes in amino acid side-chain affecting the dynamics of intramolecular salt-bridges can be crucial for protein-protein interactions. These findings indicate that the widely accepted sequence-structure-function paradigm should be extended to sequence-structure-dynamics-function relationship and open new possibilities for control and fine-tuning of protein interaction selectivity

Predatory mite attraction to herbivore-induced plant odors is not a consequence of attraction to individual herbivore-induced plant volatiles

Predatory mites locate herbivorous mites, their prey, by the aid of herbivore-induced plant volatiles (HIPV). These HIPV differ with plant and/or herbivore species, and it is not well understood how predators cope with this variation. We hypothesized that predators are attracted to specific compounds in HIPV, and that they can identify these compounds in odor mixtures not previously experienced. To test this, we assessed the olfactory response of Phytoseiulus persimilis, a predatory mite that preys on the highly polyphagous herbivore Tetranychus urticae. The responses of the predatory mite to a dilution series of each of 30 structurally different compounds were tested. They mites responded to most of these compounds, but usually in an aversive way. Individual HIPV were no more attractive (or less repellent) than out-group compounds, i.e., volatiles not induced in plants fed upon by spider-mites. Only three samples were significantly attractive to the mites: octan-1-ol, not involved in indirect defense, and cis-3-hexen-1-ol and methyl salicylate, which are both induced by herbivory, but not specific for the herbivore that infests the plant. Attraction to individual compounds was low compared to the full HIPV blend from Lima bean. These results indicate that individual HIPV have no a priori meaning to the mites. Hence, there is no reason why they could profit from an ability to identify individual compounds in odor mixtures. Subsequent experiments confirmed that naive predatory mites do not prefer tomato HIPV, which included the attractive compound methyl salicylate, over the odor of an uninfested bean. However, upon associating each of these odors with food over a period of 15 min, both are preferred. The memory to this association wanes within 24 hr. We conclude that P. persimilis possesses a limited ability to identify individual spider mite-induced plant volatiles in odor mixtures. We suggest that predatory mites instead learn to respond to prey-associated mixtures of volatiles and, thus, to odor blends as a whole