Amniotic fluid brain-specific proteins are biomarkers for spinal cord injury in experimental myelomeningocele

Myelomeningocele (MMC), the most severe form of spina bifida (SB), causes neurological deficit. Injury to the spinal cord is thought to begin in utero. We investigated whether brain-specific proteins (BSPs) would enable us to monitor the development of MMC-related tissue damage during pregnancy in an animal model with naturally occurring SB (curly tail/loop tail mouse; n = 256). Amniotic fluid levels of neurofilament heavy chain (NfH), glial acidic fibrillary protein (GFAP) and S100B were measured by standard ELISA techniques. The amniotic fluid levels of all BSPs were similar in SB and control mice on embryonic day (E) 12.5 and 14.5, whereas a significant increase was observed for GFAP in SB mice on E16.5. Levels of all BSPs were significantly increased in SB mice on E18.5. The rapid increase in GFAP, paralleled by a moderate increase in NfH and S100B, suggests that spinal cord damage starts to accelerate around E16.5. The macroscopic size of the MMC was related to NfH level on E16.5 and E18.5, suggesting that axonal degeneration is most severe in large MMC. Amniotic fluid BSP measurements may provide important information for balancing the risks and benefits to mother and child of in utero surgery for MMC

Building reflective practices in a pre-service math and science teacher education course that focuses on qualitative video analysis

The use of video for in-service and pre-service teacher development has been gaining acceptance, and yet video remains a challenging and understudied tool. Many projects have used video to help pre-service and in-service teachers reflect on their own teaching processes, examine teacher–student interactions, and develop their professional vision. But rarely has video been used in ways more akin to qualitative education research that is focused on student learning. Even more rarely has this focus occurred at the earliest stages of pre-service teaching when students have not yet decided to pursue teaching careers. Yet here we argue that there are benefits to our approach. We examine a course for prospective pre-service math and science teachers at the University of California, Berkeley, that engages participants in qualitative video analysis to foster their reflective practice. This course is unique in that the prospective pre-service teachers engage in qualitative video analysis at a level characteristic of professional educational research, in that their analysis focuses on student learning of math and science content. We describe classroom activities that provide opportunities for the preservice teacher participants to better observe, notice, and interpret their students’ sociocognitive activity. The course culmination project involves participants developing and teaching lessons in a high school classroom. The participants then videotape the lessons and conduct qualitative video analysis. Results include detailed examples of two selected prospective pre-service teachers demonstrating coherent and effective approaches to conceptualizing the learning and teaching of mathematical and science content along with some potential design principles for building reflective practices through qualitative video projects. © 2018 Association for Science Teacher Education

Discourse Analysis: varieties and methods

This paper presents and analyses six key approaches to discourse analysis, including political discourse theory, rhetorical political analysis, the discourse historical approach in critical discourse analysis, interpretive policy analysis, discursive psychology and Q methodology. It highlights differences and similarities between the approaches along three distinctive dimensions, namely, ontology, focus and purpose. Our analysis reveals the difficulty of arriving at a fundamental matrix of dimensions which would satisfactorily allow one to organize all approaches in a coherent theoretical framework. However, it does not preclude various theoretical articulations between the different approaches, provided one takes a problem-driven approach to social science as one?s starting-point

Effective algorithm of analysis of integrability via the Ziglin's method

In this paper we continue the description of the possibilities to use numerical simulations for mathematically rigorous computer assisted analysis of integrability of dynamical systems. We sketch some of the algebraic methods of studying the integrability and present a constructive algorithm issued from the Ziglin's approach. We provide some examples of successful applications of the constructed algorithm to physical systems.Comment: a figure added, version accepted to JDC

Real-time simulation of three-dimensional shoulder girdle and arm dynamics

Electrical stimulation is a promising technology for the restoration of arm function in paralyzed individuals. Control of the paralyzed arm under electrical stimulation, however, is a challenging problem that requires advanced controllers and command interfaces for the user. A real-time model describing the complex dynamics of the arm would allow user-in-the-loop type experiments where the command interface and controller could be assessed. Real-time models of the arm previously described have not included the ability to model the independently controlled scapula and clavicle, limiting their utility for clinical applications of this nature. The goal of this study therefore was to evaluate the performance and mechanical behavior of a real-time, dynamic model of the arm and shoulder girdle. The model comprises seven segments linked by eleven degrees of freedom and actuated by 138 muscle elements. Polynomials were generated to describe the muscle lines of action to reduce computation time, and an implicit, first-order Rosenbrock formulation of the equations of motion was used to increase simulation step-size. The model simulated flexion of the arm faster than real time, simulation time being 92% of actual movement time on standard desktop hardware. Modeled maximum isometric torque values agreed well with values from the literature, showing that the model simulates the moment-generating behavior of a real human arm. The speed of the model enables experiments where the user controls the virtual arm and receives visual feedback in real time. The ability to optimize potential solutions in simulation greatly reduces the burden on the user during development

Analysis of gait kinematics to determine the effect of manipulating the appearance of stairs to improve safety: a linked series of laboratory-based, repeated measures studies

Background: Falls on stairs are a common and dangerous problem for older people. This series of studies evaluated whether or not selected changes to the appearance of stairs could make them safer for older people to negotiate. Objectives: To determine the effect of (1) a step edge highlighter and its position and (2) an optimised horizontal–vertical (H–V) visual illusion placed on a step riser on gait safety during stair descent and ascent. Design: A series of studies using a repeated measures, laboratory-based design, investigating gait control and safety in independently mobile older people. Setting: The University of Bradford Vision and Mobility Laboratory. Participants: Fit and healthy older people aged 60 years of age or more, independently mobile, reasonably active and with normal healthy eyes and corrected vision. Interventions: A step edge highlighter in a variety of offsets from the stair edge and an optimised H–V visual illusion placed on the stair riser. The H–V illusion was provided on a staircase by horizontal step edge highlighters on the tread edges and vertical stripes on the step risers. Main outcome measures: Gait parameters that are important for safe stepping in ascent and descent, measured using three-dimensional lower limb segmental kinematic data

The effect of pre-exposure on family resemblance categorization for stimuli of varying levels of perceptual difficulty

This study investigated the effect that pre-exposure to a set of stimuli has on the prevalence of family resemblance categorization. 64 participants were tested to examine the effect that pre-exposure type (same-stimuli vs unrelated-stimuli) and the perceptual difficulty of the stimuli (perceptually similar vs perceptually different) has on categorization strategy. There was a significant effect of perceptual difficulty, indicating that perceptually different stimuli evoked a higher level of family resemblance sorting than perceptually similar stimuli. There was no significant main effect of pre-exposure type; however, there was a significant interaction between pre-exposure type and level of perceptual difficulty. Post-hoc tests revealed that this interaction was the result of an increase in family resemblance sorting for the perceptually different stimuli under relevant preexposure but no such effect for perceptually similar stimuli. The theoretical implications of these findings are discussed

What you see is what you step: the horizontal-vertical illusion increases toe clearance in older adults during stair ascent

PURPOSE Falls on stairs are a significant cause of morbidity and mortality in elderly people. A simple safety strategy to avoid tripping on stairs is increasing foot clearance. We determined whether a horizontal–vertical illusion superimposed onto stairs to create an illusory perceived increase in stair-riser height would increase stair ascent foot clearance in older participants

Case Report: Color as a Therapeutic Intervention

Peer reviewedFinal Accepted Versio