Incorporation of cytochrome oxidase into cardiolipin bilayers and induction of nonlamellar phases.

Cytochrome oxidase from beef heart has been lipid-substituted with beef heart cardiolipin. The lipid phase behavior and protein aggregation state of the reconstituted complexes have been studied with 31P NMR, freeze-fracture electron microscopy, and saturation-transfer ESR of the spin-labeled protein. In the absence of salt, the lipid has a lamellar arrangement, and the protein is integrated and uniformly distributed in the membrane vesicles and undergoes rapid rotational diffusion. The presence of the protein stabilizes the cardiolipin lamellar phase against salt-induced transitions to the inverted hexagonal phase. The threshold salt concentration becomes higher and the extent of conversion becomes lower with decreasing lipid:protein ratio. In high salt, lamellar-phase lipid with integrated protein coexists with hexagonal-phase lipid free of protein, and the rotational diffusion of the protein is drastically reduced as a result of the high packing density

Learning from the experts: exploring playground experience and activities using a write and draw technique.

BACKGROUND: Qualitative research into the effect of school recess on children's physical activity is currently limited. This study used a write and draw technique to explore children's perceptions of physical activity opportunities during recess. METHODS: 299 children age 7-11 years from 3 primary schools were enlisted. Children were grouped into Years 3 & 4 and Years 5 & 6 and completed a write and draw task focusing on likes and dislikes. Pen profiles were used to analyze the data. RESULTS: Results indicated 'likes' focused on play, positive social interaction, and games across both age groups but showed an increasing dominance of games with an appreciation for being outdoors with age. 'Dislikes' focused on dysfunctional interactions linked with bullying, membership, equipment, and conflict for playground space. Football was a dominant feature across both age groups and 'likes/dislikes' that caused conflict and dominated the physically active games undertaken. CONCLUSION: Recess was important for the development of conflict management and social skills and contributed to physical activity engagement. The findings contradict suggestions that time spent in recess should be reduced because of behavioral issues

Spectrophotovoltaic orbital power generation

A system with 1000 : 1 concentration ratio is defined, using a cassegrain telescope as the first stage concentration (270 x) and compound parabolic concentrators (CPC) for the second stage concentration of 4.7 x for each spectral band. Using reported state of the art (S.O.A.) solar cells device parameters and considering structural losses due to optics and beamsplitters, the efficiencies of one to four cell systems were calculated with efficiencies varying from approximately 22% to 30%. Taking into account cost of the optics, beamsplitter, radiator, and the cost of developing new cells the most cost effective system is the GaAs/Si system

Exploring the roles of physical effort and visual salience within the proximity effect

Background Recent work has explored the effectiveness of the Proximity Effect, where increasing the physical distance between consumer and snacks reduces intake. Foods requiring less effort to attain, or being more visually appealing, are seen to be consumed more. Relatedly, perceived effort and visual salience are suggested mechanisms for the proximity effect, but no prior studies have directly manipulated these in association with the effect. Two between-subjects studies conducted in university laboratories are presented. Method Twenty chocolate brownies that were either wrapped or unwrapped (Study 1, N = 85), or 250g of M&M's, either colourful or plain brown (Study 2, N = 80), were presented as effort and salience manipulations respectively to participants at either 20 cm or 70 cm. Consumption was measured as ‘likelihood of consumption’ (Yes/No) and ‘actual consumption’ (units/grams). Potential moderating variables including perceived effort and perceived visual salience were also measured. Results Likelihood of consumption was positively predicted by perceived visual salience in both Studies, and by distance in Study 2. Significant main effects of distance, p  70 cm), effort, p  wrapped), and distance × effort interaction, p = .003, ȵ2 = 0.111, were observed in Study 1 for actual consumption. A main effect of distance was found in Study 2 for actual consumption, p  70 cm). Perceived visual salience positively correlated with actual consumption in both Studies. Conclusions Increasing physical effort and placing snacks further away appear to act independently and interactively to reduce snack consumption. Manipulating snack colour does not appear to influence consumption, whereas perceptions of visual salience appear to influence consumption. As such, perceived visual salience and physical effort are thought to be key mechanisms underpinning the proximity effect

Exploring the underpinning mechanisms of the proximity effect within a competitive food environment

Objective: One method of influencing an individual's food consumption involves placing unhealthy snacks further away from individuals, known as the “proximity effect”. However, only one laboratory study has explored the effect while both an unhealthy and a healthy option are presented simultaneously. Further, little is known about the potential underpinning mechanisms of the effect. The current study aims to replicate the proximity effect in a competitive environment, and to explore the role of visual salience and effort in the proximity effect. Method: Fifty-six participants were asked to complete a two-part questionnaire under the cover story of a relaxation study. Two bowls were presented to participants, each containing either 250 g chocolate M&M's or 250 g mixed fruit pieces. Each bowl was positioned either 20 cm or 70 cm from the participant, creating four proximity conditions. Consumption of each snack was compared between proximity conditions. Results: No main effects were found. A significant interaction between snack type and chocolate position was found (p = .010, ȵ2 = 0.159), with fruit consumption being significantly higher when chocolate was at located at 20 cm compared to 70 cm (53.35 g vs 22.35 g, p = .042). Higher visual salience of each snack type correlated to more of the snack being consumed, ps < .017. Results were similar when calories consumed were analysed. Conclusions: We found an unconventional proximity effect where the consumption of a snack did not depend on its position, but rather the relative position of another snack. Implications of the study could inform café and supermarket layouts to exploit the interaction between moving healthy items closer in addition to moving unhealthy items further away, in order to maximise choice of healthy item

Lipid-protein interactions in stacked and destacked thylakoid membranes and the influence of phosphorylation and illumination. Spin label ESR studies.

The effects of membrane destacking, protein phosphorylation, and continuous illumination have been studied in pea thylakoid membranes using ESR spectroscopy of an incorporated spin-labelled phosphatidylglycerol. This spin-labelled analogue of an endogenous thylakoid lipid has previously been shown to exhibit a selectivity of interaction with thylakoid proteins. Neither destacking, phosphorylation nor illumination was found to change the ESR spectra appreciably, suggesting that for phosphatidylglycerol at least, neither the number of protein-associated membrane lipids nor their pattern of selectivity was altered. The redistribution of the thylakoid protein complexes in the membrane, under these various conditions, therefore takes place with conservation of the properties of the lipid/protein interface

The Sources of b-Quarks at the Tevatron and their Correlations

The leading-log order QCD hard scattering Monte-Carlo models of HERWIG, ISAJET, and PYTHIA are used to study the sources of b-quarks at the Tevatron. The reactions responsible for producing b and bbar quarks are separated into three categories; flavor creation, flavor excitation, and parton-shower/fragmentation. Flavor creation corresponds to the production of a b-bbar pair by gluon fusion or by annihilation of light quarks, while flavor excitation corresponds to a b or bbar quark being knocked out of the initial-state by a gluon or a light quark or antiquark. The third source occurs when a b-bbar pair is produced within a parton shower or during the fragmentation process of a gluon or a light quark or antiquark (includes gluon splitting). The QCD Monte-Carlo models indicate that all three sources of b-quarks are important at the Tevatron and when combined they qualitatively describe the inclusive cross-section data. Correlations between the b and bbar quark are very different for the three sources and can be used to isolate the individual contributions.Comment: RevTex4, 14 pages, 20 figures, submitted to Phys. Rev.

A large sample study of spin relaxation and magnetometric sensitivity of paraffin-coated Cs vapor cells

We have manufactured more than 250 nominally identical paraffin-coated Cs vapor cells (30 mm diameter bulbs) for multi-channel atomic magnetometer applications. We describe our dedicated cell characterization apparatus. For each cell we have determined the intrinsic longitudinal, \sGamma{01}, and transverse, \sGamma{02}, relaxation rates. Our best cell shows \sGamma{01}/2\pi\approx 0.5 Hz, and \sGamma{02}/2\pi\approx 2 Hz. We find a strong correlation of both relaxation rates which we explain in terms of reservoir and spin exchange relaxation. For each cell we have determined the optimal combination of rf and laser powers which yield the highest sensitivity to magnetic field changes. Out of all produced cells, 90% are found to have magnetometric sensitivities in the range of 9 to 30 fTHz. Noise analysis shows that the magnetometers operated with such cells have a sensitivity close to the fundamental photon shot noise limit

A recombinant bovine herpesvirus-4 vectored vaccine delivered via intranasal nebulization elicits viral neutralizing antibody titers in cattle

Recombinant herpesvirus vaccine vectors offer distinct advantages in next-generation vaccine development, primarily due to the ability to establish persistent infections to provide sustainable antigen responses in the host. Recombinant bovine herpesvirus-4 (BoHV-4) has been previously shown to elicit protective immunity in model laboratory animal species against a variety of pathogens. For the first time, we describe the induction of antigen-specific immune responses to two delivered antigens in the host species after intranasal nebulization of recombinant BoHV-4 expressing the chimeric peptide containing the bovine viral diarrhea virus (BVDV) glycoprotein E2 and the bovine herpesvirus 1 (BoHV-1) glycoprotein D (BoHV-4-A-CMV-IgK-gE2gD-TM). In this study, four cattle were immunized via intranasal nebulization with the recombinant BoHV-4 construct. Two of the cattle were previously infected with wild-type BoHV-4, and both developed detectable serologic responses to BVDV and BoHV-1. All four immunized cattle developed detectable viral neutralizing antibody responses to BVDV, and one steer developed a transient viral neutralizing response to BoHV-1. Approximately one year after immunization, immunosuppressive doses of the glu-cocorticoid dexamethasone were administered intravenously to all four cattle. Within two weeks of immunosuppression, all animals developed viral neutralizing antibody responses to BoHV-1, and all animals maintained BVDV viral neutralizing capacity. Overall, nebulization of BoHV-4-A-CMV-IgK-gE2gD-TM persistently infects cattle, is capable of eliciting antigen-specific immunity following immunization, including in the presence of pre-existing BoHV-4 immunity, and recrudescence of the virus boosts the immune response to BoHV-4-vectored antigens. These results indicate that BoHV-4 is a viable and attractive vaccine delivery platform for use in cattle