Insecticide Sensitivity of Native Chloride and Sodium Channels in a Mosquito Cell Line

The aim of this study was to investigate the utility of cultured Anopheles gambiae Sua1B cells for insecticide screening applications without genetic engineering or other treatments. Sua1B cells were exposed to the known insecticidal compounds lindane and DIDS, which inhibited cell growth at micromolar concentrations. In patch clamp studies, DIDS produced partial inhibition (69%) of chloride current amplitudes, and an IC50 of 5.1 μM was determined for Sua1B cells. A sub-set of chloride currents showed no response to DIDS; however, inhibition (64%) of these currents was achieved using a low chloride saline solution, confirming their identity as chloride channels. In contrast, lindane increased chloride current amplitude (EC50 = 116 nM), which was reversed when cells were bathed in calcium-free extracellular solution. Voltage-sensitive chloride channels were also inhibited by the presence of fenvalerate, a type 2 pyrethroid, but not significantly blocked by type 1 allethrin, an effect not previously shown in insects. Although no evidence of fast inward currents typical of sodium channels was observed, studies with fenvalerate in combination with veratridine, a sodium channel activator, revealed complete inhibition of cell growth that was best fit by a two-site binding model. The high potency effect was completely inhibited in the presence of tetrodotoxin, a specific sodium channel blocker, suggesting the presence of some type of sodium channel. Thus, Sua1B cells express native insect ion channels with potential utility for insecticide screening

Staatsbürgerschaftsregime im Wandel: oder: Die Gleichberechtigung wird zu Markte getragen Das Beispiel Kanada

The article presents a conceptualisation of 'citizenship regime' created out of a neo-institutionalist approach to political economy and then uses the concept to examine changes in the Canadian citizenship regime over time. The basic proposition is that if the postwar years were marked by regimelike discursive and practical coherence in a wide range of institutional connections between state and citizens, states' and citizens' responses to the economic and political conditions of the late 20th century are dismantling and reconstituting citizenship, so that the postwar regime exists no more

One in four die from acute infectious illness in an emergency department in Eastern Cape Province, South Africa

Background. Despite the breadth of data supporting evidence-based practice for sepsis care in high-resource settings, there are relatively few data to guide the management of sepsis in low-resource settings, particularly in areas where HIV and tuberculosis (TB) are prevalent. Furthermore, few studies had broadened sepsis parameters to include all patients with acute infectious illness or followed patients up after hospital discharge. Understanding the epidemiology and outcomes of acute infections in a local context is the critical first step to developing locally informed targeted management strategies.Objectives. To quantify and describe the incidence of and risk factors for mortality in a cohort of patients with undifferentiated acute infectious illnesses who presented to an emergency department (ED) in the Eastern Cape region of South Africa (SA).Methods. In this prospective cohort study, patients with suspected acute infectious illness were enrolled at a district casualty ward in Mthatha, SA, between 1 July and 1 September 2017. Demographic data, interventions, diagnostic studies and disposition were prospectively collected during the initial encounter and during the hospital stay. Follow-up was conducted both in hospital and via phone interviews 30 days after the index visit.Results. A total of 301 patients presented to the ED with acute infectious illness during the study period, of whom 54.8% had complete 30-day follow-up. Of the study population, only 5.7% had a complete set of vital signs (heart rate, respiratory rate, blood pressure and temperature) documented. Of the cohort, 51.8% had HIV and 32.9% active or treated TB; 25.2% of patients died within 30 days. Accounting for medical history, diagnosis and ED interventions, risk of mortality was independently associated with age (odds ratio (OR) 1.03; 95% confidence interval (CI) 1.00 - 1.06), HIV-positive status (OR 4.10; 95% CI 1.44 - 11.67) and Quick Sequential (Sepsis-Related) Organ Failure Assessment (qSOFA) score (OR 1.90; 95% CI 1.14 - 3.19) in an adjusted model. No ED interventions were protective for mortality, with intravenous fluid administration associated with increased 30-day mortality in this cohort (OR 3.65; 95% CI 1.38 - 9.62).Conclusions. Among adults with suspected acute infectious illness in Mthatha, SA, 30-day mortality was concerningly high. Mortality was highest in patients with concomitant HIV infection. In particular, vital sign assessment to identify possible sepsis in this cohort is crucial, as it affects mortality to a meaningful extent, yet is often unavailable. Future research is needed on the management of sepsis in low-resource settings, particularly in HIV-positive individuals

Development of Department Writing Guide for Civil Engineering

This paper describes the development of a writing guide for a civil engineering department. Motivation for developing a writing guide came from several sources. Freshmen enrolled in an introduction to civil engineering course turned in writing assignments demonstrating a need for improvement. The introductory course is frequently taken concurrently with a required freshman level writing class and well before a required discipline specific advanced writing class, so this was generally expected. Continued issues in junior and senior level classes, however, have clarified the need for additional program focus on written communication. Students have continually expressed frustration at having to adapt to varying lab report expectations from different faculty members and, most importantly, capstone design reports have demonstrated that student writing is not at industry expectations. The writing guide was a collaborative effort between civil engineering faculty and writing studies faculty. The initial phase focused on defining the content of the writing guide: reports (lab, project, etc.), memos, homework submittals, figures, tables, equations, professional e-mails, and references. The second phase was to develop an outline for the rubrics; the goal was for the rubrics to be general enough to be adapted by each faculty member for a given assignment, but still provide students with a consistent outline to assess their writing prior to submitting it for grade. Finally, in the third phase, the level of detail in the writing guide was discussed. In order to be useful, the writing guide was made specific enough for the students to use it to successfully complete writing assignments but general enough to allow individual faculty to adapt assignments toward the specific outcomes in each course. Above all else, the main goal of the writing guide is to prepare students for real world written communication. Therefore, it must not leave students with the impression that there is a template that can be applied regardless of audience. These concerns were considered during the development of the writing guide and will be part of in-class writing instruction within both civil engineering and writing courses. Written work will be assessed using both university and ABET assessment processes. Example work collected as part of the ABET process from the Fall 2012 semester will be retroactively assessed using the newly developed rubrics. In addition, Fall 2014 work will be assessed as it is submitted. Spring 2015 work will represent the first semester using the department writing guide. Pre-writing guide assessments will be compared to assessments of writing after the department guide is introduced. By comparing work over the next several years, senior year writing submittals will be used to determine if a greater level of competency was achieved by students exposed to the writing guide for their entire undergraduate experience as compared to students who received the writing guide late in their undergraduate career

Coming Together during COVID-19: A Mixed Methods Exploratory Study on Collective Efficacy in a State Developmental Disabilities Network

Background: Collective efficacy (CE) is a group’s shared belief that through their united efforts they can overcome challenges to achieve common goals (Bandura, 1993; 1997). CE has been shown to be related to professional growth, stress reduction, and overall collaborative impact in studies of groups responding to ongoing challenges as well as unforeseen circumstances (i.e. teachers, first-responders, and community responses to natural disasters) (Benight, 2004; Donohoo, 2016; Prati et al., 2011). COVID-19 has forced organizations serving individuals with disabilities to come together to adapt and change the ways in which they serve the disability community. Objective: This study examines reported attributes of CE as experienced by Arizona Developmental Disability Network (ADDN) members and their partners. This study respectively examines CE prior to COVID-19, currently, and predicted likelihood of CE attributes continuing in the future. Method: This mixed-method study involves data collected through a questionnaire and qualitative data collected through interviews with ADDN members and their partners. Results: The questionnaire results describe and compare the attributes of CE using sub-domains of social cohesion and trust, group competence, and enabling structures in three-time frames: prior to COVID-19, currently, and prediction of six months from now. The interviews provide narrative description of specific ADDN activities, roles, and perceptions; thus, adding a valuable dimension to interpreting the results. Conclusion: ADDN members and their partners have persisted and quickly adapted to COVID-19. These connections made during this time are likely to remain and help better serve Arizona’s disability community

Finding the Magnetic Center of a Quadrupole to High Resolution

In a companion pro, collposal it is proposed to align quadrupoles of a transport line to within transverse tolerances of 5 to 10 micrometers. Such a proposal is meaningful only if the effective magnetic center of such lenses can in fact be repeatably located with respect to some external mechanical tooling to comparable accuracy. It is the purpose of this note to describe some new methods and procedures that will accomplish this aim. It will be shown that these methods are capable of yielding greater sensitivity than the more traditional methods used in the past. The notion of the ''nodal'' point is exploited