Farmers’ preferences for cotton cultivation characteristics : a discrete choice experiment in Burkina Faso

While a fierce debate about the advantages and disadvantages of genetically modified crops is ongoing, it is surprising that farmers are often not consulted. In Burkina Faso, where insect resistant Bollgard II (R) cotton (further termed Bt cotton) was commercially released in 2008, studies highlight that cotton producers are in general satisfied with the reduction in insecticide use while the economic benefits are a source of controversy. To gain insight into farmers' preferences towards attributes in cotton cultivation, a discrete choice experiment (DCE) was developed. Five key attributes were identified to describe improved cotton varieties: seed development and provenance, seed costs, yield, required number of insecticide sprays, and preservation of agricultural practices. Farm-gate surveys were conducted among 324 cotton farmers in Western Burkina Faso. The results show that overall, farmers have a positive preference towards yield improvements and a negative preference towards pure private seed development and towards an increase in the requested number of insecticide applications or in the seed costs. According to their varieties at the time of the surveys (Bt and non-Bt), a difference was observed regarding their preferences for a status quo situation, indicating that those growing Bt had a stronger preference to keep the status quo than non-Bt farmers. When dividing the sample in segments based on the farm size, it was shown that there were different preferences with respect to the development of the variety and the required number of insecticide applications. Overall, it can be concluded from this study that economic benefits (linked to higher yields, lower seed costs, or reduced pesticide use) shape farmer's preferences

Interactions between the oomycete Pythium arrhenomanes and the rice root-knot nematode Meloidogyne graminicola in aerobic Asian rice varieties

Background: Aerobic rice fields are frequently infested by pathogenic oomycetes (Pythium spp.) and the rice root-knot nematode Meloidogyne graminicola. Here, the interaction between Pythium arrhenomanes and Meloidogyne graminicola was studied in rice roots of two aerobic rice varieties. In different experimental set-ups and infection regimes, plant growth, rice yield, Pythium colonization, as well as establishment, development and reproduction of M. graminicola were studied. Results: In this study, it is shown that the presence of P. arrhenomanes delays the establishment, development and reproduction of M. graminicola compared to single nematode infected plants. The delay in establishment and development of M. graminicola becomes stronger with higher P. arrhenomanes infection pressure. Conclusions: Our data indicate that P. arrhenomanes antagonizes M. graminicola in the rice root and that the plant benefits from this antagonism as shown by the yield data, especially when either of the pathogens is present in high levels

Mutant and chimeric recobinant plasminogen activatorsproduction in eukaryotic cellsand preliminary characterization

Mutant urokinase-type plasminogen activator (u-PA) genes and hybrid genes between tissue-type plasminogen activator (t-PA) and u-PA have been designed to direct the synthesis of new plasminogen activators and to investigate the structure-function relationship in these molecules. The following classes of constructs were made starting from cDNA encoding human t-PA or u-PA: 1) u-PA mutants in which the Arg156 and Lys158 were substituted with threonine, thus preventing cleavage by thrombin and plasmin; 2) hybrid molecules in which the NH2-terminal regions of t-PA (amino acid residues 1-67, 1-262, or 1-313) were fused with the COOH-terminal region of u-PA (amino acids 136-411, 139-411, or 195-411, respectively); and 3) a hybrid molecule in which the second kringle of t-PA (amino acids 173-262) was inserted between amino acids 130 and 139 of u-PA. In all cases but one, the recombinant proteins, produced by transfected eukaryotic cells, were efficiently secreted in the culture medium. The translation products have been tested for their ability to activate plasminogen after in situ binding to an insolubilized monoclonal antibody directed against urokinase. All recombinant enzymes were shown to be active, except those in which Lys158 of u-PA was substituted with threonine. Recombination of structural regions derived from t-PA, such as the finger, the kringle 2, or most of the A-chain sequences, with the protease part or the complete u-PA molecule did not impair the catalytic activity of the hybrid polypeptides. This observation supports the hypothesis that structural domains in t-PA and u-PA fold independently from one to another

A method to obtain disinfected Globodera infective juveniles directly from cysts

Les systèmes d'inoculation in vitro sont des outils performants et précis pour l'étude des interactions plantes-nématodes. L'obtention de juvéniles stériles est une étape cruciale pour la plupart de ces systèmes. La majorité des protocoles publiés comprennent une désinfection des juvéniles, ce qui conduit à une mortalité élevée. Nous décrivons ici une nouvelle méthode pour désinfecter, rapidement, facilement, et à faible coût des nématodes du genre #Globodera$, en partant de kystes. La mortalité des juvéniles désinfectés est faible (entre 10 et 40% au maximum). Les juvéniles stérilisés infestent les racines de pomme de terre cultivées in vitro et s'y développent normalement. (Résumé d'auteur

Magnetic moments of 68^{68}Cug,m^{g,m} and 70^{70}Cug,m1,m2^{g,m_{1},m_{2}} nuclei measured by in-source laser spectroscopy

We have obtained information on the atomic hyperfine splitting and, hence, on magnetic moments in neutron rich $^{68, 70}$Cu isotopes by scanning the frequency of the narrow-band laser of the first excitation step in the resonance ionization laser ion source. The deduced magnetic moments are $\mu( ^{68}$Cu$^{g}$, I$^{\pi}$ = 1$^+$) = +2.48(2)(7)$\mu_{N}$ ; $\mu(^{68}$Cu$^{m}$, I$^{\pi}$=6$^{-}$) = +1.24(4)(6)$\mu_{N}$ and $\mu(^{70}$Cu$^{m_{2}}$, I$^{\pi}$=1$^{+}$) = +1.86(4)(6)$\mu_{N}$ ; $\mu(^{70}$Cu$^{g}$, I$^{\pi}$=6$^{-}$) = +1.50(7)(8)$\mu_{N}$. The results of the scans analysis point out on existence of a new isomer in $^{70}$Cu$^{m_{1}}$. It's deduced magnetic moment is (-)3.50(7)(11)$\mu_{N}$ that is in a good agreement with I$^{\pi}$=3$^{-}$ assignment. The method of in-source atomic spectroscopy, as well as the analysis of the obtained data, is described. The results are discussed in terms of single-particle configurations coupled to the $^{68}$Ni core

Physical activity to improve cognition in older adults: can physical activity programs enriched with cognitive challenges enhance the effects? A systematic review and meta-analysis

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Dynamics of HIV-1 Assembly and Release

Assembly and release of human immunodeficiency virus (HIV) occur at the plasma membrane of infected cells and are driven by the Gag polyprotein. Previous studies analyzed viral morphogenesis using biochemical methods and static images, while dynamic and kinetic information has been lacking until very recently. Using a combination of wide-field and total internal reflection fluorescence microscopy, we have investigated the assembly and release of fluorescently labeled HIV-1 at the plasma membrane of living cells with high time resolution. Gag assembled into discrete clusters corresponding to single virions. Formation of multiple particles from the same site was rarely observed. Using a photoconvertible fluorescent protein fused to Gag, we determined that assembly was nucleated preferentially by Gag molecules that had recently attached to the plasma membrane or arrived directly from the cytosol. Both membrane-bound and cytosol derived Gag polyproteins contributed to the growing bud. After their initial appearance, assembly sites accumulated at the plasma membrane of individual cells over 1–2 hours. Assembly kinetics were rapid: the number of Gag molecules at a budding site increased, following a saturating exponential with a rate constant of ∼5×10−3 s−1, corresponding to 8–9 min for 90% completion of assembly for a single virion. Release of extracellular particles was observed at ∼1,500±700 s after the onset of assembly. The ability of the virus to recruit components of the cellular ESCRT machinery or to undergo proteolytic maturation, or the absence of Vpu did not significantly alter the assembly kinetics

A SNAP-Tagged Derivative of HIV-1—A Versatile Tool to Study Virus-Cell Interactions

Fluorescently labeled human immunodeficiency virus (HIV) derivatives, combined with the use of advanced fluorescence microscopy techniques, allow the direct visualization of dynamic events and individual steps in the viral life cycle. HIV proteins tagged with fluorescent proteins (FPs) have been successfully used for live-cell imaging analyses of HIV-cell interactions. However, FPs display limitations with respect to their physicochemical properties, and their maturation kinetics. Furthermore, several independent FP-tagged constructs have to be cloned and characterized in order to obtain spectral variations suitable for multi-color imaging setups. In contrast, the so-called SNAP-tag represents a genetically encoded non-fluorescent tag which mediates specific covalent coupling to fluorescent substrate molecules in a self-labeling reaction. Fusion of the SNAP-tag to the protein of interest allows specific labeling of the fusion protein with a variety of synthetic dyes, thereby offering enhanced flexibility for fluorescence imaging approaches