Evaluating Recruitment of American Eel, Anguilla rostrata, to the Potomac River Spring 2004

The Atlantic States Marine Fisheries Commission (ASMFC) adopted the Interstate Fishery Management Plan (FMP) for the American eel in November 1999. The FMP focuses on increasing the states’ efforts to collect data on the resource and the fishery it supports through both fishery dependent and fishery independent studies. To this end, member jurisdictions (including the PRFC) agreed to implement an annual abundance survey for young of year (YOY) American eels. The survey is intended to “…characterize trends in annual recruitment of young of year eels over time [to produce a] qualitative appraisal of the annual recruitment of American eel to the U.S. Atlantic coast (ASMFC, 2000). The development of these surveys began as pilot surveys in 2000 with full implementation by the 2001 season. Results from these surveys will provide critical data on eel coastal recruitment success and further understanding of American eel population dynamics

Evaluating Recruitment of American Eel, Anguilla rostrata, to the Potomac River (Spring 2006)

Fisheries management techniques are not often applied to American eels because basic biological information is not well known. Unknown biological parameters such as variation in growth rates and length at age have complicated stock assessment and management efforts. Though American eel are not usually considered a sport fish, their ubiquity and readiness to take a bait leads them to be caught by recreational fishermen (Collette and Klein-MacPhee, 2002). Young American eel are also used as a baitfish in coastal areas (Jenkins and 4 Burkhead, 1993.) Absence of basic population dynamics data has hampered attempts at evaluation of regional exploitation rates (Social Research for Sustainable Fisheries, 2002). Additionally, relatively few studies have addressed the recruitment of glass eels to Atlantic coast estuaries from the Sargasso Sea spawning grounds. The Atlantic States Marine Fisheries Commission (ASMFC) adopted the Interstate Fishery Management Plan (FMP) for the American eel in November 1999. The FMP focuses on increasing the states’ efforts to collect data on American eel and the fishery it supports through both fishery dependent and fishery independent studies. To this end, member jurisdictions (including the PRFC) agreed to implement an annual abundance survey for young of year (YOY) American eels. The survey is intended to “…characterize trends in annual recruitment of young of year eels over time [to produce a] qualitative appraisal of the annual recruitment of American eel to the U.S. Atlantic coast (ASMFC, 1999). These surveys began as pilot surveys in 2000 with full implementation by the 2001 season. Survey results will provide critical data on eel coastal recruitment success and further understanding of American eel population dynamics

Estimating Relative Abundance of Young of Year American Eel, Anguilla rostrata, in the Virginia Tributaries of Chesapeake Bay (Spring 2005)

The Atlantic States Marine Fisheries Commission (ASMFC) adopted the Interstate Fishery Management Plan (hereafter referred to as FMP) for the American eel in November 1999. The FMP focuses on increasing coastal states’ efforts to collect American eel data through both fishery dependent and fishery independent studies. Consequently, member jurisdictions (including Virginia) agreed to implement an annual survey for YOY American eels. The survey is intended to “…characterize trends in annual recruitment of the YOY eels over time [to produce a] qualitative appraisal of the annual recruitment of American eel to the U.S. Atlantic Coast” (ASMFC, 2000). The development of these surveys began in 2000 with full implementation by 2001. Survey results should provide necessary data on coastal recruitment success and further understanding of American eel population dynamics. A recent American eel stock assessment report (ASMFC, 2006) emphasized the importance of the coast-wide survey as an index of sustained recruitment over the historical coastal range and an early warning of potential range contraction of the species

Estimating Relative Abundance of Young of Year American Eel, Anguilla rostrata, in the Virginia Tributaries of Chesapeake Bay (Spring 2004 Survey)

The Atlantic States Marine Fisheries Commission (ASMFC) adopted the Interstate Fishery Management Plan (hereafter referred to as FMP) for the American eel in November 4 1999. The FMP focuses on increasing coastal states’ efforts to collect American eel data through both fishery dependent and fishery independent studies. Consequently, member jurisdictions (including Virginia) agreed to implement an annual survey for young of year (YOY) American eels. The survey is intended to “…characterize trends in annual recruitment of the young of year eels over time [to produce a] qualitative appraisal of the annual recruitment of American eel to the U.S. Atlantic Coast” (ASMFC, 2000). The development of these surveys began in 2000 with full implementation by 2001. Survey results will provide necessary data on coastal recruitment success and further the understanding of American eel population dynamics

Estimating Relative Abundance of Young of Year American Eel, Anguilla rostrata, in the Virginia Tributaries of Chesapeake Bay. Final Report (2003 Reporting Year)

The Atlantic States Marine Fisheries Commission (ASMFC) adopted the Interstate Fishery Management Plan (hereafter referred to as FMP) for the American Eel in November 1999. The FMP focuses on increasing the state’s efforts to collect data on the resource and the fishery it supports through both fishery-dependent and fishery-independent studies. To this end, member jurisdictions (including Virginia) agreed to implement an annual abundance survey for young of year (YOY) American eels. The survey is intended to “…characterize trends in annual recruitment of the young of year eels over time [to produce a] qualitative appraisal of the annual recruitment of American eel to the U.S. Atlantic Coast (ASMFC, 2000). The development of these surveys began as pilot surveys in 2000 with full implementation by the 2001 season. Results from these surveys will provide necessary data on coastal recruitment success and further the understanding of American eel population dynamic

Efficacy of blue crab spawning sanctuaries in Chesapeake Bay

Sanctuaries can potentially protect a significant fraction of the spawning stock, and thereby sustain heavily exploited populations. Despite the worldwide use of marine and estuarine spawning sanctuaries, the effectiveness of such sanctuaries remains untested. We therefore attempted to quantify the effectiveness of the spawning sanctuaries for adult female blue crabs (Callinectes sapidus) in Chesapeake Bay. We used baywide winter dredge survey data to estimate the potential spawning stock prior to the major exploitation period, and summer trawl survey data to estimate spawning stock abundance within the Lower Bay Spawning Sanctuary and adjacent Bayside Eastern Shore Sanctuary during the reproductive period. Hence, we were able to approximate the percentage of the potential spawning stock that was protected by both sanctuaries after exploitation. On average, approximately 16% of the potential spawning stock survived to reach the Lower Bay Spawning Sanctuary and Bayside Eastern Shore Sanctuary. Even under a best-case scenario (i.e., crab residence time of 2 weeks), the sanctuaries only protected an estimated 22% of the potential spawning stock, which is well below the percentage recommended by recent stock assessments for sustainable exploitation (28%). In the worst case, a mere 11% of the potential spawning stock survived to reach the spawning sanctuaries. Hence, we recommend a substantial expansion of the spawning sanctuaries, as well as the complementary protection of other life stages in critical habitats, such as nursery grounds and dispersal corridors. Furthermore, traditional fisheries management measures (e.g., effort control) should be used in concert with sanctuaries to thwart impediments to effective implementation of the sanctuaries, such as redirected fishing effort.https://scholarworks.wm.edu/vimsbooks/1084/thumbnail.jp

A Deepwater Dispersal Corridor for Adult Female Blue Crabs in Chesapeake Bay

In marine ecosystems, there is no empirical evidence for the utility of dispersal corridors in conservation, despite widespread migrations by mammals, fish, and invertebrates. We investigated the potential for a deepwater dispersal corridor (\u3e 13 m depths) in protecting adult females of the blue crab, Callinectes sapidus, en route from shallow-water nursery and mating areas to the spawning sanctuary in lower Chesapeake Bay.https://scholarworks.wm.edu/vimsbooks/1128/thumbnail.jp

An alternative application to the portuguese agro-industrial residue : wheat straw

The effects of alkaline treatments of the wheat straw with sodium hydroxide were investigated. The optimal condition for extraction of hemicelluloses was found to be with 0.50 mol/l sodium hydroxide at 55 °C for 2 h. This resulted in the release of 17.3% of hemicellulose (% dry starting material), corresponding to the dissolution of 49.3% of the original hemicellulose. The yields were determined by gravimetric analysis and expressed as a proportion of the starting material. Chemical composition and physico-chemical properties of the samples of hemicelluloses were elucidated by a combination of sugar analyses, Fourier transform infrared (FTIR), and thermal analysis. The results showed that the treatments were very effective on the extraction of hemicelluloses from wheat straw and that the extraction intensity (expressed in terms of alkali concentration) had a great influence on the yield and chemical features of the hemicelluloses. The FTIR analysis revealed typical signal pattern for the hemicellulosic fraction in the 1,200–1,000 cm−1 region. Bands between 1,166 and 1,000 cm−1 are typical of xylans.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo a Pesquisa do Estado de São Paulo/Brazil (FAPESP)Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BPD/26156/2005, SFRH/BPD/26108/200

Loss of the Wnt/β-catenin pathway in microglia of the developing brain drives pro-inflammatory activation leading to white matter injury

Microglia-mediated neuroinflammation is key in numerous brain diseases including encephalopathy of the preterm born infant. Microglia of the still-developing brain have unique properties but little is known of how they regulate their inflammatory activation. This is important information as every year 9 million preterm born infants acquire persisting neurological injuries associated with encephalopathy and we lack strategies to prevent and treat these injuries. Our study of activation state regulators in immature brain microglia found a robust down-regulation of Wnt/β-catenin pathway receptors, ligands and intracellular signalling members in pro-inflammatory microglia. We undertook our studies initially in a mouse model of microglia-mediated encephalopathy including the clinical hallmarks of oligodendrocyte injury and hypomyelination. We purified microglia from this model and applied a genome-wide transcriptomics analysis validated with quantitative profiling. We then verified that down-regulation of the Wnt/β-catenin signalling cascade is sufficient and necessary to drive microglia into an oligodendrocyte-damaging phenotype using multiple pharmacological and genetic approaches in vitro and in vivo in mice and in humans and zebrafish. We also demonstrated that genomic variance in the WNT/β-catenin pathway is associated with the anatomical connectivity phenotype of the human preterm born infant. This integrated analysis of genomics and connectivity, as a surrogate for oligodendrocyte function/myelination, is agnostic to cell type. However, this data indicates that the WNT pathway is relevant to human brain injury and specifically that WNT variants may be useful clinically for injury stratification and prognosis. Finally, we performed a translational experiment using a BBB penetrant microglia-specific targeting 3DNA nanocarrier to deliver a Wnt agonist specifically and directly to microglia in vivo. Increasing the activity of the Wnt/β-catenin pathway specifically in microglia in our model of microglia-mediated encephalopathy was able to reduce microglial pro-inflammatory activation, prevent the typical hypomyelination and also prevent the long-term memory deficit associated with this hypomyelination. In summary, the canonical Wnt/β-catenin pathway regulates microglial activation and up-regulation of this pathway could be a viable neurotherapeutic strategy

Omega-3 polyunsaturated fatty acids favourably modulate cardiometabolic biomarkers in type 2 diabetes: a meta-analysis and meta-regression of randomized controlled trials

BACKGROUND: Randomized controlled trials (RCTs) suggest that supplementation with omega-3 polyunsaturated fatty acids (n-3PUFAs) may favourably modify cardiometabolic biomarkers in type 2 diabetes (T2DM). Previous meta-analyses are limited by insufficient sample sizes and omission of meta-regression techniques, and a large number of RCTs have subsequently been published since the last comprehensive meta-analysis. Updated information regarding the impact of dosage, duration or an interaction between these two factors is therefore warranted. The objective was to comprehensively assess the effect of n-3PUFAs supplementation on cardiometabolic biomarkers including lipid profiles, inflammatory parameters, blood pressure, and indices of glycaemic control, in people with T2DM, and identify whether treatment dosage, duration or an interaction thereof modify these effects. METHODS: Databases including PubMed and MEDLINE were searched until 13th July 2017 for RCTs investigating the effect of n-3PUFAs supplementation on lipid profiles, inflammatory parameters, blood pressure, and indices of glycaemic control. Data were pooled using random-effects meta-analysis and presented as standardised mean difference (Hedges g) with 95% confidence intervals (95% CI). Meta-regression analysis was performed to investigate the effects of duration of supplementation and total dosage of n-3PUFAs as moderator variables where appropriate. RESULTS: A total of 45 RCTs were identified, involving 2674 people with T2DM. n-3PUFAs supplementation was associated with significant reductions in LDL [ES: - 0.10, (95% CI - 0.17, - 0.03); p = 0.007], VLDL (ES: - 0.26 (- 0.51, - 0.01); p = 0.044], triglycerides (ES: - 0.39 (- 0.55, - 0.24; p ≤ 0.001] and HbA1c (ES: - 0.27 (- 0.48, - 0.06); p = 0.010]. Moreover, n-3PUFAs supplementation was associated with reduction in plasma levels of TNF-α [ES: - 0.59 (- 1.17, - 0.01); p = 0.045] and IL-6 (ES: - 1.67 (- 3.14, - 0.20); p = 0.026]. All other lipid markers, indices of glycaemic control, inflammatory parameters, and blood pressure remained unchanged (p > 0.05). CONCLUSIONS: n-3PUFAs supplementation produces favourable hypolipidemic effects, a reduction in pro-inflammatory cytokine levels and improvement in glycaemia. Neither duration nor dosage appear to explain the observed heterogeneity in response to n-3PUFAs. Trial registration This trial was registered at http://www.crd.york.ac.uk as CRD42016050802