77 research outputs found
Electron-impact Excitation Of The Lowest Four Excited States Of Argon
First-order many-body theory has been used to calculate the differential and integral cross sections for electron-impact excitation of the argon atom to the 4 P03, 4 P23, 4 P13, and 4 P11 electronic states, for incident electron energies of 16, 20, 30, 50, and 80.4 eV. The resulting cross sections are in good agreement with recent results extracted from electron energy-loss measurements on argon. Detailed calculations show that spin-orbit coupling must be included in the P13 wave function in order to describe the electron-impact excitation of the state properly. © 1981 The American Physical Society.2352194221
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Inertial fusion in the nineties
The 1980s has proven to be an exicting time for the inertial confinement fusion (ICF) program. Major new laser and light-ion drivers have been constructed and have produced some encouraging results. The 1990s will be a crucial time for the ICF program. A decision for proceeding with the next facility is scheduled for the early 1990s. If the decision is positive, planning and construction of this facility will occur. Depending on the time required for design and construction, this next-generation facility could become operational near the turn of the century. 21 refs., 4 figs
Directed evolution of biomass intensive CHO cells by adaptation to sub-physiological temperature
We report a simple and effective means to increase the biosynthetic capacity of host CHO cells. Lonza proprietary CHOK1SV® cells were evolved by serial sub-culture for over 150 generations at 32 °C. During this period the specific proliferation rate of hypothermic cells gradually recovered to become comparable to that of cells routinely maintained at 37 °C. Cold-adapted cell populations exhibited (1) a significantly increased volume and biomass content (exemplified by total RNA and protein), (2) increased mitochondrial function, (3) an increased antioxidant capacity, (4) altered central metabolism, (5) increased transient and stable productivity of a model IgG4 monoclonal antibody and Fc-fusion protein, and (6) unaffected recombinant protein N-glycan processing. This phenotypic transformation was associated with significant genome-scale changes in both karyotype and the relative abundance of thousands of cellular mRNAs across numerous functional groups. Taken together, these observations provide evidence of coordinated cellular adaptations to sub-physiological temperature. These data reveal the extreme genomic/functional plasticity of CHO cells, and that directed evolution is a viable genome-scale cell engineering strategy that can be exploited to create host cells with an increased cellular capacity for recombinant protein production
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ICF research at Los Alamos
It is apparent that short wavelength lasers (<500 nm) provide efficient coupling of laser energy into ICF target compression. KrF lasers (248 nm) operate at near-optimum wavelength and provide other potential benefits to ICF target coupling (e.g., bandwidth) and applications (high wallplug efficiency and relatively low cost). However, no driver technology has yet been shown to meet all of the requirements for a high-gain ICF capability at a currently acceptable cost, and there are still significant uncertainties in the driver-target coupling and capsule hydrodynamics that must be addressed. The Los Alamos research program is designed to assess the potential of KrF lasers for ICF and to determine the feasibility of achieving high gain in the laboratory with a KrF laser driver. Major efforts in KrF laser development and technology, target fabrication and materials development, and laser-matter interaction and hydrodynamics research are discussed. 27 refs., 10 figs
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