Challenges to implementing the food-based dietary guidelines in the South African primary school curriculum: a qualitative study exploring the perceptions of principals and curriculum advisors

Objectives: The South African food-based dietary guidelines (FBDG) were developed and implemented to promote healthy lifestyles in the population along with preventing non-communicable diseases and other forms of diet-related illness. The FBDG were recommended for implementation within the national school curriculum. The objective of this study was to explore perceived challenges to successful implementation of these guidelines in the primary school curriculum.Design: This qualitative study gathered data via semi-structured individual interviews. Data were transcribed and analysed with ATLAS.ti software, using a thematic approach.Setting: Public primary schools located in three education districts in the Western Cape, South Africa.Subjects: Principals at twelve schools from communities with different socioeconomic statuses, and five curriculum advisors of relevant subjects from provincial and district levels.Outcomes measures: Perceived barriers to implementing FBDG in the primary school curriculum.Results: A lack of knowledge and/or expertise related to the FBDG by educators, time constraints within the teaching programme and lifestyle related factors of educators, parents and learners emerged as the three main challenge themes from the interviews. A lack of resources was identified as an additional theme.Conclusions: The principals and curriculum advisors anticipated many barriers to the success of implementing nutrition education using the FBDG in the primary school curriculum. Adequately addressing such barriers through improved awareness and knowledge of the FBDG by school staff could improve the potential of using the FBDG as a nutrition education tool in primary schools in South Africa.Keywords: challenges, food-based dietary guidelines, implementation, school curriculum, South Afric

Ethical challenges in developing an educational video to empower potential participants during consent processes in future HIV cure research in South Africa

Obtaining consent for HIV research is complex, particularly in low- and middle-income countries. Low levels of education, complexity of science and research processes, confusion about basic elements of research, and socio-economic conditions that make access to medical care difficult have collectively led to concerns about the adequacy of the consent process. Given the exponential growth of HIV prevention and treatment research in South Africa, HIV researchers are increasingly facing challenges obtaining authentic informed consent from potential participants. It is anticipated that HIV cure research, despite being in its infancy in South Africa, will introduce a new discourse into a population that is often struggling to understand the differences between ‘cure’, ‘preventive and therapeutic vaccines’ and other elements of the research process. Coupled with this, South Africa has a complex history of ‘illegitimate’ or ‘false cures’ for HIV. It is therefore logical to anticipate that HIV cure research may face significant challenges during consent processes. HIV prevention research in South Africa has demonstrated the importance of early community engagement in educating potential research participants and promoting community acceptance of research. Consequently, in an attempt to extrapolate from this experience of engaging with communities early regarding cure research, a 15-minute educational video entitled ‘I have a dream: a world without HIV’ was developed to educate and ultimately empower potential research participants to make informed choices during consent processes in future HIV cure clinical trials. To aid others in the development of educational interventions, this paper discusses the challenges faced in developing this educational video

Arctic Sea Ice Volume and Mass from Data Fusion of CryoSat-2 and SMOS

The quantification of the sea ice mass balance as the marine part of the cryosphere by satellite observations depend on sea ice thickness data records for the entire ice-covered oceans. The challenges to this task are numerous. Sea ice itself is a highly dynamic medium with a significant variability at meter scale and a strong seasonal cycle which significantly impacts it remote sensing signature. Satellite sensors must therefore provide precise observations at high spatial resolution to observe the full spread of the sea ice thickness distribution and its governing processes such as the dynamic deformation. Average thickness values for larger areas are sufficient for mass balance estimates, however, available methods such as satellite altimetry and passive microwave remote sensing rely on indirect methods and auxiliary information and are often not able to provide information with an acceptable uncertainty for certain or thickness categories or during the presence of surface melt. In addition, suitable satellite sensors in orbits that enabling sea ice thickness retrieval in the inner Arctic Ocean have been in service only until recently in comparison to satellites capable of observing sea ice area. Thus, the assessment of the sea ice mass balance for longer time series is often based on reanalysis models and not Earth Observation data. The sea ice community also traditionally expresses the total sea ice budget volume and not mass. We will therefore present an available sea ice volume data record that is derived by data fusion of CryoSat-2 radar altimeter and SMOS L-Band passive microwave-based sea ice thickness information. Both methods have a complementary sensitivity to different thickness classes and optimal interpolation is employed for gap-less sea ice thickness information in the northern hemisphere since November 2010. The data record is generated for the ESA funded MOS & CryoSat-2 Sea Ice Data Product Processing and Dissemination Service (CS2SMOS-PDS). We discuss the characteristics of the data set and provide an overview of intended evolutions of the data set, specifically improvements to the spatial resolutions, a potential extension to the southern hemisphere and the addition of other available satellite sensors to the optimal interpolation. Within the context of the mass balance of the cryosphere we will share our thoughts on the significance of the CryoSat-2/SMOS based sea ice volume time series for climate applications in the context of its comparable short temporal and how this information can be presented more consistently to other components of the cryosphere

Intrinsic and Synthetic Stable Isotope Marking of Tsetse Flies

The sterile insect technique has been successfully used to eliminate tsetse populations in a number of programs. Program monitoring in the field relies on the ability to accurately differentiate released sterile insects from wild insects so that estimates can be made of the ratio of sterile males to wild males. Typically, released flies are marked with a dye, which is not always reliable. The difference in isotopic signatures between wild and factory-reared populations could be a reliable and intrinsic secondary marker to complement existing marking methods. Isotopic signatures are natural differences in stable isotope composition of organisms due to discrimination against the heavier isotopes during some biological processes. As the isotopic signature of an organism is mainly dependent on what it eats; by feeding factory-reared flies isotopically different diets to those of the wild population it is possible to intrinsically mark the flies. To test this approach unlabeled samples of Glossina pallidipes (Austen) (Diptera: Glossinidae) from a mass rearing facility and wild populations were analyzed to determine whether there were any natural differences in signatures that could be used as markers. In addition experiments were conducted in which the blood diet was supplemented with isotopically enriched compounds and the persistence of the marker in the offspring determined. There were distinct natural isotopic differences between factory reared and wild tsetse populations that could be reliably used as population markers. It was also possible to rear artificially isotopically labeled flies using simple technology and these flies were clearly distinguishable from wild populations with greater than 95% certainty after 85 days of “release”. These techniques could be readily adopted for use in SIT programs as complimentary marking techniques

Inhibition of native hepatitis C virus replicase by nucleotide and non-nucleoside inhibitors

AbstractA number of nucleotide and non-nucleoside inhibitors of HCV polymerase are currently under investigation as potential antiviral agents to treat HCV-infected patients. HCV polymerase is part of a replicase complex including the polymerase subunit NS5B together with other viral and host proteins and viral RNA. The RNA synthesis activity of the native replicase complex was inhibited by 3′-deoxy-CTP, a chain-terminating nucleotide analog, but not inhibited by non-nucleoside NS5B polymerase inhibitors of three different structural classes. The HCV replicase was also resistant to heparin, a broad-spectrum, RNA-competitive polymerase inhibitor. Prebinding of the recombinant NS5B protein with a RNA template rendered the polymerase largely resistant to the inhibition by heparin and the non-nucleoside inhibitors, but did not affect the inhibitory potency of 3′-deoxy-CTP. Therefore, the HCV replicase showed a similar pattern of inhibitor sensitivity as compared to RNA-bound NS5B. These results suggest that the native HCV replicase complex represents a stable and productive polymerase–RNA complex. The allosteric non-nucleoside NS5B polymerase inhibitors are inactive against established HCV replicase but may function antagonistically with the formation of a productive enzyme–template complex

A Sparse Bayesian Estimation Framework for Conditioning Prior Geologic Models to Nonlinear Flow Measurements

We present a Bayesian framework for reconstruction of subsurface hydraulic properties from nonlinear dynamic flow data by imposing sparsity on the distribution of the solution coefficients in a compression transform domain