Advantage of use of activated flux-cored wire instead of solid wire with the mag welding process from the mechanical properties aspect

The objective of this paper is the analysis and evaluation of the quality of the new flux-cored wire designed for the MAG welding process that was developed and produced using special laboratory equipment. Experimental welding was performed with the new activated flux-cored wire and classic solid wire with changing of welding parameters and shielding gas composition (100 % CO2 and gas mixtures of Ar + CO2 + O2)

Bryophyte flora of the Uvac river gorge (Southwest Serbia)

In the examined area, 165 taxa were found and identified: 139 taxa from the class Bryopsida and 26 taxa from the class Marchantiopsida. Nine species are red-listed in Serbia. Material was collected from 62 localities, which were analyzed for similarity of chorological and ecological features using the Jaccard similarity index. Analysis of floristic elements and phytogeographic distribution showed that the greatest number of taxa are temperate elements with Holarctic distribution. Results of ecological analysis showed that in regard to the substratum aspect, terricolous, basophilous, and indifferent species were dominant. In relation to the ecological parameter humidity, most species were mesophilous. The majority of identified bryophytes were sciophilous taxa.

Аnti-neutrophil cytoplasmic antibodies - importance for the diagnosis and the activity assessment of systemic and drug-induced lupus erythematosus

Uvod. Cilj studije je bio da se ispita uloga antineutrofilnih citoplazmatskih antitela (ANCA) i aktivnosti serumske DNaze I kod idiopatskog sistemskog lupus erythematosusa (SLE) i lupus-like sindroma (LLS) indukovanog propiltiouracilom (PTU). Metod. Poređena su 142 pacijenta sa idiopatskim SLE i 17 pacijenata sa LLS indukovanim PTU. Ispitan je ANCA profil (mijeloperoksidaza-MPO, proteinaza 3-PR3, elastaza-El, laktoferin-Lf, katepsin G, BPI-engl. bactericidal/permeability increasing protein), serološki parametri (anti-dsDNK, anti-extractable nuclear antigens (ENA), anti-nukleozomalna, antihistonska, anti-C1q, anti-kardiolipinska antitela, komponente komplementa, krioglobulini) i aktivnost serumske DNaze I kod pacijenata sa SLE i LLS indukovanim PTU. Kontrolne grupe su formirane od zdravih osoba i od pacijenata lečenih PTU ali bez LLS. Klinički i serološki je praćeno 25 ANCA pozitivnih SLE i 12 LLS pacijenata tokom 1.8±0.5 i 4.1±2.0 godina. Rezultati. ANCA su nađena kod 28% pacijenata sa SLE i kod 100% pacijenata sa LLS izazvanim PTU. Pacijenti sa LLS izazvanim PTU znatno češće su imali kutani vaskulitis, a znatno ređe artritis i zahvaćenost bubrega (p<0.01) u odnosu na pacijente sa SLE. ANCA pozitivni SLE pacijenti su češće imali autoimunski hepatitis (p<0.05) i neurološke/psihijatrijske manifestacije (p<0.01), a značajno ređe su imali kutane manifestacije (p<0.05) u odnosu na ANCA negativne pacijente sa SLE. Učestalost hematoloških, nefroloških i artikularnih manifestacija, kao i polioserozitisa, nije se razlikovala kod ANCA pozitivnih i ANCA negativnih pacijenata sa SLE. ANCA su kod pacijenata sa SLE bila monospecifična (77.5%) i to najčešće anti-Lf (52.5%), anti-MPO (47.5%) ili anti-El (15%). Anti-Lf kod pacijenata sa SLE nisu bila udružena sa prisustvom anti-MPO (p<0.01) i anti-El (p<0.05). Kod pacijenata sa LLS indukovanim PTU nađena su polispecifična ANCA (82.4%) i to najčešće anti-MPO (100%), anti-El (82.4%) i anti-PR3 (52.9%)..

Assessment of the chemical composition and in vitro antimicrobial potential of extracts of the liverwort Scapania aspera.

The chemical composition of Scapania aspera extracts was determined by solid phase micro extraction gas chromatography–mass spectrometry (SPME GC-MS) and 96 constituents were identified. The dominant compounds in the methanol extract were β-barbatene (25.1%), o-cymene (14.0%), α-barbatene (5.7%), allo-aromadendrene (4.9%) and β-bourbonene, while in the ethanol extract, o-cymene (17.8%), β-barbatene (17.6%), α-thujene (6.7%), octen-1-ol acetate (4.9%) and β-bazzanene (2.4%) were the major components. In the ethyl acetate extract, β-barbatene (14.3%), undecane (11.8%), 2-methyldecane (11.2%), decane (10.9%) and o-cymene (3.6%) were major components. The antimicrobial activity of the different extracts was evaluated against pathogenic and food spoilage microorganisms using disc diffusion and micro-broth dilution methods. The minimal inhibitory concentration (MIC) of extracts of S. aspera varied from 0.4 to 1.5 mg/mL and 1 to 3 mg/mL for yeast and bacterial strains, respectively. The zone of inhibition of the methanol extract for yeast strains was higher than that for bacterial strains. The results suggest that S. aspera extracts have potential as natural antimicrobial agents

Supplementary data for article: Novakovic, M.; Bukvicki, D.; Andjelkovic, B.; Ilic-Tomic, T.; Veljic, M.; Tesevic, V.; Asakawa, Y. Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia Cruciata. Journal of Natural Products 2019, 82 (4), 694–701. https://doi.org/10.1021/acs.jnatprod.8b00390

Supporting information for: [https://doi.org/10.1021/acs.jnatprod.8b00390]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3096

Supplementary data for article: Ilic-Tomic, T.; Sokovic, M.; Vojnovic, S.; Ciric, A.; Veljic, M.; Nikodinovic-Runic, J.; Novakovic, M. Diarylheptanoids from Alnus Viridis Ssp Viridis and Alnus Glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas Aeruginosa. Planta Medica 2017, 83 (1–2), 117–125. https://doi.org/10.1055/s-0042-107674

Supplementary material for: [https://doi.org/10.1055/s-0042-107674]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2395

Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa

Diarylheptanoids from the barks of Alnus viridis ssp. viridis (green alder) and Alnus glutinosa (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Although all of the investigated compounds negatively influenced the motility of P. aeruginosa PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40-70%. Three of the diarylheptanoids (3, 4, and 5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein in C. violaceum CV026, with 5 being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was shown to significantly decrease the production of acyl homoserine lactones (AHL) in P. aeruginosa PAO1, more specifically, production of the long chain N-(3-oxododecanoyl)- l-HSL. On the other side, four diarylheptanoids (2-5) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of the P. aeruginosa quinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, their in vitro antiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 mu g/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3034

Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata

Seven new bisbibenzyls (1−7) were isolated from the methanol extract of the liverwort Lunularia cruciata along with one previously known bibenzyl and five known bisbibenzyls. The structures of compounds 1−7 were elucidated on the basis of the spectroscopic data. These newly isolated bisbibenzyls may be divided into two groups, the acyclic bisbibenzyls, perrottetins (1− 3), and the cyclic analogues, riccardins (4−7). Besides standard perrottetin and riccardin structures (1 and 4, respectively), they contain phenanthrene (3 and 5), dihydrophenanthrene (2), and quinone moieties (6 and 7), rarely found in natural products. The new compounds 3 and 5, as well as the known riccardin G, exhibited cytotoxic activity against the A549 lung cancer cell line with IC50 values of 5.0, 5.0, and 2.5 μM, respectively.Supporting information: [https://cer.ihtm.bg.ac.rs/handle/123456789/4449

Bis-Bibenzyls from the Liverwort Pellia endiviifolia and Their Biological Activity

Based on previous investigations where bis-bibenzyls isolated from liverworts showed various biological activities (cytotoxic, antimicrobial, and antiviral), we investigated their cytotoxic activity in several human cancer cell lines. From the methylene-chloride/methanol extract of the liverwort Pellia endiviifolia, three bis-bibenzyls of the perrottetin type were isolated, namely perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E. The last two were found for the first time in this species. Their structures were resolved using 1D and 2D NMR, as well as by comparison with data in the literature. Cytotoxic activity of the isolated compounds was tested on three human leukemia cell lines, HL-60 (acute promyelocytic leukemia cells), U-937 (acute monocytic leukemia cells), and K-562 (human chronic myelogenous leukemia cells), as well as on human embryonal teratocarcinoma cell line (NT2/D1) and human glioblastoma cell lines A-172 and U-251, and compared to the previously isolated bis-bibenzyls (perrottetins) of similar structure. The isolated compounds exhibited modest activity against leukemia cells and significant activity against NT2/D1 and A-172. Overall, the most active cytotoxic compounds in this investigation were perrottetin E (1), isolated in this work from Pellia endiviifolia, and perrottetin F phenanthrene derivative (7), previously isolated from Lunularia cruciata and added for a comparison of their cytotoxic activity

Supporting information for: "Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata"

NMR spectra of the isolated compounds and additional figures and tables. Table S1. Elution Program for the Silica Gel Column Separation; Figure S1. Aromatic part of the 1H NMR spectrum of compound 1;This is the Supporting information for the article: Novaković, M., Bukvicki, D., Anđelković, B. D., Ilić-Tomić, T., Veljić, M., Tešević, V.,& Asakawa, Y. (2019). Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia cruciata. Journal of Natural Products, American Chemical Society (ACS), 82(4), 694-701. [https://doi.org/10.1021/acs.jnatprod.8b00390]Published version of the article: [https://cer.ihtm.bg.ac.rs/handle/123456789/2838