Parasitological Contamination of Wastewater Irrigated and Raw Manure Fertilized Vegetables in Mekelle City and Its Suburb, Tigray, Ethiopia

Watering of vegetables in developing countries including Ethiopia is done using untreated wastewater and raw manure of domestic animal origin is used as fertilizer. Thus, vegetables are considered to be the principal  sources of human infection with bacterial, protozoan and helminth parasites. Therefore, the objective of this study was to evaluate the rate of parasitological contamination of pre-harvest vegetables in Mekelle city and its suburban village, ‘Mariam Dahan’. Pre-harvest vegetables were collected from the field during the dry season, washed using physiological saline solution, allowed to sediment overnight, centrifuged and examined microscopically for infective stages of intestinal parasites. Out of the  vegetable samples examined, 32.41% in Mekelle city and 30.49% in  ‘Mariam Dahan’, contained at least one parasitic contaminant. Strongyloides stercoralis was most encountered parasitic contaminant in the study areas, followed by, Taenia and Entamoeba spp. Highest rate of parasitic contamination was detected on Swiss chard, that was 45.5 and 41.67% in Mekelle city and ‘Mariam Dahan’, respectively. Occurrence of infective stages of intestinal parasites on wastewater-irrigated vegetables may pose public health hazards to farming communities in the study  areas. Therefore, evaluation and surveillance of parasitological quality of vegetables is crucial in an attempt to control vegetable-transmitted  parasitic infections.Keywords: Untreated wastewater, Raw manure, Pre-harvest Vegetables, Parasitological contamination, Tigray, Ethiopi

Knowledge, Attitude and Practice (KAP) about Insecticide Treated Net (ITN) usage against Malaria in Kolla Tembien district, Tigray, Ethiopia

Optimal use of insecticide treated nets (ITNs) to prevent malaria in a community depends on vector behavior, mass distribution, knowledge and willingness of people to use the nets. This study was aimed at evaluating knowledge, attitude and practice (KAP) about ITNs at household level in rural sub-distracts of Kolla Tembien. For this purpose, interview questionnaire containing KAP variables were administered to the randomly selected household heads. A total of 368 households were surveyed, and 70.10 % of the respondents were females. The X number of occupants in the households surveyed was found to be 5.50. However, the X number of ITNs owned by the households was found to be 1.07. Most of the respondents (82.34%) indicated ITNs prevent malaria through protection from mosquito bites. Seventy six percent of the respondents claimed children 0.05). Out of the 260 nets inspected for usage and physical condition during the survey period, more than 50% were kept in a box or other container in the households. Nets that were graded as clean and very dirty accounted for 25% and 25.77%, respectively. Physical inspection of the randomly sampled nets also revealed holes ranging from those that didn’t allow a thumb to pass through ( X number = 3.47) to those that were wider than a closed fist ( X number = 1.64). The over all knowledge, attitude and practice of ITN usage was found to be satisfactory in the study district. However, the occupant net ratio must be narrowed and attention needs to be given to malaria vulnerable groups in the household. Continuous monitoring and evaluation of net usage after distribution should be instituted to avoid misuses and associated net damages in the study areas

Genetic diversity in Ethiopian Durum wheat ( Triticum turgidum var durum ) inferred from phenotypic variations

The valorization of genetic diversities of major crops like wheat may help substantially to feed the world Population. Durum heat genotypes consisting of 265 farmers’ varieties (FVs), which have been cultivated for many centuries in Ethiopia, as well as 24 improved varieties (IMVs) have been recently evaluated in northern Ethiopia. The evaluation has been carried out at two different locations for 2 consecutive years to verify the inherited diversity in FVs for important phenological and agronomic traits; with the intention to provide refined information to breeders and genebank managers. As a result of a careful evaluation, a very significant variation was observed between the FVs and IMVs. A large number of the former have demonstrated superior performance to the latter in terms of mean values of the major traits within the stipulated years and locations. The best perform- ing FV has shown a gain of 20% grain yield over the best IMV. Multivariate analyses revealed that FVs displayed larger genetic diversity than in those IMVs. FVs could therefore be used as donor of useful alleles in durum wheat breeding for improvement of yield per se and other traits of agronomic and phenological importance. The identified stable superior FVs include: 8208, 226834A, 238567, 222426, 226282 could be best candidates for farmers in marginal environments. Genotypes that have shown stable performance for spatial variation such as 204493A, 214357 and 238567; and temporal variation such as 8208, 208479, 214357 and 226834A could be the best candidates for exploitation in future breeding programs

The Jus Ad Bellum and the 1998 Initiation of the Eritrean-Ethopian War

From May 1998 to December 2000, Eritrea and Ethiopia engaged in an armed conflict that cost the lives of thousands of individuals, injured thousands more, and displaced tens of thousands of men, women, and children from their homes. In December 2000, the two sides concluded a comprehensive agreement that ended the war. Among other things, the agreement established the Eritrea-Ethiopia Claims Commission. Consisting of five arbitrators, the Commission’s mandate was to “decide through binding arbitration all claims for loss, damage or injury by one Government against the other” that were “related to the conflict” and that “resulted from violations of international humanitarian law, including the 1949 Geneva Conventions, or other violations of international law.” The two countries filed claims with the Commission in December 2001 and from that time until August 2009, the Commission issued seventeen arbitral awards and eight decisions, covering a broad array of claims, including inhumane treatment of prisoners of war and civilian internees, abuse of enemy aliens in a belligerent’s territory or in occupied territory, wrongful seizure of the enemy’s public or private property, indiscriminate battlefield conduct or aerial bombing, harassment of diplomats and seizure of diplomatic property, and many other matters. The book LITIGATING WAR: ARBITRATION OF CIVIL INJURY BY THE ERITREA-ETHIOPIA CLAIMS COMMISSION seeks to integrate in discrete chapters the Commission’s findings on key topics, with each chapter organized into sub-sections that deal with the principal elements of that topic. The guiding emphasis is not on who-filed-what claim but is instead on what kinds of violations were addressed by the Commission, what kinds of evidence were relevant in establishing or defending against such violations, what legal conclusions emerged in addressing those violations, and what levels of compensation were deemed appropriate when a violation was found.The dominant area of international law upon which claims before the Eritrea-Ethiopia Claims Commission were based was the jus in bello, or the law operating as between two belligerents after an armed conflict has arisen. One type of claim filed before the Commission, however, was quite different, in that it concerned an alleged violation of the jus ad bellum, or the law on when a state may resort to a use of military force against another state. As one of the most important norms for the international legal system, the Commission’s treatment of the jus ad bellum claim is of particular interest, and is addressed in the book’s Chapter IV on “Initiation of War.

Molecular detection and phylogenetic analysis of Peste des petits ruminants virus circulating in small ruminants in eastern Amhara region, Ethiopia

Background: Peste des Petits Ruminants (PPR) is a severe, highly infectious and fatal viral disease of small ruminants. Four lineages of PPR virus have been identified globally based on sequence analysis of the nucleoprotein (N) and fusion (F) gene. The aim of this study was to isolate and genetically characterize recently circulating PPR virus in small ruminants in the eastern Amhara region in Ethiopia. A total of 28 anti-mortem samples (gum debris, nasal and ocular swab) were collected from clinically suspicious animals and examined for the presence of PPRV by a one-step RT-PCR assay. Samples positive with RT-PCR were subjected to isolation of the virus which were subsequently genetically characterized by sequencing of the nucleoprotein (N) gene and phylogenetic analysis of PPR virus (PPRV) strains. Results: Of the 28 clinical samples examined, 46.4% were positive with RT-PCR for viral nucleic acid. The PPRV was successfully isolated on CHS-20 cell line with the ovine signaling lymphocyte activation molecule (SLAM) receptor expressed on the cell surface and confirmed with RT-PCR and IFAT assay. The nucleotide sequence and phylogenetic analysis indicated that the PPRV obtained were clustered genetically with Lineage IV isolates of the virus. Conclusion: The successful isolation of the virus and molecular findings of this study confirmed active lineage IV PPRV infections among populations of sheep and goats in eastern Amhara, suggesting risks for potential spread of the disease to currently free areas. Thus, we recommend systematic vaccination to contain outbreaks in affected districts and geographically linked surrounding districts to which the disease could potentially spread due to different epidemiological linkages

Orientation Invariant ECG-Based Stethoscope Tracking for Heart Auscultation Training on Augmented Standardized Patients

Auscultation, the act of listening to the heart and lung sounds, can reveal substantial information about patients’ health and other cardiac-related problems; therefore, competent training can be a key for accurate and reliable diagnosis. Standardized patients (SPs), who are healthy individuals trained to portray real patients, have been extensively used for such training and other medical teaching techniques; however, the range of symptoms and conditions they can simulate remains limited since they are only patient actors. In this work, we describe a novel tracking method for placing virtual symptoms in correct auscultation areas based on recorded ECG signals with various stethoscope diaphragm orientations; this augmented reality simulation would extend the capabilities of SPs and allow medical trainees to hear abnormal heart and lung sounds in a normal SP. ECG signals recorded from two different SPs over a wide range of stethoscope diaphragm orientations were processed and analyzed to accurately distinguish four different heart auscultation areas, aortic, mitral, pulmonic and tricuspid, for any stethoscope’s orientation. After processing the signals and extracting relevant features, different classifiers were applied for assessment of the proposed method; 95.1% and 87.1% accuracy were obtained for SP1 and SP2, respectively. The proposed system provides an efficient, non-invasive, and cost efficient method for training medical practitioners on heart auscultation

High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding

Durum wheat (Triticum turgidum subsp. durum) is a key crop worldwide, and yet, its improvement and adaptation to emerging environmental threats is made difficult by the limited amount of allelic variation included in its elite pool. New allelic diversity may provide novel loci to international crop breeding through quantitative trait loci (QTL) mapping in unexplored material. Here, we report the extensive molecular and phenotypic characterization of hundreds of Ethiopian durum wheat landraces and several Ethiopian improved lines. We test 81 587 markers scoring 30 155 single nucleotide polymorphisms and use them to survey the diversity, structure, and genome-specific variation in the panel. We show the uniqueness of Ethiopian germplasm using a siding collection of Mediterranean durum wheat accessions. We phenotype the Ethiopian panel for ten agronomic traits in two highly diversified Ethiopian environments for two consecutive years and use this information to conduct a genome-wide association study. We identify several loci underpinning agronomic traits of interest, both confirming loci already reported and describing new promising genomic regions. These loci may be efficiently targeted with molecular markers already available to conduct marker-assisted selection in Ethiopian and international wheat. We show that Ethiopian durum wheat represents an important and mostly unexplored source of durum wheat diversity. The panel analysed in this study allows the accumulation of QTL mapping experiments, providing the initial step for a quantitative, methodical exploitation of untapped diversity in producing a better wheat

<i>miniPixD</i>: a compact sample analysis system which combines X-ray imaging and diffraction

This paper introduces miniPixD: a new, compact system that utilises transmission X-ray imaging and X-ray diffraction (XRD) to locate and identify materials of interest within an otherwise opaque volume. The system and the embodied techniques have utility in security screening, medical diagnostics, non-destructive testing (NDT) and quality assurance (QA). This paper outlines the design of the system including discussion on the choice of components and presents some data from relevant samples which are compared to other energy dispersive and angular dispersive XRD techniques

Mechanism for deep crustal seismicity: Insight from modeling of deformation process at the Main Ethiopian Rift

We combine numerical modeling of lithospheric extension with analysis of seismic moment release and earthquake b-value in order to elucidate the mechanism for deep crustal seismicity and seismic swarms in the Main Ethiopian Rift (MER). We run 2-D numerical simulations of lithospheric deformation calibrated by appropriate rheology and extensional history of the MER to simulate migration of deformation from mid-Miocene border faults to ∼30 km wide zone of Pliocene to recent rift floor faults. While currently the highest strain rate is localized in a narrow zone within the rift axis, brittle strain has been accumulated in a wide region of the rift. The magnitude of deviatoric stress shows strong variation with depth. The uppermost crust deforms with maximum stress of 80 MPa, at 8–14 km depth stress sharply decreases to 10 MPa and then increases to a maximum of 160 MPa at ∼18 km depth. These two peaks at which the crust deforms with maximum stress of 80 MPa or above correspond to peaks in the seismic moment release. Correspondingly, the drop in stress at 8–14 km correlates to a low in seismic moment release. At this depth range, the crust is weaker and deformation is mainly accommodated in a ductile manner. We therefore see a good correlation between depths at which the crust is strong and elevated seismic deformation, while regions where the crust is weaker deform more aseismically. Overall, the bimodal depth distribution of seismic moment release is best explained by the rheology of the deforming crust

ECONOMIC PERFORMANCE OF COMMUNITY BASED BEAN SEED PRODUCTION AND MARKETING IN THE CENTRAL RIFT VALLEY OF ETHIOPIA

Limited access to seed of improved varieties is an impediment to agricultural productivity in sub-Saharan Africa. Researchers in the national and international agricultural research systems have been piloting a community based seed multiplication and marketing enterprises (CBSME) model, as an alternative to the formal seed systems, in order to increase availability and accessibility to quality seed of improved common bean ( Phaseolus vulvaris L.) varieties by smallholder farmers. The objective of this study was to assess the profitability of CBSME as an enterprise for seed production and analyse factors that influence farmers\u2019 decisions to participate in it as seed producers or buyers of seed. Gross margins were computed to assess value addition at farm level; while Tobit and multivariate probit models used to respectively, analyse determinants of participation in community based seed multiplication enterprise and its use by producers as a seed source. The community based seed multiplication enterprises were found to be profitable, generating US$792 as gross margins and accessible to farmers for the bean seed, along other seed sources, i.e. formal and informal seed systems. These three seed production and delivery models competed at farm level, but complemented each other in terms of reaching users in different social groups and locations. Community based seed multiplication enterprises as sources of seed were used by farmers located in rural areas and those in farmer organisations/cooperatives. However, seed production through this model is concentrated closer to urban areas, where individual seed producers are easily linked to the formal seed system. This, however, makes the marketing of seed reliant on big buyers for redistribution among remote farming communities.L\u2019acc\ue8s limit\ue9 aux semences de vari\ue9t\ue9s am\ue9lior\ue9es est un obstacle \ue0 la productivit\ue9 agricole en Afrique sub-Saharienne. Les cherheurs dans les systems nationaux de recherches agricoles sont entrain de tester des mod\ue8les d\u2019entreprises communautaires de multiplication et de commercialisation (CBSME); comme une alternative aux syst\ue8mes semenciers formels, dans le but d\u2019accro\ueetre la disponibilit\ue9 et l\u2019accessibilit\ue9 aux semences de qualit\ue9 de l\u2019haricot commun ( Phaseolus vulvaris L.) par les petits producteurs. L\u2019objectif de cette \ue9tude \ue9tait d\u2019\ue9valuer la profitabilit\ue9 de CBSME comme une entreprise de production des semences et d\u2019analyser les facteurs qui influencent les d\ue9cisions des producteurs \ue0 y participer en tant que producteurs et acheteurs de semences. Les marges brutes \ue9taient calcul\ue9es pour \ue9valuer la value ajout\ue9e au niveau de l\u2019exploitation agricole, tandis que les mod\ue8les de Tobit et multivari\ue9 de Probit \ue9taient utilis\ue9s respectivelement, pour analyser les determinants de participation \ue0 l\u2019entreprise de multiplication des semences communautaires et son utilisation par les producteurs comme une source de semences. Les entreprises de multiplication des semences communautaires ont \ue9t\ue9 identifi\ue9es rentables, g\ue9n\ue9rant des marges brutes de 792 US dollars et les semences de l\u2019haricot sont accessibles aux producteurs, compar\ue9es \ue0 d\u2019autres sources de semences, c\u2019est-\ue0-dire les syst\ue8mes semenciers formel et informel. Ces trois mod\ue8les de production et de distribution des semences se rivalisent \ue0 l\u2019\ue9chelle de l\u2019exploitation agricole, mais se compl\ue8tent en atteignant differents groupes sociaux d\u2019utilisateurs dans de localit\ue9s diff\ue9rentes. Les entreprises de multiplication des semences communautaires comme sources de semences, \ue9taient utilis\ue9es par les producteurs situ\ue9s dans les milieux ruraux et ceux en organisations/coop\ue9ratives paysannes. N\ue9amoins, la production des semences \ue0 travers ce mod\ue8le est plus concentr\ue9e dans les zones urbaines, o\uf9 les producteurs individuels de semences entrent facilement en contact avec le syst\ue8me semencier formel. Ceci, cependant met la commercialisation de la semence en \ue9troit contact avec les grands acheteurs pour la redistribution des semences entre les communaut\ue9s paysannes isol\ue9es