Performance Enhancement of Routing in MANETs by using EOMD

Usually large scale of network applications requires communication of the single copy of same information packets simultaneously to many destinations. Applying the infrastructure- based multicast routing protocols in Mobile Ad hoc wireless Networks (MANETs) is a big challenge task. The circumstances that make Multicasting in ad hoc networks is extra intricate than in wired networks are node mobility, Interference of Wi-Fi alerts and broadcast nature of the communication. Tree based Protocols aren't suitable for common topology modifications as an excessive amount of overhead for updating the filter information and additionally no longer suitable for partition or isolation. The major impact of routing for multi-hop MANETs comes due to mobility of the node, as performance is prone to modifications in network topology. When any link breaks, the direction should be repaired or changed, similar to direction preservation or route discovery, respectively. The rerouting process charges in radio bandwidth and battery energy, and the extra routing latency can also affect QoS for community packages, degrading communication performance. The ODMRP is more robust to mobility and unreliable wireless links as its core layout relies on periodic floods of path discovery and renovation. ODMRP periodically reconstructs the ?forwarding mesh? on a fixed quick interval. The path refresh is the most essential parameter because it has the important effect at the protocol overhead. We proposed an Extended - On Demand Multicast Routing Protocol with motion detection (EOMD), which reduces communication overhead and performance improvisation in mobile Ad-Hoc Network in Mobility

Genetic diversity assessment of wild and cultivated varieties of Jatropha curcas (L.) in India by RAPD analysis

The present study deals with evaluation of genetic diversity and pedigree analysis through RAPD analysis. A total number of 40 Jatropha curcas accessions collected from different geographical regions and 43 random decamer primers were screened to assess polymorphism. 10 primers were amplified and 94 polymorphic bands were found out of 125 scored. Accounting for 75.2 % polymorphism across the genotypes 12.5 bands per primer, out of 9.4 were polymorphic. Jaccard’s coefficient of similarity varied from 0.00 to 1.00 indicative of high levels of genetic variation among the genotypes studied. Cluster analysis of data using UPGMA algorithm placed the 40 accessions into 2 main clusters, with cluster II divided into six sub-clusters. The result provides valid guidelines for the collection, conservation and characterization of Jatropha curcas genetic resources

Comparison of leakage rates of methyl bromide and sulfuryl fluoride during structural fumigations

In structural fumigations, half-loss time (HLT) is the most frequently used indicator for comparing fumigant leakage rates. In practical situations where gas leakage rates during structural fumigations are compared, environmental conditions generally are not analyzed in detail and sealing quality is assumed to be constant or fixed. This gives a false impression that a certain gas fumigant might be contained in a structure better than another fumigant. During commercial structural fumigations at the Hal Ross Flour Mill, Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas, USA, leakage characteristics of Methyl bromide (MB) and Sulfuryl fluoride (SF) were compared by taking internal and external environmental conditions into consideration. Two sets of one 24-h MB and one 24-h SF fumigation experiments were conducted in May and August 2009. Mill sealing and fumigations were conducted by two separate commercial fumigators. After sealing, sealing quality prior to a fumigation was verified by a building pressurization test. The mill was subjected to different pressure levels generated by a specially made fan. At each pressure level, the air flow rate through a calibrated fan was measured. The observed air flow rate plotted as a function of pressure quantified leakage characteristics of the mill. In two MB and SF fumigations, gas concentrations were continuously monitored during the entire fumigation period. A weather station was installed on the roof of the mill to monitor outside barometric pressure, wind speed and direction, temperature, and relative humidity. Inside the mill, a temperature and relative humidity data logger was placed on each of the five floors of the mill. Results of this study provided a quantitative side-by-side comparison between MB and SF in the same facility. The pressurization test showed that sealing effectiveness can be quantitatively determined ahead of fumigation. It also confirmed the sealing quality for all fumigations was essentially similar. MB and SF sowed similar gas distribution and leakage characteristics. Although the observed HLTs of the fumigations were different, those differences could be explained by the differences in environmental conditions, primarily wind speed, and to a certain extent mill temperature, rather than inherent properties of MB and SF gases.Keywords: Structural fumigation, Half-loss time, Grain-processing facility, Sulfuryl fluoride, Methyl bromid

A simplified and improved modeling approach for the structural fumigation process using computational fluid dynamics

A 3D Computational fluid dynamics (CFD) model of the fumigation process in the Hal Ross Flour Mill of Kansas State University, Manhattan, Kansas, USA, was formulated for prediction of the gas leakage rate to approximate the gas Half-loss time (HLT) during fumigation with Methyl bromide (MB) and Sulfuryl fluoride (SF). The model consisted of external and internal flow domains. The external domain was used to predict stagnation pressures generated by wind impinging on the mill’s walls. The internal domain was used to predict fumigant leakage rates in terms of HLT. Cracks on the mill’s walls represented the effective leakage areas on the internal flow domain. This modeling approach had been used by the authors (Chayaprasert and Maier) in a previous study, but it was simplified and improved in the present study. The primary simplification in the modeling approach was exclusion of the flour mill’s interior details (e.g., milling equipment), reducing the model formulation and simulation computing times. In the previous study, the gas-tightness of the internal flow domain was identified by varying the flow resistance coefficient of the effective leakage areas until the model yielded a HLT value that was close to the one observed from the experimental fumigant concentration data. In the present study, the domain gas-tightness was verified by building pressurization tests. The model was validated using data from one MB and one SF fumigation experiments. The HLTs provided by simulated fumigations were in good agreement with those determined from the experiments. The result of the present study provides further validation to the modeling approach and emphasizes the importance of building pressurization test for accurate HLT prediction. Keywords: Structural fumigation, Half-loss time, Pilot flour mill, Computational Fluid Dynamics (CFD), Building pressurization tes

Diagnostic Molecular Markers for Phosphine Resistance in US Populations of Tribolium castaneum and Rhyzopertha dominica

Citation: Chen, Z., Schlipalius, D., Opit, G., Subramanyam, B., & Phillips, T. W. (2015). Diagnostic Molecular Markers for Phosphine Resistance in US Populations of Tribolium castaneum and Rhyzopertha dominica. Plos One, 10(3), 14. doi:10.1371/journal.pone.0121343Stored product beetles that are resistant to the fumigant pesticide phosphine (hydrogen phosphide) gas have been reported for more than 40 years in many places worldwide. Traditionally, determination of phosphine resistance in stored product beetles is based on a discriminating dose bioassay that can take up to two weeks to evaluate. We developed a diagnostic cleaved amplified polymorphic sequence method, CAPS, to detect individuals with alleles for strong resistance to phosphine in populations of the red flour beetle, Tribolium castaneum, and the lesser grain borer, Rhyzopertha dominica, according to a single nucleotide mutation in the dihydrolipoamide dehydrogenase (DLD) gene. We initially isolated and sequenced the DLD genes from susceptible and strongly resistant populations of both species. The corresponding amino acid sequences were then deduced. A single amino acid mutation in DLD in populations of T. castaneum and R. dominica with strong resistance was identified as P45S in T. castaneum and P49S in R. dominica, both collected from northern Oklahoma, USA. PCR products containing these mutations were digested by the restriction enzymes MboI and BstNI, which revealed presence or absence, respectively of the resistant (R) allele and allowed inference of genotypes with that allele. Seven populations of T. castaneum from Kansas were subjected to discriminating dose bioassays for the weak and strong resistance phenotypes. Application of CAPS to these seven populations confirmed the R allele was in high frequency in the strongly resistant populations, and was absent or at a lower frequency in populations with weak resistance, which suggests that these populations with a low frequency of the R allele have the potential for selection of the strong resistance phenotype. CAPS markers for strong phosphine resistance will help to detect and confirm resistant beetles and can facilitate resistance management actions against a given pest population

High sensitivity C-reactive protein levels across spectrum and severity of coronary artery disease

Background: C-reactive protein (CRP) is an acute-phase reactant protein synthesized by the liver in response to acute\ud stress in a wide range of acute and chronic inflammatory conditions. In healthy subjects and patients presenting with\ud coronary artery disease (CAD), elevated levels of CRP has repeatedly been demonstrated to predict future cardiovascular\ud events.\ud Methods: We measured high sensitivity C-reactive protein (hs-CRP) levels in 382 consecutive patients with CAD and 60 healthy controls by immunoturbidimetry method. Risk factors like hypertension, diabetes mellitus, dyslipidaemia,smoking, obesity and family history of premature CAD were assessed.\ud Results: The mean age of patients with CAD was 53.5±11.8 years (303 males) and that of control group was 50.83±8.07(28 males). The patient group had significant higher concentration of mean hs-CRP levels when compared\ud with the healthy control group (1.8±1.9 mg/L vs 0.35±1.1 mg/L, p<0.001). The mean hs-CRP levels of unstable angina\ud (USA) and myocardial infarction (MI) patients was higher than chronic stable angina (CSA) patients (p<0.05). Based\ud on the disease severity, we found a significantly higher hs-CRP levels in patients of triple vessel disease when compared\ud to patients with single vessel disease (p=0.01).\ud Conclusions: Elevated serum hs-CRP levels provide a useful marker for cardiovascular risk which, when combined\ud with traditional risk factors, may help improve global risk prediction. Our study showed a significant contribution of\ud hs-CRP to coronary risk prediction with better discrimination

Biological effects of power frequency magnetic fields: Neurochemical and toxicological changes in developing chick embryos

BACKGROUND: There are several reports that indicate a linkage between exposure to power frequency (50 – 60 Hz) magnetic fields with abnormalities in the early embryonic development of the chicken. The present study was designed to understand whether power frequency electromagnetic fields could act as an environmental insult and invoke any neurochemical or toxicological changes in developing chick embryo model. METHODS: Fertilized chicken eggs were subjected to continuous exposure to magnetic fields (50 Hz) of varying intensities (5, 50 or 100 μT) for a period of up to 15 days. The embryos were taken out of the eggs on day 5, day 10 and day 15. Neurochemical (norepinephrine and 5-hydroxytryptamine) and amino acid (tyrosine, glutamine and tryptophan) contents were measured, along with an assay of the enzyme glutamine synthetase in the brain. Preliminary toxicological investigations were carried out based on aminotransferases (AST and ALT) and lactate dehydrogenase activities in the whole embryo as well as in the liver. RESULTS: The study revealed that there was a significant increase (p < 0.01 and p < 0.001) in the level of norepinephrine accompanied by a significant decrease (p < 0.01 and p < 0.001) in the tyrosine content in the brain on day 15 following exposure to 5, 50 and 100 μT magnetic fields. There was a significant increase (p < 0.001) in glutamine synthetase activity resulting in the significantly enhanced (p < 0.001) level of glutamine in the brain on day 15 (for 100 μT only). The possible mechanisms for these alterations are discussed. Further, magnetic fields had no effect on the levels of tryptophan and 5-hydroxytryptamine in the brain. Similarly, there was no effect on the activity of either aminotransferases or lactate dehydrogenase in the whole embryo or liver due to magnetic field exposure. CONCLUSIONS: Based on these studies we conclude that magnetic field-induced changes in norepinephrine levels might help explain alterations in the circadian rhythm, observed during magnetic field stress. Also, the enhanced level of glutamine can act as a contributing factor for developmental abnormalities

Serum selenium concentration and antioxidant activity in cervical cancer patients before and after treatment

Aim - in the present study, the effect of chemo and radio therapies on serum trace elements content and antioxidant activity in blood serum of cervical cancer patients was evaluated. Among 104 cervical cancer patients selected for the present study, 54 and 50 patients were treated with chemo- and radiotherapy respectively. Plasma Se, Zn, Cu and some enzymatic antioxidants activities were estimated in serum before and after the treatment. The decreased levels of serum trace elements, glutathione peroxidase activity and total antioxidant capacity, and increased malondialdehyde, glutathion reductase was observed in cervical cancer patients when compared to healthy controls. The increased concentration of serum Se, Zn was observed in patients treated with chemotherapy. Simultaneously there was a significant (P << 0,001) increase in glutathione peroxidase and total antioxidant capacity, and significant decrease (P << 0,05) in malondialdehyde and glutathion reductase levels in the serum of patients treated with chemotherapy compared to the patients treated with radiotherapy. Key Words: cervical cancer, free radicals, glutathione peroxidase, selenium, chemotherapy, radiotherapy

Serum selenium concentration and antioxidant activity in cervical cancer patients before and after treatment

Aim - in the present study, the effect of chemo and radio therapies on serum trace elements content and antioxidant activity in blood serum of cervical cancer patients was evaluated. Among 104 cervical cancer patients selected for the present study, 54 and 50 patients were treated with chemo- and radiotherapy respectively. Plasma Se, Zn, Cu and some enzymatic antioxidants activities were estimated in serum before and after the treatment. The decreased levels of serum trace elements, glutathione peroxidase activity and total antioxidant capacity, and increased malondialdehyde, glutathion reductase was observed in cervical cancer patients when compared to healthy controls. The increased concentration of serum Se, Zn was observed in patients treated with chemotherapy. Simultaneously there was a significant (P << 0,001) increase in glutathione peroxidase and total antioxidant capacity, and significant decrease (P << 0,05) in malondialdehyde and glutathion reductase levels in the serum of patients treated with chemotherapy compared to the patients treated with radiotherapy

Synthesis, spectral characterization and anti-diabetic activity evaluation of phosphoramidate derivatives

60-68In the present work, a series of new phosphoramidate derivatives have been conveniently synthesized by using 4- chlorobenzene-sulfonyl chloride as preliminary material via substituted sulfonamides as intermediates with high yields in a short period by conventional and microwave irradiation techniques. All the synthesized compound have been characterized by spectral and elemental analyses. An in silico molecular docking study has been performed to find potent anti-diabetic drugs. In vitro anti-diabetic activity of the title compounds have also been screened by standard α-amylase inhibition assay. Some of the tested compounds have been proven to possess promising activity when compared with reference drug